Article
Soil Science
Gabriela C. Alves, Carlos L. R. Dos Santos, Jerri E. Zilli, Fabio B. Dos Reis Junior, Ivanildo E. Marriel, Farley A. da F. Breda, Robert M. Boddey, Veronica M. Reis
Summary: The study found that the application of H. seropedicae inoculant increased the amount of N in plants due to biological nitrogen fixation, and there is a better chance of yield response to inoculation under low nitrogen fertilizer application in the off-season.
Article
Microbiology
Elisandra Triches da Cunha, Ana Marina Pedrolo, Jessica Cavalheiro Ferreira Bueno, Tomas Pelizzaro Pereira, Claudio Roberto Fonseca Sousa Soares, Ana Carolina Maisonnave Arisi
Summary: This study investigated the colonization and growth-promoting effect of H. seropedicae strain SmR1 on maize in the early stages of development. The results showed that inoculation with H. seropedicae significantly increased the root biomass of maize, indicating its potential as an inoculant in agricultural systems.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Plant Sciences
Luiz Eduardo Souza da Silva Irineu, Cleiton de Paula Soares, Tatiane Sanches Soares, Felipe Astolpho de Almeida, Fabricio Almeida-Silva, Rajesh Kumar Gazara, Carlos Henrique Salvino Gadelha Meneses, Luciano Pasqualoto Canellas, Vanildo Silveira, Thiago Motta Venancio, Fabio Lopes Olivares
Summary: The endophytic bacterium Herbaspirillum seropedicae can promote early growth in maize by increasing nitrogen uptake and assimilation, hormonal regulation, and enhancing carbon-nitrogen metabolism.
Article
Microbiology
Pablo Carril, Joana Cruz, Claudia di Serio, Giuseppe Pieraccini, Sylia Ait Bessai, Rogerio Tenreiro, Cristina Cruz
Summary: This study explored the relationship between seed-borne endophytic bacteria and plant metabolism, revealing that the bacteria can modulate key metabolites and influence plant phenotype, ultimately affecting root growth. Inoculation with bacteria led to changes in the metabolic potential of endophytic communities, indicating a crucial role in regulating plant growth.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Spenser Waller, Stacy L. Wilder, Michael J. Schueller, Alexandra B. Housh, Stephanie Scott, Mary Benoit, Avery Powell, Garren Powell, Richard A. Ferrieri
Summary: Herbaspirillum seropedicae, an endophyte with the potential to promote plant growth and fix nitrogen, relies heavily on plant-borne carbon as its main energy source. Internal root colonization does not shield the microorganisms from the nitrogen environment.
Article
Plant Sciences
Cong van Doan, Tobias Zust, Corina Maurer, Xi Zhang, Ricardo A. R. Machado, Pierre Mateo, Meng Ye, Bernardus C. J. Schimmel, Gaetan Glauser, Christelle A. M. Robert
Summary: The study found that plant leaves exposed to herbivore-induced plant volatiles increase defense mechanisms, but roots do not show the same response. Maize roots exposed to belowground herbivore-induced plant volatiles did not enhance defense traits, indicating that volatile-mediated defense regulation is mainly limited to plant leaves.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Agricultural Engineering
Joseani S. Avila, Joilson S. Ferreira, Rayka K. A. Santos, Pablo A. da Rocha, Maida C. D. de Lima
Summary: This study aimed to evaluate the effects of green manure and inoculation with diazotrophic bacteria on maize yield under an organic cropping system. The association between H. seropedicae and jack bean resulted in a 44.91% increase in grain yield compared to the control, providing a viable alternative for organic maize cultivation.
REVISTA BRASILEIRA DE ENGENHARIA AGRICOLA E AMBIENTAL
(2023)
Article
Soil Science
Kaewagsorn Saowong, Patompong Johns Saengwilai, Marut Fuangarworn, Akihiro Nakamura, Ekgachai Jeratthitikul
Summary: This study investigated the effects of different maize varieties and root traits on the diversity and community composition of soil arthropods. The findings highlight the importance of root traits, especially the angle of the roots, in enhancing arthropod biodiversity in the rhizosphere ecosystem.
