Article
Multidisciplinary Sciences
Yoshinori Kawabe, Masamichi Kamihira
Summary: This study analyzed the phenotypic changes, gene expression profiles, and karyotype of immortalized kidney cells (CHK-Q) derived from Chinese hamster tissue. The results suggest that CHK-Q cells may have transformed from renal stem cells and the immortalization process is associated with increased fluctuations in the expression of specific proto-oncogenes.
Article
Veterinary Sciences
Dongjin Oh, Hyerin Choi, Mirae Kim, Lian Cai, Joohyeong Lee, Ali Jawad, Sohee Kim, Haomiao Zheng, Gabsang Lee, Yubyeol Jeon, Sang-Hwan Hyun
Summary: This study demonstrated the localization of IL-7 and IL-7R in porcine embryos during in vitro culture and suggested that IL-7 supplementation can enhance embryonic development and blastocyst quality.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Cell Biology
Federica Fantuzzi, Sanna Toivonen, Andrea Alex Schiavo, Heeyoung Chae, Mohammad Tariq, Toshiaki Sawatani, Nathalie Pachera, Ying Cai, Chiara Vinci, Enrico Virgilio, Laurence Ladriere, Mara Suleiman, Piero Marchetti, Jean-Christophe Jonas, Patrick Gilon, Decio L. Eizirik, Mariana Igoillo-Esteve, Miriam Cnop
Summary: The study comprehensively characterized the functionality of iPSC-derived beta cells both in vitro and in vivo in humanized mice. Differentiation in microwells showed equal efficiency with rotating suspension, but with a higher success rate. In vivo transplanted beta cells demonstrated functional characteristics similar to human islets, highlighting the potential of generating islet-like organoids for diabetes research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Microbiology
Shakirat A. Adetunji, Dmitriy Smolensky, Dana N. Mitzel, Jeana L. Owens, Carol G. Chitko-McKown, Natalia Cernicchiaro, Leela E. Noronha
Summary: This study identified several porcine cell lines susceptible to JEV infection, which could serve as valuable research tools for investigating the dynamics of JEV infection and host cell mechanisms.
Article
Biochemistry & Molecular Biology
Alla S. Koltsova, Olga A. Efimova, Olga V. Malysheva, Natalia S. Osinovskaya, Thomas Liehr, Ahmed Al-Rikabi, Natalia Yu. Shved, Iskender Yu. Sultanov, Olga G. Chiryaeva, Maria I. Yarmolinskaya, Nikolai I. Polenov, Vladislava V. Kunitsa, Maka I. Kakhiani, Tatyana G. Tral, Gulrukhsor Kh. Tolibova, Olesya N. Bespalova, Igor Yu. Kogan, Andrey S. Glotov, Vladislav S. Baranov, Anna A. Pendina
Summary: Uterine leiomyomas (ULs) exhibit both inter- and intratumor genetic heterogeneity. Regardless of the MED12 status, a tumor may consist of clones with and without chromosomal abnormalities. Under in vitro conditions, the clonal frequency of cells shifts up or down, possibly due to variations in the ability of cells with different genetic aberrations to survive outside the body.
Article
Developmental Biology
Mattias Malaguti, Rosa Portero Migueles, Jennifer Annoh, Daina Sadurska, Guillaume Blin, Sally Lowell
Summary: Cell-cell interactions play a crucial role in early development, but studying these processes is challenging due to a lack of efficient analysis tools. In this study, we introduce a new technology called SyNPL, which allows us to investigate cell-cell interactions in pluripotent cells and chimeric embryos. The system utilizes engineered "sender" and "receiver" cells to report these interactions and can be customized to program differentiation decisions and promote neuronal differentiation.
Article
Cell Biology
Xuechun Li, Yang Yu, Renyue Wei, Yimei Li, Jiawei Lv, Zhonghua Liu, Yu Zhang
Summary: PSCs are a promising source of ECs for cardiovascular disease treatment due to ethical concerns and immune rejection risks associated with ESCs. iPSCs offer a solution for these issues. Swine serve as a suitable model for human, but there is limited research on EC differentiation of piPSCs. This study successfully differentiated piPSCs into ECs and demonstrated their functional properties, showing potential for cell transplantation and mechanistic studies in endothelial dysfunction.
