Article
Biochemistry & Molecular Biology
Zdenek Fisar, Jana Hroudova, Martina Zverova, Roman Jirak, Jiri Raboch, Eva Kitzlerova
Summary: Mitochondrial dysfunction is a crucial cellular characteristic of aging and neurodegeneration, and platelets serve as a useful model to study its systemic manifestations. The age dependence of mitochondrial parameters was evaluated, and the impact of cognitive impairment was examined. The study found that mitochondrial enzyme activity and parameters of mitochondrial respiration showed a significant negative correlation with age, but there was no significant difference in age-related changes between individuals with and without cognitive impairment. These findings suggest that measuring mitochondrial respiration in intact platelets could be a potential means to assess age-related mitochondrial dysfunction.
Article
Immunology
Pekka Hurme, Miisa Komulainen, Marleena Tulkki, Annamari Leino, Beate Ruckert, Riitta Turunen, Tytti Vuorinen, Mubeccel Akdis, Cezmi A. Akdis, Tuomas Jartti
Summary: The cytokine profiles of Rhinovirus (RV) and respiratory syncytial virus (RSV)-induced first wheezing episode differ, with higher cytokine expression and longer hospitalization time in the RSV group. In the RV group, increased expression of I-309 (CCL1) and TARC is associated with fewer relapses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Harshada Ketkar, Maha Alqahtani, Samantha Tang, Sreema Puthiya Parambath, Chandra Shekhar Bakshi, Sudhir Jain
Summary: Aging and hypertension can increase susceptibility to infection-related illnesses. The renin-angiotensin system and genetic variations in the AT1R gene play a role in blood pressure regulation and the development of hypertension. In a study using transgenic mice, the haplotype-I variant of AT1R gene was associated with increased susceptibility and lethality in a bacterial infection model, especially in aged animals.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xin-Yao Li, Mei Liu, Ya-Jing Fu, Yong -Jun Jiang, Zi-Ning Zhang
Summary: This meta-analysis investigates the relationship between changes in cytokine levels after treatment and the prognosis of patients with sepsis. The study finds that reduced levels of IL-6 and TNF-alpha after treatment are associated with better prognosis and survival in sepsis patients.
Article
Multidisciplinary Sciences
Petra Kameritsch, Miriam Singer, Christoph Nuernbergk, Natalia Rios, Anibal M. Reyes, Kjestine Schmidt, Julian Kirsch, Holger Schneider, Susanna Mueller, Kristin Pogoda, Ruicen Cui, Thomas Kirchner, Cor de Wit, Baerbel Lange-Sperandio, Ulrich Pohl, Marcus Conrad, Rafael Radi, Heike Beck
Summary: The study reveals the crucial role of endothelial TrxR2 in regulating the steady-state concentration of peroxynitrite and maintaining vascular integrity. Deletion of the TrxR2 gene leads to increased vascular stiffness, hypertrophy, and renal abnormalities. Loss of TrxR2 results in elevated peroxynitrite levels in both vascular endothelial cells and vessels, indicating a potential disruption of vascular homeostasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Medicine, General & Internal
Carla Sala, Marta Rescaldani, Elisa Gherbesi, Gianni Bolla, Cesare Cuspidi, Massimiliano Ruscica, Stefano Carugo
Summary: The present study found that chronic aldosterone excess impairs the platelet nitric oxide/cGMP pathway in humans, possibly due to potassium depletion caused by aldosterone.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Critical Care Medicine
Wei Huang, Xiaoting Wang, Hongmin Zhang, Guangjian Wang, Fang Xie, Dawei Liu
Summary: The study investigated the feasibility and value of using mitochondrial quality control-related proteins as biomarkers in septic patients. The levels of these proteins were significantly correlated with organ dysfunction and reflected disease progression and severity, providing important predictive and guidance value for outcome and treatment.
