Article
Food Science & Technology
Sebastian Diechler, Bianca E. Chichirau, Gernot Posselt, Dionyssios N. Sgouras, Silja Wessler
Summary: Research has shown that the diverse structure of CagA regulates B cell physiology, while B cell survival is independent of CagA.
Article
Microbiology
Kavinda Tissera, Myeong-A Kim, Jing Lai, Sacheera Angulmaduwa, Aeryun Kim, D. Scott Merrell, Ji-Hye Kim, Hanfu Su, Jeong-Heon Cha
Summary: The CagA protein of H. pylori shows geographic-dependent variation in EPIYA motifs, with East-Asian isolates carrying ABD type and Western isolates carrying ABC type. Some Western isolates exhibit multi-copy EPIYA-C motifs which are associated with disease severity. In a study of Korean clinical isolates, some East-Asian isolates were found to carry Western ABC-type CagA, indicating possible intraspecies diversity in East-Asian H. pylori.
JOURNAL OF MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Tohid Piri-Gharaghie, Ghazal Ghajari, Shakiba Tolou-Shikhzadeh-Yazdi, Mona Aghassizadeh-Sherbaf, Sahar Khorsand-Dehkordi
Summary: Despite being a serious pathogen, the origin of Helicobacter pylori (H. pylori) remains unknown. Poultry, such as chicken and turkey, is widely consumed as a source of protein worldwide, making it important to ensure sanitary practices in poultry delivery for food. This study examined the distribution of pathogenicity and antimicrobial resistance in H. pylori isolates from poultry meat. The findings highlight the presence of H. pylori in raw poultry samples and the prevalence of antibiotic-resistant genotypes, raising concerns about public health risks associated with consuming raw poultry.
SCIENTIFIC REPORTS
(2023)
Article
Infectious Diseases
Mengheng Mi, Fangcao Wu, Jian Zhu, Fang Liu, Guzhen Cui, Xueqing Wen, Yue Hu, Zhaohui Deng, Xiaojuan Wu, Zhengrong Zhang, Tingna Qi, Zhenghong Chen
Summary: The research revealed the presence of heterogeneous Helicobacter pylori strains with resistance to different drugs and virulence within the gastric mucosa of individual patients. Genetic diversity among monoclonal strains influenced antibiotic susceptibility and some strains exhibited genotype variations impacting treatment efficacy.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Food Science & Technology
Aina Ichihara, Hinako Ojima, Kazuyoshi Gotoh, Osamu Matsushita, Susumu Take, Hiroyuki Okada, Akari Watanabe, Kenji Yokota
Summary: This study aimed to explore the association between different antibody reactivities and bacterial genome organization. High-reactive strains showed high gene homology, while inversion around the vacA gene was found in the genome of poorly responsive antigen strains.
Review
Biochemistry & Molecular Biology
Silvia Salvatori, Irene Marafini, Federica Laudisi, Giovanni Monteleone, Carmine Stolfi
Summary: H. pylori infection contributes to the onset and development of gastric cancer through the mechanisms of chronic inflammation and DNA damage to gastric epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Yoshiaki Usui, Yukari Taniyama, Mikiko Endo, Yuriko N. Koyanagi, Yumiko Kasugai, Isao Oze, Hidemi Ito, Issei Imoto, Tsutomu Tanaka, Masahiro Tajika, Yasumasa Niwa, Yusuke Iwasaki, Tomomi Aoi, Nozomi Hakozaki, Sadaaki Takata, Kunihiko Suzuki, Chikashi Terao, Masanori Hatakeyama, Makoto Hirata, Kokichi Sugano, Teruhiko Yoshida, Yoichiro Kamatani, Hidewaki Nakagawa, Koichi Matsuda, Yoshinori Murakami, Amanda B. Spurdle, Keitaro Matsuo, Yukihide Momozawa
Summary: This study reveals that certain genetic variants associated with Helicobacter pylori infection can increase the risk of gastric cancer. Individuals carrying these genetic variants and infected with H. pylori have an even higher risk of developing gastric cancer.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Microbiology
Oyunbaatar Altanbayar, Avarzed Amgalanbaatar, Chimeddorj Battogtokh, Narmandakh Bayarjargal, Dana Belick, Colin R. Mackenzie, Klaus Pfeffer, Birgit Henrich, Malte Kohns Vasconcelos
