Article
Food Science & Technology
Juliana A. S. Leite, Randall C. Robinson, Jaime Salcedo, Juliana N. R. Ract, Virginia S. Quintal, Carmen C. Tadini, Daniela Barile
Summary: This study investigated the impact of microwave-assisted heating on the concentration of key biological compounds in donor human milk. Compared to low-temperature long-time pasteurization, microwave-assisted heating better preserved the concentration of immunoglobulins and lactoferrin, making it a promising alternative method for Human Milk Banks.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Chemistry, Applied
Eva Kontopodi, Kasper Hettinga, Bernd Stahl, Johannes B. van Goudoever, Ruurd M. van Elburg
Summary: Holder pasteurization is the current recommended method for donor human milk treatment, effectively eliminating most life-threatening contaminants but reducing biological properties. Developments in novel processing methods to retain functional components of donor milk are of growing interest. A multifactorial approach and complementary assays are essential for assessing the suitability of new methods.
Article
Food Science & Technology
Yong Hu, Eva Kontopodi, Elise Mank, Chris H. P. van den Akker, Johannes B. van Goudoever, Kasper Hettinga, Ruurd M. van Elburg, Johannes Thaler, Rienk Nieuwland
Summary: Donor human milk is the first alternative for preterm infants when mother's own milk is not available. Most available human milk banking guidelines recommend classical holder pasteurization to ensure safety by eliminating potential infectious microorganisms. Processing by heat treatment, however, negatively affects functionality and availability of bioactive components naturally present in human milk. Here we compared the effect of five different processing methods on the ability of human milk to induce blood plasma clotting, and found that ultraviolet-C irradiation and high-pressure processing were best at preserving this activity.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2022)
Article
Pediatrics
Eva Kontopodi, Bernd Stahl, Johannes B. van Goudoever, Sjef Boeren, Rian A. H. Timmermans, Heidy M. W. den Besten, Ruurd M. Van Elburg, Kasper Hettinga
Summary: This study investigated high-pressure processing (HPP), ultraviolet-C irradiation (UV-C), and thermoultrasonication (TUS) as alternatives to thermal pasteurization (HoP) for donor human milk treatment. The results showed that HPP and UV-C may be considered as suitable alternatives to HoP, as they achieved sufficient microbial inactivation while better preserving the bioactive components of the milk.
FRONTIERS IN PEDIATRICS
(2022)
Article
Nutrition & Dietetics
Isadora Beghetti, Monica Barone, Luigia De Fazio, Eleonora Laderchi, Elena Biagi, Silvia Turroni, Patrizia Brigidi, Andrea Pession, Luigi Corvaglia, Arianna Aceti
Summary: Human milk is the best feeding option for preterm infants. When mother's milk is not available, pasteurized donor human milk is the best alternative. This study compared the microbiota of pasteurized donor human milk with the milk from mothers who delivered prematurely and assessed the effect of pasteurization on the microbiota. The results showed that pasteurized donor human milk had higher alpha diversity and a different composition compared to milk from mothers who delivered prematurely. Pasteurization also had an impact on the microbiota structure of human milk.
Article
Food Science & Technology
Zhibin Li, Dasong Liu, Shu Xu, Wenjin Zhang, Yacine Hemar, Joe M. Regenstein, Peng Zhou
Summary: This study found that HTST and UV-C technologies could significantly reduce the number of bacteria in skim milk, with MF technology being the most effective in reducing bacteria. UV-C and MF were able to completely eliminate coliforms, while HTST was unaffected. The retention rates of various bioactive proteins/enzymes differed under different treatment methods, with MF-0.8 and MF-1.4 showing the best results.
Article
Immunology
Claudio Rodriguez-Camejo, Arturo Puyol, Paula Arbildi, Cecilia Sonora, Laura Fazio, Gabriela Sire, Ana Hernandez
Summary: This study compared the effects of raw versus pasteurized milk and colostrum versus mature milk on cellular functions associated with the gut epithelial barrier and responses to inflammatory stimuli. The results showed that all types of milk stimulated epithelial cell proliferation. However, only raw colostrum increased cell migration and interfered with the interaction between E. coli and epithelial cells. Moreover, only raw colostrum enhanced the response of epithelial and macrophage cells to lipopolysaccharide (LPS), while both raw and pasteurized milk diminished the LPS induced response in monocytes.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Applied
Jie Zhang, John A. Duley, David M. Cowley, Paul N. Shaw, Pieter Koorts, Nidhi Bansal
Summary: This study compared the effects of Holder pasteurization (HoP) and hydrostatic high-pressure (HHP) processing on human milk proteins. The results showed that HHP processing better preserved the protein profile of human milk compared to HoP.
