Article
Veterinary Sciences
Lan Wu, Youjun Li, Xueyang Chen, Yuxin Yang, Chun Fang, Yufang Gu, Jing Liu, Xiongyan Liang, Yuying Yang
Summary: This study characterized the genome of the ALV-J strain HB2020 and found it to be a recombinant strain with mutations in the gag gene compared to the prototype strain HPRS-103. Animal experiments showed that HB2020 could cause various neoplastic lesions in chickens.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Sheng Chen, Yiming Yan, Liguo Gao, Shuang Gao, Keyu Feng, Hongxin Li, Xinheng Zhang, Weiguo Chen, Feng Chen, Qingmei Xie
Summary: In this study, proteomic analysis revealed that 53 cellular proteins are incorporated into mature ALV-J virions, with potential functions in the viral life cycle and tumorigenesis. These findings provide new insights into the interaction between ALV-J and its host, and shed light on the cellular requirements for ALV-J infection.
VETERINARY MICROBIOLOGY
(2023)
Article
Microbiology
Liqin Liao, Zhiqiang Wu, Weiguo Chen, Huanmin Zhang, Aijun Li, Yiming Yan, Zi Xie, Hongxin Li, Wencheng Lin, Jingyun Ma, Xinheng Zhang, Qingmei Xie
Summary: Control of ALV-J in chicken breed is a serious issue that needs more attention. The elimination of vertical transmission of ALV-J is not yet well understood, and effective vaccines or drugs have not been developed. This study found that anti-CD81 antibody can inhibit viral infection and prevent vertical transmission of ALV-J in an animal model.
VETERINARY MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Jun Zhang, Li Ma, Tuofan Li, Luyuan Li, Qiuqi Kan, Xiaohui Yao, Quan Xie, Zhimin Wan, Hongxia Shao, Aijian Qin, Jianqiang Ye
Summary: The study revealed that co-infection of CIAV and ALV-J caused significant body weight loss in infected chickens, with CIAV increasing the viral load of ALV-J in tissues. Furthermore, both CIAV and ALV-J significantly inhibited the humoral immunity response to specific viruses in infected chickens.
Article
Biotechnology & Applied Microbiology
Jing Liu, Keli Gao, Dingwei Li, Yang Zeng, Xueyang Chen, Xiongyan Liang, Chun Fang, Yufang Gu, Chunfeng Wang, Yuying Yang
Summary: This study demonstrated that the recombinant invasive Lactobacillus plantarum NC8 expressing Gp85 protein of ALV-J could enhance the immune responses in chickens, leading to increased protection against ALV-J infection. This provides a novel method for exploring live vaccines against ALV-J.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Virology
Liqin Liao, Weiguo Chen, Xiangyu Zhang, Huanmin Zhang, Aijun Li, Yiming Yan, Zi Xie, Hongxing Li, Wencheng Lin, Jingyun Ma, Xinheng Zhang, Qingmei Xie
Summary: This study discovered a novel means of vertical transmission for ALV-J by utilizing host semen extracellular vesicles, and demonstrated the feasibility of this transmission method.
Article
Microbiology
Kunmei Yang, Jianhao Yang, Defang Zhou, Mingjun Zhu, Xusheng Du, Jing Zhou, Shenglong Liu, Ziqiang Cheng
Summary: In this study, the molecular mechanisms contributing to Avian leukosis virus subgroup J (ALV-J) infection were elucidated. It was found that ALV-J hijacked MIF through its p10 and p27 subunit proteins to facilitate infection. MIF overexpression and knockdown experiments as well as treatment with the MIF antagonist ISO-1 confirmed that MIF significantly promoted ALV-J internalization and replication. Additionally, the interaction between the subunit proteins p10 and p27 of Gag and MIF in the cytoplasm was observed. These findings provide insights into the roles of p10/p27 and the host factor MIF in ALV-J infection.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Ning Cui, Xuezhi Cui, Qinghua Huang, Shaohua Yang, Shuai Su, Chuantian Xu, Jianhe Li, Wenfeng Li, Chao Li
Summary: The study isolated an ALV-J strain LH20180301 from broiler breeder chickens, exhibiting new genomic characters in terms of pathogenicity and oncogenicity, and branching with locally prevalent ALV-J strains in yellow chickens in South China.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Virology
Dandan Qiao, Qian He, Xiaowei Cheng, Yongxiu Yao, Venugopal Nair, Hongxia Shao, Aijian Qin, Kun Qian
Summary: This study investigated the role of the Wnt/beta-catenin signaling pathway during avian leukosis virus subgroup J (ALV-J) infection. The results showed that activation of this pathway increased viral replication, while inhibition significantly reduced virus titer and gene expression.
