Article
Immunology
Christian Schwarz, Yasemin Toere, Vanessa Hoesker, Sabine Ameling, Katja Gruen, Uwe Voelker, P. Christian Schulze, Marcus Franz, Cornelius Faber, Frieder Schaumburg, Silke Niemann, Verena Hoerr
Summary: The study revealed that the virulence profiles and cytotoxicity of S. aureus isolates in vitro were not accurate predictors of the severity of infective endocarditis, but there were differences in the activation and inhibition of pathways connected to the extracellular matrix and inflammatory response. Therefore, a comprehensive understanding of host-pathogen interactions and immune pathways is necessary to evaluate the pathogenic capacity of bacteria.
Article
Biochemistry & Molecular Biology
Svetlana N. Pleskova, Alexander S. Erofeev, Alexander N. Vaneev, Petr V. Gorelkin, Sergey Z. Bobyk, Vasilii S. Kolmogorov, Nikolay A. Bezrukov, Ekaterina V. Lazarenko
Summary: The production of reactive oxygen species (ROS) by a single neutrophil in response to bacterial stimulation was measured using an electrochemical amperometric method and showed significant variability. Stimulation with S. aureus resulted in 5.5-fold higher ROS production compared to stimulation with E. coli. Analysis of the neutrophil population using luminol-dependent biochemiluminescence (BCL) showed a seven-fold increase in ROS production and a 13-fold increase in maximum peak value when stimulated with S. aureus compared to E. coli. This study demonstrated functional heterogeneity within the neutrophil population but consistent specificity in cellular response to different pathogens at both individual cell and population levels.
Article
Immunology
Zhiguo Gong, Jing Zhang, Shuangyi Zhang, Jinshan Cao, Yunhe Fu, Xiaoyu Hu, Jiamin Zhao, Baichen Gu, Qianru Li, Kai Zhang, Peipei Ren, Bo Liu, Wei Mao
Summary: Staphylococcus aureus infection can induce host immune responses, and TLR2, TLR4, and NLRP3 play important roles in regulating the inflammatory response. However, their roles may vary at different timepoints.
MICROBIAL PATHOGENESIS
(2022)
Article
Genetics & Heredity
Alessandra Lima, Ana Carolina Barbosa Caetano, Raquel Hurtado Castillo, Roselane Gonsalves dos Santos, Diego Lucas Neres Rodrigues, Thiago de Jesus Sousa, Rodrigo Bentes Kato, Marcus Vinicius Canario Viana, Anne Cybelle Pinto Gomide, Flavia Figueira Aburjaile, Sandeep Tiwari, Arun Jaiswal, Alfonso Gala-Garcia, Nubia Seyffert, Thiago Luiz de Paula Castro, Bertram Brenig, Mateus Matiuzzi da Costa, Elaine Maria Seles Dorneles, Yves Le Loir, Vasco Azevedo
Summary: This study performed comparative genomic analyses of 73 Staphylococcus aureus genomes from different hosts, including 12 genomes isolated from sheep. The study revealed the genomic specialization and the role of specific genes in establishing infection in ovine mastitis-associated S. aureus.
Article
Biology
Marc Schulte, Katharina Olschewski, Michael Hensel
Summary: The study demonstrates that non-replicating Salmonella enterica serovar Typhimurium persisters, which are tolerant to antibiotics, can sense their environment and respond to stressors. This suggests that stress sensing and response of persisters may be targeted as an antimicrobial strategy.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ming-Ji Wei, Zhen-Nan Wang, Yan Yang, Shu-Jiu Zhang, He Tang, Hui Li, Chong-Liang Bi
Summary: The study found that selenium can significantly reduce the inflammation induced by S. aureus by suppressing the activation of the TLR2 signaling pathway and NLRP3 inflammasome.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Ecology
Alice K. E. Ekroth, Michael Gerth, Emily J. Stevens, Suzanne A. Ford, Kayla C. King
Summary: The study found that host genotype and genetic diversity have an impact on pathogen evolution, with different host genotypes leading to variations in pathogen virulence, possibly driven by differences in host metal ion acquisition. Diverse host populations selected for the highest levels of pathogen virulence, but infectivity was constrained.