Article
Veterinary Sciences
Takato Takenouchi, Kentaro Masujin, Shunichi Suzuki, Seiki Haraguchi, Kanae Hiramatsu, Takehiro Kokuho, Hirohide Uenishi
Summary: Mononuclear phagocytes (MNP), including monocytes, dendritic cells (DC), and macrophages, play critical roles in innate immunity. In this study, scientists established a cell line derived from porcine lungs called immortalized PLuM (IPLuM), which shows characteristics of DC/macrophages and demonstrates activity against inflammatory cytokines and bacteria. Furthermore, the IPLuM cell line is susceptible to African swine fever virus infection.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Cell Biology
M. Juliana Gomez-Garcia, Elya Quesnel, Rasha Al-attar, Andrew R. Laskary, Michael A. Laflamme
Summary: This review discusses the immature phenotype of human pluripotent stem cell derived cardiomyocytes (hPSC-CMs) and potential strategies for their maturation, considering the developmental processes and signaling cues involved in normal human cardiac maturation. Various approaches for hPSC-CM maturation, such as prolonged culture duration, metabolic manipulation, and tissue engineering, are summarized, along with evidence of maturation post-implantation in injured hearts and its implications for potential hPSC-based cardiac therapy.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Kaituo Liu, Ming Zhao, Yan Li, Liang Luo, Dahai Hu
Summary: The VEGF hydrogel significantly accelerates wound healing and promotes collagen deposition and the formation of new blood vessels. It retains the biological activity of VEGF and has the potential to be an attractive biomaterial for clinical applications.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2022)
Article
Cell Biology
Joohyeong Lee, Lian Cai, Mirae Kim, Hyerin Choi, Dongjin Oh, Ali Jawad, Eunsong Lee, Sang-Hwan Hyun
Summary: This study confirms the effectiveness of blastomere aggregation using phytohemagglutinin-L (PHA-L) in improving the developmental competence and quality of porcine embryos. It also demonstrates that PHA-L treatment enhances the efficiency of embryonic stem cell establishment and embryoid-body formation. This technology has the potential to improve embryo quality, increase the success of embryonic stem cell culture, and be used in chimeric animal production and other applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Veterinary Sciences
Cristina Cuello, Cristina A. Martinez, Josep M. Cambra, Alejandro Gonzalez-Plaza, Inmaculada Parrilla, Heriberto Rodriguez-Martinez, Maria A. Gil, Emilio A. Martinez
Summary: The study showed that vitrification altered the gene expression pattern of porcine morulae produced in vivo, leading to disruptions in metabolic pathways that may interfere with embryo quality and developmental potential.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Vera A. van der Weijden, Meret Schmidhauser, Mayuko Kurome, Johannes Knubben, Veronika L. Floter, Eckhard Wolf, Susanne E. Ulbrich
Summary: Our study found that in vivo developed and in vitro produced embryos show similar transcriptome profiles during development. Shared canonical pathways in transitions from 4-cell to morula included oxidative phosphorylation and EIF2 signaling, while shared pathways from morula to hatched blastocyst included 14-3-3-mediated signaling, xenobiotic metabolism general signaling pathway, and NRF2-mediated oxidative stress response. Hatched blastocysts from in vitro fertilization-pipeline exhibited molecular pathways associated with lower developmental competence compared to in vivo developed embryos.
Article
Pharmacology & Pharmacy
Hongyu Rao, Weiwei Su, Xiaoli Zhang, Yonggang Wang, Tao Li, Jinliang Li, Xuan Zeng, Peibo Li
Summary: In this study, Hypericum japonicum extract (HJ) was found to have antiviral effects against PEDV. In vitro experiments showed that HJ could directly inactivate PEDV and inhibit its proliferation at non-cytotoxic concentrations. In vivo experiments demonstrated that HJ could reduce viral titers in the intestines of infected piglets and improve their intestinal pathological, suggesting that HJ has the potential to be developed as an anti-PEDV drug.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiao-Han Li, Ming-Hong Sun, Wen-Jie Jiang, Dongjie Zhou, Song-Hee Lee, Geun Heo, Zhi Chen, Xiang-Shun Cui
Summary: ZSCAN4 is crucial for porcine embryo development by regulating telomere length through controlling DNMT1 expression and transcriptional activity. Depletion of ZSCAN4 leads to DNA damage, shortened telomere, and developmental defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)