Article
Veterinary Sciences
Rebecca P. Wilkes
Summary: Although still mainly used for research purposes, NGS has been adopted by some veterinary diagnostic laboratories. Metagenomics approaches offer the widest detection range for infectious disease diagnostics, but at a higher cost. tNGS maintains the ability to detect a broad range of pathogens at a lower cost compared to mNGS, making it a viable option for syndromic testing in veterinary diagnostic laboratories.
VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE
(2023)
Article
Ophthalmology
Roos A. W. Wennink, Joke H. de Boer, Sanne Hiddingh, Anne-Mieke J. W. Haasnoot, Viera Kalinina Ayuso, Talitha de Hoop, Jessica van Setten, Eric Spierings, Jonas J. W. Kuiper
Summary: This study revealed novel shared HLA associations among clinically distinct phenotypes of pediatric uveitis and highlighted genetic predisposition affecting the antigen presentation pathway.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Medicine, General & Internal
Yifan Guo, Henan Li, Hongbin Chen, Zhenzhong Li, Wenchao Ding, Jun Wang, Yuyao Yin, Longyang Jin, Shijun Sun, Chendi Jing, Hui Wang
Summary: The study established a pipeline for simultaneous detection of pathogens and cancer using Illumina sequencing of lung biopsy tissue, achieving high sensitivity and specificity in infection and cancer diagnosis. The Nanopore platform showed lower sensitivity in pathogen detection compared to Illumina.
Article
Multidisciplinary Sciences
Kemal Avican, Jehad Aldandooh, Matteo Togninalli, A. K. M. Firoj Mahmud, Jing Tang, Karsten M. Borgwardt, Mikael Rhen, Maria Fallman
Summary: In this study, Avican et al. presented global transcriptomes for 32 bacterial pathogens grown under 11 stress conditions, identifying common and unique regulatory responses, as well as processes participating in different stress responses. Bacterial stress responses are potential targets for new antimicrobials.
NATURE COMMUNICATIONS
(2021)
Article
Ophthalmology
Junfeng Zhu, Honghe Xia, Ruqing Tang, Tsz Kin Ng, Fen Yao, Xulong Liao, Qi Zhang, Xixuan Ke, Tingkun Shi, Haoyu Chen
Summary: This study investigated the use of metagenomic next-generation sequencing (mNGS) in identifying pathogens in endophthalmitis. The results showed that mNGS had a higher positive rate than traditional culture methods and could provide information on antibiotic resistance and visual prognosis.
RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES
(2022)
Article
Immunology
Jiachun Su, Xu Han, Xiaogang Xu, Wenchao Ding, Ming Li, Weiqin Wang, Mi Tian, Xiyuan Chen, Binbin Xu, Zhongqing Chen, Jinyi Yuan, Xiaohua Qin, Dongfang Lin, Ruilan Wang, Ye Gong, Liping Pan, Jun Wang, Minggui Wang
Summary: The clinical value of using metagenomic next-generation sequencing (mNGS) to detect tumors in patients with suspected infections has been demonstrated.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Doniddemberel Altantogtokh, Abigail A. Lilak, Ratree Takhampunya, Jira Sakolvaree, Nitima Chanarat, Graham Matulis, Betty Katherine Poole-Smith, Bazartseren Boldbaatar, Silas Davidson, Jeffrey Hertz, Buyandelger Bolorchimeg, Nyamdorj Tsogbadrakh, Jodi M. Fiorenzano, Erica J. Lindroth, Michael E. von Fricken
Summary: Tick-borne diseases are a major public health concern in Mongolia, especially for nomadic pastoralists. This study identified several tick-borne pathogens in Dermacentor ticks, with Rickettsia having the highest infection rate. Livestock play a significant role in disease maintenance. The detection of Anaplasma, Bartonella, and Rickettsia highlights the increased risk for infection throughout Mongolia. Further research is needed to characterize tick-borne pathogens in other endemic tick species.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Entomology
Sheane Andrea V. Hernandez, Saubel Ezrael A. Salamat, Remil L. Galay
Summary: Analysis of the tick microbiome provides insights into tick-symbiont interactions and potential pathogens. This study characterized the bacterial community of the tropical cattle tick Rhipicephalus microplus in the Philippines and identified known cattle pathogens. The diversity of bacterial species in the tick samples varied among provinces. This is the first study on metagenomic analysis of cattle ticks in the Philippines, offering valuable information for tick control and prevention of tick-borne diseases.
