Article
Infectious Diseases
Cyrille Ngassam Tchamba, Jean-Noel Duprez, Pierrick Lucas, Yannick Blanchard, Filip Boyen, Freddy Haesebrouck, Maria A. Argudin, Jacques Mainil, Damien Thiry
Summary: This study identified and compared the SCCmec types of MRSA or MRNAS isolated from different countries in various animal species and humans. The results confirmed a high prevalence of SCCmec types IV and V in LA-MRS and CA-MRS in Belgium, highlighting the possible public health hazard and absence of SCCmec in some MRNAS.
Article
Multidisciplinary Sciences
Manoshi S. Datta, Idan Yelin, Ori Hochwald, Imad Kassis, Liron Borenstein-Levin, Amir Kugelman, Roy Kishony
Summary: Through whole-genome sequencing of S. epidermidis isolates from premature infants, the study reveals patient-specific colonization signatures and rapid gain and loss of the antibiotic resistance gene mecA via diverse structural variants.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Nathita Phumthanakorn, Thidathip Wongsurawat, Piroon Jenjaroenpun, Alongkorn Kurilung, Nuvee Prapasarakul
Summary: This study analyzed the SCCmec element in clinical isolates from sick dogs, revealing complex and diverse structures and genetic content, with novel organization.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Jo-Ann Mcclure, John M. Conly, Kunyan Zhang
Summary: SCCmec, predominantly found in methicillin-resistant Staphylococcus aureus, has been shown to have a larger diversity of elements in coagulase-negative staphylococci (CoNS). A clinical strain of Staphylococcus hominis was found to carry a composite SCCmec element with an uncharacterized ccr1 and type A mec gene combination, along with a secondary element containing ccr4. The complex structure of the element suggests a high level of recombination in SCCmec in CoNS.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Christian Kranjec, Sofie S. Kristensen, Karolina T. Bartkiewicz, Mikkel Bronner, Jorunn P. Cavanagh, Aparna Srikantam, Geir Mathiesen, Dzung B. Diep
Summary: A novel hybrid bacteriocin H1 showed superior antimicrobial effects against Staphylococcus haemolyticus and high activity towards various strains without strain-dependency. Combination with broad-spectrum bacteriocins micrococcin P1 and garvicin KS effectively eradicated biofilm-associated cells and prevented development of resistant mutants, indicating its potential as a treatment option for S. haemolyticus.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Nathita Phumthanakorn, Sybille Schwendener, Valentina Dona, Pattrarat Chanchaithong, Vincent Perreten, Nuvee Prapasarakul
Summary: This study conducted genomic comparative analyses of MRSP isolates from dogs and humans, revealing differences in prophage distribution and identification of a novel composite pathogenicity island in ST181 isolates. Among the different STs, ST45 isolates showed the highest genomic diversity, suggesting potential host adaptation leading to different colonization patterns.
Article
Multidisciplinary Sciences
Mansour Mohamadou, Sarah Riwom Essama, Marie Chantal Ngonde Essome, Lillian Akwah, Nudrat Nadeem, Hortense Gonsu Kamga, Sadia Sattar, Sundus Javed
Summary: The prevalence of MRSA infections in Northern Cameroon is high, with most MRSA isolates being multidrug-resistant and representing community-acquired strains.
Article
Multidisciplinary Sciences
Mansour Mohamadou, Sarah Riwom Essama, Marie Chantal Ngonde Essome, Lillian Akwah, Nudrat Nadeem, Hortense Gonsu Kamga, Sadia Sattar, Sundus Javed
Summary: This study reveals a high prevalence of MRSA infections among Staphylococcus aureus infections in northern Cameroon, with MRSA strains being highly resistant to multiple drugs.
