Article
Oncology
Zhichao Li, Haibo Xu, Lei Yu, Jia Wang, Qian Meng, Hongbing Mei, Zhiming Cai, Wei Chen, Weiren Huang
Summary: This research established an effective three-dimensional culture system for generating kidney cancer organoids and successfully generated 33 organoid lines from different subtypes of kidney cancer. These organoids preserved the histological architecture, mutational landscape, and transcriptional profile of the parental tumor tissues, and revealed inter- and intra-tumoral heterogeneity. Additionally, the organoids can be used for drug screening and evaluating the efficacy of immunotherapy.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
AhmadReza Rezaeian, Fatemeh Khatami, Saeed Heidari Keshel, Mohammad Reza Akbari, Akram Mirzaei, Keykavos Gholami, Reza Mohammadi Farsani, Seyed Mohammad Kazem Aghamir
Summary: This study demonstrates that MSC-exosomes have certain anti-tumor effects on prostate, bladder, and kidney cancer cells. These effects are mainly achieved by inducing apoptosis and inhibiting cancer cell proliferation.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Elisa Landucci, Domenico E. Pellegrini-Giampietro, Fabrizio Facchinetti
Summary: Term neonatal hypoxic-ischemic encephalopathy (HIE) is an important cause of long-term disability and further research is needed to develop new treatments. When choosing a research model, it is important to consider different experimental models to test the effectiveness of new drugs. Therapeutic hypothermia is already a routine treatment for neonatal encephalopathy, so the selected model must undergo hypothermia to test the translational effects of new or repurposed drugs. Translational research requires initiating treatment after the induction of insult and evaluating the time window for intervention to translate to clinical practice. Hippocampal organotypic slice cultures are a valuable intermediate between cell lines and in vivo models. In vivo models, particularly rodent models, offer flexibility and cost-effectiveness for dose-response testing of pharmacological agents. Large animal models can be used for focused and accurate translational studies, including pharmacokinetics and safety assessments.
Article
Chemistry, Multidisciplinary
Gwang-Bum Im, Sung-Won Kim, Suk Ho Bhang
Summary: Transplantation of optimized stem cell spheroids with increased cell compaction has shown enhanced therapeutic angiogenesis in ischemic tissues. This study successfully defined novel criteria for spheroid culture, potentially contributing to future stem cell therapies.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Daheui Choi, Alan M. Gonzalez-Suarez, Mihai G. Dumbrava, Michael Medlyn, Jose M. de Hoyos-Vega, Frank Cichocki, Jeffrey S. Miller, Li Ding, Mojun Zhu, Gulnaz Stybayeva, Alexandre Gaspar-Maia, Daniel D. Billadeau, Wen Wee Ma, Alexander Revzin
Summary: The study describes the development of microfluidic devices for testing therapies using a limited amount of tissue or PDOs available from pancreatic cancer biopsies.It demonstrates that microfluidic PDOs are similar to the gold-standard Matrigel organoids and offers several advantages. The utility of microfluidic PDOs is proven by testing PDO responses to several chemotherapies and immunotherapies.
Article
Cell Biology
Shira Toledano, Adi D. Sabag, Neta Ilan, Tanya Liburkin-Dan, Ofra Kessler, Gera Neufeld
Summary: Inhibiting the expression of plexin-A2 strongly suppresses cell proliferation and tumor formation ability in glioblastoma-derived cells and primary human endothelial cells. This inhibition also leads to cell cycle arrest, changes in cytoskeletal organization, cell flattening, and increased expression of senescence-associated β-galactosidase. The pro-proliferative effects of plexin-A2 are mediated by FARP2 and FYN and the GAP domain located in the intracellular domain of plexin-A2.