APPLIED SOIL ECOLOGY
(2022)
Article
Environmental Sciences
Hao Zhang, Yingying Qian, Dandan Fan, Yanning Tian, Xing Huang
Summary: Root exudates and their components, such as organic acids, can induce the biosynthesis of biofilms to promote BSM degradation, emphasizing the contribution of root biofilms in reducing BSM damage to maize.
ENVIRONMENTAL POLLUTION
(2022)
Article
Agronomy
Guixin Zhang, Shibo Zhang, Zhenqing Xia, Mengke Wu, Jingxuan Bai, Haidong Lu
Summary: With the increased use of agricultural film, the potential environmental risks have been getting attention. A two-year field experiment in China's Loess Plateau region studied the effects of residual biodegradable film and polyethylene film on soil moisture, maize root, and productivity at different residual levels.
Article
Agronomy
Dan Liao, Chaochun Zhang, Hans Lambers, Fusuo Zhang
Summary: This study investigated the effects of root residues from different species combinations in sole cropping or intercropping systems on soil phosphorus (P) pools. The results showed that adding intercrop root residues increased microbial biomass and acid phosphatase activity, accelerating organic P mineralization and increasing biomass and total P content in a P-deficient soil.
Article
Biotechnology & Applied Microbiology
Marcos Rodrigues Maldonado, Robson Carlos Alnoch, Janaina Marques de Almeida, Leandro Alves dos Santos, Andressa Tedesco Andretta, Rocio del Pilar Cuaspa Ropain, Emanuel Maltempi de Souza, David Alexander Mitchell, Nadia Krieger
Summary: There is a need for systematic evaluation to identify positions in the protein molecule for engineering lipases with desired selectivities. Based on representative studies, key sites of the catalytic cleft for mutations to improve enantioselectivity have been identified, depending on the type of chiral substrate.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Microbiology
Raul Matias Cezar, Fabiane Machado Vezzani, Glaciela Kaschuk, Eduardo Balsanelli, Emanuel Maltempi de Souza, Luciano Kayser Vargas, Rudimar Molin
Summary: Crop diversity can affect the frequencies of soil microbial communities and alter major soil functions, with crop rotation system potentially reducing the frequencies of certain anaerobic metabolism bacteria. However, crop succession does not significantly impact the richness and diversity indexes of soil bacteria and archaea.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Diogo Henrique Kita, Gisele Alves de Andrade, Juliana Morais Missina, Kahoana Postal, Viktor Kalbermatter Boell, Francielli Sousa Santana, Ingrid Fatima Zattoni, Isadora da Silva Zanzarini, Vivian Rotuno Moure, Fabiane Gomes de Moraes Rego, Geraldo Picheth, Emanuel Maltempi de Souza, David A. Mitchell, Suresh Ambudkar, Giovana Gioppo Nunes, Glaucio Valdameri
Summary: The study found that polyoxovanadates can serve as inhibitors of ABC drug transporters, selectively inhibiting P-glycoprotein with V-10 and V-18 being the most promising compounds.
Article
Soil Science
Eliane Cristina Gruszka Vendruscolo, Dany Mesa, Emanuel Maltempi de Souza
Summary: The study evaluated the impact of no-tillage and fallow systems on soil microbiota. It found that the rhizosphere microbial community diversity was significantly lower in the no-tillage system compared to the fallow system, with each having unique microbial populations.
APPLIED SOIL ECOLOGY
(2022)
Article
Virology
Douglas Adamoski, Valter Antonio de Baura, Ana Carolina Rodrigues, Carla Adriane Royer, Mateus Nobrega Aoki, Marcel Kruchelski Tscha, Ana Claudia Bonatto, Roseli Wassem, Meri Bordignon Nogueira, Sonia Mara Raboni, Bernardo Montesanti Machado de Almeida, Edvaldo da Silva Trindade, Daniela Fiori Gradia, Emanuel Maltempi Souza, Jaqueline Carvalho de Oliveira
Summary: Screening efforts and genomic surveillance are crucial for evaluating the progress of the COVID-19 pandemic. A study in Curitiba, Brazil analyzed COVID-19 cases, conducted diagnosis and genotyping, and found that the Omicron variant quickly became dominant. Vaccination was associated with reduced mortality rates.