Article
Gastroenterology & Hepatology
Xiaoxiao Mi, Yu Song, Chaohua Deng, Jian Yan, Zhihui Li, Yingniang Li, Jun Zheng, Wenjun Yang, Ling Gong, Junping Shi
Summary: This study investigates the function and heterogeneity of neutrophils in Wilson's disease using zebrafish, mice, and cellular models. The researchers discover that pharmacologic modulation of N2-neutrophil activity could be explored as an alternative therapeutic approach to improve liver function in Wilson's disease.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Cell Biology
Adrian Kobiela, Lilit Hovhannisyan, Paulina Jurkowska, Jorge Bernardino de la Serna, Aleksandra Bogucka, Milena Deptula, Argho Aninda Paul, Kinga Panek, Ewa Czechowska, Michal Rychlowski, Aleksandra Krolicka, Jacek Zielinski, Susanne Gabrielsson, Michal Pikula, Magdalena Trzeciak, Graham S. Ogg, Danuta Gutowska-Owsiak
Summary: Filaggrin (FLG) protein plays a crucial role in maintaining the skin barrier function, but its excessive accumulation can lead to premature cell death. Researchers have discovered that small extracellular vesicles (sEVs) secreted by keratinocytes may contain filaggrin-related cargo, providing a mechanism for eliminating excess filaggrin. The bacteria Staphylococcus aureus (S. aureus) promotes the packaging and secretion of filaggrin within sEVs, facilitating its removal from the skin and potentially aiding bacterial growth.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Microbiology
Tiep K. Nguyen, Frederic Peyrusson, Wafi Siala, Nhung H. Pham, Hoang A. Nguyen, Paul M. Tulkens, Francoise Van Bambeke
Summary: Staphylococcus aureus biofilms are resistant to antibiotics due to factors such as drug access blockage and presence of dormant bacteria. Clinical isolates produce more biofilm biomass than the reference strain, especially those with high persister fractions. Moxifloxacin is less effective against biofilms from clinical isolates, attributed to lower drug concentration and presence of moxifloxacin-tolerant subpopulations.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Hui Liu, Xing Ji, Heye Wang, Xiang Hou, Haichang Sun, Craig Billington, Lili Zhang, Xiaoye Wang, Ran Wang
Summary: This study investigated the genomic epidemiology of Staphylococcus aureus isolates from raw milk in Jiangsu Province, China. The major lineages identified were CC1-ST1, CC97-ST97, CC398-ST398, CC8-ST630, and CC59-ST59. Methicillin-resistant Staphylococcus aureus (MRSA) lineages and phenotypic resistance were also observed. Isolates that produced biofilms and invaded cells were associated with specific adherence-associated gene patterns and lineages. Additionally, many isolates contained enterotoxin genes, posing a potential risk to human health. This study highlights the importance of monitoring Staphylococcus aureus in the dairy industry.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Immunology
Dominique Missiakas, Volker Winstel
Summary: Host cell death programs are essential for cellular homeostasis, embryonic development, and tissue regeneration. Pathogens like Staphylococcus aureus use cell death pathways to establish infection, manipulating apoptotic, necroptotic, and pyroptotic modes to shape the immune environment conducive to persistence. This exploitation of cell death pathways is a virulence strategy that must be considered alongside immune evasion mechanisms for the development of effective therapeutics and interventions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Mengyao Wang, Yu Qi, Yutao Cao, Xiaosheng Zhang, Yongsheng Wang, Qingyou Liu, Jinlong Zhang, Guangbin Zhou, Yue Ai, Shao Wei, Linli Wang, Guoshi Liu, Zhengxing Lian, Hongbing Han
Summary: Staphylococcus aureus infections pose a potential threat to livestock production and public health. This study has constructed a novel receptor TLR2-4, which activates multiple signaling pathways to enhance autophagy and increase the clearance of S. aureus.
Article
Infectious Diseases
Hongying Zhu, Hong Luo, Qiaoshi Zhong, Xingwei Cao, Shumin Gu, Suqin Peng, Yanping Xiao, Yanhui Chen, Yaping Hang, Xueyao Fang, Shan Zou, Fangyou Yu, Longhua Hu
Summary: This study compared the molecular features of MSSA and MRSA isolates and found differences in sequence types, virulence genes, and antimicrobial resistance. The findings highlight the need to intensify efforts in preventing and treating MSSA infections. Further large-scale comparative epidemiological studies are necessary to understand the evolution and interaction of MSSA and MRSA populations in the future.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Hematology
Sidan Li, Juo-Chin Yao, Karolyn A. Oetjen, Joseph R. Krambs, Jun Xia, Jingzhu Zhang, Amy P. Schmidt, Nichole M. Helton, Robert S. Fulton, Sharon E. Heath, Isaiah R. Turnbull, Gabriel Mbalaviele, Timothy J. Ley, Matthew J. Walter, Daniel C. Link
Summary: This study reveals that perivascular bone marrow dendritic cells (DCs) express Toll-like receptor-1 (TLR1) and TLR2, which can induce expansion and mobilization of hematopoietic stem/progenitor cells (HSPCs). The stimulation of TLR1/2 in DCs results in the secretion of IL-1β and other inflammatory cytokines, which regulates HSPC activity within the hematopoietic niche. The findings suggest that increased DC TLR1/2 signaling may contribute to altered HSPC function in myelodysplastic syndrome.