Article
Immunology
Xinyi Wang, Huilin Zhao, Bingjie Wang, Ying Zhou, Yanlei Xu, Lulin Rao, Wenxiu Ai, Yinjuan Guo, Xiaocui Wu, Jingyi Yu, Longhua Hu, Lizhong Han, Shuying Chen, Liang Chen, Fangyou Yu
Summary: This study characterized six ST8 strains collected from three tertiary hospitals in China, and found that two MRSA strains exhibited similar virulence potential as the epidemic USA300 strain. These findings highlight the need for enhanced surveillance of these highly virulent MRSA strains in China.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Infectious Diseases
Faiqa Arshad, Sidrah Saleem, Romeeza Tahir, Shah Jahan, Kokab Jabeen, Aneela Khawaja, Zille Huma, Muhammad Roman, Faheem Shahzad
Summary: This study identified and characterized MRSA strains exhibiting vancomycin MIC creep phenomenon by analyzing SCCmec and MLVA. SCCmec III was found to be the most common type in MRSA isolates showing vancomycin resistance, and the presence of SCCmec XI may pose additional challenges for infection control.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Immunology
Masakaze Hamada, Tetsuo Yamaguchi, Ayami Sato, Daisuke Ono, Kotaro Aoki, Chiaki Kajiwara, Soichiro Kimura, Tadashi Maeda, Masakazu Sasaki, Hinako Murakami, Yoshikazu Ishii, Kazuhiro Tateda
Summary: The study revealed that MRSA strains isolated from bloodstream infections, especially SCCmec IV strains, demonstrated increased ability to form plasma-biofilms, which were mainly composed of proteins.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Mais Maree, Le Thuy Thi Nguyen, Ryosuke L. Ohniwa, Masato Higashide, Tarek Msadek, Kazuya Morikawa
Summary: This study demonstrates that natural transformation plays a role in the transfer of SCCmec and possibly other mobile genetic elements in S. aureus biofilms, and biofilm growth conditions enhance the efficiency of natural transformation in S. aureus.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Etinosa O. Igbinosa, Abeni Beshiru, Isoken H. Igbinosa, Abraham G. Ogofure, Temitope C. Ekundayo, Anthony I. Okoh
Summary: The presence of methicillin-resistant Staphylococcus aureus (MRSA) in meat in Nigeria is a concern for public health.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Genetics & Heredity
G. K. Sivaraman, K. H. Muneeb, S. Sudha, Bibek Shome, Mark Holmes, Jennifer Cole
Summary: The study identified multi-drug resistant S. haemolyticus in fishes, emphasizing the importance of continuous monitoring of MRSH in food. It also discussed the role of prudent hygienic practices in flattening the antibiotic resistance curve.
Article
Infectious Diseases
Feiteng Zhu, Hemu Zhuang, Lingfang Di, Zhengan Wang, Yiyi Chen, Shengnan Jiang, Chao Gu, Lu Sun, Haiping Wang, Yiwei Zhu, Peng Lan, Dandan Wu, Yunsong Yu, Shujuan Ji, Yan Chen
Summary: This study evaluated the susceptibility of 472 methicillin-resistant Staphylococcus aureus (MRSA) isolates to ceftobiprole (BPR) and investigated the mechanisms underlying BPR resistance. The results showed that community-associated MRSA isolates were more susceptible to BPR than healthcare-associated MRSA isolates. Nanopore sequencing revealed SCCmec repeat-mediated resistance mechanism in BPR-resistant MRSA isolates, and mutations in multiple genes were found to potentially contribute to BPR-resistance development in MRSA.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Microbiology
Shengnan Jiang, Hemu Zhuang, Feiteng Zhu, Xiang Wei, Junxiong Zhang, Lu Sun, Shujuan Ji, Haiping Wang, Dandan Wu, Feng Zhao, Rushuang Yan, Yunsong Yu, Yan Chen
Summary: The emergence of daptomycin-resistant Staphylococcus aureus strains is a global problem caused by point mutations in the mprF gene. This study found that specific mprF mutations lead to the seesaw effect of distinct beta-lactams in MRSA strains and the combination therapy of daptomycin and beta-lactams has synergistic activity. The cell surface positive charge is not affected by mprF mutations. Understanding the mechanism of restoring susceptibility to beta-lactam antibiotics mediated by mprF mutations is important for the treatment of MRSA infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biochemical Research Methods
Zhi Ruan, Shengmei Zou, Zeyu Wang, Luhan Zhang, Hangfei Chen, Yuye Wu, Huiqiong Jia, Mohamed S. Draz, Ye Feng
