Article
Biotechnology & Applied Microbiology
Xufang Shen, Hongwei Yan, Jieming Jiang, Weiyuan Li, Yuyu Xiong, Qi Liu, Ying Liu
Summary: The study compared the differential transcriptomic responses of the Takifugu rubripes larvae brain after E-2 treatment, revealing numerous differentially expressed genes and enriched metabolic pathways and signaling pathways. This provides important clues for further research on the possible involvement of the brain-pituitary-gonadal axis network in sex differentiation in T. rubripes.
Article
Biochemistry & Molecular Biology
Fan-Xiang Gao, Wei-Jia Lu, Yan Shi, Hao-yong Zhu, Yao-hui Wang, Han-qing Tu, Yang Gao, Li Zhou, Jian-Fang Gui, Zhe Zhao
Summary: Hybridization is an efficient method to breed new strains of aquatic animals. The present study produced a hybrid puffer with growth superiority by crossing female obscure puffer with male tiger puffer. Comparative transcriptome analysis revealed that genes related to cell proliferation, differentiation, and protein synthesis showed higher expression levels in the hybrid puffer, while genes associated with gametogenesis and energy metabolism were highly expressed in the obscure puffer. The identification of candidate genes like fn1a, ptprc, plcg2, etc., provide insights into the molecular basis of growth superiority in the hybrid puffer.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2021)
Article
Fisheries
Fengqin Shang, Mingxiu Bao, Fujun Liu, Ziwen Hu, Shengnan Wang, Xiao Yang, Yundeng Yu, Hongbin Zhang, Chihang Jiang, Xuemei Qiu, Yang Liu, Xiuli Wang
Summary: Through transcriptomic analysis, this study revealed the biological processes and molecular mechanisms of hypoxia in Takifugu rubripes. Hypoxia downregulated genes associated with the immune system and inflammation, but promoted dissolved oxygen uptake through angiogenesis and altered the expression patterns of genes involved in the inhibition of cell growth and proliferation.
Article
Genetics & Heredity
Fengqin Shang, Yun Lu, Yan Li, Bing Han, Renjie Wei, Shengmei Liu, Ying Liu, Yang Liu, Xiuli Wang
Summary: This study reveals the expression patterns of genes involved in the hypoxic response in Takifugu rubripes brains using RNA-seq analysis. It shows that carbohydrate, lipid, and amino acid metabolism are significant pathways for the enrichment of differentially expressed genes (DEGs) and discusses the importance of other biological processes such as the immune system and signal transduction. The findings provide valuable resources for studying the energy metabolism mechanism of hypoxia response in this species.
Article
Fisheries
Jian-xin Cheng, Yu-qing Xia, Ya-fang Liu, Peng-fei Liu, Ying Liu
Summary: Takifugu rubripes and Dicentrarchus labrax are important commercial fish in China, threatened by Cryptocaryon irritans. Transcriptome analysis revealed immune-related pathways enriched in response to C. irritans infection, providing insights into host resistance mechanisms.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Yu-qing Xia, Xin-tong Hua, Shuang Zhu, Ying Liu, Peng-fei Liu
Summary: The study cloned a partial C8 beta gene from pufferfish and found that it is expressed in various tissues with predominant expression in the liver. The expression of C8 beta was significantly upregulated in the liver after LPS stimulation. In larvae, C8 beta mRNA expression peaked during the hatching prophase and decreased significantly afterwards. These results suggest that C8 beta may play a role in anti-bacterial response and development in pufferfish.
AQUACULTURE REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
He Zhou, Yuqing Sun, Xin Li, Ziyu Zhou, Kexin Ma, Wenxuan Guo, Yuting Liang, Xingyi Xie, Jingxian Zhang, Qian Wang, Yang Liu
Summary: Our study analyzed the differences in transcriptomes of normal and sex-reverse Takifugu rubripes, identifying 13 differentially expressed genes related to sex differentiation. This is the first report on the gonadal transcriptome of pseudo-males in Takifugu rubripes, offering insights into the molecular mechanisms of masculinization induced by low-temperature treatment in cultured fish.
