Article
Infectious Diseases
Raquel Garcia-Fierro, Antoine Drapeau, Melody Dazas, Estelle Saras, Carla Rodrigues, Sylvain Brisse, Jean-Yves Madec, Marisa Haenni
Summary: This study performed comparative phylogenomics on a large collection of multidrug-resistant K. pneumoniae recovered from companion animals in France. The results showed the transmission of K. pneumoniae clones between animals and humans, suggesting the circulation of clones at the country level.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Ricardo Oliveira, Joana Castro, Sonia Silva, Hugo Oliveira, Maria Jose Saavedra, Nuno Filipe Azevedo, Carina Almeida
Summary: This study identified a diverse pool of beta-lactam resistance genetic determinants in 102 multi-drug resistant K. pneumoniae isolates from patients in northern Portugal, leading to high levels of resistance to most beta-lactam antibiotics. The co-occurrence of multiple beta-lactam resistance genes in the majority of isolates serves as a serious warning of the problem of antimicrobial resistance.
Article
Infectious Diseases
Se Ra Shin, Seong Mi Noh, Woo Kyung Jung, Sook Shin, Young Kyung Park, Dong Chan Moon, Suk-Kyung Lim, Yong Ho Park, Kun Taek Park
Summary: The study conducted in South Korea between 2017 and 2019 identified various ESC-resistant bacterial isolates in companion animals, with predominant species being E. coli, Klebsiella, and others. These isolates displayed diversity in sequence types and resistance genes.
Article
Infectious Diseases
Zhiwei Zheng, Lianwei Ye, Ruichao Li, Sheng Chen
Summary: This study investigated the prevalence and genetic contexts of the bla(CTX-M-14) gene in foodborne Vibrio isolates in China. The results showed that this gene was widely distributed in Vibrio species and could be transferred horizontally between strains, contributing to the rapid increase in prevalence of bla(CTX-M-14)-bearing bacterial pathogens worldwide.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Fang-Ling Liu, Nan-Ling Kuan, Kuang-Sheng Yeh
Summary: The study found Escherichia coli in dogs and cats with resistance to major antibiotics, some of which were highly virulent. ESBL and pAmpC genes, as well as corresponding pathogenic phylogroups and sequence types, were identified. Imipenem was effective against all ESBL/pAmpC-producing E. coli, but caution is needed in its use in companion animals.
Article
Infectious Diseases
Suraj Shukla, Siddhi Desai, Ashutosh Bagchi, Pushpendra Singh, Madhvi Joshi, Chaitanya Joshi, Jyoti Patankar, Geeti Maheshwari, Ekadashi Rajni, Manali Shah, Devarshi Gajjar
Summary: The prevalence of beta-lactamases, especially carbapenemases, in Klebsiella pneumoniae isolates from India was evaluated in this study. The most prevalent sequence types (ST) among Indian isolates were ST231, ST147, ST2096, and ST14. The gene bla(ampH) was found to be the most prevalent, followed by bla(CTX-M-15) and bla(TEM-1). The study also identified ST16 and ST14 as potential high-risk clones in India, capable of producing both carbapenemases and ESBL genes.
Article
Immunology
Jun Sakai, Shigefumi Maesaki
Summary: This study aimed to distinguish ESBL-producing K. quasipneumoniae (ESBL-KQ) and ESBL-producing K. variicola (ESBL-KV) from ESBL-producing K. pneumoniae (ESBL-KP) in terms of frequency, genomic characteristics, and antimicrobial susceptibility patterns. The study found that ESBL-KQ and ESBL-KP had different gene profiles, and the prevalence of antimicrobial resistance via AmpC and fluoroquinolone-resistance genes increased in ESBL-KQ.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Guoye Liu, Huimin Qian, Jingwen Lv, Benshun Tian, Changjun Bao, Hong Yan, Bing Gu
Summary: The study revealed an increasing trend of antimicrobial resistance in nontyphoidal Salmonella in Jiangsu, China, particularly towards colistin. Plasmid-mediated resistance genes play a significant role in these bacteria, with mcr-1-positive strains predominantly found in children under 7 years old.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Hams M. A. Mohamed, Sulaiman Mohammed Alnasser, Hanan H. Abd-Elhafeez, Meshal Alotaibi, Gaber El-Saber Batiha, Waleed Younis
Summary: Pseudomonas species isolates from chickens showed resistance to most antibiotics, particularly those from the beta-lactamase family. PCR confirmed the correlation between multidrug-resistant isolates and biofilm-related genes, and revealed the potent antibiofilm effect of clove oil against Pseudomonas.