Article
Biochemistry & Molecular Biology
Cleiton de Paula Soares, Michelle Zibetti Trada-Sfeir, Leonardo Araujo Terra, Jessica de Paula Ferreira, Carlos Magno Dos-Santos, Izamara Gesiele Bezerra de Oliveira, Jean Luiz Simoes Araujo, Carlos Henrique Salvino Gadelha Meneses, Emanuel Maltempi de Souza, Jose Ivo Baldani, Marcia Soares Vidal
Summary: This study provides a comprehensive analysis of the early transcriptional response of Gluconacetobacter diazotrophicus to iron limitations, revealing the regulatory mechanisms of genes related to iron homeostasis and identifying genes that are overexpressed under iron-limited conditions. Additionally, the study demonstrates that the fur protein of G. diazotrophicus can complement an E. coli fur mutant.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Anahi Coniglio, Giovanni Larama, Romina Molina, Veronica Mora, Daniela Torres, Anelis Marin, Andres Ignacio Avila, Coline Lede NoirCarlan, Leonardo Erijman, Eva Lucia Figuerola, Emanuel Maltempi de Souza, Fabricio Cassan
Summary: Azospirillum is a crucial plant growth promoting rhizobacteria used in global agriculture. This study reveals that Azospirillum inoculation can alter the microbial community structure in maize rhizosphere, resulting in an increase in the relative abundance of beneficial bacterial genera.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2022)
Article
Chemistry, Analytical
Gustavo Martins, Jeferson L. Gogola, Lucas H. Budni, Mauricio A. Papi, Maritza A. T. Bom, Maria L. T. Budel, Emanuel M. de Souza, Marcelo Muller-Santos, Breno C. B. Beirao, Craig E. Banks, Luiz H. Marcolino-Junior, Marcio F. Bergamini
Summary: The present work reports an innovative approach for constructing immunosensors using graphene quantum dots and polyhydroxybutyric acid modified carbon electrodes. This approach allows for the detection of anti-S antibodies, showing promising results in terms of selectivity and sensitivity. The proposed sensor has the potential to be developed into a single-drop immunosensor.
ANALYTICA CHIMICA ACTA
(2022)
Article
Multidisciplinary Sciences
Regis Goulart Rosa, Julia Spinardi, Kristen E. Allen, Joselia Manfio, Cintia Laura Pereira de Araujo, Mirian Cohen, Caroline Cabral Robinson, Daniel Sganzerla, Diogo Ferreira, Emanuel Maltempi de Souza, Jaqueline Carvalho de Oliveira, Daniela Fiori Gradia, Ana Paula Carneiro Brandalize, Gabriela Almeida Kucharski, Fernando Pedrotti, Cristina de Oliveira Rodrigues, Moe H. Kyaw, Graciela Del Carmen Morales Castillo, Amit Srivastava, John M. McLaughlin, Maicon Falavigna
Summary: This study aims to evaluate the effectiveness of the Pfizer-BioNTech BNT162b2 vaccine against symptomatic COVID-19 and related complications in a real-world setting in Brazil. A case-control study is being conducted among patients seeking care in the public health system, with follow-up and regular telephone interviews. The study findings will be disseminated through peer-reviewed publications and conference presentations.
Article
Biochemical Research Methods
Erick Parize, Edileusa C. M. Gerhardt, Ana C. M. Oliveira, Fabio O. Pedrosa, Emanuel M. Souza, Luciano F. Huergo, Maria B. R. Steffens
Summary: The study successfully heterologously expressed and purified the Rho protein from the diazotrophic environmental bacterium Azospirillum brasilense, showing its similarity to the E. coli orthologue. The purified AbRho protein exhibited correctly folded and active characteristics, indicating it is a RNA-dependent NTPase enzyme.
