Article
Microbiology
Jessica G. Tague, Abish Regmi, Gwendolyn J. Gregory, E. Fidelma Boyd
Summary: Factor for inversion stimulation (Fis) positively regulates the expression of qrr sRNAs in V. parahaemolyticus, controlling swarming motility indirectly through the QS pathway and directly through the surface sensing pathway.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Megha Shah, Veronique L. Taylor, Diane Bona, Yvonne Tsao, Sabrina Y. Stanley, Sheila M. Pimentel-Elardo, Matthew McCallum, Joseph Bondy-Denomy, P. Lynne Howell, Justin R. Nodwell, Alan R. Davidson, Trevor F. Moraes, Karen L. Maxwell
Summary: The study identified a new phage anti-activator protein, Aqs1, which inhibits phage infection by suppressing the quorum-sensing master regulator LasR. Aqs1 also inhibits the bacterial type IV pilus assembly ATPase protein PilB, preventing phages from superinfecting host cells. This research highlights the potential of small phage proteins to disrupt key biological pathways and offers insights into the evolutionary dynamics between phages and their hosts.
Review
Chemistry, Medicinal
Qiaoqiang Li, Shen Mao, Hong Wang, Xinyi Ye
Summary: The survival pressure caused by antibiotics prompts bacteria to develop drug resistance. Quorum sensing is an intercellular communication system that regulates bacterial virulence and biofilm formation. In Pseudomonas aeruginosa, the formation and diffusion of biofilm is closely related to the quorum sensing system. Quorum-sensing inhibitors can reduce bacterial toxicity and enhance sensitivity to antibiotics, making them a popular topic for research and development in anti-infection. This review provides an overview of the Pseudomonas aeruginosa quorum-sensing system and quorum-sensing inhibitors research, which will aid in the future development of novel inhibitors.
Article
Plant Sciences
Tolis Panayi, Yiannis Sarigiannis, Elena Mourelatou, Evroula Hapeshis, Christos Papaneophytou
Summary: This study investigated the anti-quorum sensing (QS) potential of ethanolic extracts of eight aromatic plants from Cyprus. Three of the tested extracts were found to be the most potent inhibitors of AI-2 signaling in bacteria and also inhibited biofilm formation and motility in a concentration-dependent manner. These extracts could serve as a source for identifying novel anti-QS inhibitors to treat antibiotic-resistant infectious diseases.
Article
Biotechnology & Applied Microbiology
Xinrui Zhao, Congjuan Xu, Junze Qu, Yongxin Jin, Fang Bai, Zhihui Cheng, Weihui Wu, Xiaolei Pan
Summary: A study has discovered that a phosphate transporter protein (PitA) in Pseudomonas aeruginosa regulates the expression of T6SSs and bacterial invasion. This research reveals a novel regulatory pathway that senses phosphate limitation and controls bacterial virulence factors.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Kayeen Vadakkan, Ajit Kumar Ngangbam, Kuppusamy Sathishkumar, Nelson Pynadathu Rumjit, Meena Kochappan Cheruvathur
Summary: Pseudomonas aeruginosa, a competitive and biofilm organism with complex quorum systems, poses a significant threat to the medical industry. Inhibiting quorum sensing could be a potential strategy for treating bacterial infections and developing anti-virulent tools.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Multidisciplinary Sciences
Parker Smith, Martin Schuster
Summary: Quorum sensing is a widespread cell density-dependent signaling mechanism in bacteria. This study demonstrates the role of antiactivators in preventing self-sensing in the quorum sensing system of Pseudomonas aeruginosa.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
An Margarete Tan Lim, Glenn G. Oyong, Maria Carmen S. Tan, Chien Chang Shen, Consolacion Y. Ragasa, Esperanza C. Cabrera
Summary: The study found that purified compounds extracted from Andrographis paniculata have inhibitory effects on the quorum sensing-mediated virulence mechanisms of metallo-beta-lactamase (MβL)-producing Pseudomonas aeruginosa, by reducing biofilm formation, protease production, swarming motility, and downregulating the expression of the lasR gene.