Article
Infectious Diseases
Baptiste Hoellinger, Charlotte Kaeuffer, Pierre Boyer, Nicolas Lefebvre, Yves Hansmann, Amandine Robert, Francois Severac, Alain Gravet, Francois Danion, Yvon Ruch, Axel Ursenbach
Summary: This study compared the use of cefepime with carbapenems for the treatment of third-generation cephalosporin-resistant ABLHE bloodstream infections. The results showed no significant difference in 30-day in-hospital mortality, infection recurrence, or treatment toxicity between the two groups. Therefore, this study supports the use of cefepime in cases with a minimal inhibitory concentration < 1 mg/l to spare carbapenems.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Biotechnology & Applied Microbiology
Hui-Shee Tan, Pan Yan, Hollysia Alda Agustie, Hwei-San Loh, Nabin Rayamajhi, Chee-Mun Fang
Summary: This study investigated 59 Enterobacteriaceae strains isolated from poultry fecal samples in Malaysia's commercial poultry farm, focusing on their resistance profile, phenotypic ESBL production, beta-lactamase genes, virulence factors, and plasmid replicon types. The study found that bla(TEM) and bla(CMY-2 group) were the most prevalent genes in Escherichia coli isolates, while bla(SHV), bla(DHA), and bla(TEM) were most commonly detected in Klebsiella spp. Resistance to extended spectrum cephalosporin was primarily mediated by plasmid-mediated AmpC beta-lactamase. The presence of plasmids in Enterobacteriaceae highlights their potential dissemination and the poultry as a reservoir of resistance and virulence determinants.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Environmental Sciences
Folake Temitope Fadare, Anthony Ifeanyi Okoh
Summary: The effluents of wastewater treatment plants in the Eastern Cape Province of South Africa contain a high abundance of multidrug-resistant Enterobacteriaceae, indicating a high antibiotic pressure environment. This highlights the need to optimize current WWTP processes to reduce potential risks of environmental contamination and health hazards.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2021)
Article
Public, Environmental & Occupational Health
Damian Rolbiecki, Monika Harnisz, Ewa Korzeniewska, Martyna Buta, Jakub Hubeny, Wiktor Zielinski
Summary: The study indicates that Klebsiella pneumoniae strains, including carbapenemase-producing, hyper-virulent strains, may be transmitted from wastewater to the upper respiratory tract of WWTP employees via bioaerosol emissions. The spread of drug resistance in the environment is significantly influenced by blaGES-type carbapenemases.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2021)
Article
Microbiology
Kobra S. Rizi, Ehsan Aryan, Masoud Youssefi, Kiarash Ghazvini, Zahra Meshkat, Yousef Amini, Hadi Safdari, Mohammad Derakhshan, Hadi Farsiani
Summary: The study investigated the role of AmpC enzymes in carbapenem resistance among clinical isolates of Escherichia coli and Klebsiella spp. producing AmpC/extended-spectrum ss-lactamases. Fifty-six AmpC-producing bacterial strains were examined, and antibiotic susceptibility tests were performed. The results showed that carbapenem resistance was present in AmpC-producing isolates with negative results for carbapenemase-encoding genes, highlighting the potential role of AmpC enzymes.
FUTURE MICROBIOLOGY
(2023)
Article
Microbiology
Hazrat Bilal, Gaojian Zhang, Tayyab Rehman, Jianxion Han, Sabir Khan, Muhammad Shafiq, Xuegang Yang, Zhongkang Yan, Xingyuan Yang
Summary: The study revealed the presence of various carbapenemases-producing K. pneumoniae strains in clinical samples from Pakistan, with a high prevalence of those carrying the bla(NDM-1) gene. These strains with different sequence types also showed some variation in cluster analysis. This study also identified IncX3 plasmids carrying the bla(NDM-1) gene for the first time in Pakistan, suggesting a potential new vehicle for disseminating bla(NDM-1) among Enterobacteriaceae due to its high transferability rate.