EXPERIMENTAL AND APPLIED ACAROLOGY
(2023)
Article
Microbiology
Jobin J. Kattoor, Emma Nikolai, Barbara Qurollo, Rebecca P. Wilkes
Summary: The study developed and validated a targeted next-generation sequencing (NGS) assay for vector-borne pathogens. The assay showed good analytical specificity and diagnostic sensitivity, making it a viable method for detecting vector-borne pathogens in canine whole blood samples.
Article
Multidisciplinary Sciences
Mahmoud Gad, Mariam E. Fawzy, Ahmad Z. Al-Herrawy, Sayeda M. Abdo, Noura Nabet, Anyi Hu
Summary: This study utilized high-throughput sequencing to investigate the diversity and composition of Apicomplexa community in riverine environments. The results showed habitat heterogeneity and revealed differences in dominant genera between inlet and outlet samples. Additionally, surface water samples exhibited variations in the relative abundance of certain genera.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Dengfeng Wu, Wei Wang, Qiufen Xun, Hongluan Wang, Jiarong Liu, Ziqing Zhong, Chao Ouyang, Qing Yang
Summary: This study evaluated the diagnostic performance of metagenomic next-generation sequencing (mNGS) in patients with suspected pulmonary infection and found that mNGS can greatly enhance the accuracy and detection rate of pathogens. Comorbidities and types of pathogens should be taken into consideration when interpreting the results of mNGS.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Shoji Takenaka, Naoki Edanami, Yasutaka Komatsu, Ryoko Nagata, Traithawit Naksagoon, Maki Sotozono, Takako Ida, Yuichiro Noiri
Summary: This study analyzed the microbiome of root caries lesions in patients with severe periodontitis and found compositionally distinct microbiota depending on the location of the lesions. Periodontal pathogens were identified in root caries lesions that extended beyond the gingival margin, providing new insights into the microbial etiology of root caries.
Article
Clinical Neurology
Guanzhong Wang, Jiang Long, Yong Zhuang, Xue Leng, Yaqing Zhang, Libangxi Liu, Jiawei Fu, Yu Chen, Changqing Li, Yue Zhou, Bo Huang, Chencheng Feng
Summary: Metagenomic next-generation sequencing (mNGS) is an accurate and efficient method for identifying pathogens in spinal infections (SI). It has higher sensitivity and lower time cost compared to bacterial culture. The application of mNGS in SI diagnosis is significant and should be further explored.
Article
Microbiology
Yunqi Zhu, Wenxuan Zhao, Xihong Yang, Yuanyuan Zhang, Xiaoling Lin, Xing Weng, Yali Wang, Cong Cheng, Yun Chi, Hongxia Wei, Zhihang Peng, Zhiliang Hu
Summary: This study found that mNGS has a high sensitivity for detecting common opportunistic CNS pathogens in HIV-infected patients, although its performance in CNS tuberculosis is unsatisfactory. EBV and CMV are commonly detected by CSF mNGS, but the threshold for clinically significant detection still needs to be defined.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Immunology
Farokh Dotiwala, Arun K. Upadhyay
Summary: Inducing mucosal immunity at the sites of pathogen entry has the potential to prevent infection, and adenoviral vectors have improved mucosal vaccine research. Mucosal vaccines can induce local immune responses and also produce systemic immune responses.
Article
Infectious Diseases
Pavle Banovic, Adrian Alberto Diaz-Sanchez, Angelique Foucault-Simonin, Lourdes Mateos-Hernandez, Alejandra Wu-Chuang, Clemence Galon, Verica Simin, Dragana Mijatovic, Ivana Bogdan, Belkis Corona-Gonzalez, Liani Coronado Baez, Joanna Kulisz, Aneta Wozniak, Zbigniew Zajac, Dasiel Obregon, Sara Moutailler, Alejandro Cabezas-Cruz
Summary: The incidence of tick-borne diseases has been increasing worldwide, resulting in a greater impact on human health. This study reported nine cases of atypical tick-borne diseases in Serbia, with five confirmed cases of Spotted Fever group (SFGR) infection. Clinical symptoms included persistent circular redness, eschar, lymph node enlargement, and pain at the site of the lesion. The detection of Rickettsia helvetica and antibodies reactive to Rickettsia conorii antigen suggests exposure to SFGR.