Article
Immunology
Feng Wu, Yan-Ting Zhang, Fei Teng, Hui-Hua Li, Shu-Bin Guo
Summary: S100a8/a9 plays a critical role in mediating sepsis-induced cardiomyopathy (SIC) by activating the TLR4-ERK1/2-Drp1-dependent pathway, leading to mitochondrial fission and dysfunction. Blocking S100a8/a9 may serve as a promising therapeutic strategy to prevent SIC in sepsis patients.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Anesthesiology
Mathieu Blot, Marine Jacquier, Laure-Anne Pauchard, Chloe Rebaud, Charline Marlin, Camille Hamelle, Amandine Bataille, Delphine Croisier, Charles Thomas, Antoine Jalil, Helene Mirfendereski, Lionel Piroth, Pascal Chavanet, Danielle Bensoussan, Caroline Laroye, Loic Reppel, Pierre-Emmanuel Charles
Summary: This study investigates the immune and mitochondrial dysfunction caused by mechanical ventilation and pneumococcal pneumonia, as well as the therapeutic effects of mesenchymal stem cells and antibiotics. The results demonstrate that high-pressure mechanical ventilation reduces survival rates in infected animals, while the combination of mesenchymal stem cells and ceftaroline improves the treatment outcome.
Article
Biochemistry & Molecular Biology
Hui-Qi Qu, James Snyder, John Connolly, Joseph Glessner, Charlly Kao, Patrick Sleiman, Hakon Hakonarson
Summary: This study investigates the associations of cytokines LIGHT and IL-18 with ARDS, AHRF, or AKI caused by non-COVID-19 viral or bacterial sepsis. The results show increased levels of LIGHT in bacterial sepsis and sepsis from viral infections, while IL-18 levels vary greatly and are consistently associated with severity scores, mortality, and AKI.
Article
Biochemistry & Molecular Biology
Ivana A. Rukavina-Mikusic, Micaela Rey, Manuela Martinefski, Valeria Tripodi, Laura B. Valdez
Summary: This study aimed to investigate the temporal evolution of cardiac mitochondrial dysfunction in a type 1 diabetes model, finding that dysfunction occurred in the early stage of diabetic rats, similar to 7 days of hyperglycemia. Early subcellular signals of cardiac mitochondrial dysfunction include complex I, H2O2, and NO, with NO and H2O2 being located upstream de novo synthesis of mitochondria.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Infectious Diseases
Danni He, Ning Li, Xiuxiu Lu, Wei Li, Yuanmei Chen, Zhongyuan Sun, Lipeng Zhang, Linying Guo, Xiaodai Cui, Guowei Song, Wenquan Niu, Qi Zhang
Summary: This study found that low serum CoQ10 and complex I + III levels are closely associated with the risk and mortality rate of pediatric sepsis in Chinese children.
BMC INFECTIOUS DISEASES
(2022)
Article
Pharmacology & Pharmacy
Mary J. Fronrath, Laura Hencken, Carolyn R. Martz, Bryan Kelly, Zachary R. Smith
Summary: In the management of septic patients, those with pulmonary hypertension (PH) are more likely to require escalation of respiratory support within 72 hours compared to those without PH, despite receiving lower fluid resuscitation volumes.
Review
Infectious Diseases
Manas Kotepui, Thitinat Duangchan, Aongart Mahittikorn, Chusana Mekhora, Nsoh Godwin Anabire, Kwuntida Uthaisar Kotepui
Summary: This systematic review examined the variations in interleukin-5 (IL-5) levels in patients with malaria. The results showed heterogeneity in IL-5 levels between different severities of malaria and uninfected controls. Most studies found no significant difference in IL-5 levels between malaria cases and uninfected controls.
Article
Biochemistry & Molecular Biology
Miklos Lipcsey, Maria Bergquist, Rebecca Siren, Anders Larsson, Fredrik Huss, Jay Pravda, Mia Furebring, Jan Sjolin, Helena Janols
Summary: Hydrogen peroxide (H2O2) and oxidative stress may be responsible for the inflammatory response and increased vascular permeability in sepsis, septic shock, and burn injury patients. The concentration of H2O2 in urine is lower in patients with severe infections, but higher in non-survivors. Therefore, increased urinary H2O2 levels are associated with mortality in sepsis, septic shock, and burn injury patients.
Article
Biochemistry & Molecular Biology
Alex Junker, Jennifer Wang, Gilles Gouspillou, Johannes K. Ehinger, Eskil Elmer, Fredrik Sjovall, Kelsey H. Fisher-Wellman, P. Darrell Neufer, Anthony J. A. Molina, Luigi Ferrucci, Martin Picard
Summary: This meta-analysis examined the biological differences in mitochondria between women and men and found that only a few measures exhibit binary sex differences. The sex differences showed weak or no correlation with age and body mass index. This suggests that there is a wide variability of findings in the literature concerning possible binary sex differences in mitochondrial biology.