Article
Multidisciplinary Sciences
Wim L. Cuypers, Pieter Meysman, Francois-Xavier Weill, Rene S. Hendriksen, Getenet Beyene, John Wain, Satheesh Nair, Marie A. Chattaway, Blanca M. Perez-Sepulveda, Pieter-Jan Ceyssens, Tessa de Block, Winnie W. Y. Lee, Maria Pardos de la Gandara, Christian Kornschober, Jacob Moran-Gilad, Kees T. Veldman, Martin Cormican, Mia Torpdahl, Patricia I. Fields, Tomas Cerny, Liselotte Hardy, Bieke Tack, Kate C. Mellor, Nicholas Thomson, Gordon Dougan, Stijn Deborggraeve, Jan Jacobs, Kris Laukens, Sandra Van Puyvelde
Summary: This study provides a genomic overview of the population structure and antimicrobial resistance of Salmonella enterica serovar Concord. The researchers found that S. Concord is distributed among three Salmonella super-lineages and exhibits different levels of antimicrobial resistance and geographical distribution among different lineages.
NATURE COMMUNICATIONS
(2023)
Article
Infectious Diseases
Ananda Tiwari, Jaana Paakkanen, Ahmad I. Al-Mustapha, Juha Kirveskari, Rene S. Hendriksen, Annamari Heikinheimo
Summary: Analyzing samples of municipal wastewater influent can help to map the status of antibiotic-resistant bacteria (ARB) and predict the public health risks. In this study, bacterial isolates carrying beta-lactamase genes in wastewater were investigated and their characteristics were compared.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Public, Environmental & Occupational Health
Mwapu Dika Ndahi, Rene Hendriksen, Birgitte Helwigh, Roderick M. Card, Idowu Oluwabunmi Fagbamila, Oluwadamilola Olawumi Abiodun-Adewusi, Eme Ekeng, Victoria Adetunji, Ini Adebiyi, Jens Kirk Andersen
Summary: Indiscriminate use of antimicrobials by Nigerian poultry farmers without proper knowledge and practice is leading to the development of antibiotic resistance. The survey showed that farmers are using multiple classes of antibiotics, including unregistered ones, with high concentrations. This highlights the need for stronger antibiotics as a driver of antibiotic resistance.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2023)
Article
Infectious Diseases
Manuela Iurescia, Elena Lavinia Diaconu, Patricia Alba, Fabiola Feltrin, Carmela Buccella, Roberta Onorati, Angelo Giacomi, Andrea Caprioli, Alessia Franco, Antonio Battisti, Virginia Carfora
Summary: This study investigated the genomic characteristics of cfr-positive, multidrug-resistant livestock-associated MRSA in Italian pig holdings. The results showed that the isolates had different cfr variants, with one displaying linezolid resistance. The cfr gene was found on a novel plasmid in the 2008 CC1 isolate, while it was located on the chromosome in the 2010 CC398 isolate.