Review
Chemistry, Analytical
Yingying Li, Yang Hu, Tao Chen, Yan Chen, Yi Li, Haibo Zhou, Danting Yang
Summary: This review discusses and summarizes advanced detection and sensing strategies for P. aeruginosa cells and its associated quorum sensing molecules over the past ten years. The aim is to achieve rapid, sensitive, and cost-effective diagnosis for infection. The principles of commonly used sensing strategies are briefly introduced, followed by the advancement of these techniques in detecting P. aeruginosa cells and quorum sensing biomarkers. Additionally, the integration of novel platforms for clinical application is highlighted. The current achievements are summarized, and challenges and prospects for the future are proposed.
Article
Microbiology
Shouyi Li, Xuetao Gong, Liwen Yin, Xiaolei Pan, Yongxin Jin, Fang Bai, Zhihui Cheng, Un-Hwan Ha, Weihui Wu
Summary: This study reveals a novel regulatory mechanism in Pseudomonas aeruginosa, where CspC protein regulates the quorum-sensing systems by repressing the translation of a negative regulator gene, rsaL, in response to a host-generated metabolite.
Article
Biochemistry & Molecular Biology
Suqi Hao, Dan Yang, Ling Zhao, Fei Shi, Gang Ye, Hualin Fu, Juchun Lin, Hongrui Guo, Ran He, Jianlong Li, Hongwei Chen, Muhammad Faraz Khan, Yinglun Li, Huaqiao Tang
Summary: EGCG, a bioactive ingredient in green tea, demonstrated significant inhibitory effects on the production of virulence factors in Pseudomonas aeruginosa, potentially serving as a candidate for quorum sensing inhibition to combat bacterial infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Zhidong Zhang, Yang Sun, Yuanyang Yi, Xiaoyu Bai, Liying Zhu, Jing Zhu, Meiying Gu, Yanlei Zhu, Ling Jiang
Summary: A strain of Streptomyces with quorum-sensing inhibitory activity was isolated from the Kumutag Desert in Xinjiang, and it effectively inhibited biofilm formation and virulence factor production in bacteria such as Pseudomonas aeruginosa.
Article
Microbiology
Rakesh Sikdar, Mikael H. Elias
Summary: Quorum sensing (QS) is a cell-to-cell communication system that regulates bacterial behaviors. Enzymatic quorum quenching (QQ) can interfere with QS and potentially enhance the effects of antibiotics. This study evaluated the impact of QS signal disruption on antibiotic resistance in Pseudomonas aeruginosa and found that it significantly affects resistance. The results highlight the complex relationship between QS and antibiotic resistance and suggest a case-by-case approach to combination therapy.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Sahil Shandil, Tsz Tin Yu, Shekh Sabir, David StC. Black, Naresh Kumar
Summary: As bacteria develop resistance against antimicrobials, alternative methods to tackle this issue must be developed. In this study, quinazolinone inhibitors were designed using molecular docking to target the pqs system in P. aeruginosa. Compound 6b showed the highest pqs inhibitory activity and no bacterial growth inhibition.
Article
Microbiology
Pauline Nogaret, Fatima El Garah, Anne-Beatrice Blanc-Potard
Summary: A novel vertebrate animal model using zebrafish was developed to assess drug efficacy against P. aeruginosa, with validation using a clinically used antibiotic and a novel quorum sensing inhibitory molecule. This study provides a new and reliable method for comparing the virulence of P. aeruginosa strains in vivo and evaluating the efficacy of antipseudomonal drugs, including new antivirulence compounds.
Article
Biochemistry & Molecular Biology
Avi Neznansky, Inna Blus-Kadosh, Gal Yerushalmi, Ehud Banin, Yarden Opatowsky
Article
Cell & Tissue Engineering
Adi Har-Zahav, Daniela Lixandru, David Cheishvili, Ioan Valentin Matei, Ioana Raluca Florea, Veronica Madalina Aspritoiu, Inna Blus-Kadosh, Irit Meivar-Levy, Andreea Madalina Serban, Irinel Popescu, Moshe Szyf, Sarah Ferber, Simona Olimpia Dima
Summary: The study examines the relationship between DNA methylation and liver to pancreas transdifferentiation. The results show that the transdifferentiation process is associated with DNA demethylation and increased expression of specific genes.
STEM CELL RESEARCH & THERAPY
(2022)