Article
Fisheries
Zhifeng Liu, Aijun Ma, Chenhao Yuan, Liguang Zhu, Haowen Chang, Lanliang Yu, Xuesong Meng
Summary: This study compared the kidney transcriptomes of tiger puffers at 5°C and 23°C to uncover the key pathways and genes involved in the response to low temperature stress. The results suggest that the arginine and proline metabolic pathways play a crucial role in the cold resistance of tiger puffers.
AQUACULTURE REPORTS
(2022)
Article
Immunology
Lei Gao, Ziyang Zhang, Zhen Xing, Qingsong Li, Ning Kong, Lingling Wang, Linsheng Song
Summary: Intestinal autochthonous bacteria play important roles in maintaining physiological homeostasis and host immune system. This study analyzed the variation of bacterial communities in the intestinal tract of tiger pufferfish and seawater in different aquaculture systems. Proteobacteria was the most dominant phyla, and Arcobacter was the most core bacterial taxon in the intestinal community. The differences between intestinal and seawater bacterial communities increased during the aquaculture process, and certain bacterial taxa were correlated with immune response parameters. This research is of great scientific significance for understanding intestinal bacterial homeostasis and biosecurity practice in pufferfish aquaculture.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Danni Zhang, Ni Yang, Ian D. Fisk, Jintao Li, Yuan Liu, Wenli Wang
Summary: The study investigates the effects of different cooking processes on the sensory properties of Takifugu rubripes, revealing significant changes in aroma and mouthfeel attributes due to thermodynamic alterations during cooking. These findings provide valuable insights for optimizing cooking conditions to enhance the desirable fish flavor.
Article
Environmental Sciences
Zhifeng Liu, Liguang Zhu, Xinan Wang, Shiying Liu, Aijun Ma, Haowen Chang, Zhibin Sun, Fei Xu, Haichi Zhao
Summary: The transcriptome analysis of tiger puffer livers at different temperatures revealed key pathways and genes involved in the response to low-temperature stress. Low temperatures caused digestive and metabolic disorders, but also triggered adaptive changes to cope with the cold. Up-regulated genes in the pancreatic secretion pathway, arginine and proline metabolism pathway, and circadian rhythm pathway played important roles in the survival, growth, and development of tiger puffers under low-temperature stress.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Guoyu Wang, Zhihao Wu, Lijuan Wang, Yuyu Xiong, Feng You
Summary: The present study investigated the effects of red light on the eyes and brain of the tiger puffer Takifugu rubripes. The results showed that red light filtering significantly decreased the activity of superoxide dismutase and catalase, and increased the content of cortisol. This study provides new insights into the role of red light in promoting ovulation in mature fish.
AQUACULTURE REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Leilei Tan, Qian Liu, Yangbin He, Jingjing Zhang, Jilun Hou, Yuqin Ren, Wenxiu Ma, Qian Wang, Changwei Shao
Summary: In this study, a spermatogenic stem cell line was established from tiger puffer fish, which showed stable growth and the ability to differentiate into other types of cells. This research provides an important research model and foundation for sex determination and breeding control in fish.
Article
Fisheries
XinAn Wang, AiJun Ma
Summary: This study measured the activities of antioxidant factors in the livers of Takifugu rubripes at different temperatures and analyzed the effects of genotype x temperature interactions on the antioxidant activities. The results showed that genotype, temperature, and genotype x temperature interactions significantly affected antioxidant activities. The activity of SOD was highest at lower temperatures, while the activity of GPX was highest at higher temperatures.