CELL DEATH & DISEASE
(2023)
Article
Health Care Sciences & Services
Alberto Izzotti, Gabriela Coronel Vargas, Alessandra Pulliero, Simona Coco, Irene Vanni, Cristina Colarossi, Giuseppina Blanco, Antonella Agodi, Martina Barchitta, Andrea Maugeri, Gea Oliveri Conti, Margherita Ferrante, Salvatore Sciacca
Summary: The study analyzed the interplay between miRNA expression and oncogene mutation, finding that miRNA analysis was more informative for predicting patient survival compared to mutation analysis.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Review
Oncology
Pablo Alvarez Ballesteros, Jesus Chamorro, Maria San Roman-Gil, Javier Pozas, Victoria Gomez Dos Santos, Alvaro Ruiz Granados, Enrique Grande, Teresa Alonso-Gordoa, Javier Molina-Cerrillo
Summary: Renal cell carcinoma is characterized by high vascularization and dense immune cell infiltration, with treatment strategies including angiogenesis inhibition and immune checkpoint inhibitors. Clear cell renal cell carcinoma, the most common subtype, has seen significant advancements in treatment strategies.
Article
Oncology
Wenqun Zhong, Zebin Xiao, Zhiyuan Qin, Jingbo Yang, Yi Wen, Ziyan Yu, Yumei Li, Neil C. Sheppard, Serge Y. Fuchs, Xiaowei Xu, Meenhard Herlyn, Carl H. June, Ellen Pure, Wei Guo
Summary: Chimeric antigen receptor (CAR) T-cell therapy has been successful in treating hematologic malignancies, but has limited efficacy against solid tumors. This study found that solid tumors release small extracellular vesicles (sEV) containing tumor antigens and the immune checkpoint protein PD-L1. These sEVs inhibit the proliferation, migration, and function of CAR T cells, acting as an active defense mechanism of solid tumors. Targeting tumor sEVs could optimize CAR T-cell therapy against solid tumors.
Article
Chemistry, Multidisciplinary
Camila M. L. Machado, Magdalena Skubal, Katja Haedicke, Fabio P. Silva, Evan P. Stater, Thais L. A. de O. Silva, Erico T. Costa, Cibele Masotti, Andreia H. Otake, Luciana N. S. Andrade, Mara de S. Junqueira, Hsiao-Ting Hsu, Sudeep Das, Benedict Mc Larney, Edwin C. Pratt, Yevgeniy Romin, Ning Fan, Katia Manova-Todorova, Martin Pomper, Jan Grimm
Summary: Cell membrane-derived particles (Mp) shed from tumor cells have the potential to be used as drug delivery vehicles for targeted and immunotherapeutic treatments. Prostate-specific membrane antigen (PSMA) is highly expressed in Mp and extracellular vesicles (EV) from prostate cancer. It has been discovered that PSMA can be transferred to the tumor microenvironment through Mp and alter cellular characteristics, resulting in increased secretion of angiogenic factors.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Orthopedics
Ruichen Ma, Jonathan Mandell, Feiqi Lu, Tanya Heim, Karen Schoedel, Anette Duensing, Rebecca J. Watters, Kurt R. Weiss
Summary: Patient-derived chondrosarcoma spheroids can be successfully produced and better recapitulate human chondrosarcoma than 2-D cultures both morphologically and molecularly. Chondrosarcoma spheroids provide an accurate model for testing novel treatments.
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
(2021)
Article
Urology & Nephrology
Laura Elst, Anne-Sophie Van Rompuy, Eduard Roussel, Lien Spans, Isabelle Vanden Bempt, Andrea Necchi, Jeffrey Ross, Joseph M. Jacob, Maria-Francesca Baietti, Eleonora Leucci, Maarten Albersen
Summary: Researchers have successfully established 11 patient-derived tumor xenograft (PDX) models of penile squamous cell carcinoma (pSCC), both HPV-positive and HPV-negative, and characterized them at the genomic and histological levels. These models may contribute to the development of novel treatments.
EUROPEAN UROLOGY FOCUS
(2022)
Article
Cell & Tissue Engineering
Jian Zhang, Yao Lu, Yangming Mao, Yue Yu, Tianyu Wu, Wei Zhao, Yeqian Zhu, Pengcheng Zhao, Fengxiang Zhang
Summary: Exosomes derived from MSCs activated with IFN-gamma exhibit enhanced cardioprotective effects by upregulating miR-21 and directly targeting BTG2 to promote angiogenesis and reduce apoptosis.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Oncology
Chiara Battistini, Ugo Cavallaro
Summary: This review summarizes the various types of in vitro patient-derived models of ovarian cancer (OC) and their ability to represent specific aspects of OC biology and provide personalized treatment tools.