Article
Public, Environmental & Occupational Health
Elina Tast Lahti, Nadja Karamehmedovic, Hilde Riedel, Linnea Blom, Jeppe Boel, Elisabetta Delibato, Martine Denis, Alieda van Essen-Zandbergen, Aurora Garcia-Fernandez, Rene Hendriksen, Anna Heydecke, Angela H. A. M. van Hoek, Tom Huby, Renata Kwit, Claudia Lucarelli, Karl Lundin, Valeria Michelacci, Slawomir Owczarek, Isaac Ring, Jette Sejer Kjeldgaard, Ingegerd Sjogren, Milena Skora, Mia Torpdahl, Maria Ugarte-Ruiz, Kees Veldman, Eleonora Ventola, Magdalena Zajac, Cecilia Jernberg
Summary: Several proficiency tests and external quality assessments are available for evaluating laboratories' ability to detect and characterize enteropathogenic bacteria, but they are usually targeting specific sectors. This study aimed to assess the cross-sectoral capability of European laboratories in detecting and characterizing foodborne pathogens and provide recommendations for future assessments. The results confirmed the feasibility of using a cross-sectoral approach for assessing the joint capacity to detect and characterize foodborne pathogens.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Microbiology
Gabriele Arcari, Riccardo Polani, Stefania Santilli, Valerio Capitani, Federica Sacco, Francesco Bruno, Aurora Garcia-Fernandez, Giammarco Raponi, Laura Villa, Giuseppe Gentile, Alessandra Carattoli
Summary: In 2021, Klebsiella pneumoniae ST307 strains causing pulmonary and bloodstream infections in a hospital in Rome, Italy, exhibited high levels of resistance to ceftazidime-avibactam (CZA). One of these strains showed resistance to both CZA and carbapenems and carried two copies of bla(KPC-3) and one copy of bla(KPC-31) on plasmid pKpQIL.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Rene S. Hendriksen, Lina M. Cavaco, Beatriz Guerra, Valeria Bortolaia, Yvonne Agerso, Christina Aaby Svendsen, Hanne Norgaard Nielsen, Jette Sejer Kjeldgaard, Susanne Karlsmose Pedersen, Mette Fertner, Henrik Hasman
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Sidsel Nag, Gunhild Larsen, Judit Szarvas, Laura Elmlund Kohl Birkedahl, Gabor Mate Gulyas, Wojchiech Jakub Ciok, Timmie Mikkel Lagermann, Silva Tafaj, Susan Bradbury, Peter Collignon, Denise Daley, Victorien Dougnon, Kafayath Fabiyi, Boubacar Coulibaly, Rene Dembele, Georgette Nikiema, Natama Magloire, Isidore Juste Ouindgueta, Zenat Zebin Hossain, Anowara Begum, Deyan Donchev, Mathew Diggle, Leeann Turnbull, Simon Levesque, Livia Berlinger, Kirstine Kobberoe Sogaard, Paula Diaz Guevara, Carolina Duarte Valderrama, Panagiota Maikanti, Jana Amlerova, Pavel Drevinek, Jan Tkadlec, Milica Dilas, Achim Kaasch, Henrik Torkil Westh, Mohamed Azzedine Bachtarzi, Wahiba Amhis, Carolina Elisabeth Satan Salazar, JoseEduardo Villacis, Maria Angeles Dominguez Luzon, Damaris Berbel Palau, Claire Duployez, Maxime Paluche, Solomon Asante-Sefa, Mie Moller, Margaret Ip, Ivana Marekovic, Agnes Pal-Sonnevend, Clementiza Elvezia Cocuzza, Asta Dambrauskiene, Alexandre Macanze, Anelsio Cossa, Inacio Mandomando, Philip Nwajiobi-Princewill, Iruka N. Okeke, Aderemi O. Kehinde, Ini Adebiyi, Ifeoluwa Akintayo, Oluwafemi Popoola, Anthony Onipede, Anita Blomfeldt, Nora Elisabeth Nyquist, Kiri Bocker, James Ussher, Amjad Ali, Nimat Ullah, Habibullah Khan, Natalie Weiler Gustafson, Ikhlas Jarrar, Arif Al-Hamad, Viravarn Luvira, Wantana Paveenkittiporn, Irmak Baran, James C. L. Mwansa, Linda Sikakwa, Kaunda Yamba, Rene Sjogren Hendriksen, Frank Moller Aarestrup
Summary: The Two Weeks in the World research project has gathered a dataset of 3087 clinically relevant bacterial genomes with corresponding metadata, collected from 59 diagnostic units in 35 countries worldwide in 2020. A relational database is available, providing metadata and summary data from selected bioinformatic analysis, such as species prediction and identification of acquired resistance genes.
Article
Genetics & Heredity
Thea Kristensen, Lauge Holm Sorensen, Susanne Karlsmose Pedersen, Jacob Dyring Jensen, Hanne Mordhorst, Niamh Lacy-Roberts, Oksana Lukjancenko, Yan Luo, Maria Hoffmann, Rene S. Hendriksen
Summary: The global surveillance and outbreak investigation of antimicrobial resistance (AMR) is shifting from traditional biology to bioinformatics, thanks to developments in whole-genome sequencing (WGS) technologies, bioinformatics tools, and reduced costs. This study presents the results of the first iteration of the Genomic PT (GPT), which assessed laboratories' capacity to produce reliable WGS data. Most laboratories delivered high-quality sequence data, with only two laboratories identified as underperforming. The study highlights the importance of systematic quality control procedures to ensure reliable WGS data for surveillance and outbreak investigation.
