Article
Agriculture, Dairy & Animal Science
Yang Li, Yan Gao, Na Ling, Yizhong Shen, Danfeng Zhang, Dexin Ou, Xiyan Zhang, Rui Jiao, Changqing Zhu, Yingwang Ye
Summary: The study presented a comet structure combining SEA and surface-enhanced Raman spectroscopy for rapid and sensitive detection of foodborne Listeria monocytogenes. The method exhibited efficient amplification and desirable specificity, allowing for the detection of L. monocytogenes in cheese and providing a promising new approach for ensuring the microbiological safety of dairy products.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Chemistry, Analytical
Linlin Zhuang, Jiansen Gong, Ming Ma, Yongxin Ji, Peilong Tian, Xiuming Mei, Ning Gu, Yu Zhang
Summary: The ESEA-LFIA method developed in this study is a rapid and sensitive detection technology for the novel coronavirus, capable of completing the test within one hour, with good sensitivity and specificity for clinically relevant sequences.
Article
Microbiology
Etai Boichis, Nadejda Sigal, Ilya Borovok, Anat A. Herskovits
Summary: This study identified a conserved locus containing XRE regulators, a metzincin protease, and a lipoprotein structurally similar to a TIMP-family metzincin inhibitor, which play a crucial role in Listeria monocytogenes life cycle. The XRE regulators act as a regulatory switch that influences the expression of metzincin and TIMP-like genes, affecting Lm morphology and virion release. These proteins also modulate the cell wall structure and may hold promise in combating antibiotic resistant bacterial pathogens.
Article
Food Science & Technology
Xiaozhou Li, Xinnuo Wang, Lianyan Wang, Tianyue Yang, Deli Wang
Summary: A rapid detection method for the duplex detection of two beta-lactam antibiotics in milk powder using LF combined with SERS was reported. The method showed good regression fitting and a low limit of detection, indicating its potential for real-time multiplex and quantitative analysis of antibiotics in dairy products.
FOOD ANALYTICAL METHODS
(2021)
Article
Virology
Rasmus Riemer Jakobsen, Jimmy T. Trinh, Louise Bomholtz, Signe Kristine Brok-Lauridsen, Alexander Sulakvelidze, Dennis Sandris Nielsen
Summary: In this study, the effect of a bacteriophage cocktail (tentatively designated as the Foodborne Outbreak Pill (FOP)) on Listeria monocytogenes levels in simulated gastrointestinal models was investigated. The FOP was found to be robust in inhibiting the growth of L. monocytogenes, similar to antibiotic treatment, without affecting the commensal bacteria. Furthermore, the FOP showed better efficacy in protecting Caco-2 cells from invasion by L. monocytogenes compared to the antibiotic.
Article
Virology
Yaxiong Song, Tracey L. Peters, Daniel W. Bryan, Lauren K. Hudson, Thomas G. Denes
Summary: Listeria monocytogenes serotype 4b strains are commonly found in food processing environments and are often isolated in clinical settings. Bacteriophages, natural viral predators of bacteria, were studied for their ability to target and inhibit the growth of serotype 4b strains. Among 120 wild Listeria phages, nine were chosen for their lytic activity against a model serotype 4b strain, and they were divided into two groups based on their characteristics. Further studies on these phages showed inhibitory effects on the growth of serotype 4b strains, providing potential for biocontrol in food processing environments.
Article
Chemistry, Analytical
So-Young Lee, Unji Kim, Younggyu Kim, Seung Jae Lee, Eun Young Park, Se-Wook Oh
Summary: In this study, a highly sensitive detection method for Listeria monocytogenes was developed using TEPA-functionalized magnetic nanoparticles and a LAMP-CRISPR/Cas12a-based biosensor. The LAMP assay and CRISPR/Cas12a system parameters were optimized, along with the capture parameters for TEPA-functionalized magnetic nanoparticles, resulting in efficient detection of Listeria monocytogenes. The sensitivity of this method was 10 times higher than that of conventional methods, making it a significant advancement in pathogenic bacteria detection for food safety and public health.
