Article
Immunology
Gesine Behrens, Stephanie L. Edelmann, Timsse Raj, Nina Kronbeck, Thomas Monecke, Elena Davydova, Elaine H. Wong, Lisa Kifinger, Florian Giesert, Martin E. Kirmaier, Christine Hohn, Laura S. de Jonge, Mariano Gonzalez Pisfil, Mingui Fu, Sebastian Theurich, Stefan Feske, Naoto Kawakami, Wolfgang Wurst, Dierk Niessing, Vigo Heissmeyer
Summary: The proteins Roquin-1 and Regnase-1 play crucial roles in maintaining immune homeostasis, with mutations leading to systemic lupus erythematosus-like phenotypes and autoimmunity. Additionally, these mutations affect T cell activation, metabolic reprogramming, and autoimmunity processes.
Article
Environmental Sciences
Liheng Liu, Ronghao Yu, Shixiong Zhao, Xingfeng Cao, Xuehong Zhang, Shaoyuan Bai
Summary: The treatment performance of a heterogeneous Fenton system driven by iron-loaded sludge biochar (Fe-BC) on wastewater containing sulfamethoxazole (SMX) was investigated in this study. The batch experiments determined the optimal operating conditions and achieved a high CODcr removal efficiency. The removal of CODcr was described by the BMG and BMGL models, and a diffusion-controlled process was observed. The contributions of adsorption and Fenton oxidation to CODcr removal were determined to be 42.79%, 54.01%, and 3.20%, respectively. Homogeneous Fenton degradation pathways for SMX were also identified.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Oncology
Sharmila Raghunandan, Melinda Pauly, William G. Blum, Muna Qayed, Madhav Dhodapkar, Mohamed Elkhalifa, Benjamin Watkins, Michelle Schoettler, Edwin Horwitz, Suhag Parikh, Shanmuganathan Chandrakasan, Kathryn Leung, Elyse Bryson, Laura Deeb, Jonathan L. Kaufman, Diana Worthington-White, Adina Alazraki, Jordan M. Schecter, Deepu Madduri, Carolyn C. Jackson, Enrique Zudaire, Agne Taraseviciute-Morris, Alexander Babich, Tonia Nesheiwat, Martin Vogel, Nikoletta Lendvai, Lida Pacaud, Kirsten M. Williams
Summary: Plasmablastic lymphoma (PBL) is a rare subtype of aggressive large B-cell lymphoma that has a poor prognosis. Treatment options are limited and new approaches are needed. This case report highlights the successful use of chimeric antigen receptor T-cell (CAR-T) therapy targeting B-cell maturation antigen (BCMA) in a patient with refractory PBL, resulting in a complete remission without severe adverse effects. This supports the consideration of immunotherapy as a potential treatment option for refractory PBL.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Chemistry, Multidisciplinary
Wenyue Cao, Zhi Zachary Geng, Na Wang, Quan Pan, Shaodong Guo, Shiqing Xu, Jianfeng Zhou, Wenshe Ray Liu
Summary: In this study, a novel CAR design was developed to achieve controllable activation of CAR-T cells by displaying anti-CD19 scFv in the presence of the HCV-NS3 inhibitor ASV. The intact CAR on T cells can be turned on and off in a dose dependent manner by controlling the presence of ASV, enabling delicate modulation of CAR-T activation during cancer treatment.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Oncology
Esther Schoutrop, Thomas Poiret, Ibrahim El-Serafi, Ying Zhao, Rui He, Alina Moter, Johan Henriksson, Moustapha Hassan, Isabelle Magalhaes, Jonas Mattsson
Summary: This study demonstrates the enhanced therapeutic potential of MSLN-CAR T cells expressing a mutated CD3 zeta chain containing a single ITAM for the treatment of ovarian cancer. CAR T cells with calibrated activation potential may improve clinical responses in solid tumors.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Multidisciplinary Sciences
Markus Blatter, Charlotte Meylan, Antoine Clery, Roberto Giambruno, Yaroslav Nikolaev, Michel Heidecker, Jessica Arvindbhai Solanki, Manuel O. Diaz, Davide Gabellini, Frederic H. -T. Allain
Summary: The study found that Mixed-lineage leukemia 1 (MLL1) is a transcription activator of the HOX family and its activity is repressed by cyclophilin 33 (Cyp33), which binds to MLL1 PHD3. The researchers determined the solution structures of Cyp33 in different binding states and identified that conformational changes triggered by Cyp33 binding to RNA facilitate the release of MLL1 from the histone mark. These findings provide a mechanistic understanding of how Cyp33 binding to MLL1 switches chromatin to a transcriptional repressive state through a negative feedback loop triggered by RNA binding.