MICROBIAL GENOMICS
(2023)
Article
Genetics & Heredity
Valeria Michelacci, Adrien Assere, Simone Caccio, Marina Cavaiuolo, Kirsten Mooijman, Stefano Morabito, Susanne Karlsmose Pedersen, Maroua Sayeb, Bo Segerman, Magnus Simonsson, Hanna Skarin, Rosangela Tozzoli, Angela van Hoek, Rene Sjogren Hendriksen
Summary: The Inter European Union Reference Laboratories (EURLs) Working Group on Next Generation Sequencing (NGS) has been working since 2017 to promote the adoption of NGS by the National Reference Laboratories (NRLs) in the European Union. By releasing guidance documents and working together, the EURLs have aimed to improve preparedness in the use of NGS for characterizing microbial hazards and tracing infection sources.
MICROBIAL GENOMICS
(2023)
Article
Microbiology
Gordan Kompes, Sanja Duvnjak, Irena Reil, Rene S. Hendriksen, Lauge Holm Sorensen, Maja Zdelar-Tuk, Boris Habrun, Luka Cvetnic, Antonela Bagaric, Silvio Spicic
Summary: In 2021, a multi-drug resistant E. coli strain with the mcr-1 gene was isolated from pig in Croatia. The isolate showed resistance to multiple antimicrobial classes and harbored various resistance genes and virulence genes. The mcr-1 gene was located within a conjugative plasmid, highlighting the importance of identifying mcr-1-carrying E. coli isolates in Croatia, especially in food-producing animals.
Article
Infectious Diseases
Daniela Fortini, Aurora Garcia-Fernandez, Claudia Lucarelli, Anna Maria Dionisi, Sergio Arena, Slawomir Owczarek, Michele Equestre, Alessandra Carattoli, Federica Sacco, Stefano Rossi, Roberta Ortenzi, Sara Primavilla, Laura Villa
Summary: In this study, a Salmonella enterica strain with reduced susceptibility to meropenem was isolated from a urinary infection. Genomic characteristics, pathogenicity, and antimicrobial resistance mechanisms were investigated. The strain carried two plasmids, one of which contained a resistance gene. Comparative analysis showed a high similarity between the plasmid and a known reference plasmid, but with a missing region that caused loss of conjugation ability. This is the first report of a plasmid carrying a resistance gene in Salmonella in our country, highlighting its importance for public health.
Article
Microbiology
Patricia Alba, Virginia Carfora, Fabiola Feltrin, Elena Lavinia Diaconu, Luigi Sorbara, Elena Dell'Aira, Tamara Cerci, Angela Ianzano, Valentina Donati, Alessia Franco, Antonio Battisti
Summary: The increasing prevalence of multidrug-resistant S. Infantis carrying pESI(like) megaplasmid in Europe is a major concern. This study sequenced and analyzed five prototype pESI(like) plasmids and found that although they have almost identical sequences, their structures vary, indicating different insertion or transposition events. These findings are essential for understanding the plasticity and evolution of pESI(like) megaplasmid and can contribute to risk management and policy decisions in combating antimicrobial resistance and multidrug resistance in food-borne pathogens.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Public, Environmental & Occupational Health
Mabel Kamweli Aworh, Jacob K. P. Kwaga, Rene S. Hendriksen, Emmanuel C. Okolocha, Erin Harrell, Siddhartha Thakur
Summary: This study aimed to investigate quinolone resistance in Escherichia coli isolated from poultry workers, chickens, and poultry farm/market environments in Abuja, Nigeria. The results showed that PMQR genes were prevalent in E. coli isolates, and PMCR genes were detected in PMQR-positive isolates originating from manure and drinking water. The coexistence of ESBL genes with qnrS-positive isolates was also observed. These findings highlight the public health implications of the horizontal transfer of PMQR genes among E. coli isolates in the human-poultry-environment interface.
ONE HEALTH OUTLOOK
(2023)