ANALYTICA CHIMICA ACTA
(2023)
Article
Veterinary Sciences
Tianhao Li, Xuehui Zhao, Xuejian Wang, Zijian Wang, Changqing Tian, Wenjing Shi, Yumei Qi, Huilin Wei, Chen Song, Huiwen Xue, Huitian Gou
Summary: Studying the relationship between Listeria monocytogenes and natural phages, we discovered that the phage LP8 has therapeutic effects against listeriosis, demonstrating the feasibility of combined therapy to reduce antibiotic usage.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Biochemical Research Methods
Matheus Bernardes Torres Fogaca, Arun K. Bhunia, Leonardo Lopes-Luz, Eduardo Pimenta Ribeiro Pontes de Almeida, Jose Daniel Goncalves Vieira, Samira Buhrer-Sekula
Summary: The use of lateral flow immunoassay (LFIA) and nucleic acid-based lateral flow immunoassay (NALFIA) can improve the sensitivity and specificity of Listeria monocytogenes detection, accelerating the testing process. By improving the ligands and configuration of assay kits, the detection sensitivity and automated interpretation of results in LFIA can be enhanced. Integration of surface-enhanced Raman spectroscopy and immunomagnetic separation technologies can significantly improve the sensitivity of LFIA for rapid food safety monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Jack Burnett, Sophie Tongyu Wu, Maxwell Voorn, Christopher Jordan, Clyde S. Manuel, Manpreet Singh, Haley F. Oliver
Summary: Our study on Listeria monocytogenes prevalence and persistence in retail produce environments revealed that some departments harbor persistent isolates of L. monocytogenes. Through re-training and deep cleaning, we were able to reduce the overall prevalence of L. monocytogenes, although only a few stores significantly reduced their prevalence. Factors such as the presence of foam squeegees, damaged equipment, or plumbing leaks predicted the effectiveness of the intervention.
Article
Microbiology
Zuhua Yu, Yingying Zhao, Ke Ding, Lei He, Chengshui Liao, Jing Li, Songbiao Chen, Ke Shang, Jian Chen, Chuan Yu, Chunjie Zhang, Yinju Li, Shaohui Wang, Yanyan Jia
Summary: This study demonstrated that Listeria monocytogenes (LM) exhibits antitumor effects by inducing autophagy, and the combination of LM and the autophagy inhibitor chloroquine (CQ) enhances the antitumor activity of LM both in vitro and in vivo.
Article
Food Science & Technology
Yingying Li, Haihuan Xie, Jin Wang, Xiangmei Li, Zhili Xiao, Zhenlin Xu, Hongtao Lei, Xing Shen
Summary: This study established a rapid, convenient and sensitive lateral flow immunochromatography (LFIA) based on Au nanoparticles (AuNPs) for the detection of furosemide in slimming health foods. The developed AuNPs-LFIA showed high consistency with liquid chromatography with tandem mass spectrometry (LC-MS/MS), demonstrating its accuracy and reliability in detecting furosemide.
Article
Biotechnology & Applied Microbiology
Cristina Rodriguez-Melcon, Victor Serrano-Galan, Rosa Capita, Carlos Alonso-Calleja
Summary: The aim of this research was to investigate the effect of subinhibitory concentrations of disinfectants on the resistance of Listeria monocytogenes to tetracycline. The results showed that exposure to subinhibitory doses of the disinfectants could lead to increased resistance to tetracycline. The use of disinfectants at subinhibitory concentrations should be avoided to prevent the development of resistance.
Article
Biophysics
Xinyu Liu, Xiaoming Su, Mingyang Chen, Yangcenzi Xie, Ming Li
Summary: Rapid early diagnosis of Alzheimer's disease is crucial, but challenging. In this study, a self-calibrating biosensor based on SERS-LFIA technique is developed for accurate AD diagnosis. It has high sensitivity, excellent anti-interference capability, lowcost and easy operation.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Chemistry, Analytical
Kseniya V. Serebrennikova, Nadezhda A. Byzova, Anatoly V. Zherdev, Nikolai G. Khlebtsov, Boris N. Khlebtsov, Sergey F. Biketov, Boris B. Dzantiev
Summary: The study developed and characterized a modified LFIA for a specific antigen of COVID-19, which includes the use of gold nanoparticles with SERS nanotags to achieve a low detection limit in a short assay time. The results demonstrate the promise of using SERS effects in membrane immuno-analytical systems.