Article
Biotechnology & Applied Microbiology
Jung-Hoon Bae, Mi-Jin Kim, Bong Hyun Sung, Yong-Su Jin, Jung-Hoon Sohn
Summary: The extracellular conversion of xylose to xylulose before uptake has been found to be effective in xylose fermentation or glucose/xylose co-fermentation, reducing the competition between glucose and xylose for cellular uptake. The engineered XI secretion system developed in this study may enable the simultaneous utilization of C5/C6 sugars from sustainable lignocellulosic biomass.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agronomy
Heeyoung Park, Sang Un Park, Byeong-Kwan Jang, Jeong Jae Lee, Yong Suk Chung
Summary: Kenaf, an annual fiber crop mainly grown in India and China, is emerging as a promising bio-based energy source due to its rapid growth, CO2 absorption capabilities, and high productivity. The study evaluated 10 different cultivars of kenaf for cellulosic ethanol production, finding that ethanol yield and productivity were significantly impacted by cultivar type, rather than biomass composition. Engineering yeast to utilize xylose under pH control can maximize ethanol production from kenaf.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2021)
Article
Computer Science, Information Systems
Xin-Chi Shi, Yan Zhang, Ting Wang, Xiang-Chen Wang, Hai-Bin Lv, Pedro Laborda, Ting-Ting Duan
Summary: This study investigated the potential utilization of xylose using bioengineered Saccharomyces cerevisiae strains. It was found that driving xylitol dehydrogenase (XDH) gene expression with the PGK1 promoter improved xylose utilization and xylitol production. Overexpression of xylulokinase and XRIXDH from Pichia stipitis decreased xylitol accumulation and increased ethanol production.
IEEE JOURNAL OF BIOMEDICAL AND HEALTH INFORMATICS
(2022)
Article
Biotechnology & Applied Microbiology
Bo Li, Li Wang, Jin-Yu Xie, Zi-Yuan Xia, Cai-Yun Xie, Yue-Qin Tang
Summary: This study revealed the regulatory mechanisms of Haa1p and Tye7p in improving the fermentation performance and inhibitor tolerance of S. cerevisiae through comparative transcriptomic analyses.
MICROBIAL CELL FACTORIES
(2022)
Review
Biotechnology & Applied Microbiology
Juhi Sharma, Vinod Kumar, Rajendra Prasad, Naseem A. Gaur
Summary: Lignocellulosic biomass is an abundant material on earth and can be processed by microorganisms to produce biofuels. Saccharomyces cerevisiae is an ideal candidate for consolidated bio-processing (CBP) to produce cellulose-based ethanol. However, there are still challenges in the process, such as incomplete substrate catabolism and low expression of heterologous proteins.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Energy & Fuels
Lucia Coimbra, Karen Malan, Alejandra Fagundez, Mairan Guigou, Claudia Lareo, Belen Fernandez, Martin Pratto, Silvia Batista
Summary: Genetically engineered yeast strains were developed for efficient ethanol production from lignocellulosic biomass. The recombinant strain CAT-1-XIT (pRS42K::XI) showed promising results with 74% consumption of D-xylose and production of 12.6 g/L ethanol.
