Article
Agriculture, Dairy & Animal Science
Bilal Ahmad Malla, Zunjar Baburao Dubal, Anukampa Kadwalia, Gazanfar Abass, Obli Rajendran Vinodh Kumar, Ashok Kumar, Kaushal Kishore Rajak, Ishfaq Maqbool, Aquil Mohmad, Vivekanandhan Rangaraju, Arfa Fayaz
Summary: Rapid and reliable diagnosis of diarrhoeal disease is important for distinguishing the etiological agents and choosing suitable treatment. This study reveals a seasonal pattern in the occurrence of rotavirus in diarrheic children, calves, and piglets from Bareilly, Uttar Pradesh, India. The positivity rate is higher in winter compared to autumn.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Virology
Moustafa Gibory, Tone Bruun, Elmira Flem, Jennifer Lynn Dembinski, Ildri Haltbakk, Ketil Stordal, Svein Arne Nordbo, Kirsti Jakobsen, Elisebet Haarr, Truls Michael Leegaard, Susanne Gjeruldsen Dudman
Summary: After the introduction of the rotavirus vaccine in Norway, there was no clear evidence of vaccine-induced virus strain replacement, with G1P[8] being the most common type both pre and post vaccine implementation. The study revealed that 21.4% of cases detected were vaccine strains, highlighting the importance of continuous genotype surveillance for assessing vaccine program effectiveness.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Food Science & Technology
Chenang Lyu, Feifeng Lu, Zhentao Shi, Dapeng Wang
Summary: The study developed an ISC-RT-qPCR assay for detecting encapsulated RVA in oysters with higher sensitivity and accuracy than RT-qPCR. When applied to oysters collected from four different farms, ISC-RT-qPCR showed a higher detection rate of RVA compared to RT-qPCR, indicating its potential as a better method for disease monitoring and control.
Article
Veterinary Sciences
Yushun Wang, Mincai Nie, Huidan Deng, Siyuan Lai, Yuancheng Zhou, Xiangan Sun, Ling Zhu, Zhiwen Xu
Summary: In this study, a rapid and specific diagnostic method for porcine rotavirus type A (PoRVA) was successfully developed. The method utilized specific primers and probes designed based on the PoRVA VP6 gene, and obtained detection results that were completely consistent with the TaqMan probe-based RT-qPCR method in clinical samples.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Jean-Claude Kabayiza, Staffan Nilsson, Maria Andersson
Summary: Rotavirus vaccination in Rwanda has reduced mortality and hospital admissions due to rotavirus diarrhea, but its effect on rotavirus infections and the impact of rotavirus genotypes are still unclear.
Article
Infectious Diseases
Nosheen Basharat, Asma Sadiq, Muhammad Dawood, Shahid Ali, Alam Khan, Rooh Ullah, Hayat Khan, Aamir Aziz, Hamid Ali, Aamer Ali Shah, Ijaz Ali, Jadoon Khan
Summary: This study aimed to detect the prevalence of Rotavirus A (RVA) in children in Pakistan after the introduction of rotavirus vaccines. Results showed a high prevalence of RVA in children with acute gastroenteritis (AGE), with no significant differences in gender and age among RVA infections. The seasons of winter, spring, and fall/autumn were statistically significant for RVA prevalence. Recommendations include implementing rotavirus vaccines, providing adequate nutrition and clean water supply for infants, and continuous surveillance to calculate the disease burden caused by RVA.
BMC INFECTIOUS DISEASES
(2021)
Article
Virology
Tirth Uprety, Chithra C. Sreenivasan, Ben M. Hause, Ganwu Li, Solomon O. Odemuyiwa, Stephan Locke, Jocelynn Morgan, Li Zeng, William F. Gilsenan, Nathan Slovis, Laurie Metcalfe, Craig N. Carter, Peter Timoney, David Horohov, Dan Wang, Erdal Erol, Emma Adam, Feng Li
Summary: The increase in severe watery to hemorrhagic diarrhea cases in neonatal foals in Central Kentucky in 2021 was linked to a novel equine rotavirus group B (ERVB) originating from ruminants. Although diagnostic investigations failed to detect ERVA, metagenomic sequencing revealed the presence of ERVB in affected foals, with similarities to ruminant-derived group B rotaviruses. This emergence of a ruminant-like group B rotavirus in foals highlights the importance of further investigation due to its impact on neonatal foals and the equine industry.
