Article
Chemistry, Analytical
Jie Zheng, Ge-Ge Gong, Shu-Jian Zheng, Yu Zhang, Yu-Qi Feng
Summary: Carboxylic metabolites, an important class of metabolites in mammals, were successfully labeled using N-methylphenylethylamine (MPEA) to enhance detection sensitivity and chromatographic retention. The optimized method achieved high coverage analysis of carboxylated metabolites in HepG2 cells, identifying a total of 403 potential metabolites.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Brittany Widner, Melissa C. Kido Soule, Frank Xavier Ferrer-Gonzalez, Mary Ann Moran, Elizabeth B. Kujawinski
Summary: Utilizing pre-extraction derivatization and ultrahigh performance liquid chromatography electrospray ionization tandem mass spectrometry, targeted dissolved metabolites in seawater and saline culture media were successfully detected and quantified. This quantitative method has been optimized for 73 common metabolites, with 50 requiring derivatization for quantification due to low extraction efficiencies.
ANALYTICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Miaomiao Xu, Zhanpeng Zhang, Zhiyong Li, Shunyan Kan, Zhaoxiang Liu, Desheng Wang, Qihui Liu, Hua Zhang
Summary: This study analyzed NEOs and their metabolites in paired urine and blood samples collected from young adults in China. The results showed widespread detection of NEOs and their metabolites in both urine and blood samples, with gender-related differences in concentrations. Urinary levels were found to be good predictors of human exposure to NEOs and their metabolites.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Sonja A. Wrobel, Daniel Bury, Vladimir N. Belov, Jan M. Klenk, Bernhard Hauer, Heiko Hayen, Anderson J. Martino-Andrade, Holger M. Koch, Thomas Bruening, Heiko U. Kaefferlein
Summary: We have developed a specific and robust analytical method to analyze the most important neonicotinoids and their metabolites in human urine. The method was successfully validated and applied to quantify these compounds in individuals from the general population in Germany and pregnant women from Brazil with no known occupational exposures to neonicotinoids.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Md Motiur Rahaman, Rebecca S. Zwart, Thusitha W. T. Rupasinghe, Helen L. Hayden, John P. Thompson
Summary: Metabolic profiling of resistant and susceptible wheat genotypes revealed that certain metabolites constitutively expressed in resistant roots could reduce nematode reproduction, while susceptible wheat may induce metabolites that attract nematodes. The study suggests that specific metabolites may affect the strength of root cell walls and nematode penetration, highlighting differences between resistant and susceptible wheat in their response to nematode infection.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Food Science & Technology
Huihui Shen, Xiuxian Song, Yue Zhang, Peipei Zhang, Jing Li, Weijia Song, Zhiming Yu
Summary: This study investigated the BTX metabolites of a Chinese strain of Karenia brevis 165, revealing significant differences in metabolite types and concentrations at different growth stages, which can help assess ecological disaster risks.
Article
Biology
Alfredo Fabrizio Lo Faro, Anastasio Tini, Giulia Bambagiotti, Filippo Pirani, Andrea Faragalli, Flavia Carle, Elena Pacella, Artan Ceka, Marco Moretti, Massimo Gottardi, Nicola Vito Lassandro, Michele Nicolai, Marco Lupidi, Cesare Mariotti, Francesco Paolo Busardo, Jeremy Carlier
Summary: The detection of carbonic anhydrase inhibitors and their metabolites in biological samples is crucial for documenting doping misuse. This study quantified the concentrations of dorzolamide, brinzolamide, acetazolamide, and their metabolites in urine and hair samples. The results showed the necessity of setting cutoff concentrations for urinary dorzolamide and brinzolamide to avoid false positive results. Additionally, this study reported the first concentrations of brinzolamide in hair.