BIOENERGY RESEARCH
(2023)
Article
Agricultural Engineering
Meilin Kong, Xiaowei Li, Tongtong Li, Xuebing Zhao, Mingjie Jin, Xin Zhou, Hanqi Gu, Vladimir Mrsa, Wei Xiao, Limin Cao
Summary: CCW12(OE) is a Saccharomyces cerevisiae strain constructed by overexpressing CCW12, which shows high ethanol production efficiency in various hydrolysates and exhibits better stress resistance and fermentation performance compared to the control strain WXY70.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Viviani Tadioto, Junior Romeo Deoti, Caroline Muller, Bruna Raquel de Souza, Odinei Fogolari, Marcela Purificacao, Anderson Giehl, Leticia Deoti, Ana Carolina Lucaroni, Akinori Matsushika, Helen Treichel, Boris Ugarte Stambuk, Sergio Luiz Alves Junior
Summary: This study analyzed the effect of a recently discovered wild yeast strain, UFFS-CE-3.1.2, on ethanol production. The results showed that the strain was not significantly affected by furfural and NaCl, while acetic acid negatively affected glucose consumption. The pH positively influenced all the analyzed responses. Sugar concentration had a negative impact on ethanol, xylitol, and cellular biomass yields. A genetically engineered strain, JDY-01, showed higher xylose consumption and ethanol production, as well as better tolerance to acetic acid.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Yixuan Zhu, Jingtao Zhang, Lang Zhu, Zefang Jia, Qi Li, Wei Xiao, Limin Cao
Summary: Efficient xylose fermentation in budding yeast was achieved through rational promoter elements engineering, with HXT7 showing the best performance among surveyed promoters. The redox balance of the xylose utilization pathway was optimized to achieve a balanced xylose metabolism toward ethanol formation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biochemical Research Methods
Liang Sun, Yong-Su Jin
Summary: The microbial conversion of plant biomass into fuels and chemicals using engineered yeast has shown progress in efficiently converting xylose and co-utilizing it with glucose. Research investments have facilitated the simultaneous fermentation of xylose and glucose, with attention now turning towards improving xylose-utilizing efficiency and achieving robust co-fermentation under industrial conditions. Harnessing the advantageous traits of yeast xylose metabolism is expected to lead to the production of diverse fuels and chemicals.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Green & Sustainable Science & Technology
Boyang He, Bo Hao, Haizhong Yu, Fen Tu, Xiaoyang Wei, Ke Xiong, Yajun Zeng, Hu Zeng, Peng Liu, Yuanyuan Tu, Yanting Wang, Heng Kang, Liangcai Peng, Tao Xia
Summary: This study demonstrates an effective yeast-engineering approach for efficient xylose utilization and provides a powerful strategy for enhancing bioethanol production in bioenergy crops.
Article
Microbiology
Cheng Cheng, Wei-Bin Wang, Meng-Lin Sun, Rui-Qi Tang, Long Bai, Hal S. Alper, Xin-Qing Zhao
Summary: This study reveals that the NGG1 gene in yeast plays a regulatory role in xylose metabolism, and its deletion can improve xylose consumption. Further analysis shows that the deletion of NGG1 also affects mitochondrial function, ATP biosynthesis, and amino acid accumulation. These findings provide a basis for developing yeast strains for the production of ethanol and biochemicals from lignocellulosic biomass.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agricultural Engineering
Yufeng Xie, Lijun Qin, Degang Zhao
Summary: Efficient extraction of gutta-percha from Eucommia ulmoides leaves using enzymatic treatment with cellulase was achieved. The pretreated leaves showed the highest extraction rate (2.35%) and good thermal stability, with gutta-percha exhibiting an α-crystal type structure.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agriculture, Multidisciplinary
Biying Wang, Xiaoya Zhao, Tong Fu, Xiaoyi Chen, Xiaoyu Guo, Xianzhen Li, Fan Yang
Summary: A positively evolved Suc2 promoter (SUC 2p) with stronger promoter activity than the wild-type Suc2 promoter (SUC 2wtp) was obtained in this study. The strength of SUC 2p was modulated by different glucose concentrations, with significantly enhanced promoter activity at low glucose concentrations.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Yeon-Jung Lee, Phuong Hoang Nguyen Tran, Ja Kyong Ko, Gyeongtaek Gong, Youngsoon Um, Sung Ok Han, Sun-Mi Lee
