Article
Chemistry, Analytical
Juvissan Aguedo, Zuzana Pakanova, Lenka Lorencova, Marek Nemcovic, Peter Kasak, Marek Barath, Pavol Farkas, Jan Tkac
Summary: In this study, MXene was used as a column cartridge for N-glycan enrichment from human samples, with a focus on the analysis of sialic acid linkages. The MXene-based cartridge enriched a higher number of glycans compared to commercial HILIC columns. This study demonstrates the strong potential of MXene as a stationary phase in MS glycomic analysis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Microbiology
Christopher J. Day, Alvin W. Lo, Lauren E. Hartley-Tassell, M. Pilar Argente, Jessica Poole, Nathan P. King, Joe Tiralongo, Michael P. Jennings, Mark A. Schembri
Summary: This study focused on dissecting the receptor specificity of different CU fimbriae in Escherichia coli using a glycan array analysis approach with whole-bacterial-cell surface plasmon resonance. The research revealed new information on fimbrial specificity and provided a rapid and scalable system to define novel adhesin-glycan interactions underlying bacterial colonization and disease. The findings contribute to a better understanding of bacterial adhesion mechanisms and offer a promising strategy to combat antibiotic resistance through blocking adhesion.
Review
Biochemistry & Molecular Biology
Luka Peric, Sonja Vukadin, Ana Petrovic, Lucija Kuna, Nora Puseljic, Renata Sikora, Karla Rozac, Aleksandar Vcev, Martina Smolic
Summary: This review summarizes the important information about glycosylation characteristics in breast cancer cells and discusses how glycoproteins and enzymes involved in glycosylation could serve as more precise biomarkers and therapeutic targets.
Article
Immunology
Hayley Goodson, Rebeca Kawahara, Sayantani Chatterjee, Gabriel Goncalves, Joshua Fehring, Anthony W. Purcell, Nathan P. Croft, Morten Thaysen-Andersen
Summary: Immunopeptidomics study reveals that N-glycan modifications are prevalent in MHC-II immunopeptides, but the complex/hybrid-type N-glycan modifications are rare compared to the cellular glycoproteome.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Yongjing Xie, Leticia Martins Mota, Adam Bergin, Roisin O'Flaherty, Aled Jones, Bethan Morgan, Michael Butler
Summary: This study presents an improved 96-well plate format platform for streamlined glycan profiling, allowing high sensitivity and high-throughput identification and quantification of diverse N-glycans. The technology offers a powerful tool for rapid advancement in glycan analysis for biopharmaceutical development and biomarker discovery in clinical disease diagnosis.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
Peilin Jiang, Wenjing Peng, Jingfu Zhao, Mona Goli, Yifan Huang, Yunxiang Li, Yehia Mechref
Summary: The complexity and heterogeneity of protein glycosylation present challenges in characterization and quantitation. This study introduces a metabolic isotope labeling strategy called GlyProSILC, which can label both the glycan motif and peptide backbone in the same batch of cells. It provides an accurate and effective quantitation approach for glycomics, proteomics, and glycoproteomics in a cell culture system.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Wenjing Ma, Zhuojia Xu, Yuhan Jiang, Jialin Liu, Dandan Xu, Wei Huang, Tiehai Li
Summary: This study synthesized a library of IgG N-glycans covering all possible biantennary and bisected structures by reprogramming biosynthetic assembly lines and deciphered their unique binding specificities with different proteins using glycan microarray technology. This synthetic IgG N-glycan library can be used as standards for N-glycan structure identification in complex biological samples and the microarray data enrich N-glycan glycomics for biomedical applications.
