Article
Food Science & Technology
Liuke Liang, Haojie Yang, Shan Wei, Shuaibing Zhang, Liang Chen, Yuansen Hu, Yangyong Lv
Summary: This study characterized the role of AflZKS3, a putative C2H2 transcription factor, in the growth and aflatoxin biosynthesis of A. flavus. The deletion of AflZKS3 resulted in defective growth, conidia formation, and increased sensitivity to stress. The biosynthesis of aflatoxin B-1 was completely inhibited in the deletion strain, and its ability to infect peanut and corn seeds was also reduced. These findings provide insights into the functions of C2H2 transcription factors in A. flavus and suggest AflZKS3 as a potential biocontrol target for preventing aflatoxin contamination.
Article
Cell Biology
Xiaoyu Li, Yanxia Zhao, Heungyun Moon, Jieyin Lim, Hee-Soo Park, Zhiqiang Liu, Jae-Hyuk Yu
Summary: VadH is a crucial transcription factor in Aspergillus nidulans, involved in regulating asexual/sexual development, osmotic stress response, and mycotoxin sterigmatocystin production.
Article
Microbiology
Sung-Hun Son, Mi-Kyung Lee, Ye-Eun Son, Hee-Soo Park
Summary: HbxB in Aspergillus nidulans functions as a key transcription factor regulating trehalose biosynthesis, stress tolerance, beta-glucan degradation, and sterigmatocystin production in conidia. Its deletion affects gene expression associated with these processes, leading to increased sensitivity to UV stress and alterations in beta-glucan levels and sterigmatocystin production.
Article
Biochemistry & Molecular Biology
Betim Karahoda, Lakhansing Pardeshi, Mevlut Ulas, Zhiqiang Dong, Niranjan Shirgaonkar, Shuhui Guo, Fang Wang, Kaeling Tan, Ozlem Sarikaya-Bayram, Ingo Bauer, Paul Dowling, Alastair B. Fleming, Brandon T. Pfannenstiel, Dianiris Luciano-Rosario, Harald Berger, Stefan Graessle, Mohamed M. Alhussain, Joseph Strauss, Nancy P. Keller, Koon Ho Wong, Ozgur Bayram
Summary: This study describes a chromatin binding complex KERS in the fungus Aspergillus nidulans, consisting of H3K4 histone demethylase KdmB, cohesin acetyltransferase EcoA, histone deacetylase RpdA, and histone reader/E3 ligase protein SntB. The KERS complex exerts promoter-specific transcriptional effects at active core promoters.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Ye-Eun Son, Jae-Hyuk Yu, Hee-Soo Park
Summary: This study identified and investigated the role of the spore-specific transcription factor SscA in three Aspergillus species. It was found that SscA plays a crucial role in the formation, maturation, germination, and integrity of conidia, as well as in amino acid production and secondary metabolism. Importantly, this role is conserved in other Aspergillus species.
Article
Biotechnology & Applied Microbiology
Akihiro Ninomiya, Syun-ichi Urayama, Daisuke Hagiwara
Summary: Co-culturing fungi with other microorganisms enhances the production of secondary metabolites. In this study, the activation of specific gene expressions in the co-culture system of two fungal species resulted in the production of different compounds compared to previous co-culture studies with bacteria. The molecular genetic mechanisms behind the biosynthesis of these compounds were demonstrated.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yong-Ho Choi, Sang-Cheol Jun, Min-Woo Lee, Jae-Hyuk Yu, Kwang-Soo Shin
Summary: The deletion of the mbsA gene in Aspergillus fumigatus results in impaired growth and sporulation, altered spore wall structure, increased gliotoxin production but reduced virulence, potentially due to defects in the SakA signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Yuzhen Li, Ziming Chen, Feng Zhang, Tianming Chen, Junxia Fan, Xin Deng, Xiaocui Lei, Bin Zeng, Zhe Zhang
Summary: This study reveals the role of the C2H2-type zinc-finger protein AoKap5 in the growth and kojic acid biosynthesis of A. oryzae. AoKap5 regulates kojic acid production through binding to the kojT promoter.
FUNGAL GENETICS AND BIOLOGY
(2023)
Article
Microbiology
Sanjoy Paul, Paul Bowyer, Michael Bromley, W. Scott Moye-Rowley
Summary: In this study, we characterized a gene designated ffmA as a sequence-specific transcriptional regulator involved in azole drug resistance and normal growth in Aspergillus fumigatus. Loss of ffmA caused sensitivity to azole drugs and a large reduction in normal growth. Overproduction of the AtrR transcription factor restored growth to ffmA null cells. Our data indicated that FfmA can recognize promoters of genes involved in azole resistance as well as its own promoter. This study sheds light on the mechanisms underlying azole resistance and normal growth in A. fumigatus.