PROTEIN EXPRESSION AND PURIFICATION
(2022)
Article
Microbiology
Camila P. Perico, Camilla R. De Pierri, Giuseppe Pasqualato Neto, Danrley R. Fernandes, Fabio O. Pedrosa, Emanuel M. de Souza, Roberto T. Raittz
Summary: This study tracked the development of the COVID-19 pandemic in Brazil from a genomic/proteomic perspective and analyzed the comparative and evolutionary panorama of the lineages in the country. The study found two different genetic profiles of the epidemics in Brazil, with the emergence of the more aggressive P.1 lineages in the second wave. The unbalanced sequencing among Brazilian states may have facilitated the silent entry and dissemination of P.1 and other dangerous variants.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Mycology
Nickolas Menezes da Silva, Guilherme Fonseca Reis, Flavia de Fatima Costa, Maria Eduarda Grisolia, Marlon Roger Geraldo, Bruno Paulo Rodrigues Lustosa, Bruna Jacomel Favoreto de Souza Lima, Vinicius Almir Weiss, Emanuel Maltempi de Souza, Ruoyu Li, Yinggai Song, Mariana Machado Fidelis Nascimento, Diogo Robl, Renata Rodrigues Gomes, G. Sybren de Hoog, Vania Aparecida Vicente
Summary: This study focused on the sequencing, assembly, and description of the whole genome of Cladophialophora exuberans, a black yeast related fungus, with an emphasis on genes and pathways related to carbon and toxin management, tolerance and bioremediation of lead and copper, and metal homeostasis. The results showed that the strain exhibited growth inhibition at minimum inhibitory and fungicidal concentrations of copper and lead, and could grow at higher concentrations in agar tests. In addition, the strain demonstrated uptake capacities of copper and lead in heavy metal bioremediation assays. This study contributed to the annotation of genes involved in heavy metal homeostasis and provided insights into the mechanisms of tolerance and adaptation to extreme conditions.
Article
Chemistry, Analytical
Gustavo Martins, Helena R. Galeski, Gabrielle A. Andrade, Marcia G. P. Valenga, Maria K. Ramos, Aldo J. G. Zarbin, Bruno C. Janegitz, Marcelo Mueller-Santos, Emanuel M. de Souza, Luiz Humberto Marcolino-Junior, Marcio F. Bergamini
Summary: Researchers proposed a new method for assembling an electrochemical immunosensor based on a one-step approach for selective layer formation, which is crucial for rapid and reliable diagnostic tests for new viruses and diseases. This method successfully detected SARS-CoV-2 nucleocapsid protein in different matrices with low detectable values, and demonstrated selectivity against interferents. The proposed assembly strategy reduces cost, analysis time, and waste generation, making it a suitable and reliable alternative for healthcare diagnostics.
ANALYTICA CHIMICA ACTA
(2023)
Article
Soil Science
Jessica Pereira de Souza, Fabiane Machado Vezzani, Tatiana Suzin Lazeris, Glaciela Kaschuk, Emanuel Maltempi de Souza, Eduardo Balsanelli, Deborah Pinheiro Dick, Helio Antonio Wood Joris, Cimelio Bayer
Summary: Crop system plays a crucial role in shaping soil microbial communities and organic matter content. This study investigates the long-term effects of different crop systems on soil composition and microbial diversity in subtropical soil. The results show that the current crop species significantly influence microbial community structure and diversity, while the long-term history of crop rotation has a relatively smaller impact. Crop rotation increases the proportion of carbohydrate-derived organic matter, while crop succession increases the proportion of alkyl-C and N/O alkyl-C. Crop species richness in rotation maintains soil functions and enhances the formation of biochemically labile organic matter, contributing to soil ecosystem functioning.
SOIL & TILLAGE RESEARCH
(2023)
Article
Environmental Sciences
Ricardo Belmonte-Lopes, Carlos E. R. Barquilha, Caroline Kozak, Demian S. Barcellos, Barbara Z. Leite, Fernanda J. O. Gomes da Costa, William L. Martins, Pamela E. Oliveira, Edy H. R. A. Pereira, Cesar R. Mota Filho, Emanuel M. de Souza, Gustavo R. C. Possetti, Vania A. Vicente, Ramiro G. Etchepare
Summary: The COVID-19 pandemic led to the collapse of healthcare systems and the emergence of wastewater-based epidemiology. This study conducted SARS-CoV-2 wastewater surveillance in Curitiba, Brazil, and found a correlation between viral loads and reported cases. The approach used proved to be a robust early warning system that can aid public decision-making and health interventions.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)