Article
Surgery
Jinglin Zhu, Fangning Zhao, Yimeng Chai, Xinyu Jia, Facheng Li
Summary: In this study, the effectiveness of dedifferentiated fat cells (DFATs) and adipose-derived stem cells (ASCs) in enhancing fat graft survival was compared. The results showed that DFATs had a slight advantage over ASCs in terms of adipogenesis and angiogenesis. Both cell types significantly improved graft volume retention. DFATs group had marginally better retention rates, while the ASC group showed slightly enhanced graft tissue structure. These findings provide valuable insights for plastic surgeons aiming to elevate fat graft survival rates.
AESTHETIC SURGERY JOURNAL
(2023)
Article
Oncology
Ilaria Di Mauro, Damien Ambrosetti, Louis Vignot, Jean-Francois Roussel, Berengere Dadone-Montaudie, Annie-Claude Peyron, Herve Quintens, Matthieu Durand, Jean Amiel, Florence Pedeutour
GENES CHROMOSOMES & CANCER
(2020)
Article
Neurosciences
Nirvana Sadaghianloo, Julie Contenti, Serge Declemy, Damien Ambrosetti, Masa Zdralevic, Mounia Tannour-Louet, Lucilla Fabbri, Gilles Pages, Frederic Bost, Reda Hassen-Khodja, Jacques Pouyssegur, Elixene Jean-Baptiste, Alan Dardik, Nathalie M. Mazure
Summary: Patients with end-stage renal failure need AVFs for dialysis, but these AVFs often fail due to excessive venous thickness. This study found that venous cells adapt their metabolism depending on oxygen concentration, and targeting the hypoxic response pathway with drugs can help decrease excessive venous thickness. In a mouse model, inhibiting HIFs decreased neointimal hyperplasia, suggesting that drugs targeting HIFs could prevent AVF failure.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Biology
Maeva Dufies, Annelies Verbiest, Lindsay S. Cooley, Papa Diogop Ndiaye, Xingkang He, Nicolas Nottet, Wilfried Souleyreau, Anais Hagege, Stephanie Torrino, Julien Parola, Sandy Giuliano, Delphine Borchiellini, Renaud Schiappa, Baharia Mograbi, Jessica Zucman-Rossi, Karim Bensalah, Alain Ravaud, Patrick Auberger, Andreas Bikfalvi, Emmanuel Chamorey, Nathalie Rioux-Leclercq, Nathalie M. Mazure, Benoit Beuselinck, Yihai Cao, Jean Christophe Bernhard, Damien Ambrosetti, Gilles Pages
Summary: Dufies et al. found high Plk1 expression levels in aggressive clear cell renal cell carcinoma and discovered that Plk1 is transcriptionally upregulated in a manner dependent on HIF-2. They also found that high Plk1 expression is correlated to a poor prognosis and resistance to tyrosine kinase inhibitor against VEGF receptor, suggesting a critical role for hypoxia/HIF-2-induced Plk1 in disease progression.
COMMUNICATIONS BIOLOGY
(2021)
Article
Urology & Nephrology
Damien Ambrosetti, Michael Coutts, Charlotte Paoli, Matthieu Durand, Delphine Borchiellini, Christopher Montemagno, Olivia Rastoin, Arnaud Borderie, Renaud Grepin, Nathalie Rioux-Leclercq, Jean-Christophe Bernhard, Gilles Pages, Maeva Dufies
Summary: The study found that VEGFR-TKI significantly increased the number of CAFs in tumors, which were associated with shorter disease-free and overall survival in patients. The presence of CAFs was also correlated with lymphangiogenesis and lymph node metastasis, and CAFs promoted cell migration while reducing the cytotoxic effect of VEGFR-TKI on tumor cells.