Review
Plant Sciences
Huan Han, Caini Wang, Xiaoyan Yang, Lina Wang, Jiabao Ye, Feng Xu, Yongling Liao, Weiwei Zhang
Summary: This study provides an overview of the structure, classification, regulatory mechanisms, and biological functions of basic leucine zipper transcription factors (bZIPs) and their molecular mechanisms in the biosynthesis of various secondary metabolites. bZIPs are highly conserved transcription factors in eukaryotic organisms and play crucial roles in plant growth and development, stress response, and secondary metabolism. The paper elaborates on the structure, classification, biological function, and regulatory mechanisms of bZIPs, as well as their molecular mechanisms in regulating the biosynthesis of flavonoids, terpenoids, alkaloids, phenolic acids, and lignin. This review is significant for understanding the molecular mechanisms of bZIPs in secondary metabolite synthesis and plant molecular breeding.
Article
Microbiology
Yong Chen, Zhanquan Zhang, Boqiang Li, Shiping Tian
Summary: The study highlights the crucial role of the PeMetR gene in sulfur metabolism, virulence, and patulin biosynthesis in Penicillium expansum. PeMetR is essential for sulfur assimilation and its disruption results in loss of virulence and reduced patulin production. Regulation of sulfur assimilation by PeMetR affects growth, infection, and biosynthesis of the mycotoxin patulin in P. expansum.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Hongchen Wang, Renwei Gao, Yuanwei Zhang, Ling Lu
Summary: Transient receptor potential (TRP) channels play crucial roles in sensory signals and cellular functions. This study investigated the biological functions of three potential TRP channels (TrpA, TrpB, and TrpC) in Aspergillus nidulans. The findings revealed that these TRP channels have distinct localization patterns and may be involved in different physiological processes. Deletion or reduced expression of TrpA resulted in severe growth defects and abolished conidiation. The lack of TrpB led to colony defects in a calcium-dependent manner, while the deletion of TrpC did not show detectable phenotypes. These findings provide valuable insights into the functions of TRP proteins in fungi.
Article
Microbiology
Patricia Sequeira, Maika Rothkegel, Patricia Domingos, Isabel Martins, Celine C. Leclercq, Jenny Renaut, Gustavo H. Goldman, Cristina Silva Pereira
Summary: Fungal secondary metabolites are a rich source of undiscovered bioactive compounds. Altering cultivation conditions can trigger the production of certain compounds. Using ionic liquids as growth medium supplements can greatly impact the biosynthesis of secondary metabolites, leading to increased diversity of extracellular compounds. This study investigated the ability of cholinium-based ionic liquid supplements to support the discovery of bioactive secondary metabolites in three model species, and found potential antibacterial activity in the enriched organic extracts.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Aleksandr A. Arzamasov, Aruto Nakajima, Mikiyasu Sakanaka, Miriam N. Ojima, Takane Katayama, Dmitry A. Rodionov, Andrei L. Osterman
Summary: This study describes the NagR-mediated transcriptional regulation of WI and LNnT utilization in Bifidobacterium longum subsp. infantis. The elucidated regulatory network appears optimally adapted to simultaneous utilization of multiple HMOs, providing a rationale to add HMO mixtures (rather than individual components) to infant formulas. The study also provides insights into the evolutionary trajectories of complex regulatory networks controlling carbohydrate metabolism in bifidobacteria.
Article
Microbiology
Mario K. C. Krespach, Maria C. Stroe, Tina Netzker, Maira Rosin, Lukas M. Zehner, Anna J. Komor, Johanna M. Beilmann, Thomas Krueger, Kirstin Scherlach, Olaf Kniemeyer, Volker Schroeckh, Christian Hertweck, Axel A. Brakhage
Summary: The soil bacterium Streptomyces iranensis triggers the production of natural products in the fungus Aspergillus nidulans using arginine-derived polyketide signalling molecules. These molecules, called arginoketides, can be cyclic or linear and induce the production of natural products in fungi of the genera Aspergillus and Penicillium. The presence of arginoketide producers and fungi that respond to this signal in the same soil sample suggests a widespread occurrence of such microbial interactions.