Summary: A new tool called Metagenomic Intra-species Typing (MIST) was introduced for pathogen detection and strain-level analysis. The tool accurately predicted strain composition and identified high-risk clones in infected body fluids. Multiple-strain infections were found to be more prevalent than previously thought.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Infectious Diseases
Yan Chen, Lu Sun, Xiaoliang Ba, Shengnan Jiang, Hemu Zhuang, Feiteng Zhu, Haiping Wang, Peng Lan, Qiucheng Shi, Zhengan Wang, Yiyi Chen, Keren Shi, Shujuan Ji, Yan Jiang, Mark A. Holmes, Yunsong Yu
Summary: This study investigated the genomic epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in China and found a high prevalence of the CC59 lineage in community-associated MRSA. Almost all isolates in the CC59 lineage showed genetic variants leading to increased beta-lactam susceptibility, suggesting potential for improved antibiotic stewardship.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Yue Li, Yiwei Zhu, Wanqing Zhou, Zhongju Chen, Robert A. Moran, Huanhuan Ke, Yu Feng, Willem van Schaik, Han Shen, Jingshu Ji, Zhi Ruan, Xiaoting Hua, Yunsong Yu
Summary: This study characterized AFM-2 and AFM-3 metallo-beta-lactamase from clinical Pseudomonas aeruginosa isolates. The results showed that these enzymes exhibited beta-lactamase activity comparable to NDM-1, and the co-carriage of bla(AFM) and bla(KPC) genes rendered P. aeruginosa resistant to antipseudomonal beta-lactams. Furthermore, the association of bla(AFM) genes with translocatable genetic elements and plasmids highlighted their potential for dissemination.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Feiteng Zhu, Hemu Zhuang, Lingfang Di, Zhengan Wang, Yiyi Chen, Shengnan Jiang, Chao Gu, Lu Sun, Haiping Wang, Yiwei Zhu, Peng Lan, Dandan Wu, Yunsong Yu, Shujuan Ji, Yan Chen
Summary: This study evaluated the susceptibility of 472 methicillin-resistant Staphylococcus aureus (MRSA) isolates to ceftobiprole (BPR) and investigated the mechanisms underlying BPR resistance. The results showed that community-associated MRSA isolates were more susceptible to BPR than healthcare-associated MRSA isolates. Nanopore sequencing revealed SCCmec repeat-mediated resistance mechanism in BPR-resistant MRSA isolates, and mutations in multiple genes were found to potentially contribute to BPR-resistance development in MRSA.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Danni Bao, Linyao Huang, Jianxin Yan, Yexuzi Li, Zhi Ruan, Tian Jiang
Summary: This study described the genomic characteristics of a P. putida isolate co-carrying five beta-lactam resistance genes recovered from a urinary tract infection in China. The isolate showed resistance to a wide range of antimicrobial agents and could be assigned to a new sequence type. The presence of multiple beta-lactamase genes suggests that this isolate has the potential to be an antibiotic resistance reservoir for Pseudomonas spp.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Linna Xu, Fen Wan, Hao Fu, Biao Tang, Zhi Ruan, Yonghong Xiao, Qixia Luo
Summary: In our study, we identified mcr-10-carrying isolates, including multidrug-resistant ST11 Klebsiella pneumoniae isolates coharboring mcr-10 and mcr-8 genes, which are resistant to polymyxins and show reduced susceptibility to tigecycline. Through screening 941,449 bacterial genomes in the GenBank database, we found a sporadic distribution of mcr-10-harboring isolates worldwide in various sources, especially in humans, highlighting the need for close monitoring and concern in clinical settings.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Ping Miao, Yiming Jiang, Ying Jian, Jiali Shi, Yao Liu, Pipat Piewngam, Yue Zheng, Gordon Y. C. Cheung, Qian Liu, Michael Otto, Min Li
Summary: Allergic rhinitis (AR), commonly known as hay fever, is a widespread condition that greatly impacts the lives of millions of people. This study explores the role of nasal microbiota in AR and reveals distinct differences in the nasal microbiome between AR patients and healthy individuals. Specifically, the study identifies Streptococcus salivarius as a contributing factor to AR development and highlights the potential of targeted antibacterial approaches for AR therapy.
