期刊
PLOS ONE
卷 8, 期 11, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0077955
关键词
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资金
- Chinese Academy of Sciences
- Natural Science Foundation of China [91029707, 31170868]
- Shanghai Natural Science foundation [11ZR1442600]
- Novo Nordisk-CAS Research Foundation
- SA-SIBS Scholarship Program
- National Key Programs on Infectious Disease [2012ZX10002007-003]
Background: Mycoplasma hyorhinis (M.hyorhinis, M.hy) is associated with development of gastric and prostate cancers. The NLRP3 inflammasome, a protein complex controlling maturation of important pro-inflammatory cytokines interleukin (IL)-1 beta and IL-18, is also involved in tumorigenesis and metastasis of various cancers. Methodology/Principal Findings: To clarify whether M.hy promoted tumor development via inflammasome activation, we analyzed monocytes for IL-1 beta and IL-18 production upon M.hy challenge. When exposed to M.hy, human monocytes exhibited rapid and robust IL-1 beta and IL-18 secretion. We further identified that lipid-associated membrane protein (LAMP) from M.hy was responsible for IL-1 beta induction. Applying competitive inhibitors, gene specific shRNA and gene targeted mice, we verified that M.hy induced IL-1 beta secretion was NLRP3-dependent in vitro and in vivo. Cathepsin B activity, K+ efflux, Ca2+ influx and ROS production were all required for the NLRP3 inflammasome activation by M.hy. Importantly, it is IL-1 beta but not IL-18 produced from macrophages challenged with M.hy promoted gastric cancer cell migration and invasion. Conclusions: Our data suggest that activation of the NLRP3 inflammasome by M.hy may be associated with its promotion of gastric cancer metastasis, and anti-M.hy therapy or limiting NLRP3 signaling could be effective approach for control of gastric cancer progress.
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