Article
Food Science & Technology
Mutamed Ayyash, Abdelmoneim Abdalla, Basim Abu-Jdayil, Thom Huppertz, Raman Bhaskaracharya, Saleha Al-Mardeai, Anusha Mairpady, Arachchige Ranasinghe, Anas Al-Nabulsi
Summary: The study found that high-pressure processing and ultra-high temperature treatment can enhance the rheological properties of fermented camel milk, with the lowest viscosity observed in fermented milk treated with low-temperature long-time and high-temperature short-time methods.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Nutrition & Dietetics
Elise Mank, Eva Kontopodi, Annemieke C. Heijboer, Ruurd M. van Elburg, Kasper Hettinga, Johannes B. van Goudoever, Letty van Toledo
Summary: This study compared the effect of different techniques on the insulin concentration in donor human milk (DHM) and found that thermoultrasonication (TUS), ultraviolet-C irradiation (UV-C), and high-pressure processing (HPP) can preserve insulin in DHM. These new techniques may serve as alternatives to holder pasteurization (HoP).
CLINICAL NUTRITION
(2021)
Article
Microbiology
Lisa F. Stinson, Donna T. Geddes, Lucy L. Furfaro
Summary: Human milk is the best nutrition source for infants and contains bioactive compounds and microorganisms. Pasteurization and UV-C irradiation are both used to prevent pathogen transmission, but UV-C may be more effective in eliminating potential phages that could affect infant gut colonization. Further studies are needed to explore the impact of pasteurization methods on bacteriophage titres in human milk.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Food Science & Technology
Dario Fernando Guaman-Lozada, Daniel Chuquin Vasco, Mabel Parada Rivera, Gisela Pilco Bonilla
Summary: This study investigated the effect of UV-C radiation on the bacterial count of raw and pasteurized milk contaminated with Escherichia coli at different thickness levels. The results showed that the bacterial count decreased exponentially with increasing UV-C dose and that the effect of the dose was more pronounced at lower thicknesses. The statistical analysis revealed that the developed model was highly significant, with an adjusted R-squared of 0.981. Additionally, the quality and composition of the milk treated with UV-C were not affected.
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
(2023)
Article
Food Science & Technology
Miroslava Jandova, Pavel Mericka, Michaela Fiserova, Ales Landfeld, Pavla Paterova, Lenka Hobzova, Eva Jarkovska, Marian Kacerovsky, Milan Houska
Summary: A systematic study conducted from 2017 to 2020 examined the presence of B. cereus in pasteurized banked human milk (PBM), finding a significant increase in positive tests during the summer months. Bacterial contamination, with B. cereus being the most common contaminant, resulted in an annual discard rate of PBM ranging from 8.7% to 10.0%. Additionally, enterotoxin production was detected in 15% of the tested samples, and most samples had B. cereus CFU-quantities below 10 CFU/mL. These quantitative data provide insights for risk assessment in the storage and handling of PBM.
Article
Chemistry, Applied
Katherine Blackshaw, Jiadai Wu, Nicholas Proschogo, Justin Davies, Daniel Oldfield, Aaron Schindeler, Richard B. Banati, Fariba Dehghani, Peter Valtchev
Summary: This study evaluated the application of freeze-drying and low-dose gamma irradiation for long-term storage of donor human milk (DHM). The results showed that this method effectively preserved the quality of DHM and could be a safe and convenient way to store and deliver it at ambient temperature, making it suitable for emergencies and humanitarian aid.
Article
Pediatrics
Irene Arduino, Javier Calvo, Massimo Ritta, Sergio Cabeza, Marta Llobera, David Lembo, Antoni Gaya, Manuela Donalisio
Summary: The study shows that lowering the time and temperature of pasteurization can better preserve the bioactive components of human milk and restore its antiviral activity against cytomegalovirus. This work questions the standard pasteurization method and opens the debate on whether the temperature commonly used in human milk banks should be lowered for better preservation of the biological components of the milk.
PEDIATRIC RESEARCH
(2023)
Article
Microbiology
Azhar S. Sindi, Lisa F. Stinson, Soo Sum Lean, Yit-Heng Chooi, Gabriela E. Leghi, Merryn J. Netting, Mary E. Wlodek, Beverly S. Muhlhausler, Donna T. Geddes, Matthew S. Payne
Summary: This study investigates the impact of maternal dietary intervention during lactation on the infant gut microbiome. The results show that a short-term reduced fat and sugar diet can alter the functional potential of the infant gut microbiome, but not its taxonomy. Changes in maternal protein and sugar intake are correlated with changes in the relative abundances of certain bacterial species.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Pediatrics
Anita L. Esquerra-Zwiers, Carly Mulder, Lauren Czmer, Anastasia Perecki, Emilie Dykstra Goris, Ching Tat Lai, Donna Geddes
Summary: This study investigated the associations between day 10 postpartum secretory activation biomarkers and breastfeeding outcomes. The results showed that elevated sodium levels and sodium: potassium ratio were related to insufficient milk supply, indicating that these biomarkers can be used to identify lactation compromise and improve breastfeeding duration.
JOURNAL OF PEDIATRICS
(2023)
Review
Nutrition & Dietetics
Lisa F. Stinson, Jie Ma, Azhar S. Sindi, Donna T. Geddes
Summary: Human milk microbiome research faces technical challenges such as sample collection, DNA extraction and sequencing, viability, and contamination. This study analyzes various methodological approaches to provide recommendations for robust and reproducible analysis of the human milk microbiome.