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Agriculture, Dairy & Animal Science
Gyu-Nam Park, SeEun Choe, Ra Mi Cha, Jihye Shin, Ki-Sun Kim, Byung-Hyun An, Song-Yi Kim, Bang-Hun Hyun, Dong-Jun An
Summary: The study reveals that G6P[5] genotype of bovine group A rotavirus is the main causative agent of diarrhea in Korean calves, replacing the previously dominant G8P[7] genotype.
Article
Virology
Wenwen Hou, Maodi Fan, Zhenbang Zhu, Xiangdong Li
Summary: A triplex real-time RT-PCR assay based on TaqMan probes was developed for differential detection of PEDV, PoRV, and PDCoV. The assay showed satisfactory specificity, sensitivity, repeatability, and reproducibility. In the clinical samples, the co-infections of PEDV and PoRV were frequent, while the prevalence of PDCoV was relatively low.
Article
Agriculture, Dairy & Animal Science
Gazanfar Abass, Zunjar Baburao Dubal, Kaushal K. Rajak, Balasaheb M. Kale, Abhay Raorane, Nitin Dudhe, Bilal Ahmad Malla, Dhananjay Desai, Dharmendra K. Sinha, Obli R. Vinodh Kumar, Yashpal Singh Malik
Summary: This study investigated the genotypes of RVA in pig populations in western and northern India. The results showed the circulation of G4P[6] and G9P[13] RVA strains in pigs, indicating evidence of inter-species transmission with human RVA strains.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Virology
Yi Tian, Zhiyong Gao, Weihong Li, Baiwei Liu, Yanwei Chen, Lei Jia, Hanqiu Yan, Quanyi Wang
Summary: The molecular epidemiology of RVA among adult outpatients with diarrhea in Beijing from 2011 to 2018 was analyzed. The study found that RVA is a major cause of adult diarrhea in Beijing, with continuous transmission and the G9P[8] genotype becoming dominant.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Environmental Sciences
Manupati Hemalatha, Uday Kiran, Santosh Kumar Kuncha, Harishankar Kopperi, C. G. Gokulan, S. Venkata Mohan, Rakesh K. Mishra
Summary: The SARS-CoV-2 pandemic has had devastating effects globally, with sewage surveillance proving useful in understanding viral spread. A study in Hyderabad, India showed that sewage treatment efficiently eliminates the virus, highlighting the potential of sewage-based surveillance in managing COVID-19 transmission.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Veterinary Sciences
Yangkun Liu, Xueying Han, Xinru Zhang, Jiaxing Liu, Lunguang Yao
Summary: Group A porcine rotavirus (PoRVA) is a significant pathogen causing acute enteritis in piglets worldwide. This study developed a novel and convenient droplet digital PCR (ddPCR) using the conserved region of the VP6 gene for PoRVA detection. The ddPCR exhibited 10 times greater sensitivity than TaqMan real-time quantitative PCR (qPCR), and was highly specific for PoRVA. Clinical sample testing showed a higher positivity rate of ddPCR (5.6%) compared to qPCR (4.4%). The newly developed ddPCR assay can be widely used for clinical diagnosis of PoRVA infections.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Environmental Sciences
Sabrina Guerin-Rechdaoui, Ariane Bize, Camille Levesque-Ninio, Alice Janvier, Carlyne Lacroix, Florence Le Brizoual, Jerome Barbier, Celine Roose Amsaleg, Sam Azimi, Vincent Rocher
Summary: This study investigated the stability of SARS-CoV-2 in wastewater treatment sludge and found that the RNA levels of SARS-CoV-2 remained stable or decreased rapidly during storage and thermophilic anaerobic digestion. The results suggest that thermophilic anaerobic digestion is an effective process for sludge sanitation.