Article
Biochemical Research Methods
Xiaoyong Zhao, Lin Zeng, Jiaqi Wang, Yanna Shi, Bo Zhang, Yaqin Liu, Yuanjiang Pan, Xian Li
Summary: This study provides the first systematic insight into the N-glycosylation of peach fruit during ripening. Mass spectrometry analysis revealed differential expression of 6 out of 24 N-glycans in peach. Comparative N-glycoproteome analysis identified 1464 N-glycosites on 881 N-glycoproteins, with 291 N-glycosites on 237 N-glycoproteins showing differential expression. The enrichment analysis revealed important pathways and dynamic changes in N-glycoproteins during fruit ripening.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Abhilash Nitin Deo, Rahul Thorat, Ajit Chandrakant Dhadve, Abhijit De, Bharat Rekhi, Pritha Ray
Summary: Recurrent metastatic epithelial ovarian cancer (EOC) poses challenges due to treatment limitations, with chemoresistance exacerbating organ-specific metastasis through the IGF1R-alpha 6 integrin-S100A4 molecular network. S100A4 may serve as a potential target for treating recurrent metastatic EOC.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Biochemical Research Methods
Si Ying Lim, Bao Hui Ng, Dhruti Vermulapalli, Hazel Lau, Anna Karen Carrasco Laserna, Xiaoxun Yang, Sock Hwee Tan, Mark Y. Chan, Sam Fong Yau Li
Summary: Bioinformatics and machine learning tools have enabled the integration of data from different omics platforms for novel insights into diseases. A simultaneous dual extraction method has been introduced for efficient and reproducible generation of metabolomic and glycomic profiles. The joint-omics analysis identified mechanistic interactions between metabolites and glycans, revealing the intermodulatory roles of protein-bound carbohydrates or glycoproteins and amino acids in metabolic pathways and the protective role of the glyco-redox network in coronary heart disease.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Stefan Gaunitz, Lars O. Tjernberg, Sophia Schedin-Weiss
Summary: Protein glycosylation plays a crucial role in the development of Alzheimer's disease, with altered glycan profiles observed in the hippocampus and cortex of AD patients. The glycans that differ between controls and AD brain have similar structures with specific components, indicating a potential biomarker for AD. These findings suggest that glycosylation is an important component in the development of AD and warrant further studies.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Roisin O'Flaherty, Adam Simon, Manuela Alonso-Sampedro, Sonia Sanchez-Batan, Carmen Fernandez-Merino, Francisco Gude, Radka Saldova, Arturo Gonzalez-Quintela
Summary: The study evaluates the diagnostic capability of serum N-glycosylation to identify alcohol risk drinking compared to standard markers. Results show significant variations in serum N-glycome associated with risk drinking, with a unique combination of N-glycan peaks providing good discrimination between risk-drinkers and non-risk drinkers for both men and women. Including N-glycan peaks improves the diagnostic accuracy of standard markers, although sensitivity remains relatively low.
Article
Biochemical Research Methods
Cheng Li, Wei Dan, Pengfei Li, Miaomiao Xin, Rongxia Lan, Bojing Zhu, Zexuan Chen, Wenbo Dong, Liuyi Dang, Xinwen Zhang, Shisheng Sun
Summary: In this study, a glycoproteomic analysis of human semen during the liquefaction process was conducted. The analysis revealed that 25 intact glycopeptides from 10 glycoproteins were significantly changed, with half of them being modified with sialylated glycans. These findings provide valuable insights into the role of glycosylation during semen liquefaction.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Medicinal
Audrey Auger, Shin-Yi Yu, Shih-Yun Guu, Agnes Quemener, Gabriel Euller-Nicolas, Hiromune Ando, Marion Desdouits, Francoise S. Le Guyader, Kay-Hooi Khoo, Jacques Le Pendu, Frederic Chirat, Yann Guerardel
Summary: Noroviruses bind to HBGAs on host tissues and oysters can act as vectors for viral infection due to their synthesis of similar glycan epitopes. The methylation patterns of N-glycans in oysters, particularly in N-acetylgalactosamine and fucose residues, can influence the recognition events between oysters and norovirus capsid proteins.
Article
Biochemical Research Methods
Yi Wang, Si Liu, Jiaoyuan Li, Tongxin Yin, Yuanyuan Liu, Qiankun Wang, Xin Liu, Liming Cheng
Summary: In this study, serum N-glycan profiles of NSCLC patients and healthy controls were characterized and compared. A panel of N-glycan biomarkers for NSCLC diagnosis was established and validated using machine learning algorithms. The optimized biomarker panel showed a high diagnostic accuracy in distinguishing NSCLC patients from healthy controls, especially in early-stage patients.