Article
Developmental Biology
Johanne Johnsen Rakner, Gabriela Brettas Silva, Siv Boon Mundal, Astrid Josefin Thaning, Mattijs Elschot, Jenny Ostrop, Liv Cecilie Vestrheim Thomsen, Line Bjorge, Lobke Marijn Gierman, Ann-Charlotte Iversen
Summary: Inflammation in preeclampsia affects the interaction between maternal immune cells and fetal trophoblasts, with the pattern recognition receptor nucleotide-binding oligomerization domain-containing protein (NOD)1 playing a significant role. It was found that in preeclamptic pregnancies, there is an increase in NOD1 expression density, and strong expression of NOD1 is associated with trophoblast types.
Article
Cell Biology
Jenny Ostrop, Rosalie T. Zwiggelaar, Marianne Terndrup Pedersen, Francois Gerbe, Korbinian Bosl, Havard T. Lindholm, Alberto Diez-Sanchez, Naveen Parmar, Silke Radetzki, Jens Peter von Kries, Philippe Jay, Kim B. Jensen, Cheryl Arrowsmith, Menno J. Oudhoff
Summary: By developing a semi-automated organoid screening method, researchers identified several targets that affect epithelial cell differentiation, including HDACs, EP300/CREBBP, LSD1, and type I PRMTs, which were verified by complementary methods. The study also showed that inhibiting type I PRMTs promotes enhanced epithelial differentiation, blocking adenoma growth while not affecting normal organoid cultures.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Olga Ticha, Peter Slanina, Lukas Moos, Julie Stichova, Marcela Vlkova, Isabelle Bekeredjian-Ding
Summary: TNFR2 expression is associated with tolerogenic immune reactions and suppressor cells, IL-10 secretion is a characteristic of memory B cells, and Breg formation is a specific feature of human memory B cells undergoing terminal differentiation.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Microbiology
Masanori Matsumoto, Seitaro Nakagawa, Lingzhi Zhang, Yuumi Nakamura, Amer E. Villaruz, Michael Otto, Christiane Wolz, Naohiro Inohara, Gabriel Nunez
Summary: The growth and invasion of Staphylococcus aureus in the skin are differently regulated by Agr and PSM alpha, with Agr limiting intracellular killing within neutrophils to promote pathogen expansion in the dermis and subcutaneous tissue.
CELL HOST & MICROBE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Karin Jepsen, Wolfgang Falk, Friederike Brune, Rolf Fimmers, Soren Jepsen, Isabelle Bekeredjian-Ding
Summary: This study found a high prevalence of periodontal pathogens in the subgingival microbiota of German periodontitis patients, with an increasing trend in isolates displaying resistance to some antibiotics over time. However, there was no significant change in susceptibility to amoxicillin and metronidazole.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2021)
Article
Multidisciplinary Sciences
Christof Audretsch, Fabio Gratani, Christiane Wolz, Thomas Dandekar
Summary: This study investigated the regulatory mechanisms of amino acid requirement in Stapylococcus aureus and validated the findings through in silico modeling, showing a high level of correlation between the model predictions and experimental results. The research highlights the significance of stringent response and central regulators like CodY in amino acid metabolism of S. aureus.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Marilena P. Etna, Aurora Signorazzi, Daniela Ricci, Martina Severa, Fabiana Rizzo, Elena Giacomini, Andrea Gaggioli, Isabelle Bekeredjian-Ding, Anke Huckriede, Eliana M. Coccia
Summary: The study showed that inactivated TBEV (I-TBEV) induced differentiation of a sub-population of plasmacytoid dendritic cells (pDC) specialized in type I interferon production in human PBMC. This was critical for B cell differentiation to plasmablasts, highlighting the importance of preserving key viral molecules during vaccine manufacturing process.