Article
Oncology
Ilaria Di Mauro, Berengere Dadone-Montaudie, Mathilde Sibony, Damien Ambrosetti, Vincent Molinie, Myriam Decaussin-Petrucci, Vincent Bland, Claire Arbaud, Beatrice Cenciu, Francois Arbib, Pierre-Alexandre Just, Jonathan Derman, Nathalie Rioux-Leclercq, Florence Pedeutour
Summary: Accurately diagnosing Xp11-translocation renal cell carcinoma (RCC) in adults can be challenging, with TFE3 fusion generally occurring with a partner gene on another chromosome but occasionally with neighboring gene RBM10. Despite the difficulty, it is crucial to molecularly detect RBM10-TFE3 fusion as it is not exceptional in adult cases and may require targeted RNA sequencing for confirmation. Additionally, investigating RCC with type-2 papillary features and 17q gain by RNASeq could be a valuable avenue for further research.
GENES CHROMOSOMES & CANCER
(2021)
Article
Urology & Nephrology
Francois-Xavier Deledalle, Damien Ambrosetti, Mathieu Durand, Floriane Michel, Michael Baboudjian, Bastien Gondran-Tellier, Francois Lannes, Laurent Daniel, Marc Andre, Pierre-Olivier Fais, Pierre-Henri Savoie, Xavier Durand, Dominique Rossi, Gilles Karsenty, Cyrille Bastide, Eric Lechevallier, Romain Boissier
Summary: This study reported on the outcomes and feasibility of active surveillance of biopsy-proven renal oncocytomas. The results showed that active surveillance was oncologically safe with high patient adherence, low tumor growth rate, and high biopsy efficacy. No predictive factors for tumor growth could be identified.
Article
Dermatology
Christophe Perrin, Damien Ambrosetti
Summary: This study characterizes the features and diagnostic criteria of pleomorphic onychomatricoma, a rare condition that mimics malignant neoplasms.
ACTA DERMATO-VENEREOLOGICA
(2022)
Article
Biochemistry & Molecular Biology
Lindsay S. Cooley, Justine Rudewicz, Wilfried Souleyreau, Andrea Emanuelli, Arturo Alvarez-Arenas, Kim Clarke, Francesco Falciani, Maeva Dufies, Diether Lambrechts, Elodie Modave, Domitille Chalopin-Fillot, Raphael Pineau, Damien Ambrosetti, Jean-Christophe Bernhard, Alain Ravaud, Sylvie Negrier, Jean-Marc Ferrero, Gilles Pages, Sebastien Benzekry, Macha Nikolski, Andreas Bikfalvi
Summary: By serially passaging mouse renal cancer cells in vivo, researchers generated multiple cell lines with varying aggressiveness and identified distinct molecular markers and gene processes associated with different stages of tumor progression using transcriptome, genome and methylome analyses. Specific biomarkers such as SAA2 and CFB were identified as soluble prognostic and predictive markers of therapeutic response, with machine learning and mathematical modeling highlighting their significant impact on distant metastasis-free survival. A computational model predicting tumor progression and relapse was developed and validated, providing important translational implications for RCC therapy.
Article
Cell Biology
Solene-Florence Kammerer-Jacquet, Camille Gandon, Frederic Dugay, Brigitte Laguerre, Benoit Peyronnet, Romain Mathieu, Gregory Verhoest, Karim Bensalah, Xavier Leroy, Sebastien Aubert, Catherine Vermaut, Fabienne Escande, Virginie Verkarre, Eva Comperat, Damien Ambrosetti, Florence Pedeutour, Marc-Antoine Belaud-Rotureau, Nathalie Rioux-Leclercq
Summary: This study reports the clinical, histological, immunohistochemical, and genetic features of nine cases of renal cell carcinoma with TFEB amplification. The diagnosis of this rare molecular subgroup is confirmed by FISH analysis, and CGH analysis reveals complex genomic alterations. The prognosis is poor, and combination therapy might be beneficial for these aggressive tumors.