ENVIRONMENTAL RESEARCH
(2022)
Article
Neurosciences
Sanaa Y. Shaaban, Yasmin G. El Gendy, Nayra S. Mehanna, Waled M. El-Senousy, Howaida S. A. El-Feki, Khaled Saad, Osama M. El-Asheer
NUTRITIONAL NEUROSCIENCE
(2018)
Article
Environmental Sciences
Waled M. El-Senousy, Adel Abdel-Moneim, Mahmoud Abdel-Latif, Mohamed H. EL-Hefnawy, Rehab G. Khalil
FOOD AND ENVIRONMENTAL VIROLOGY
(2018)
Article
Environmental Sciences
Waled Morsy El-Senousy, Sohair Imam Abou-Elela
FOOD AND ENVIRONMENTAL VIROLOGY
(2017)
Article
Medicine, General & Internal
Adel Abdel-Moneim, Waled M. El-Senousy, Mahmoud Abdel-Latif, Rehab G. Khalil
MEDICAL PRINCIPLES AND PRACTICE
(2018)
Article
Biochemistry & Molecular Biology
Azza Abdelmageed Matloub, Eman Zakaria Gomaa, Amira Ali Hassan, Marwa Mahmoud Elbatanony, Waled Morsy El-Senousy
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2020)
Article
Chemistry, Applied
A. S. Montaser, Mohamed Rehan, W. M. El-Senousy, Saad Zaghloul
CARBOHYDRATE POLYMERS
(2020)
Article
Environmental Sciences
Waled M. El-Senousy, Amel S. M. Abu Senna, Nabil A. Mohsen, Seham F. Hasan, Nagwa M. Sidkey
FOOD AND ENVIRONMENTAL VIROLOGY
(2020)
Article
Environmental Sciences
Waled M. El-Senousy, Maha Shalaby, Azza M. M. Deeb, Ibrahim I. Alhawary
FOOD AND ENVIRONMENTAL VIROLOGY
(2020)
Article
Environmental Sciences
Waled Morsy El-Senousy, Mohamed Shouman
Summary: The study in Egypt from 2005-2006 found no presence of HCoV-NL63 in clinical specimens of children and raw sewage samples, while other respiratory viruses like parainfluenza virus, respiratory syncytial virus, and non-enteric adenoviruses were detected in some clinical specimens. Enteric adenoviruses showed a higher prevalence in raw sewage samples, possibly due to direct transmission.
FOOD AND ENVIRONMENTAL VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rehab G. Khalil, Adel Abdel-Moneim, Amany A. Arafa, Gamal Allam, Waled M. El-Senousy, Doaa Mabrouk
Summary: This study examined the association of RV IgG with cytokine levels and dyslipidemia in pediatric T1D. The results suggested that anti-RV IgG may play a role in the pathogenesis and progression of T1D, and RV infections are implicated in dyslipidemia and inflammation status.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Environmental Sciences
Mohammed Kamal Rashed, Waled Morsy El-Senousy, ElSayed Tarek Abd ElSalam Sayed, Maha AlKhazindar
Summary: The objective of this study was to compare different types of viruses as viral indices for sewage and water samples. The results showed that infectious adenoviruses and infectious bacteriophage phiX174 virus performed better as viral indices in drinking water and treated sewage samples.
FOOD AND ENVIRONMENTAL VIROLOGY
(2022)
Article
Pediatrics
Amany A. Arafa, Adel Abdel-Moneim, Rehab G. Khalil, Waled M. El-Senousy, Mahmoud M. Kamel, Dalia Y. Kadry, Gamal Allam, Ahmed S. Abdel-Moneim
Summary: This study found a link between adenovirus infection and the development of type 1 diabetes in children. Adenovirus infection may be a contributing risk factor for T1D and plays a role in its induction.
Article
Biochemistry & Molecular Biology
Hatem A. Abuelizz, Ahmed H. Bakheit, Mohamed Marzouk, Waled M. El-Senousy, Mohamed M. Abdellatif, Gamal A. E. Mostafa, Rashad Al-Salahi
Summary: Globally, rotavirus is the leading cause of acute gastroenteritis in infants and toddlers, but there are currently no specific treatments for rotavirus infection. Immunization programs are being implemented worldwide to reduce the impact of rotavirus, but there are no approved antiviral drugs that can attack rotavirus in hosts. Benzoquinazoline derivatives synthesized in the laboratory showed good antiviral activity against various viruses. In this research, the effectiveness of benzoquinazoline derivatives against human rotavirus Wa strains was investigated in vitro. Compounds 1-3, 9, and 16 showed the highest activity, potentially inhibiting the outer capsid protein VP4.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
A. A. Alaneny, W. M. El-Senousy, A. Z. Al-Herrawy, M. M. Abo-Aly, F. A. El-Gohary
EGYPTIAN JOURNAL OF CHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Gazaa H. Morsy, Ahmad Z. Al-Herrawy, Waled M. Elsenousy, Mohamed A. Marouf
RESEARCH JOURNAL OF PHARMACEUTICAL BIOLOGICAL AND CHEMICAL SCIENCES
(2016)