INFECTION GENETICS AND EVOLUTION
(2023)
Article
Parasitology
Branka Bilbija, Caecilia Spitzweg, Ivo Papousek, Uwe Fritz, Gabor Foeldvari, Martin Mullett, Flora Ihlow, Hein Sprong, Kristina Civanova Krizova, Nikolay Anisimov, Oxana A. Belova, Sarah I. Bonnet, Elizabeth Bychkova, Aleksandra Czulowska, Georg G. Duscher, Manoj Fonville, Olaf Kahl, Grzegorz Karbowiak, Ivan S. Kholodilov, Dorota Kiewra, Stjepan Krcmar, Gulzina Kumisbek, Natalya Livanova, Igor Majlath, Maria Teresa Manfredi, Andrei D. Mihalca, Guadalupe Miro, Sara Moutailler, Igor V. Nebogatkin, Snezana Tomanovic, Zati Vatansever, Marya Yakovich, Sergio Zanzani, Pavel Siroky
Summary: The population structure of the ornate dog tick in Eastern Europe exhibits an east-west gradient, which may be influenced by geographic factors and genetic evolution. This finding is crucial for assessing disease transmission risks and predicting future spread.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2023)
Article
Parasitology
Camille Lacroux, Sarah Bonnet, Emmanuelle Pouydebat, Marie Buysse, Nil Rahola, Sabine Rakotobe, John-Paul Okimat, Rachid Koual, Edward Asalu, Sabrina Krief, Olivier Duron
Summary: This study investigated ticks and tick-borne pathogens in a forested habitat of a community of 100 chimpanzees living in Kibale National Park, Western Uganda. The presence and abundance of ticks were found to be influenced by environmental factors, and various tick-borne pathogens were detected. The results of this study are important for understanding the health status of chimpanzees and other wildlife.
PARASITES & VECTORS
(2023)
Article
Microbiology
Abdul Ghafar, Nick Davies, Mythili Tadepalli, Amanda Breidahl, Clare Death, Philip Haros, Yuting Li, Peter Dann, Alejandro Cabezas-Cruz, Sara Moutailler, Angelique Foucault-Simonin, Charles G. Gauci, John Stenos, Jasmin Hufschmid, Abdul Jabbar
Summary: Ticks of Australian wildlife carry a diverse array of microorganisms, including Apicomplexa, Rickettsia, Theileria, Bartonella, Coxiella-like, Hepatozoon, and Ehrlichia. The study highlights the need for continuous surveillance of tick-associated pathogens/microorganisms to minimize health threats to humans and animals.
Article
Veterinary Sciences
Roland Eric Yessinou, Cristina Daniela Cazan, Luciana Catalina Panait, Eyabana Mollong, Abel S. S. Biguezoton, Sarah Irene Bonnet, Souaibou Farougou, Martin H. H. Groschup, Andrei Daniel Mihalca
Summary: This study investigated the presence of zoonotic pathogens in ticks collected from cattle in Benin and Togo, revealing the presence of Bartonella spp. and Rickettsia spp. This information is important for raising awareness among physicians and veterinarians, monitoring the spread of these pathogens, and implementing necessary measures to control the transmission of tick-borne diseases.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Virology
Claude Saegerman, Marie-France Humblet, Marc Leandri, Gaelle Gonzalez, Paul Heyman, Hein Sprong, Monique L'Hostis, Sara Moutailler, Sarah I. Bonnet, Nadia Haddad, Nathalie Boulanger, Stephen Leib, Thierry Hoch, Etienne Thiry, Laure Bournez, Jana Kerlik, Aurelie Velay, Solveig Jore, Elsa Jourdain, Emmanuelle Gilot-Fromont, Katharina Brugger, Julia Geller, Marie Studahl, Natasa Knap, Tatjana Avsic-Zupanc, Daniel P. Ruzek, Tizza Zomer, Rene Bodker, Thomas F. H. Berger, Sandra Martin-Latil, Nick De Regge, Alice A. Raffetin, Sandrine Lacour, Matthias Klein, Tinne Lernout, Elsa Quillery, Zdenek Hubalek, Francisco Ruiz-Fons, Agustin Estrada-Pena, Philippe Fravalo, Pauline Kooh, Florence Etore, Celine M. Gossner, Bethan Purse
Summary: Tick-borne encephalitis (TBE) is an endemic viral disease in Eurasia, primarily transmitted to humans through ticks and occasionally through unpasteurized milk products. The European Centre for Disease Prevention and Control has reported an increase in TBE incidence over the years, as well as its emergence in new areas. Through expert knowledge elicitation, we identified nine key drivers for TBE emergence and increased incidence, including changes in human behavior, eating habits, landscape, humidity, difficulty in controlling reservoirs and vectors, temperature, wildlife compartments, increase in autochthonous wild mammals, and distribution of tick species vectors.