Article
Fisheries
Xinan Wang, Aijun Ma, Jingkun Yang
Summary: Genetic parameters of three antioxidant factors in Takifugu rubripes were evaluated, showing low heritability and high full-sib family effect for SOD, CAT, and GPX, with positive correlations among these enzymes. Therefore, genome-wide selection and CRISPR could be used for genetic improvement, while multi-trait integrated breeding technology or indirect selection could enhance antioxidant competence in cultured fish by genetically improving these three antioxidant traits.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Evolutionary Biology
Boyang Hu, Yuan Tian, Qi Li, Shikai Liu
Summary: This study investigated the genomic signatures of long-term selective breeding in Pacific oysters, revealing the impact of artificial selection on genetic diversity and genomic features. Population structure analysis and identification of selective sweeps highlighted the genetic differences between fast-growing oyster strains and wild populations. The findings provide valuable insights for understanding the genetic basis of growth regulation in Pacific oysters and will be beneficial for future genome-assisted breeding programs.
EVOLUTIONARY APPLICATIONS
(2022)
Article
Fisheries
Ben Yang, Shangyu Zhai, Fuqiang Zhang, Hebing Wang, Liting Ren, Yongjing Li, Qi Li, Shikai Liu
Summary: This study conducted a genome wide association study in Pacific oysters to identify genetic loci underlying resistance to bacterial Vibrios. It revealed a polygenic architecture of resistance traits and identified 18 SNP loci and three QTL underlying the trait of resistance to Vibrio. In total, 178 genes involved in various processes of immune response were identified, providing valuable genetic resources for genomic selection breeding of Vibrio-resistant strains in the C. gigas.
Article
Biotechnology & Applied Microbiology
Yulin Jin, Tao Zhou, Wansheng Jiang, Ning Li, Xiaoyan Xu, Suxu Tan, Huitong Shi, Yujia Yang, Zihao Yuan, Wenwen Wang, Guyu Qin, Shikai Liu, Dongya Gao, Rex Dunham, Zhanjiang Liu
Summary: This study identified genomic regions associated with resistance against enteric septicemia of catfish (ESC) and found different mechanisms of resistance in the liver and intestine. The study also highlighted the importance of apolipoproteins and downregulation of NCK in ESC resistance.
MARINE BIOTECHNOLOGY
(2022)
Article
Physiology
Youhui Gong, Ting Li, Qi Li, Shikai Liu, Nannan Liu
Summary: The increasing resistance of mosquitoes to insecticides poses a major threat to control efforts worldwide. In this study, multiple P450 genes were found to be up-regulated in permethrin resistant mosquitoes, indicating their role in the development of resistance. Additionally, the overexpression of the cytochrome P450-reductase (CPR) gene in permethrin resistant mosquitoes suggests its importance in the detoxification system and metabolism of permethrin.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Genetics & Heredity
Sai Wan, Qi Li, Hong Yu, Shikai Liu, Lingfeng Kong
Summary: This study identifies the involvement of the PPAR/RXR heterodimer in the regulation of carotenoid metabolism in molluscs, providing insights into the evolution and conservation of this nuclear receptor heterodimer.
Article
Genetics & Heredity
Sai Wan, Qi Li, Hong Yu, Shikai Liu, Lingfeng Kong
Summary: Carotenoids are essential micronutrients for oysters and other animals, and the molecular mechanisms and related genes involved in carotenoid processing are still limited. In this study, a comparative transcriptome analysis of Pacific oysters was performed, revealing differentially expressed genes related to carotenoid transport, storage, cleavage, and retinoid homeostasis. Down-regulation of apoli-poprotein genes significantly decreased the carotenoid level in the oyster's digestive gland, suggesting their role in carotenoid transport and storage. These findings provide insights into the molecular basis of carotenoid metabolism and functions of carotenoid-related genes in oysters.