NATURE MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Yuye Wu, Tian Jiang, Danni Bao, Meina Yue, Huiqiong Jia, Jianyong Wu, Zhi Ruan
Summary: Due to the frequent international and intercontinental transmission of multidrug-resistant bacteria, it is imperative to understand the epidemiology, phylogeography, and population structure of carbapenem-resistant Salmonella enterica (CRSE) across the globe. Our findings on the worldwide spread and transmission dynamics of this emerging bacterium has increased the knowledge of its global epidemics. Continued epidemiological surveillance is necessary to prevent global outbreak of multidrug-resistant Salmonella infections.
DRUG RESISTANCE UPDATES
(2023)
Article
Infectious Diseases
Yiyi Chen, Lu Sun, Yueqin Hong, Mengzhen Chen, Hao Zhang, Yaqin Peng, Kang Liao, Haiping Wang, Feiteng Zhu, Hemu Zhuang, Zhengan Wang, Shengnan Jiang, Yunsong Yu, Yan Chen
Summary: The overuse of antibiotics in livestock is contributing to the burden of antimicrobial resistance in humans, and the emergence of LA-MRSA is a growing concern. This study identified the resistance mechanisms and genetic features of clinical ST9 MRSA isolates and found that they were multidrug-resistant and resistant to third-generation tetracyclines. Phylogenetic analysis revealed a potential transmission of ST9 isolates between humans and livestock. The study highlights the importance of adopting a One Health approach to control antibiotic resistance.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Seth W. Dickey, Dylan J. Burgin, Steven Huang, David Maguire, Michael Otto
Summary: Diverse organisms secrete amphipathic biomolecules for competitive gains. However, how cells cope with producing these membrane-permeabilizing molecules is unclear. We focused on the PSM family of secreted amphipathic peptides in the pathogen Staphylococcus aureus that uses two ABC transporters, PmtCD and AbcA, to export peptides across the bacterial cell membrane. We found that increased peptide hydropho-bicity favors PSM secretion through PmtCD over AbcA and that only PmtCD protected cells against amphipathic peptides. We propose a two-system model in which PmtCD and AbcA independently export PSMs from either membrane or cytosolic environments, respectively. Our model provides a rationale for the encoding of multiple transport systems on diverse biosynthetic gene clusters used to produce distinct amphipathic molecules. In addition, our data serve as a guide for selectively blocking PSM secretion to achieve antimicrobial or antivirulence approaches and to disrupt established roles of PSM-mediated virulence.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biotechnology & Applied Microbiology
Lei He, Huiying Lv, Yanan Wang, Feng Jiang, Qian Liu, Feiyang Zhang, Hua Wang, Hao Shen, Michael Otto, Min Li
Summary: Quorum cheating, a mutation in cell density-sensing systems, has become a crucial factor in biofilm-associated infection. Inactivation of the quorum-sensing system in Staphylococcus aureus leads to enhanced biofilm formation and resistance to antibiotics and immune defense. Antibiotic treatment can promote quorum cheating and the development of biofilms, especially in biofilm infection models.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Infectious Diseases
Mariana Favero Bonesso, Carlos Magno Castelo Branco Fortaleza, Ricardo de Souza Cavalcante, Moises Teixeira Sobrinho, Carlos Fernando Ronchi, Ligia Maria Abraao, Hwang-Soo Joo, Michael Otto, Maria de Lourdes Ribeiro de Souza da Cunha
Summary: This study aimed to investigate the molecular epidemiology of Staphylococcus aureus in patients on mechanical ventilation and its role in the development of ventilator-associated pneumonia (VAP). Various factors, including resistance profile, virulence factors, and clonal profile, were analyzed in the S. aureus isolates. The study identified patient-related risk factors for VAP and found that the majority of strains causing VAP originated from the community.
Review
Infectious Diseases
Lauren R. Heinzinger, Aaron R. Pugh, Julie A. Wagner, Michael Otto
Summary: Antibiotic resistance is a global threat to human and animal health, especially with the increasing prevalence of antibiotic-resistant Staphylococcus aureus strains. Bacteriocins, which are antimicrobial peptides produced by bacteria, offer a potential alternative therapeutic that has shown efficacy in animal models.
Letter
Infectious Diseases
Yuye Wu, Chenghao Wu, Danni Bao, Huiqiong Jia, Mohamed S. Draz, Fang He, Zhi Ruan
LANCET INFECTIOUS DISEASES
(2022)