Article
Hematology
Marita Westhrin, Vlado Kovcic, Zejian Zhang, Siv H. Moen, Tonje Marie Vikene Nedal, Albert Bondt, Stephanie Holst, Kristine Misund, Glenn Buene, Anders Sundan, Anders Waage, Tobias S. Slordahl, Manfred Wuhrer, Therese Standal
Article
Cell & Tissue Engineering
Yong-Jian Zhu, Si-Jin Zhang, Xiao-Han Wu, Tian-Yu Lian, Yang-Zhige He, Ze-Jian Zhang, Dan Lu, Kai Sun, Hui-Fang Wang, Zhi-Cheng Jing
STEM CELL RESEARCH
(2020)
Article
Endocrinology & Metabolism
Zejian Zhang, Karli R. Reiding, Jianqiang Wu, Zepeng Li, Xiequn Xu
Summary: This study comprehensively evaluated the plasma N-glycomic changes in patients with TC or BTN for the first time, determining several N-glycan biomarkers with potential for differential diagnosis and risk assessment of lymph node metastasis. These findings enhance the understanding of TC.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Oncology
Zejian Zhang, Jianqiang Wu, Peng Liu, Lin Kang, Xiequn Xu
Summary: Novel biomarkers are urgently needed to distinguish between benign and malignant thyroid nodules and detect thyroid cancer in the early stage. The associations between serum IgG N-glycosylation and thyroid cancer risk have been revealed. Our results suggest that derived trait BN and the classification glyco-panel may serve as candidate biomarkers for further validation, showing accurate performance in discriminating thyroid cancer from benign thyroid nodules and healthy controls.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jing Zhang, Zejian Zhang, Stephanie Holst, Constantin Bloechl, Katarina Madunic, Manfred Wuhrer, Peter Ten Dijke, Tao Zhang
Summary: Transforming growth factor-beta (TGF-beta) plays a key role in pancreatic ductal adenocarcinoma (PDAC) progression, and glycosylation is associated with it. The study found that PDAC cells deficient in the signal transducer of TGF-beta signaling, SMAD4, exhibit glycosylation changes upon TGF-beta stimulation, including elevated N-glycome aberrations and alterations in O-glycosylation and glycosphingolipid glycosylation profiles.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Allergy
Zejian Zhang, Xue Wang, Jianqing Gu, Jianqiang Wu, Yang Cao, Yingyang Xu, Lisha Li, Kai Guan, Peng Liu, Jia Yin, Yuxiang Zhi, Shuyang Zhang
Summary: This study analyzed the plasma N-glycome of HAE patients and identified abnormal glycan traits compared to healthy controls. Classification models based on dysregulated glycan traits could effectively differentiate between HAE and healthy controls. These findings may have potential implications for predicting disease severity and guiding treatment in clinical practice.