Summary: Efficient xylose catabolism in engineered Saccharomyces cerevisiae enables the production of fuels and chemicals derived from acetyl-CoA through glucose/xylose co-fermentation. Introduction of an n-butanol-biosynthesis pathway in a glucose/xylose co-fermenting S. cerevisiae strain resulted in higher n-butanol production, which was explained by increased acetyl-CoA and NADPH pools. Acetate supplementation further increased n-butanol production and was validated in fermentation of lignocellulosic hydrolysates containing acetate.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Benjarat Bunterngsook, Thanaporn Laothanachareon, Chayanon Chotirotsukon, Hiroyuki Inoue, Tatsuya Fujii, Tamotsu Hoshino, Niran Roongsawang, Sanchai Kuboon, Wasawat Kraithong, Wikanda Techanan, Natthakorn Kraikul, Verawat Champreda
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2018)
Article
Biochemistry & Molecular Biology
Yusuke Nakamichi, Thierry Fouquet, Shotaro Ito, Masahiro Watanabe, Akinori Matsushika, Hiroyuki Inoue
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemical Research Methods
Thierry Fouquet, Hiroaki Sato, Yusuke Nakamichi, Akinori Matsushika, Hiroyuki Inoue
JOURNAL OF MASS SPECTROMETRY
(2019)
Article
Biotechnology & Applied Microbiology
Yusuke Nakamichi, Thierry Fouquet, Shotaro Ito, Akinori Matsushika, Hiroyuki Inoue
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Microbiology
Hironaga Akita, Akinori Matsushika, Zen-ichiro Kimura
Article
Biochemistry & Molecular Biology
Tatsuya Fujii, Akinori Matsushika
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Keisuke Wada, Tatsuya Fujii, Hironaga Akita, Akinori Matsushika
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Toshihiro Suzuki, Tamotsu Hoshino, Akinori Matsushika
ENZYME AND MICROBIAL TECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Yusuke Nakamichi, Masahiro Watanabe, Akinori Matsushika, Hiroyuki Inoue
Article
Microbiology
Hironaga Akita, Yusuke Nakamichi, Tomotake Morita, Akinori Matsushika
Article
Biochemistry & Molecular Biology
Tatsuya Fujii, Hiroyuki Inoue, Akinori Matsushika
Summary: The study focused on the function of the tclB2 gene in mannanolytic enzyme production by the filamentous fungus Talaromyces cellulolyticus. Results showed that disrupting tclB2 led to decreased production of enzymes, while overexpressing tclB2 resulted in higher enzyme production, suggesting that TclB2 plays a role in mannanolytic enzyme production in T. cellulolyticus.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Motoaki Tojo, Natsumi Fujii, Hironori Yagi, Yuki Yamashita, Katsuyuki Tokura, Kenichi Kida, Akiho Hakoda, Maria-Luz Herrero, Tamotsu Hoshino, Masaki Uchida
Summary: This study investigated the species diversity of Globisporangium spp. in Sanionia moss in Ny-angstrom lesund, Spitsbergen Is., Norway, revealing a unique composition with most species reducing their population over the recent decade.
Article
Microbiology
Seiichi Fujiu, Masanobu Ito, Eriko Kobayashi, Yuichi Hanada, Midori Yoshida, Sakae Kudoh, Tamotsu Hoshino
Summary: The basidiomycetous yeast, Glaciozyma antarctica, was found to produce large amounts of extracellular polysaccharides and a small quantity of ice-binding proteins. It forms frost-columnar colonies in frozen environments and accumulates solutes in unfrozen water within the colonies. The ice-binding proteins produced by the yeast help maintain an unfrozen state of water in the medium after accumulation in the frost-columnar colony.
Article
Biotechnology & Applied Microbiology
Viviani Tadioto, Junior Romeo Deoti, Caroline Muller, Bruna Raquel de Souza, Odinei Fogolari, Marcela Purificacao, Anderson Giehl, Leticia Deoti, Ana Carolina Lucaroni, Akinori Matsushika, Helen Treichel, Boris Ugarte Stambuk, Sergio Luiz Alves Junior
Summary: This study analyzed the effect of a recently discovered wild yeast strain, UFFS-CE-3.1.2, on ethanol production. The results showed that the strain was not significantly affected by furfural and NaCl, while acetic acid negatively affected glucose consumption. The pH positively influenced all the analyzed responses. Sugar concentration had a negative impact on ethanol, xylitol, and cellular biomass yields. A genetically engineered strain, JDY-01, showed higher xylose consumption and ethanol production, as well as better tolerance to acetic acid.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Microbiology
Tamotsu Hoshino, Yuka Yajima, Yosuke Degawa, Atsushi Kume, Oleg B. Tkachenko, Naoyuki Matsumoto
Summary: The fungi in the genera Typhula and Pistillaria from the family Typhulaceae are capable of developing basidiomata from sclerotia or basidia. This diversity in their life cycle suggests a plasticity in response to different growth environments.