Article
Microbiology
Baptiste Defaye, Sara Moutailler, Benjamin Vollot, Clemence Galon, Gaelle Gonzalez, Rayane Amaral Moraes, Antoine-Simon Leoncini, Amalia Rataud, Gilles Le Guillou, Vanina Pasqualini, Yann Quilichini
Summary: In this study, blood samples and hematophagous ectoparasites were collected from migratory and sedentary bird populations in Corsican wetlands. Five pathogens, including Trypanosoma sp. in louse flies and Anaplasma phagocytophilum, Erhlichia chaffeensis, and Rickettsia helvetica in ticks, were detected. Additionally, Ehrlichia chaffeensis and the West Nile virus were detected in bird blood samples in Corsica. This is the first report of these tick, louse fly, and pathogen species isolated on the bird population in Corsica, highlighting the importance of bird populations in the presence of arthropod-borne pathogens in Corsican wetlands.
Editorial Material
Microbiology
Sarah I. Bonnet, Stephane Bertagnoli, Alessandra Falchi, Julie Figoni, Johanna Fite, Thierry Hoch, Elsa Quillery, Sara Moutailler, Alice Raffetin, Magalie Rene-Martellet, Gwenael Vourc'h, Laurence Vial
Summary: Current and future changes in the distribution of Hyalomma ticks, which are vectors of pathogens, are of concern. However, there is limited evidence to validate the transmission of specific pathogens by specific Hyalomma species. A bibliographical study found very few validated cases of pathogen transmission by Hyalomma ticks.
Article
Microbiology
Youmna M'ghirbi, Laurence Mousson, Sara Moutailler, Sylvie Lecollinet, Rayane Amaral, Cecile Beck, Hajer Aounallah, Meriem Amara, Ahmed Chabchoub, Adel Rhim, Anna-Bella Failloux, Ali Bouattour
Summary: Mosquito-borne diseases have a significant impact on humans and animals, especially with environmental changes. This study in Tunisia is the first to report the detection of West Nile virus (WNV), Usutu virus (USUV), and Sindbis virus (SINV) in Cx. perexiguus mosquitoes. Moreover, the study found a significant circulation of WNV and USUV among horses, indicating the potential for future outbreaks. The integrated arbovirus surveillance system that includes entomological surveillance is important for early detection and control.
Article
Biochemistry & Molecular Biology
Apolline Maitre, Alejandra Wu-Chuang, Lourdes Mateos-Hernandez, Elianne Piloto-Sardinas, Angelique Foucault-Simonin, Vincent Cicculli, Sara Moutailler, Jean-Christophe Paoli, Alessandra Falchi, Dasiel Obregon, Alejandro Cabezas-Cruz
Summary: In this study, scientists used high-throughput pathogen detection and co-occurrence network analysis based on 16S rRNA sequencing to show that tick-borne Rickettsia play a significant role in tick bacterial microbiota. They contribute to the conservation of the 'core bacterial microbiota' and promote community stability.
Article
Microbiology
Zbigniew Zajac, Joanna Kulisz, Aneta Wozniak, Katarzyna Bartosik, Angelique Foucault-Simonin, Sara Moutailler, Alejandro Cabezas-Cruz
Summary: This study investigated the presence of tick species in the Western Carpathians, Poland and examined the impact of environmental factors on tick occurrence and activity, the prevalence of vectored pathogens, and tick hosts as reservoir organisms. The results showed that only two species of ticks were found in the study area, with temperature significantly affecting tick activity and the number of Ixodes ricinus nymphs varying with altitude. The main hosts for ticks were rodents, and the ticks collected from vegetation were often infected with various pathogens.
Article
Virology
Porcelli Stefania, Heckmann Aurelie, Lagree Anne-Claire, Galon Clemence, Moutailler Sara, Deshuillers Lucien Pierre
Summary: Ticks and tick-borne diseases are recognized as a critical One Health concern. This study assessed the susceptibility of C3H mice to tick-borne encephalitis virus (TBEV) infection and laid the groundwork for future research on co-infection models involving TBEV and Borrelia.