Article
Immunology
Thessa Laeremans, Sabine den Roover, Cynthia Lungu, Sigrid D'haese, Rob A. A. Gruters, Sabine D. D. Allard, Joeri L. L. Aerts
Summary: This study evaluated the effects of a therapeutic DC-based vaccine consisting of monocyte-derived DCs electroporated with HIV-1 Tat, Rev and Nef encoding mRNA on NK cell frequency, phenotype and functionality in HIV-1-infected individuals. The results showed a significant increase in cytotoxic NK cells, changes in NK cell phenotype associated with migration and exhaustion, as well as improved NK cell-mediated killing and (poly)functionality following immunisation. These findings highlight the importance of evaluating NK cells in future clinical trials of DC-based immunotherapy in the context of HIV-1 infection.
Article
Chemistry, Medicinal
Serat-E Ali, Xiaoli Meng, Laila Kafu, Sean Hammond, Qing Zhao, Monday Ogese, Rowena Sison-Young, Robert Jones, Benjamin Chan, Lucia Livoti, Yonghu Sun, Lele Sun, Hong Liu, Anthony Topping, Christopher Goldring, Furen Zhang, Dean John Naisbitt
Summary: This study utilized metabolite-responsive T-cells and primary human hepatocytes to establish a hepatocyte immune cell coculture system for detecting metabolite formation and metabolite-specific T-cell responses. The results showed that metabolites could be transferred between hepatocytes and immune cells and stimulate T-cell proliferation. This study demonstrates the use of hepatocyte immune cell coculture systems for detecting in situ metabolite formation and metabolite-specific T-cell responses.
CHEMICAL RESEARCH IN TOXICOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Dylan M. T. Eralie, Tessa M. Hoang, Justin A. Williamson, Daniel K. Unruh, John D. Gorden, Anne E. V. Gorden
Summary: In this work, five cerium-(IV) complexes were synthesized, three of which were structural isomorphs from the same pyrasal ligand. The solid-state result was identified through structural analysis, which depended on the initial pH of the reaction solution and the temperature. The pyrasal ligands used in this study have weaker binding due to the electron-withdrawing effect of the nitrogen atoms within the pyrazine ring. The electron-withdrawing effect also deactivates the second amine after the first condensation with salicylaldehyde. Even with extended reaction times and excess ligand, without a metal template, only one salicylaldehyde is added.
INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Zhengquan Yang, Chengliang Zhang, Guojun Lian, Shijie Dong, Menghui Song, Hengrong Shao, Jingmei Wang, Tao Zhong, Zhenni Luo, Shengnan Jin, Chunming Ding
Summary: This study presents a newly discovered Pfu RNA ligase in the hyperthermophilic archaea Pyrococcus furiosus and an engineered fusion enzyme that can catalyze the production of adenylated products from DNA substrates. These enzymes may serve as useful tools for applications involving AppOligos.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Christopher Szeto, Pirooz Zareie, Rushika C. Wirasinha, Justin B. Zhang, Andrea T. Nguyen, Alan Riboldi-Tunnicliffe, Nicole L. La Gruta, Stephanie Gras, Stephen R. Daley
Summary: This study describes the contribution of natural covalent bonds between TCR and antigenic peptides in T cell differentiation and activation. The formation of disulfide bonds between TCR and peptide does not require structural rearrangement and can still occur when the initial affinity is low, facilitating T cell activation.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Landon J. Edgar, Andrew J. Thompson, Vincent F. Vartabedian, Chika Kikuchi, Jordan L. Woehl, John R. Teijaro, James C. Paulson
Summary: Sialic acid-containing glycans on the surface of T cells and APCs are alternative ligands of CD28 that compete with CD80 for binding, resulting in attenuated costimulation. Removal of sialic acids enhances antigen-mediated activation of naive T cells and increases revival of effector T cells through synergistic mechanism with PD-1 axis antibody blockade. These findings reveal a previously unrecognized role of sialic acid ligands in attenuation of CD28-mediated costimulation of T cells.
ACS CENTRAL SCIENCE
(2021)
Article
Engineering, Chemical
Jiong Wang, Zhibin Liu, Zhirong Sun
Summary: A graphite felt electrode material supporting a Cu-based catalyst was prepared by a hydrothermal process and subsequent heat treatment, and a heterogeneous electro-Fenton system was built to investigate the degradation of sulfamethoxazole. The composite cathode CuHDC-400/GF achieved 100% removal of SMX within 45 min and showed efficient degradation over a wide pH range. The in-situ catalysis of H2O2 by CuHDC-400/GF reduced catalyst consumption and enhanced electrode stability, and the active superoxide radical O-center dot(2)- was identified as the major contributor to SMX degradation.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Marie-Anais Locquet, Gabriel Ichim, Joseph Bisaccial, Aurelie Dutour, Serge Lebeque, Marie Castets, Kathrin Weber
Summary: In cancer cells, activation of TLR3 without Caspase-8 can trigger a new form of cell death that is distinct from classical apoptosis. This type of cell death is associated with lysosomal permeabilization, which occurs upstream of caspase activation and represents a default death mechanism in the absence of Caspase-8.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Francesco Bartoli, Luca Conti, Giammarco Maria Romano, Lara Massai, Paola Paoli, Patrizia Rossi, Giangaetano Pietraperzia, Cristina Gellini, Andrea Bencini
Summary: The synthesis of three new polyamine receptors based on a common cyclen platform with different appended fluorophores was reported. The study showed that the structural features of these receptors are controlled by a balance between hydrogen bonding and electrostatic repulsions in their protonated species, while their emission properties are tuned by photoinduced electron and/or proton transfer processes.