Article
Biochemistry & Molecular Biology
Takayuki Kobayashi, Hiroyuki Uchino, Eskil Elmer, Yukihiko Ogihara, Hidetoshi Fujita, Shusuke Sekine, Yusuke Ishida, Iwao Saiki, Shoichiro Shibata, Aya Kawachi
Summary: In this study, a SAE model was established using CypD KO mice, and metabolomic analysis showed that the GSH levels were maintained in the KO animals following CLP. This suggests that CypD may play a role in the pathogenesis of SAE by inhibiting MPTP induction and protecting mitochondrial and cellular function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Anesthesiology
Edgars Grins, Per Ederoth, Henrik Bjursten, Alain Dardashti, Bjorn Bronden, Carsten Metzsch, Andre Erdling, Shahab Nozohoor, Arash Mokhtari, Magnus J. Hansson, Eskil Elmer, Lars Algotsson, Nabin Manandhar Shrestha, Stefan Jovinge
Summary: The study aimed to investigate the effects of preoperative cyclosporine treatment on cytokine production and factors associated with postoperative kidney impairment. The results showed that surgery triggers elevation of cytokine levels, but this activation was not impacted by preoperative cyclosporine treatment.
JOURNAL OF CARDIOTHORACIC AND VASCULAR ANESTHESIA
(2022)
Article
Microbiology
Natalija Karabasevic, Jason A. Roberts, Luke Stronach, Saiyuri Naicker, Steven C. Wallis, Fredrik Sjovall, Fekade Sime
Summary: The combination therapy of meropenem and ciprofloxacin may provide significantly enhanced bacterial killing and resistance suppression against Pseudomonas aeruginosa.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Cell Biology
Sarah Piel, Joanna Janowska, J. Laurenson Ward, Meagan J. McManus, Danielle Aronowitz, Piotr K. Janowski, Jonathan Starr, Jordan N. Hook, Marco M. Hefti, Carly L. Clayman, Eskil Elmer, Magnus J. Hansson, David H. Jang, Michael Karlsson, Johannes K. Ehinger, Todd J. Kilbaugh
Summary: Sodium fluoroacetate is a metabolic poison that inhibits the TCA cycle, posing a significant threat to society. This study investigates succinate prodrugs as a potential treatment for acute sodium fluoroacetate intoxication. The results show that succinate prodrugs can alleviate mitochondrial dysfunction and metabolic crisis caused by sodium fluoroacetate.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Oncology
Sonia Simon Serrano, Michele Tavecchio, Josef Mallik, Alvar Gronberg, Eskil Elmer, Chamseddine Kifagi, Philippe Gallay, Magnus Joakim Hansson, Ramin Massoumi
Summary: Cyclophilins, which are overexpressed in hepatocellular carcinoma (HCC), play a key role in cancer progression and mediating chemoresistance. The novel cyclophilin inhibitor NV651 can reduce cell division and DNA repair in HCC cells. Combination treatment of NV651 and cisplatin significantly increases cancer cell death compared to cisplatin alone.
Article
Biochemistry & Molecular Biology
Andreas Nord, Imen Chamkha, Eskil Elmer
Summary: Understanding mitochondrial biology and pathology is crucial for understanding animal evolution. However, current mitochondrial measurement methods are often invasive or terminal, which is challenging for wild models and longitudinal studies. To overcome this limitation, researchers have utilized mitochondria in non-mammal vertebrates' red blood cells for minimally invasive measurement. This study developed a protocol to measure mitochondrial respiration in bird whole blood and found that whole blood measurement provided faster, more reproducible, and more biologically relevant assessment of mitochondrial respiration compared to isolated blood cell measurement. The authors recommend using whole blood approach for future studies unless specific circumstances require isolated blood cell use.
