Review
Biotechnology & Applied Microbiology
Dominic R. Whittall, Katherine Baker, Rainer Breitling, Eriko Takano
Summary: Spider silk is known for its impressive mechanical properties, but farming spiders for silk is impractical. Research focuses on producing recombinant spider silk proteins in more feasible hosts, with the goal of developing a highly efficient and cost-effective production system. Genes encoding these proteins have been successfully expressed in various host platforms, showing potential for large-scale production.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Oranicha Hanittinan, Kaewta Rattanapisit, Ashwini Malla, Kittipan Tharakhet, Chutitorn Ketloy, Eakachai Prompetchara, Waranyoo Phoolcharoen
Summary: This study successfully utilized a plant expression system to produce anti-human IgE monoclonal antibodies with comparable functionality to commercial Omalizumab. Although further optimization is needed for antibody yield and purification process, the results demonstrate the promising potential of plant expression system as an effective method for Omalizumab production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Meenakshi Venkatesan, Cameron Semper, Stig Skrivergaard, Rosa Di Leo, Nathalie Mesa, Martin Kroyer Rasmussen, Jette Feveile Young, Margrethe Therkildsen, Peter J. Stogios, Alexei Savchenko
Summary: This study presents a cost-effective method for producing functionally active GFs in Escherichia coli, which can significantly reduce the cost of cell culture medium and facilitate the production of cell-cultured meat and tissue engineering.
Review
Biotechnology & Applied Microbiology
Anna Tippelt, Markus Nett
Summary: Saccharomyces cerevisiae is a widely used host organism in biotechnology for producing a variety of compounds, from vaccines to complex natural products like polyketides and nonribosomal peptides. Efforts have been made to reconstitute multi-step catalytic processes for these compounds in yeast, requiring functional expression of enzymes and protection of the host from toxicity.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Ryota Kurashiki, Kosuke Koyama, Yukina Sakaguchi, Yuta Okumura, Takashi Ohshiro, Hirokazu Suzuki
Summary: Through studying Geobacillus kaustophilus HTA426, we discovered a robust promoter for efficient recombinant protein production at high temperatures, and successfully applied it to other strains with satisfactory yields. Additionally, we developed a simple purification method for extracting recombinant proteins from both intracellular and extracellular fractions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Yann Waltenspuhl, Jeliazko R. Jeliazkov, Lutz Kummer, Andreas Pluckthun
Summary: This study presents an engineering strategy to improve the properties of challenging GPCRs by combining three directed evolution methods. Utilizing a Next-Generation Sequencing (NGS) strategy, the study successfully selected improved variants of the human oxytocin receptor and compared mutations in different hosts. This research provides insights into the evolutionary pressure on the same membrane protein in prokaryotes and eukaryotes and offers a general methodology for accurate analysis of point mutants during directed evolution.
SCIENTIFIC REPORTS
(2021)
Review
Biotechnology & Applied Microbiology
Ramon Roca-Pinilla, Leszek Lisowski, Anna Aris, Elena Garcia-Fruitos
Summary: Host defense peptides (HDPs) are small proteins with potent antibacterial and immunomodulatory activities, suitable for clinical applications. Traditional methods of producing antimicrobial peptides are expensive and toxic, making recombinant production of HDPs an alternative. This review explores different strategies for optimizing HDPs, including fine-tuning their activities, bioengineering, and recombinant production in various cell factories.
MICROBIAL CELL FACTORIES
(2022)
Article
Multidisciplinary Sciences
Anthony J. Berndt, Tressa N. Smalley, Bijie Ren, Ryan Simkovsky, Amr Badary, Ashley E. Sproles, Francis J. Fields, Yasin Torres-Tiji, Vanessa Heredia, Stephen P. Mayfield
Summary: Recombinant production of the SARS-CoV-2 spike protein Receptor Binding Domain (RBD) in green algae Chlamydomonas has shown promising results, with the produced proteins having similar activity to those produced in mammalian cells. This low-cost alternative may be beneficial due to the large-scale, inexpensive cultivation of algae.