Article
Biochemistry & Molecular Biology
Yujia Yang, Tao Zhou, Yang Liu, Changxu Tian, Lisui Bao, Wenwen Wang, Yu Zhang, Shikai Liu, Huitong Shi, Suxu Tan, Dongya Gao, Rex A. Dunham, Zhanjiang Liu
Summary: This study investigates the mechanisms of sex determination in channel catfish and identifies differentially methylated CpG sites and differentially expressed genes within the sex determination region (SDR). Methylation differences were primarily located at nucleotide position 14.0-20.3 Mb of the sex chromosome, suggesting a potential role of methylation modification in sex determination in channel catfish. These findings provide insights into the genetic and epigenetic factors involved in sex determination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Xin Li, Ben Yang, Chenyu Shi, Hebing Wang, Ruihai Yu, Qi Li, Shikai Liu
Summary: This study investigates the synergistic effect of low salinity stress and pathogen infection on the mortality of Pacific oysters. The results show that low salinity stress affects microbial composition and immune response of infected oysters, suggesting that it may trigger immune dysregulation and contribute to oyster mass mortality.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Huihui Wang, Hong Yu, Qi Li, Shikai Liu
Summary: This study investigates the expression profiles of lncRNA in the gonads of Pacific oyster and predicts their potential functions in the regulatory mechanisms of gametogenesis. The results suggest that trans-regulatory lncRNAs play a vital role in the reproductive process. By constructing core networks of lncRNAs-mRNAs, several important lncRNAs are identified to regulate sterility-related genes. This research provides insights into the regulatory mechanism of lncRNAs in the gametogenesis of sterile triploid oysters.
Article
Fisheries
Yiming Hu, Qi Li, Chengxun Xu, Shikai Liu, Lingfeng Kong, Hong Yu
Summary: This study investigates the genetic diversity and structure of three generations of mass-selected lines and three wild populations of C. nippona. The results show that the genetic diversity of the selected lines is maintained, with minimal genetic differentiation among different lines. Significant genetic differentiation is observed among the wild populations, suggesting the presence of genetically isolated populations. To maximize future selective breeding efforts, the use of larger broodstock and a multi-line breeding strategy is recommended.
Article
Fisheries
Jianwen Gong, Qi Li, Hong Yu, Shikai Liu, Lingfeng Kong
Summary: This study investigated the physiological and immunological response of Crassostrea nippona to low salinity. The results showed that low salinity led to hyperosmolality in the hemolymph and differential gene expression in the gill transcriptome. The study provides insights into the immune mechanism of C. nippona under low salinity stress.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yiming Hu, Qi Li, Chengxun Xu, Shikai Liu, Lingfeng Kong, Hong Yu
Summary: This study demonstrates the feasibility of improving the growth traits of the Iwagaki oyster through selective breeding, with significant increases in selection response and genetic gain observed.
Article
Fisheries
Yameng He, Xin Li, Chenyu Shi, Yin Li, Qi Li, Shikai Liu
Summary: In this study, RNA-seq analysis of Pacific oyster gill tissue was performed to investigate the immune response to bacterial infection. The results showed that the immune strategy of the oyster changed significantly at short-term and long-term stages, with activation of immune-related genes with different functions. This work provides valuable insights into immune response, disease monitoring, and disease resistance breeding.
Article
Genetics & Heredity
Kuiqin Wang, Ben Yang, Qi Li, Shikai Liu
Summary: This study systematically compared ten genomic-prediction algorithms and validated them using genotype and phenotype data of five aquaculture species. The results showed that reproducing kernel Hilbert space and support-vector machine algorithms performed well in predicting most traits. The study also developed an R package to assist genomic selection breeding in aquaculture species.
Article
Engineering, Marine
Yiming Hu, Qi Li, Chengxun Xu, Shikai Liu, Lingfeng Kong, Hong Yu
Summary: This study investigated the differences in temperature and salinity tolerance between C. nippona and C. gigas spat and determined the optimal environmental conditions for the aquaculture of C. nippona spat. The results showed that C. nippona had lower survival rates than C. gigas under temperature changes and salinity decreases, but higher survival rates with increasing salinity. The findings suggest that particular attention should be paid to the inferior adaptability of C. nippona when farming outside their natural habitats.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