Article
Agriculture, Dairy & Animal Science
X. H. Wu, Z. Q. Yao, Q. Q. Zhao, S. Chen, Z. Z. Hu, Z. Xie, L. Y. Chen, J. Ji, F. Chen, X. H. Zhang, Q. M. Xie
Summary: In this study, a real-time fluorescent RT-RAA method was developed for rapid and specific detection of ALV-J in poultry. The method showed high sensitivity, good repeatability, and could complete the test within a short time. Moreover, there was no cross-reactivity with other avian leukosis virus subgroups. The study results indicated that this method is simple, easy to use, and suitable for clinical and laboratory diagnosis, providing technical support for the prevention and control of ALV-J.
Article
Veterinary Sciences
Xiyao Cui, Xinyue Zhang, Jingwen Xue, Yongxiu Yao, Defang Zhou, Ziqiang Cheng
Summary: This study used TMT-based quantitative proteomics to investigate the molecular mechanism of synergistic replication of avian leukosis virus subgroup J (ALV-J) and reticuloendotheliosis virus (REV). The results showed that integrins may be key regulators in the mechanism of synergistic infection of REV and ALV-J, providing more insight into the pathogenesis of this synergism at the protein level.
BMC VETERINARY RESEARCH
(2022)
Article
Virology
Weiguo Chen, Sheng Chen, Yu Nie, Wenxue Li, Hongxin Li, Xinheng Zhang, Feng Chen, Qingmei Xie
Summary: In recent years, superinfections of avian leukosis virus subgroup J (ALV-J) and infectious bursal disease virus (IBDV) have become more frequent in nature, resulting in increased pathogenicity in infected chickens. This study demonstrates an effective candidate model for studying the outcomes of superinfections with ALV-J and IBDV. The results show that superinfections lead to severe immunosuppression and enhanced viral replication, which contribute to the increased pathogenicity.
Article
Virology
Jingwen Xue, Defang Zhou, Jing Zhou, Xusheng Du, Xinyue Zhang, Xiaoyang Liu, Longying Ding, Ziqiang Cheng
Summary: Co-infection of avian leukosis virus subgroup J (ALV-J) and reticuloendotheliosis virus (REV) in poultry flocks induces synergistic replication, which worsens immunosuppression, growth inhibition, and increased mortality. MicroRNA-155 (miR-155) targets a dual pathway, PRKCI-MAPK8 and TIMP3-MMP2, which interacts with the U3 region of ALV-J and REV to promote synergistic replication between ALV-J and REV.
JOURNAL OF VIROLOGY
(2023)
Article
Veterinary Sciences
Moru Xu, Fusen Hang, Kun Qian, Hongxia Shao, Jianqiang Ye, Aijian Qin
Summary: This report describes a disease characterized by hepatomegaly and splenomegaly in chickens that is closely associated with a novel strain of ALV-J. Other viruses were ruled out as the cause of the disease, highlighting the importance of an ALV-J eradication program.
BMC VETERINARY RESEARCH
(2022)
Article
Virology
Qiaomu Deng, Min Li, Chengwei He, Qiaoe Lu, Yanli Gao, Qiuhong Li, Mengya Shi, Peikun Wang, Ping Wei
Summary: Avian leukosis virus subgroup J (ALV-J) has caused significant economic losses in China by infecting various birds. In-depth analysis of the genetic diversity of ALV-J revealed that current Chinese strains are mainly in Clade 1.3, while Chinese and Egyptian chicken flocks are encountering emerging Clade 2 viruses. The proposed unified classification system for ALV-J will facilitate future studies on epidemiology, genetic evolution, and sequence comparison worldwide.