NATURE MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jessica M. Lohmar, Olivier Puel, Jeffrey W. Cary, Ana M. Calvo
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Genetics & Heredity
Jeffrey W. Cary, Sarah Entwistle, Timothy Satterlee, Brian M. Mack, Matthew K. Gilbert, Perng K. Chang, Leslie Scharfenstein, Yanbin Yin, Ana M. Calvo
G3-GENES GENOMES GENETICS
(2019)
Article
Genetics & Heredity
Sandesh S. Pandit, Jessica M. Lohmar, Shawana Ahmed, Oier Etxebeste, Eduardo A. Espeso, Ana M. Calvo
Article
Multidisciplinary Sciences
Binita Nepal, Ryan Myers, Jessica M. Lohmar, Olivier Puel, Brett Thompson, Matthew Van Cura, Ana M. Calvo
Article
Biotechnology & Applied Microbiology
Tim Satterlee, Binita Nepal, Sophie Lorber, Olivier Puel, Ana M. Calvo
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Genetics & Heredity
Timothy Satterlee, Sarah Entwistle, Yanbin Yin, Jeffery W. Cary, Matthew Lebar, Liliana Losada, Ana M. Calvo
G3-GENES GENOMES GENETICS
(2019)
Article
Genetics & Heredity
Thaila Fernanda dos Reis, Laura Mellado, Jessica M. Lohmar, Lilian Pereira Silva, Jing-Jiang Zhou, Ana M. Calvo, Gustavo H. Goldman, Neil A. Brown
Article
Microbiology
Daniel Peterson, Tang Li, Ana M. Calvo, Yanbin Yin
Summary: The study provides new insights into phytopathogenicity in Ascomycota through comparative genomics, revealing that group-specific orthogroups have more enriched functional terms and certain functional terms show higher significance and occurrence in these orthogroups. The analysis also suggests that genes shared by multiple phytopathogenic genomes are more important for phytopathogenicity than unique genes in each genome.
Article
Chemistry, Analytical
R. Jayalakshmi, S. T. Mehetre, R. Kannan, M. Paramasivam, V. P. Santhanakrishnan, K. K. Kumar, M. Theradimani, V Ramamoorthy
Summary: This study assessed the efficiency of various solvents in extracting gliotoxin from soil and crop plants, and found that chloroform and ethyl acetate were effective solvents for extraction.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
R. Jayalakshmi, R. Oviya, K. Premalatha, S. T. Mehetre, M. Paramasivam, R. Kannan, M. Theradimani, M. S. Pallavi, Prasun K. Mukherjee, V Ramamoorthy
Summary: Gliotoxin production by Trichoderma virens is influenced by the growth medium pH, with stability in acidic water but degradation in alkaline water. Soil moisture, microbial community, and pH conditions play a major role in the degradation of gliotoxin in soil ecosystems.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
P. Suresh, V. Shanmugaiah, Rajakrishnan Rajagopal, K. Muthusamy, V. Ramamoorthy
Summary: Plant growth-promoting rhizobacteria (PGPR) are potential bio agents for suppressing soil-borne diseases in crop plants. This study demonstrated that Pseudomonas fluorescens can induce defense enzymes and genes in tomato plants, effectively suppressing wilt disease caused by Ralstonia solanacearum.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2022)
Article
Microbiology
Perumal Suresh, Murukesan Rekha, Subramanian Gomathinayagam, Vellaisamy Ramamoorthy, Mahaveer P. Sharma, Perumal Sakthivel, Karuppannan Sekar, Mariadhas Valan Arasu, Vellasamy Shanmugaiah
Summary: Microbial bio-products, such as 2,4-diacetylphloroglucinol (DAPG) produced by Pseudomonas fluorescens, have potential as an alternative to chemical pesticides for managing crop diseases. In this study, the inhibitory efficacy of DAPG against Ralstonia solanacearum was assessed. The study also revealed the beneficial characteristics of Pseudomonas fluorescens, such as the production of antimicrobial substances and the ability to solubilize phosphate. Additionally, the application of Pseudomonas fluorescens and DAPG reduced bacterial wilt incidence and promoted the growth of tomato seedlings.
Article
Literature
Paula Barba Guerrero, Ana Ma Manzanas Calvo
CLCWEB-COMPARATIVE LITERATURE AND CULTURE
(2019)
Article
Biotechnology & Applied Microbiology
Beatriz A. Ibarra, Jessica M. Lohmar, Timothy Satterlee, Taylor McDonald, Jeffrey W. Cary, Ana M. Calvo
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Literature, African, Australian, Canadian
Ana Maria Manzanas-Calvo
CANADIAN LITERATURE
(2017)