Article
Virology
Stephane Isnard, Lena Royston, John Lin, Brandon Fombuena, Simeng Bu, Sanket Kant, Tsoarello Mabanga, Carolina Berini, Mohamed El-Far, Madeleine Durand, Cecile L. Tremblay, Nicole F. Bernard, Guido Kroemer, Jean-Pierre Routy
Summary: HIV elite controllers (ECs) have lower levels of extracellular acyl coenzyme A binding protein (ACBP) compared to people living with HIV (PLWH) receiving antiretroviral therapy (ART) or not. ACBP levels in ECs are not influenced by protective HLA alleles or immune responses, but may be associated with interleukin-1 beta levels in ART-treated PLWH. The ACBP pathway could be a potential target in HIV cure strategies.
Article
Microbiology
Ziyu Ma, Muhammad Akhtar, Hong Pan, Qiyao Liu, Yan Chen, Xinxin Zhou, Yingting You, Deshi Shi, Huazhen Liu
Summary: Intestinal inflammation is a concerning issue in chicken production worldwide and is associated with Th17/Treg cell imbalance. This study found that reshaping gut microbiota through fecal microbiota transplantation (FMT) improved chicken growth by balancing Th17/Treg cells. Transferring fecal microbiota from healthy donors with better growth performance and abundant Lactobacillus to young chicks significantly increased growth performance, decreased Th17 cell-associated factors and cytokines, and increased Treg cell-associated factors and cytokines in the jejunum.
Article
Multidisciplinary Sciences
Laura Capa, Ruben Ayala-Suarez, Humberto Erick De La Torre Tarazona, Juan Gonzalez-Garcia, Jorge del Romero, Jose Alcami, Francisco Diez-Fuertes
Summary: This study retrospectively collected clinical and epidemiological data from 313 LTNP individuals in 21 Spanish hospitals and followed LTNP individuals who maintained CD4+ T cell counts over 500 cells/mu l and viral loads under 10,000 copies/mL for at least 10 years. EC-LTNP phenotype was associated with improved survival and slower disease progression compared with other phenotypes of LTNP, making them strong candidates for a model of functional cure of HIV-1 infection.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Jeromine Klingler, Nicodeme Paul, Geraldine Laumond, Sylvie Schmidt, Luzia M. Mayr, Thomas Decoville, Olivier Lambotte, Brigitte Autran, Seiamak Bahram, Christiane Moog
Summary: The study found that HIV-1 controllers have a unique HIV-specific antibody profile that may contribute to the control of HIV replication. This emphasizes the importance of analyzing multifunctional antibody responses in vaccine research.
Review
Virology
Joseph Hokello, Priya Tyagi, Shelly Dimri, Adhikarimayum Lakhikumar Sharma, Mudit Tyagi
Summary: HIV-exposed seronegative individuals (HESIs) are individuals who are exposed to HIV multiple times but do not show signs of infection. Long-term non-progressors (LTNPs) are HIV-infected individuals who remain stable without therapy, while elite controllers have very low viral levels without treatment. The mechanisms behind these groups' ability to control HIV are multifaceted, involving genetic, immunological, and viral factors.
Review
Medicine, Research & Experimental
Jonathan Z. Li, Joel N. Blankson
Summary: A small percentage of HIV-1 patients, known as elite controllers (ECs) or posttreatment controllers (PTCs), can control viral replication without antiretroviral therapy. Although these patients may serve as a model for a functional cure for HIV-1, the mechanisms responsible for viral control remain unclear. Research on controllers provides important insights for HIV cure research.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Cell Biology
Shengwei Jin, Siyuan Sun, Hanzhi Ling, Jinglan Ma, Xu Zhang, Zhen Xie, Ning Zhan, Wenjie Zheng, Man Li, Yang Qin, Heping Zhao, Yan Chen, Xinyu Yang, Jianguang Wang
Summary: Protectin DX (PDX) was found to effectively ameliorate the progression of collagen-induced arthritis by restoring Treg/T(h)17 cell balance, and this effect was mediated by inhibiting the NLRP3 inflammasome pathway via miR-20a.
CELL DEATH & DISEASE
(2021)
Article
Virology
Cynthia Lungu, Ronald J. Overmars, Esmee Grundeken, Patrick H. M. Boers, Marchina E. van der Ende, Thibault Mesplede, Rob A. Gruters
Summary: Although some individuals with HIV-2 develop AIDS, most may not. The replication rates of isolated viruses differ between non-progressors and progressors. This study did not find genotypic defects that contribute to HIV-2 non-progression, indicating that progression may be determined before or during integration of the virus.