NEW JOURNAL OF CHEMISTRY
(2021)
Review
Allergy
Werner J. Pichler, Jacqueline Adam, Stephen Watkins, Natascha Wuillemin, James Yun, Daniel Yerly
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2015)
Article
Allergy
Lisa M. Wheatley, Marshall Plaut, Julie M. Schwaninger, Aleena Banerji, Mariana Castells, Fred D. Finkelman, Gerald J. Gleich, Emma Guttman-Yassky, Simon A. K. Mallal, Dean J. Naisbitt, David A. Ostrov, Elizabeth J. Phillips, Werner J. Pichler, Thomas A. E. Platts-Mills, Jean-Claude Roujeau, Lawrence B. Schwartz, Lauren A. Trepanier
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2015)
Article
Allergy
Yuttana Srinoulprasert, Werner J. Pichler
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2014)
Article
Immunology
James Yun, Maria J. Marcaida, Klara K. Eriksson, Heidi Jamin, Stefano Fontana, Werner J. Pichler, Daniel Yerly
JOURNAL OF IMMUNOLOGY
(2014)
Article
Multidisciplinary Sciences
Jacqueline Adam, Natascha Wuillemin, Stephan Watkins, Heidi Jamin, Klara K. Eriksson, Peter Villiger, Stefano Fontana, Werner J. Pichler, Daniel Yerly
Review
Allergy
Werner J. Pichler
Article
Allergy
Lukas Jorg, Daniel Yerly, Arthur Helbling, Werner Pichler
Review
Allergy
Werner J. Pichler
Summary: The understanding of IgE-mediated drug allergies has been challenged by the hapten concept, proposing that mast cell reactivity to drugs may be reduced, some drug-protein complexes can trigger allergic reactions, and the slower formation of covalent hapten-protein adducts allows time for mast cell desensitization.
Review
Allergy
Werner J. Pichler
Summary: Drug hypersensitivity reactions are characterized by abnormal immune stimulation driven by non-covalent drug-protein interactions, including mimicry, increased antibody affinity, and p-i stimulation. These interactions can lead to various complications such as viral reactivations, autoimmunity, and multiple drug hypersensitivity.
Article
Allergy
Werner J. Pichler, Marie-Charlotte Brueggen
Summary: Virus infections can enhance drug hypersensitivity reactions by inducing immune stimulation and increasing the interaction with immune receptors. In some cases, severe drug hypersensitivity reactions may precede the reactivation of herpes viruses. This phenomenon can be explained by the immune stimulation during drug reactions, which activates herpes-specific T cells and leads to the release of herpes viruses. These concepts explain the transient nature of drug hypersensitivity reactions during viral infections and the asymptomatic herpes-virus viremia after severe drug hypersensitivity reactions.
Review
Biochemistry & Molecular Biology
Stephan L. Watkins
Summary: There has been a significant increase in the use of molecular dynamics simulations in research and industry, particularly in the study of neurological and biological membranes. This review examines both recent and older studies to assess the evolution of the field. The analysis provides an overview of current methodologies, trends, and predictions for the future of membrane molecular dynamics.
Biographical-Item
Allergy
Arthur Helbling, Oliver Hausmann, Marek Jutel, Cezmi A. Akdis, Werner J. Pichler
Review
Allergy
Werner J. Pichler, Lester Thoo, Daniel Yerly
Summary: Eosinophilia is a common manifestation in drug hypersensitivity reactions, and its cause is unclear. However, it seems to be related to off-target activities of drugs with immune receptors, resulting in T-cell stimulation and excessive IL-5 production. This IL-5-TCR signalosome hypothesis may explain eosinophilia in various circumstances and provide new therapeutic options for eosinophilic diseases.
Review
Allergy
Werner J. Pichler, Stephen Watkins, Daniel Yerly
Summary: Drug hypersensitivity reactions are unique because they are not driven by antigens, but by non-covalent drug-protein binding. These new models could impact risk assessments in drug development, but early detection of such interactions is challenging. A combination of in vitro cell culture assays, functional analyses, and structural analysis may be a realistic approach to detect clinically relevant non-covalent drug interactions.
FRONTIERS IN ALLERGY
(2022)
Article
Dermatology
B. Wuethrich, P. C. Frei, A. Bircher, C. Hauser, W. Pichler, P. Schmid-Grendelmeler, F. Spertini, D. Olgiati, U. Mueller
AKTUELLE DERMATOLOGIE
(2014)