Review
Biochemistry & Molecular Biology
Alina M. Betiu, Lavinia Noveanu, Iasmina M. Hancu, Ana Lascu, Lucian Petrescu, Christoph Maack, Eskil Elmer, Danina M. Muntean
Summary: Mitochondria play a crucial role in the homeostasis of the cardiovascular system, but mitochondrial dysfunction is recognized as a central pathomechanism of cardiovascular diseases. Current cardiovascular medications can have both beneficial and deleterious effects on mitochondria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Sarah Piel, Joanna I. Janowska, J. Laurenson Ward, Meagan J. McManus, Joshua S. Jose, Jonathan Starr, Malkah Sheldon, Carly L. Clayman, Eskil Elmer, Magnus J. Hansson, David H. Jang, Michael Karlsson, Johannes K. Ehinger, Todd J. Kilbaugh
Summary: Pesticides, particularly organophosphates (OPs), cause a significant number of acute poisonings and deaths worldwide annually. Standard treatments can alleviate symptoms, but survivors often experience neurological sequelae. Thus, there is a critical need for the development of adjunctive therapies with new targets. Mitochondrial dysfunction may contribute to the overall toxicity of pesticides.
SCIENTIFIC REPORTS
(2022)
Review
Anesthesiology
Marie Warrer Munch, Anders Granholm, Andreas Bender Jonsson, Fredrik Sjovall, Marie Helleberg, Frederik Botius Hertz, Jakob Steen Andersen, Morten Steensen, Michael Patrick Achiam, Anders Perner, Morten Hylander Moller
Summary: This study aimed to compare the desirable and undesirable effects of piperacillin/tazobactam versus carbapenems in patients with severe bacterial infections. The results suggest that piperacillin/tazobactam may be associated with less favorable outcomes in patients with severe bacterial infections compared to carbapenems.
ACTA ANAESTHESIOLOGICA SCANDINAVICA
(2023)
Article
Biochemistry & Molecular Biology
Carmen C. Garcia-Diaz, Imen Chamkha, Eskil Elmer, Andreas Nord
Summary: Small birds that winter at high latitudes incur large energy costs for thermoregulation during cold nights due to their limited capacity for hypothermia. A study on wild great tits showed that mitochondrial respiration was not affected by night temperatures, but hypothermia was associated with a reduction of leak respiration and an increase in ATP production. This study sheds new light on how small birds survive in harsh winter conditions.
Article
Medicine, Research & Experimental
Ola Borgquist, Leila Naddi, Gracijela Bozovic, Matthias Hellberg, Martin Annborn, Fredrik Sjovall, Maria Adrian, Eva Hettinger, Pia Sjoberg, Thomas Kander
Summary: This study aims to compare the incidence of post-catheterisation central vein stenosis between the right subclavian and the right internal jugular routes for temporary central dialysis catheters (tCDC). The study will randomise patients to either subclavian or internal jugular vein catheterisation and evaluate different outcomes such as patients' discomfort and pain, dysfunction of the tCDC, catheterisation success rate, and mechanical complications. The accuracy of ultrasound examination in detecting central vein stenosis will also be evaluated.
Article
Medicine, General & Internal
Anja Linden, Jane Fisher, Gisela Lilja, Markus Harboe Olsen, Fredrik Sjovall, Marten Jungner, Martin Spangfors, Line Samuelsson, Jonatan Oras, Adam Linder, Johan Unden, T. Kander, Miklos Lipcsey, Niklas Nielsen, Janus C. Jakobsen, Peter Bentzer
Summary: This trial aims to test whether a protocol targeting non-resuscitation fluids can reduce fluid administration compared to usual care. The study design is a multicentre, randomised, feasibility trial, with adult ICU patients with septic shock as participants. The results will be submitted for publication in a peer-reviewed journal.
Review
Clinical Neurology
Magnus J. Hansson, Eskil Elmer
Summary: The complexity and heterogeneity of traumatic brain injury (TBI) have hindered drug development, resulting in an unmet need for effective pharmacological therapies. Cyclosporine, a polypeptide traditionally used for immunosuppression, has shown promising neuroprotective effects and reduction of secondary brain injury in preclinical models of TBI. The cyclosporine formulation NeuroSTAT(®) has also demonstrated positive effects on injury biomarker levels in severe TBI patients. Future research should incorporate advances in biomarkers and neuroimaging to select patients with similar disease pathologies for clinical trials.