Article
Multidisciplinary Sciences
Behzad Etemad, Xiaoming Sun, Yijia Li, Meghan Melberg, Daniela Moisi, Rachel Gottlieb, Hayat Ahmed, Evgenia Aga, Ronald J. Bosch, Edward P. Acosta, Yuko Yuki, Maureen P. Martin, Mary Carrington, Rajesh T. Gandhi, Jeffrey M. Jacobson, Paul Volberding, Elizabeth Connick, Ronald Mitsuyasu, Ian Frank, Michael Saag, Joseph J. Eron, Daniel Skiest, David M. Margolis, Diane Havlir, Robert T. Schooley, Michael M. Lederman, Xu G. Yu, Jonathan Z. Li
Summary: This study evaluated the mechanisms of HIV post-treatment control and found that post-treatment controllers (PTCs) had stable HIV reservoirs and better immunological profiles. These findings have implications for future studies aiming at achieving an HIV functional cure.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Pharmacology & Pharmacy
Xiaoyan Shi, Hanwen Huang, Min Zhou, Yarong Liu, Hongfei Wu, Min Dai
Summary: The study demonstrated that Pae can restrict AS development and reduce collagen deposition. The antifibrosis effect of Pae is achieved by regulating gut microbiota, enhancing the Treg/Th17 balance and suppressing the expression of fibrosis-related indicators.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Immunology
Long Wen, Lei Shi, Xiang-Long Kong, Ke-Yu Li, Hui Li, Di-Xuan Jiang, Fan Zhang, Zhi-Guo Zhou
Summary: This study explores the potential mechanism of fecal microbiota transplantation (FMT) in the treatment of Pseudomonas aeruginosa pneumonia. The results show that FMT can restore the balance of Treg/Th17 cells, improve inflammation and lung injury by regulating gut microbiota disturbance and metabolic disorder.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Medicine, Research & Experimental
Yaoyao Chang, Lixiang Zhai, Jiao Peng, Haiqiang Wu, Zhaoxiang Bian, Haitao Xiao
Summary: IBD is a chronic inflammatory intestinal disorder with limited treatment options, and correcting the imbalance of Th17/Treg has been shown to be effective in preventing and treating IBD. Phytochemicals derived from natural products have been recognized as potent regulators of Th17/Treg and may offer protective benefits against colonic inflammation, showing great potential for the development of new drugs for IBD treatment.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Immunology
Yuting Shi, Jinming Su, Rongfeng Chen, Wudi Wei, Zongxiang Yuan, Xiu Chen, Xinwei Wang, Hao Liang, Li Ye, Junjun Jiang
Summary: The natural progression of HIV-1 infection leads to acquired immunodeficiency syndrome (AIDS), but long-term nonprogressors (LTNPs) and elite controllers (ECs) who can naturally control the virus offer hope for a functional cure. Innate immunity activation plays a key role in HIV-1 infection, and understanding its regulation and disease progression mechanism can contribute to the cure of AIDS.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Shuting Wen, Long He, Zhuotai Zhong, Runyuan Zhao, Senhui Weng, Hong Mi, Fengbin Liu
Summary: Stigmasterol treatment restores the Treg/Th17 balance and alters the gut microbiota, while also enhancing the production of SCFAs, particularly butyrate. Mechanistically, butyrate activates PPAR gamma and reprograms energy metabolism to promote Treg differentiation and inhibit Th17 differentiation, thus attenuating IBD.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Infectious Diseases
Rafael Cena-Diez, Aswathy Narayanan, Shilpa Ray, Maarten van de Klundert, Jimmy E. Rodriguez, Johan Nilvebrant, Per-Ake Nygren, Akos Vegvari, Robert van Domselaar, Anders Sonnerborg
Summary: WC-am, a dipeptide, has been found to be increased in elite controllers, who can control HIV-1 infection. This study aimed to evaluate the anti-HIV-1 activity and mechanism of action of WC-am. The results showed that WC-am binds to the CD4 binding pocket of HIV-1 gp120, blocking its binding to host cell receptors, and inhibits HIV-1 replication by affecting reverse transcription.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Physiology