CLINICAL AND TRANSLATIONAL ALLERGY
(2021)
Article
Endocrinology & Metabolism
Zhen Cao, Zejian Zhang, Rui Liu, Mengwei Wu, Zepeng Li, Xiequn Xu, Ziwen Liu
Summary: This study explores novel serum biomarkers for monitoring and predicting recurrence in papillary thyroid cancer (PTC) after thyroidectomy using N-glycomics. The study identifies several candidate serum N-glycan biomarkers, including linkage-specific sialylation, which have potential in predicting recurrence and monitoring the response to initial surgical resection therapy.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shuyu Zhang, Haidan Sun, Zejian Zhang, Menglin Li, Zhengguang Guo, Wenling Ye, Guangyan Cai, Wei Sun, Mingxi Li
Summary: This study demonstrated the potential diagnostic value of O-glycoforms in the hinge region of plasma IgA1 for IgA nephropathy. The levels of GalNAc and galactose were significantly different in IgA nephropathy patients compared to controls, and combining these values improved the diagnostic performance. A panel containing GalNAc, galactose, and circulating IgA showed excellent diagnostic potential.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Hui-fang Wang, Yi-xuan Wang, Yu-ping Zhou, Yun-peng Wei, Yi Yan, Ze-jian Zhang, Zhi-cheng Jing
Summary: O-GlcNAcylation is a protein post-translational modification controlled by O-GlcNAc transferase (OGT) and protein O-GlcNAcase (OGA), and is involved in the development of cardiovascular disease (CVD). Changes in O-GlcNAcylation levels can disrupt metabolic balance and affect cardiovascular function, but its role in CVD is still controversial. This review focuses on the interactions between protein O-GlcNAcylation and CVD and discusses potential molecular mechanisms for the treatment of cardiovascular events.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Oncology
Zhen Cao, Zejian Zhang, Xiaoyue Tang, Rui Liu, Mengwei Wu, Jianqiang Wu, Ziwen Liu
Summary: This study investigated the molecular mechanisms underlying lymph node metastasis (LNM) and its sex disparities in papillary thyroid microcarcinoma (PTMC) using a proteomics approach. The results showed that LNM in PTMC was associated with mitochondrial dysfunction and activation of oxidative phosphorylation and PI3K/AKT signaling pathways. The study also found distinct proteomics profiles between male and female PTMC patients with LNM, with elevated levels of extracellular matrix-associated proteins in male patients. Furthermore, certain proteins were identified to be downregulated and correlated with tumor metastasis, while others were upregulated and correlated with sex disparities and poor prognosis. These findings enhance our understanding of the functional networks and signaling pathways related to PTMC with LNM.
FRONTIERS IN ONCOLOGY
(2022)
Article
Peripheral Vascular Disease
Ze-Jian Zhang, Hui-Fang Wang, Tian-Yu Lian, Yu-Ping Zhou, Xi-Qi Xu, Fan Guo, Yun-Peng Wei, Jing-Yi Li, Kai Sun, Chao Liu, Lu-Rong Pan, Ming Ren, Lei Nie, Hai-Long Dai, Zhi-Cheng Jing
Summary: This study reveals the comprehensive signature of the IgG N-glycome and the regulating genes in a proinflammatory phenotype in CTEPH. Plasma IgG galactosylation may be used for evaluating the inflammatory state in patients with CTEPH, but further validation is needed. This study improves our understanding of the mechanisms underlying CTEPH inflammation from the perspective of glycomics.
Article
Endocrinology & Metabolism
Zejian Zhang, Zhen Cao, Jinhui Wang, Zepeng Li, Tao Wang, Yang Xiang
Summary: This study investigated the serum N-glycome patterns of endometrial cancer (EC) patients and found distinctive differences compared with healthy controls. Abnormal high-mannose and hybrid-type N-glycans, fucosylation, galactosylation, and linkage-specific sialylation were observed in EC patients. A glycan panel constructed with four discriminative traits accurately identified EC with high accuracy.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ming Cui, Ya Hu, Zejian Zhang, Tianqi Chen, Menghua Dai, Qiang Xu, Junchao Guo, Taiping Zhang, Quan Liao, Jun Yu, Yupei Zhao
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Oncology
Zejian Zhang, Zhen Cao, Rui Liu, Zepeng Li, Jianqiang Wu, Xiaoli Liu, Mengwei Wu, Xiequn Xu, Ziwen Liu
Summary: This study characterized the N-glycome of papillary thyroid microcarcinoma (PTMC), and established nomograms for the diagnosis of PTMC and the prediction of lymph node metastasis (LNM). The study found significant differences in serum N-glycome between PTMC and healthy controls, as well as associations between certain N-glycan traits and PTMC and LNM. Nomograms based on these traits showed good performance in the identification of PTMC and the prediction of LNM.
Meeting Abstract
Endocrinology & Metabolism
Marita Westhrin, Vlado Kovcic, Anders Sundan, Albert Bondt, Stephanie Holst, Zejian Zhang, Tobias Slordahl, Anders Waage, Manfred Wuhrer, Therese Standal
JOURNAL OF BONE AND MINERAL RESEARCH
(2019)