Letter
Cell Biology
Christophe Perrin, Giorgio Toni, Michael Coutts, Damien Ambrosetti
Review
Biochemistry & Molecular Biology
Audrey Simonaggio, Damien Ambrosetti, Virginie Verkarre, Marie Auvray, Stephane Oudard, Yann-Alexandre Vano
Summary: MiTF/TFE translocation renal cell carcinoma (tRCC) is a rare and aggressive subtype of RCC, which is more prevalent in the pediatric population and represents 4% of all RCCs in adults. The diagnosis of tRCC involves translocations of genes such as TFE3, TFEB or MITF. TFE break-apart fluorescent in situ hybridization (FISH) is considered the gold standard for diagnosis. tRCC exhibits heterogeneous clinical behaviors and is more aggressive in adults. Effective treatment options for metastatic tRCC are limited, highlighting the need for better therapeutic approaches. Comprehensive genetic sequencing analyses have provided insights into the biological heterogeneity of tRCC, aiding in the development of more effective treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Urology & Nephrology
Astrid Boulenger de Hauteclocque, Loic Ferrer, Damien Ambrosetti, Solene Ricard, Pierre Bigot, Karim Bensalah, Francois Henon, Nicolas Doumerc, Arnaud Mejean, Virginie Verkarre, Charles Dariane, Stephane Larre, Cecile Champy, Alexandre de La Taille, Franck Bruyere, Morgan Roupret, Philippe Paparel, Stephane Droupy, Alexis Fontenil, Jean-Jacques Patard, Xavier Durand, Thibaut Waeckel, Herve Lang, Cedric Lebacle, Laurent Guy, Geraldine Pignot, Matthieu Durand, Jean-Alexandre Long, Thomas Charles, Evanguelos Xylinas, Romain Boissier, Mokrane Yacoub, Thierry Colin, Jean-Christophe Bernhard
Summary: This study assessed the impact of pathological upstaging in renal cell carcinoma patients and evaluated the oncological safety of different surgical approaches. A machine-learning model was developed to predict upstaging. The findings showed that machine-learning technology can be useful in evaluating and predicting upstaging in renal cell carcinoma. Partial nephrectomy did not compromise oncological outcomes in incidental upstaging, even for large tumors.
Review
Urology & Nephrology
Michael Baboudjian, Daniel Moser, Takafumi Yanagisawa, Bastien Gondran-Tellier, Eva M. Comperat, Damien Ambrosetti, Laurent Daniel, Cyrille Bastide, Shahrokh F. Shariat, Eric Lechevallier, Pietro Diana, Alberto Breda, Benjamin Pradere, Romain Boissier
Summary: Active surveillance of biopsy-proven renal oncocytomas shows a low tumor growth rate, with oncological safety and good patient compliance. AS may represent a promising alternative to immediate treatment. Further studies on the long-term outcomes of AS are needed.
EUROPEAN UROLOGY OPEN SCIENCE
(2022)
Proceedings Paper
Cardiac & Cardiovascular Systems
Rudan Xiao, Eric Debreuve, Damien Ambrosetti, Xavier Descombes
Summary: This study investigates the importance of vascular network structure in RCC diagnosis, and builds a dataset and features to demonstrate the role of vascular networks in RCC histopathological images.
MEDICAL IMAGE COMPUTING AND COMPUTER ASSISTED INTERVENTION - MICCAI 2021, PT VI
(2021)
Article
Medicine, Research & Experimental
Anais Hagege, Damien Ambrosetti, Julien Boyer, Alexandre Bozec, Jerome Doyen, Emmanuel Chamorey, Xingkang He, Isabelle Bourget, Julie Rousset, Esma Saada, Olivia Rastoin, Julien Parola, Frederic Luciano, Yihai Cao, Gilles Pages, Maeva Dufies
Summary: High expression of Plk1 is associated with poor prognosis in HNSCC, while onvansertib shows potential therapeutic effects by inducing apoptosis. Combination of onvansertib with cisplatin and/or radiotherapy synergistically induces tumor cell death.