Mieke Veltman, Marta Stolarczyk, Danuta Radzioch, Gabriella Wojewodka, Juan B. De Sanctis, Willem A. Dik, Oleh Dzyubachyk, Tamas Oravecz, Isme de Kleer, Bob J. Scholte
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2016)
Review
Pharmacology & Pharmacy
Cynthia Kanagaratham, Danuta Radzioch
CURRENT PHARMACEUTICAL DESIGN
(2016)
Article
Nanoscience & Nanotechnology
Ouajdi Felfoul, Mahmood Mohammadi, Samira Taherkhani, Dominic de Lanauze, Yong Zhong Xu, Dumitru Loghin, Sherief Essa, Sylwia Jancik, Daniel Houle, Michel Lafleur, Louis Gaboury, Maryam Tabrizian, Neila Kaou, Michael Atkin, Te Vuong, Gerald Batist, Nicole Beauchemin, Danuta Radzioch, Sylvain Martel
NATURE NANOTECHNOLOGY
(2016)
Article
Genetics & Heredity
Dusan Garic, Juan B. De Sanctis, Gabriella Wojewodka, Daniel Houle, Shanon Cupri, Asmahan Abu-Arish, John W. Hanrahan, Marian Hajduch, Elias Matouk, Danuta Radzioch
JOURNAL OF MOLECULAR MEDICINE-JMM
(2017)
Article
Genetics & Heredity
Cynthia Kanagaratham, Pierre Camateros, John Ren, Robert Sladek, Silvia M. Vidal, Danuta Radzioch
G3-GENES GENOMES GENETICS
(2017)
Article
Infectious Diseases
J-P Routy, J. B. Angel, M. Patel, C. Kanagaratham, D. Radzioch, I. Kema, N. Gilmore, P. Ancuta, J. Singer, M-A Jenabian
Article
Multidisciplinary Sciences
Nasheed Moqueet, Cynthia Kanagaratham, M. John Gill, Mark Hull, Sharon Walmsley, Danuta Radzioch, Sahar Saeed, Robert W. Platt, Marina B. Klein
Article
Respiratory System
Dugan Garic, Shao Tao, Eisha Ahmed, Mina Youssef, Cynthia Kanagaratham, Juhi Shah, Bruce Mazer, Danuta Radzioch
JOURNAL OF CYSTIC FIBROSIS
(2019)
Article
Chemistry, Multidisciplinary
Mina Youssef, Juan B. De Sanctis, Cynthia Kanagaratham, Shao Tao, Eisha Ahmed, Danuta Radzioch
PHARMACEUTICAL RESEARCH
(2020)
Review
Biochemistry & Molecular Biology
Dusan Garic, Daciana Catalina Dumut, Juhi Shah, Juan Bautista De Sanctis, Danuta Radzioch
CELLULAR AND MOLECULAR LIFE SCIENCES
(2020)
Article
Respiratory System
Mina Youssef, Juan B. De Sanctis, Juhi Shah, Daciana Catalina Dumut, Marian Hajduch, Basil J. Petrof, Danuta Radzioch
Article
Nutrition & Dietetics
Juan Bautista De Sanctis, Daciana Catalina Dumut, Danuta Radzioch, Marian Hajduch
Article
Microbiology
Lisa C. Hennemann, Shantelle L. LaFayette, Julien K. Malet, Perrine Bortolotti, Tianxiao Yang, Geoffrey A. McKay, Daniel Houle, Danuta Radzioch, Simon Rousseau, Dao Nguyen
Summary: Loss-of-function lasR mutants of Pseudomonas aeruginosa induce increased levels of airway epithelial ICAM-1, leading to enhanced neutrophilic inflammation and exacerbating lung disease progression. This study sheds light on the intricate interplay between lasR mutants, LasR-regulated proteases, and airway epithelial ICAM-1 expression, revealing a new mechanism involved in the exaggerated inflammatory response induced by lasR mutants.
Article
Biochemical Research Methods
Daciana Catalina Dumut, Dusan Garic, Amanda Centorame, Danuta Radzioch
Summary: Tris-Glycine-SDS is commonly used as the running buffer for SDS-PAGE, but it has limitations for high throughput downstream applications. In this study, a novel running buffer composed of Tris, Tricine, and HEPES is proposed for simultaneous gradient-like separation of small and large proteins in a single gel.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biology
Martin Ondra, Lukas Lenart, Amanda Centorame, Daciana C. Dumut, Alexander He, Syeda Sadaf Zehra Zaidi, John W. Hanrahan, Juan Bautista De Sanctis, Danuta Radzioch, Marian Hajduch
Summary: This article describes the preparation and validation of a novel in vitro model system using CRISPR/Cas9 genome editing, which can tag the endogenously expressed WT-CFTR and detect CFTR in the plasma membrane of live cells. By inserting the HiBiT tag in WT-CFTR, a reporter luciferase with bright luminescence can be generated. Nine homozygous clones with the HiBiT knock-in were identified from the screened clones, and two clones were genetically and functionally validated. This work lays the foundation for developing unique cellular CF models by CRISPR-mediated insertion of CF-causing mutations.
LIFE SCIENCE ALLIANCE
(2023)