Article
Biochemical Research Methods
Iria Gonzalez-Marino, Ana Maria Casas-Ferreira, Miguel del Nogal Sanchez, Jose Luis Perez Pavon
Summary: A simple and fast method combining the use of a guard column-MS/MS was proposed for the determination of plasticizer metabolites in urine. The method showed high precision and low limits of quantification. It can analyze a large number of samples in a short time and achieves good agreement with UHPLC-MS/MS method.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Teresa S. McMillen, Andrew Leslie, Kenneth Chisholm, Susanne Penny, Jeffrey Gallant, Alejandro Cohen, Arik Drucker, James P. Fawcett, Devanand M. Pinto
Summary: This study presents a comprehensive and quantitatively robust method for metabolomic analysis, using HILIC and RP stationary phases combined with positive and negative electrospray ionization. The method allows for complete analysis of a single sample in as little as 40 minutes and has been validated with clinical samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Medicinal
So Hyeon Chung, Dallah Yoo, Tae-Beom Ahn, Wonwoong Lee, Jongki Hong
Summary: This study highlights the importance of profiling tryptophan metabolites in urine samples for understanding the pathological mechanisms, finding biomarkers, and discovering therapeutic targets for Parkinson's disease (PD). The developed LC-MS/MS method successfully quantified 21 tryptophan metabolites in urine samples and identified indole-3-acetic acid as a potential biomarker for PD.
Article
Chemistry, Analytical
Kerri M. Smith, Ian D. Wilson, Paul D. Rainville
Summary: The described method uses mixed-mode reversed-phase chromatography on a CSH Phenyl-Hexyl column for the analysis of organic acids, showing good resolution and retention time reproducibility. When applied to comparative metabolomic analysis of urine samples from healthy controls and breast cancer positive subjects, the method demonstrated excellent performance in distinguishing between the two groups.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Saleh Alseekh, Asaph Aharoni, Yariv Brotman, Kevin Contrepois, John D'Auria, Jan Ewald, Jennifer C. Ewald, Paul D. Fraser, Patrick Giavalisco, Robert D. Hall, Matthias Heinemann, Hannes Link, Jie Luo, Steffen Neumann, Jens Nielsen, Leonardo Perez de Souza, Kazuki Saito, Uwe Sauer, Frank C. Schroeder, Stefan Schuster, Gary Siuzdak, Aleksandra Skirycz, Lloyd W. Sumner, Michael P. Snyder, Huiru Tang, Takayuki Tohge, Yulan Wang, Weiwei Wen, Si Wu, Guowang Xu, Nicola Zamboni, Alisdair R. Fernie
Summary: Compound identification and reliable quantification in metabolomics are complicated by the chemical complexity and dynamic range of the metabolome, while quantification in complex mixtures can be further complicated by ion suppression and the presence of isomers.
Article
Biochemical Research Methods
Elizabeth Holton, Barbara Kasprzyk-Hordern
Summary: The manuscript describes a new multiresidue method using UPLC-MS/MS and MRM for the identification and quantification of antibiotics and their metabolites in various environmental matrices. The method shows good instrumental performance with high accuracy and precision. Method quantification limits are low in river water, wastewater, river sediment, and suspended solids, and it has been successfully validated for the quantification of analytes in both aqueous and solid samples. Some analytes are considered on a semi-quantitative basis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Parasitology
Mingxing Zhu, Xiancai Du, Hongxia Xu, Songhao Yang, Chan Wang, Yazhou Zhu, Tingrui Zhang, Wei Zhao
Summary: Metabolomics approach revealed that the metabolism of nucleotides, alkaloids, amino acids, amides, and organic acids in mice is closely interrelated with E. granulosus infection. The study found that metabolic pathways in the liver such as tyrosine and tryptophan biosynthesis, phenylalanine, valine, leucine and isoleucine biosynthesis, and phenylalanine metabolism were notably associated with the occurrence and development of hydatid disease.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Minnie Jacob, Refat M. Nimer, Mohamad S. Alabdaljabar, Essa M. Sabi, Mysoon M. Al-Ansari, Maged Housien, Khalid M. Sumaily, Lina A. Dahabiyeh, Anas M. Abdel Rahman
Summary: This study used liquid chromatography-mass spectrometry (LC-MS) metabolomics to analyze the metabolome of serum from patients with nephrotic syndrome (NS) and found significant changes in 176 metabolites compared to the control group. Dysregulation of arginine, proline, and tryptophan metabolism, as well as arginine, phenylalanine, tyrosine, and tryptophan biosynthesis, were the most common metabolic pathways affected in NS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)