Summary: Helicobacter pylori infection is strongly associated with gastritis, gastroduodenal ulcer disease, and gastric carcinoma. This study characterized the cagA gene in H. pylori strains from Mongolian patients with gastritis or ulcer. The results showed that most strains carried the Western type of CagA, while only one strain had the East Asian type. Additionally, an amino acid exchange in the EPIYA-B motif of CagA proteins was found in strains with fewer EPIYA-C copies. These findings suggest that Mongolian H. pylori strains with CagA proteins containing both multiple EPIYA-A segments and fewer EPIYA-C segments may have lower virulence.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Xiujuan Xia, Linfang Zhang, Hao Wu, Feng Chen, Xuanyou Liu, Huifang Xu, Yuqi Cui, Qiang Zhu, Meifang Wang, Hong Hao, De-Pei Li, William P. Fay, Luis A. Martinez-Lemus, Michael A. Hill, Canxia Xu, Zhenguo Liu
Summary: This study found that CagA(+) H. pylori effectively colonizes gastric mucosa and impairs endothelial function, leading to increased atherosclerosis through exosomes-mediated ROS formation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Infectious Diseases
Tomomi Kobatake, Keiki Ogino, Hiroyuki Sakae, Kazuyoshi Gotoh, Akari Watanabe, Osamu Matsushita, Hiroyuki Okada, Kenji Yokota
Summary: The study found that disulfiram has bactericidal effects on Helicobacter pylori at low concentrations, suggesting it can serve as a supplement to current H. pylori eradication drugs.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Medicine, General & Internal
Mohamed R. Jouimyi, Ghizlane Bounder, Hasna Boura, Imane Essaidi, Asmaa Bendahmane, Hakima Benomar, Khalid Zerouali, Halima Lebrazi, Anass Kettani, Valerie C. Gbonon, Maachi Fatima
Summary: This study evaluated the association of EPIYA motifs with the risk of AG and IM in the Casablanca population. The results showed that patients with the EPIYA-ABCC motif were more likely to develop IM. Therefore, the EPIYA-ABCC motif may be a useful marker for identifying patients at high risk of developing IM that can lead to GC.
AFRICAN HEALTH SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Zhijing Xue, Yuanhai You, Lihua He, Yanan Gong, Lu Sun, Xiurui Han, Ruyue Fan, Kangle Zhai, Yaming Yang, Maojun Zhang, Xiaomei Yan, Jianzhong Zhang
Summary: In this study, 503 CagA sequences were analyzed, revealing that most H. pylori strains in the Chinese population were of the CagA-ABD subtype, which was associated with gastroduodenal diseases. Amino acid polymorphisms at residues 893 and 894 flanking the EPIYA motifs were found to have a statistically significant association with gastric cancer.
Article
Medicine, Research & Experimental
Zong-Bao Yan, Jin-Yu Zhang, Yi-Pin Lv, Wen-Qing Tian, Zhi-Guo Shan, Fang-Yuan Mao, Yu-Gang Liu, Wan-Yan Chen, Pan Wang, Yun Yang, Ping Cheng, Liu-Sheng Peng, Ya-Ling Liao, Geng-Yu Yue, Xiao-Lin Xu, Yong-Liang Zhao, Mu-Han Lu, Yuan Zhuang
Summary: The study found that REDD1 is increased in the gastric mucosa of H. pylori-infected patients and mice. H. pylori induced GECs to express REDD1 via the phosphorylated cagA, activating the MAPKp38 pathway. In Redd1-/- mice, gastric inflammation, MHCII+ monocyte infiltration, IL-23, and IL-17A were all attenuated.
Article
Multidisciplinary Sciences
Bui Hoang Phuc, Vo Phuoc Tuan, Tran Thanh Binh, Pham Huu Tung, Tran Dinh Tri, Ho Dang Quy Dung, Ngo Phuong Minh Thuan, Kartika Afrida Fauzia, Evariste Tshibangu-Kabamba, Ricky Indra Alfaray, Batsaikhan Saruuljavkhlan, Takashi Matsumoto, Junko Akada, Yoshio Yamaoka
Summary: This study reports the complete genomes of two Helicobacter pylori strains, CHC155 and VN1291, isolated from a non-cardia gastric cancer patient and a duodenal ulcer patient, respectively, and demonstrates their virulence through in vitro infection. The genomes of both strains possess four kinds of genomic islands related to H. pylori pathogenicity. These genomes also carry genes associated with antibiotic resistance, which are risk factors in the development of gastric cancer and duodenal ulcer.