Article
Pharmacology & Pharmacy
Helene Kolstad Skovdahl, Shreya Gopalakrishnan, Tarjei Dahl Svendsen, Atle van Beelen Granlund, Ingunn Bakke, Zekarias G. Ginbot, Silje Thorsvik, Arnar Flatberg, Bjornar Sporsheim, Jenny Ostrop, Tom Eirik Mollnes, Arne Kristian Sandvik, Torunn Bruland
Summary: Treatment of inflammatory bowel disease (IBD) is challenging due to the limited efficacy of available drugs, highlighting the need for biomarkers and assays to predict drug effect. The intestinal epithelium plays a crucial role in disease pathogenesis and offers a promising target for IBD treatments. Lowering oxygen concentration can better mimic the in vivo physiological environment of colonic epithelial cells and enhance the translational value of intestinal organoids in drug testing.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Immunology
Olga Ticha, Dido Klemm, Lukas Moos, Isabelle Bekeredjian-Ding
Summary: This study aimed to develop a reliable in vitro assay for characterizing the antigen of tetanus vaccines, with the potential to replace in vivo experiments. Results showed that using PBMC from donor samples to detect TT-specific IgG can reveal specific responses after vaccination.
Article
Biochemistry & Molecular Biology
Siv Boon Mundal, Johanne Johnsen Rakner, Gabriela Brettas Silva, Lobke Marijn Gierman, Marie Austdal, Purusotam Basnet, Mattijs Elschot, Siril Skaret Bakke, Jenny Ostrop, Liv Cecilie Vestrheim Thomsen, Eric Keith Moses, Ganesh Acharya, Line Bjorge, Ann-Charlotte Iversen
Summary: Utero-placental development in pregnancy depends on direct maternal-fetal interaction in the uterine wall decidua. Abnormal uterine vascular remodeling preceding placental oxidative stress and placental dysfunction are associated with preeclampsia and fetal growth restriction (FGR).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Lisa Bleul, Patrice Francois, Christiane Wolz
Summary: This review summarizes the current understanding of signal perception by histidine kinases in Staphylococcus aureus and their role in promoting appropriate gene expression in response to environmental challenges.
Article
Infectious Diseases
Maria Auxiliadora Miranda-Garcia, Marcus Hoffelner, Hagen Stoll, Dorte Ruhaltinger, Klaus Cichutek, Anette Siedler, Isabelle Bekeredjian-Ding
Summary: This study provides a retrospective analysis of supply issues and measures in Germany in the context of European and global vaccine supply constraints. The study found that vaccine supply shortages in Germany were mainly caused by increased demand, changes in vaccine recommendations, and global immunisation program changes. Shortages of a shorter duration could be mitigated using existing storage capacities, while longer shortages often required the use of alternative products and redistribution.
Article
Immunology
Havard T. Lindholm, Naveen Parmar, Claire Drurey, Marta Campillo Poveda, Pia M. Vornewald, Jenny Ostrop, Alberto Diez-Sanchez, Rick M. Maizels, Menno J. Oudhoff
Summary: This study investigated immune responses in the intestinal epithelium using organoid image analysis, transcriptomic analysis, and in vivo infection models. The researchers found that IL-13 and IL-22 induce genes associated with goblet cells, but the resulting phenotypes of goblet cells are different. Additionally, only IL-13-driven goblet cells are associated with classical NOTCH signaling. The study also revealed that IL-13 activates the BMP pathway, which negatively regulates type 2 immunity and tuft cell hyperplasia.
SCIENCE IMMUNOLOGY
(2022)
Article
Virology
Carina Rohmer, Ronja Dobritz, Dilek Tuncbilek-Dere, Esther Lehmann, David Gerlach, Shilpa Elizabeth George, Taeok Bae, Kay Nieselt, Christiane Wolz
Summary: Staphylococcus aureus asymptomatically colonizes the nasal cavity of mammals and can cause life-threatening infections. This study investigates the role of Sa3 phages in the adaptation of S. aureus strains. The genetic makeup of the host strains appears to determine the rate of phage mobilization, which may impact the speed at which certain strains can achieve host adaptation.
Article
Biochemistry & Molecular Biology
Janina Bayer, Janna Becker, Xiao Liu, Lisa Gritsch, Ellen Daiber, Natalya Korn, Filipp Oesterhelt, Martin Fraunholz, Alexander Weber, Christiane Wolz
Summary: The less pathogenic Coagulase-negative Staphylococcal (CoNS) species are efficiently cleared in macrophages, while non-toxic Staphylococcus aureus can survive within them. Mutations in certain genes do not affect bacterial survival in different types of cells. However, the sensitivity to acidification is linked to species differences.
MOLECULAR MICROBIOLOGY
(2023)