Article
Plant Sciences
Shi-Jian Song, Hai-Ping Diao, Byeongho Moon, Areum Yun, Inhwan Hwang
Summary: Plants are a cost-effective platform for recombinant production and have the advantage of low risk of contamination of human pathogens. This study demonstrates the versatility of the B1 domain of Streptococcal protein G (GB1) as a fusion tag for recombinant protein production in plants. Fusion with the GB1 domain increases expression levels and stability of fusion proteins, while direct detection using secondary anti-IgG antibody simplifies analysis. Therefore, the small GB1 domain can be used as a versatile tag for recombinant protein production in plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemical Research Methods
Anirban Banerjee, Valerie Ward
Summary: Microalgae is a promising platform for producing therapeutic recombinant proteins, which can be used as edible vaccines or drug delivery vehicles, in addition to their natural abundance as nutraceutical products.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Review
Engineering, Chemical
Ace Bryan Sotelo Cabal, Tzong-Yuan Wu
Summary: This review article discusses the devastation caused by coronaviruses in both human and animal populations and emphasizes the importance of science-based approaches, particularly biotechnology, in overcoming the health and economic threats posed by coronaviruses. The article provides an overview of the genome, morphology, and key structural proteins of different coronaviruses and highlights the various recombinant protein technology systems used for the development of countermeasures against coronaviruses.
Review
Microbiology
Sara Restrepo-Pineda, Nestor O. Perez, Norma A. Valdez-Cruz, Mauricio A. Trujillo-Roldan
Summary: The production of recombinant proteins from Escherichia coli using a thermoinducible expression system has been extensively studied to increase product yields. This system avoids the use of chemical inducers, minimizing contamination risks, and knowledge derived from the regulatory elements of bacteriophage lambda has contributed to improving bioprocesses using this system. Metabolic and physiological changes in host cells caused by heat stress and overproduction of recombinant proteins are described, along with discussions on the current status and future applications of systems using heat to induce protein production.
FEMS MICROBIOLOGY REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Bikash K. Bhandari, Chun Shen Lim, Paul P. Gardner
Summary: Experiments planned using accurate prediction algorithms can reduce failures in recombinant protein production. TISIGNER offers three web services specialized in synonymous optimization, solubility, and signal peptide analysis, which are interconnected for users to switch between when optimizing genes of interest.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Engineering, Civil
Ashraf Abosekeen, Umar Iqbal, Aboelmagd Noureldin, Michael J. Korenberg
Summary: This research focuses on developing an integrated multi-sensor POS/NAV system to improve the positioning accuracy of autonomous land vehicles, and has achieved significant performance improvement by introducing adaptive cruise control frequency modulated continuous wave radar, magnetometer, and reduced inertial sensor system to address satellite signal blockage.
IEEE TRANSACTIONS ON INTELLIGENT TRANSPORTATION SYSTEMS
(2021)
Article
Biochemical Research Methods
Joanne Hothersall, Rita E. Godfrey, Christos Fanitsios, Tim W. Overton, Stephen J. W. Busby, Douglas F. Browning
Summary: By constructing a suite of bacterial promoters with different strengths, it is possible to achieve flexible expression of target proteins in E. coli, with the ability to adjust expression levels as needed, suitable for various culture conditions and E. coli strains.
Article
Clinical Neurology
Gudrun Schottmann, Akosua Sarpong, Carmen Lorenz, Natalie Weinhold, Esther Gill, Lisa Teschner, Sacha Ferdinandusse, Ronald J. A. Wanders, Alessandro Prigione, Markus Schuelke
MOVEMENT DISORDERS
(2016)
Review
Cell Biology
Barbara Mlody, Carmen Lorenz, Gizem Inak, Alessandro Prigione
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2016)
Review
Cell & Tissue Engineering
Gizem Inak, Carmen Lorenz, Pawel Lisowski, Annika Zink, Barbara Mlody, Alessandro Prigione
Article
Cell & Tissue Engineering
Carmen Lorenz, Annika Zink, Marie-Therese Henke, Selma Staege, Barbara Mlody, Miriam Buenning, Erich Wanker, Sebastian Diecke, Markus Schuelke, Alessandro Prigione
Summary: In this study, we successfully generated four human iPSC lines from fibroblasts of four patients with maternally inherited Leigh syndrome (MILS). These iPSC lines retained the homoplasmic mutations of the parental fibroblasts and exhibited pluripotency characteristics.
STEM CELL RESEARCH
(2022)
Article
Cell & Tissue Engineering
Carmen Lorenz, Pierre Lesimple, Raul Bukowiecki, Annika Zink, Gizem Inak, Barbara Mlody, Manvendra Singh, Marcus Semtner, Nancy Mah, Karine Aure, Megan Leong, Oleksandr Zabiegalov, Ekaterini-Maria Lyras, Vanessa Pfiffer, Beatrix Fauler, Jenny Eichhorst, Burkhard Wiesner, Norbert Huebner, Josef Priller, Thorsten Mielke, David Meierhofer, Zsuzsanna Izsvak, Jochen C. Meier, Frederic Bouillaud, James Adjaye, Markus Schuelke, Erich E. Wanker, Anne Lombes, Alessandro Prigione