SCIENTIFIC REPORTS
(2023)
Article
Gastroenterology & Hepatology
Sungil Jang, Lori M. Hansen, Hanfu Su, Jay Solnick, Jeong-Heon Cha
Summary: This study found that the number of cagA copies in Helicobacter pylori is higher in mice with less intense immune response and lower in mice with more intense immune response. Additionally, cagY recombination is also associated with H. pylori virulence.
Article
Microbiology
Kiarash Ghazvini, Yoshio Yamaoka, Mohsen Karbalaei, Masoud Keikha
Summary: This study aims to assess the relationship between H. pylori infection and alopecia areata, and found a significant association between H. pylori infection and susceptibility to alopecia areata.
REVIEWS AND RESEARCH IN MEDICAL MICROBIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Adi Wasis Prakosa, Muhammad Miftahussurur, Juniastuti Juniastuti, Langgeng Agung Waskito, Dalla Doohan, Kartika Afrida Fauzia, Yudith Annisa Ayu Rezkitha, Titong Sugihartono, Ari Fahrial Syam, Tomohisa Uchida, Yoshio Yamaoka
DIGESTIVE DISEASES
(2022)
Editorial Material
Microbiology
Filipa F. Vale, Philippe Lehours, Yoshio Yamaoka
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Osman Aksoy, Judith Lind, Vincent Sunder-Plamann, Sonia Vallet, Klaus Podar
Summary: In Multiple Myeloma (MM), the disruption of the bone marrow microenvironment leads to the evasion of apoptosis by cancer cells. The MM BM milieu, consisting of cytokines, growth factors, intercellular and cell: ECM interactions, and exosomes, contributes to the abundance of pro-survival Bcl-2 family members in tumor cells. Advances in molecular knowledge have resulted in the development of small molecules that mimic BH3-only proteins, which show promise as therapeutic strategies to target Bcl-2 family members. Modifying the tumor microenvironment to indirectly activate apoptotic pathways is also being explored. This review summarizes our current understanding of the molecular mechanisms underlying MM tumor cell evasion from apoptosis, as well as potential therapeutic strategies to overcome Bcl-2-mediated tumor cell survival.
Article
Biochemistry & Molecular Biology
Johannes Luppe, Heinrich Sticht, Francois Lecoquierre, Alice Goldenberg, Kathleen M. Gorman, Ben Molloy, Emanuele Agolini, Antonio Novelli, Silvana Briuglia, Outi Kuismin, Carlo Marcelis, Antonio Vitobello, Anne-Sophie Denomme-Pichon, Sophie Julia, Johannes R. Lemke, Rami Abou Jamra, Konrad Platzer
Summary: The study identified eight individuals with ultra rare variants in STX1A who presented with a spectrum of intellectual disability, autism, and epilepsy. The phenotypic course varied depending on the type of variant, with missense variants mainly causing epilepsy and single amino acid deletions and the splice variant causing intellectual disability and autistic behavior. In silico modeling showed different impaired protein-protein interactions for missense variants and single amino acid deletions. These findings suggest two different pathogenic mechanisms underlying STX1A-related neurodevelopmental disorders.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Ella Vintschger, Dennis Kraemer, Pascal Joset, Anselm H. C. Horn, Anita Rauch, Heinrich Sticht, Ruxandra Bachmann-Gagescu
Summary: Next generation sequencing (NGS) is used to detect carrier status for rare recessive disorders. The recent ACMG recommendations support NGS-based carrier screening (NGS-CS) for genes with a carrier frequency > 1/200. However, variant interpretation in healthy individuals presents challenges, and the true carrier frequencies are uncertain. Thus, genetic counseling for NGS-CS should disclose these limitations.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Paolo Zanoni, Katharina Steindl, Heinrich Sticht, Beatrice Oneda, Pascal Joset, Ivan Ivanovski, Anselm H. C. Horn, Elena. M. M. Cabello, Julia Laube, Markus Zweier, Alessandra Baumer, Anita Rauch, Nadia Khan
Summary: In this study, a comprehensive genetic and clinical analysis was conducted on a large cohort of pediatric MMA patients. RNF213 and neurofibromatosis type 1 (NF1) were identified as the two main subgroups. Patients with deleterious RNF213 variants exhibited severe clinical manifestations, early symptom onset, and a high rate of multiple territory strokes. NF1 patients had similar stroke burden compared to non-NF1 individuals and were often incidentally diagnosed during routine MRIs. The study also found that MMA-associated RNF213 variants had lower functional impact compared to those associated with aortic disease. The possible association of MMA with recurrent chromosomal imbalances and STAT3 deficiency was also discussed.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Immunology
Urszula Zarzecka, Nicole Tegtmeyer, Heinrich Sticht, Steffen Backert
Summary: The pathogen Helicobacter pylori is a major risk factor for gastric diseases. The serine protease HtrA is a virulence factor that causes gastric tissue damage by cleaving cell junction proteins. This study found that the stability of HtrA trimer varies in different clinical strains of H. pylori and is correlated with the presence of leucine or serine residue at position 171. The natural polymorphism at this position may affect HtrA protease activity during infection and contribute to gastric disease development.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Marcus Conrad, Anselm H. C. Horn, Heinrich Sticht
Summary: Through molecular dynamics simulations, it was found that the tautomers and charge states of histamine have a significant impact on its interaction with the histamine H1 receptor. The tau-tautomer formed stable interactions with the receptor, while the pi-tautomer only formed weaker hydrogen bonding interactions. Additionally, the dicationic histamine showed weaker interaction with the receptor and induced an inactive conformation in the absence of the G(q) protein. These findings have implications for the development of new ligands that stabilize specific activation states of the H1 receptor.