Article
Obstetrics & Gynecology
Sharon Lisa Perrella, Emma-Lee Anderton-May, Grace McLoughlin, Ching Tat Lai, Karen Norrie Simmer, Donna Tracy Geddes
Summary: This study found that milk sodium concentration (Na) and sodium:potassium ratio (Na:K) may be useful indicators of breast health. Mastitis can cause a significant decrease in milk production, regardless of the presence of culture-positive infection.
BREASTFEEDING MEDICINE
(2022)
Article
Multidisciplinary Sciences
Azhar S. Sindi, Ali S. Cheema, Michelle L. Trevenen, Donna T. Geddes, Matthew S. Payne, Lisa F. Stinson
Summary: This study investigated the bacterial composition of human milk in an Australian population and its associations with maternal and infant factors. The results showed that the bacterial composition of Australian human milk was influenced by maternal BMI, mode of delivery, breast pump use, and infant pacifier use.
Review
Nutrition & Dietetics
Jie Ma, Debra J. J. Palmer, Donna Geddes, Ching Tat Lai, Lisa Stinson
Summary: Infant growth trajectory may influence later-life obesity, and bioactive components in human milk, such as microbiota, oligosaccharides, fatty acids, and antimicrobial proteins, play a role in infant gut microbiome and metabolism, potentially affecting the development of obesity.
Article
Microbiology
Lisa F. Stinson, Donna T. Geddes, Lucy L. Furfaro
Summary: Human milk is the best nutrition source for infants and contains bioactive compounds and microorganisms. Pasteurization and UV-C irradiation are both used to prevent pathogen transmission, but UV-C may be more effective in eliminating potential phages that could affect infant gut colonization. Further studies are needed to explore the impact of pasteurization methods on bacteriophage titres in human milk.
FEMS MICROBIOLOGY LETTERS
(2023)
Review
Nutrition & Dietetics
Isabella Norrish, Azhar Sindi, Vanessa S. Sakalidis, Ching Tat Lai, Jacki L. McEachran, Mya Thway Tint, Sharon L. Perrella, Mark P. Nicol, Zoya Gridneva, Donna T. Geddes
Summary: Human milk provides essential elements for infant growth and development. Recent studies have found associations between the intake of human milk components and infant body composition, which may explain the reduced risk of childhood obesity among breastfed infants. However, quantifying the infant's actual intake of milk components is crucial for understanding these relationships.
Article
Nanoscience & Nanotechnology
Syaza Y. Binte Abu Y. Bakar, Malinda Salim, Andrew J. Clulow, Susanne Seibt, Cornelia B. Landersdorfer, Donna T. Geddes, Kevin R. Nicholas, Ben J. Boyd
Summary: Colostrum provides essential bioactive components for the infant gut microbiota colonization and preventing infectious diseases. However, substitutes are urgently needed for premature infants when colostrum is unavailable. This study aimed to design an appropriate lipid component for a colostrum substitute and evaluate its ability to reduce inflammation in intestinal cells. Analysis showed that the digestion of colostrum and the substitute mixtures had similar lipid profiles but exhibited subtle differences in self-assembly behavior. Bifidobacteria cultured on the substitute mixtures modulated the biological markers of necrotizing enterocolitis, suggesting the potential of these substitutes in reducing the characteristics associated with this disease.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nutrition & Dietetics
Majed A. Suwaydi, Ching Tat Lai, Alethea Rea, Zoya Gridneva, Sharon L. Perrella, Mary E. Wlodek, Donna T. Geddes
Summary: There is a lack of understanding about the daily variations in hormones and macronutrients in human milk (HM) and inconsistent sample collection protocols between studies. This study investigated these variations by collecting milk samples from 22 lactating women before and after each breastfeed or expression. The results showed variations in hormone and nutrient concentrations, as well as circadian rhythms in milk volume. Standardized and rigorous sampling procedures considering both within-feed and circadian variations are necessary for a precise understanding of the impacts of these components on infant health, growth, and development.
Article
Pediatrics
Vanessa S. Sakalidis, Alethea Rea, Sharon L. Perrella, Jacki McEachran, Grace Collis, Jennifer Miraudo, Stuart A. Prosser, Lisa Y. Gibson, Desiree Silva, Donna T. Geddes
Summary: The COVID-19 pandemic has had an impact on the wellbeing of new mothers and their breastfeeding experience, with restrictions on access to support networks. This study highlights the need for adaptable perinatal services and access to continuous education and clinical care for women experiencing concerns about breastfeeding and maternal wellbeing.
EUROPEAN JOURNAL OF PEDIATRICS
(2022)
Article
Health Care Sciences & Services
Sharon Lisa Perrella, Jennifer Miraudo, Alethea Rea, Donna Tracy Geddes, Stuart Anthony Prosser
Summary: This study evaluated maternal satisfaction with a multidisciplinary team-based maternity care service led by general practitioners with obstetric training (GPOs) and midwives. The results showed that high levels of satisfaction can be achieved in women of low obstetric risk through the provision of GPO-midwife led multidisciplinary care, particularly in emotional care and discussing concerns.
JOURNAL OF PATIENT EXPERIENCE
(2022)