Article
Biochemistry & Molecular Biology
Suneesh Kumar Pachathundikandi, Nicole Tegtmeyer, Steffen Backert
Summary: The given passage is too short for summarization.
TRENDS IN MICROBIOLOGY
(2023)
Article
Biology
Manuel Deubler, Lucas Weissenborn, Simon Leukel, Anselm H. C. Horn, Jutta Eichler, Heinrich Sticht
Summary: PG16 is a broadly neutralizing antibody that binds to the gp120 subunit of the HIV-1 Env protein. Sulfation of Tyr100H in the CDRH3 residue enhances interactions with gp120 and stabilizes the protein-protein contacts and interactions with the gp120 glycan shield. PG16-CDRH3 can be used as a template to develop peptide mimetics as potential inhibitors of HIV invasion.
Article
Sport Sciences
Adi Pranoto, Maulana Bagus Adi Cahyono, Reinaldi Yakobus, Nabilah Izzatunnisa, Roy Novri Ramadhan, Purwo Sri Rejeki, Muhammad Miftahussurur, Wiwin Is Effendi, Citrawati Dyah Kencono Wungu, Yoshio Yamaoka
Summary: A sedentary lifestyle and unhealthy diet increase the risk of obesity, which leads to the production of proinflammatory cytokines and higher morbidity and mortality rates. This study examined the effects of different types of exercise on reducing proinflammatory cytokines in young adult females with obesity.
Article
Plant Sciences
Theresa Maria Reimann, Christina Muedsam, Christina Schachtler, Semra Ince, Heinrich Sticht, Christian Herrmann, Michael Stuerzl, Benedikt Kost
Summary: In this study, the Arabidopsis GBP orthologue AtGBPL3 was identified as a crucial component in mitotic nuclear envelope reformation, nuclear morphogenesis, and transcriptional repression during interphase. AtGBPL3 preferentially accumulates at the nuclear envelope in mitotically active root tips and interacts with centromeric chromatin and lamina components, leading to transcriptional repression of pericentromeric chromatin. Reduced expression of AtGBPL3 or associated lamina components results in altered nuclear morphology and overlapping transcriptional deregulation.
Article
Biochemistry & Molecular Biology
Martin Schuetz, Regina Mueller, Eileen Socher, Christina Wangen, Florian Full, Emanuel Wyler, Diana Wong, Myriam Scherer, Thomas Stamminger, Sunwen Chou, William D. Rawlinson, Stuart T. Hamilton, Heinrich Sticht, Manfred Marschall
Summary: The complex host interaction network of human cytomegalovirus (HCMV) involves the regulatory protein kinase pUL97 and human cyclins. The study found that cyclin H is a crucial determinant of pUL97 kinase activity and viral replication efficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Environmental Sciences
Masoud Keikha, Amirhossein Sahebkar, Yoshio Yamaoka, Mohsen Karbalaei
Summary: This study found that infection with cagA-positive H.pylori strains does not have a significant effect on the formation of gastric MALT lymphoma, while translocated CagA antigen plays a crucial role in the development of diffuse large B-cell lymphoma. Additionally, there is an inverse association between vacA and gastric MALT lymphoma risk.
JOURNAL OF HEALTH POPULATION AND NUTRITION
(2022)