Article
Anatomy & Morphology
Jia-Ming Wang, Zhen-Fang Li, Hong-Yu Qi, Zhan Zhao, Wan-Xi Yang
Summary: The actin binding proteins es-Arp3 and es-Eps8 are found to be crucial for cell adhesion and spermatogenesis in the Chinese mitten crab. Their deficiency results in delayed spermatozoa release, shedding of germ cells, disorganized actin filaments, and abnormal expression of junctional proteins.
Article
Biochemistry & Molecular Biology
Ding -Xi Liu, Zhen-Fang Li, Yan-Shuang Zhao, Lan-Min Wang, Hong -Yu Qi, Zhan Zhao, Fu-Qing Tan, Wan-Xi Yang
Summary: The study found that Es-beta-CATENIN participates in maintaining the hemolymph-testes barrier in the spermatogenesis of E. sinensis. By interacting with other proteins, it maintains the integrity of the hemolymph-testes barrier and also regulates cell proliferation and apoptosis, playing a crucial role in normal sperm release.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Marine & Freshwater Biology
Rui Jia, Xiaoling Dai, Yanfang Li, Xintong Yang, Xiuwen Min, Derun Quan, Peng Liu, Xin Huang, Jiachun Ge, Qian Ren
Summary: This study found that nitrite stress could increase the likelihood of Eriocheir sinensis being infected by White Spot Syndrome Virus (WSSV), and activate Duox to produce reactive oxygen species (ROS), which inhibited the synthesis of WSSV protein VP28. Furthermore, nitrite stress could promote the expression of EsDorsal transcription factor and antimicrobial peptides (AMPs), and AMP synthesis was positively regulated by EsDorsal during WSSV infection.
AQUATIC TOXICOLOGY
(2023)
Article
Fisheries
Binghui Yang, Jinlong Ma, Wen Yang, Chen Qu, Bing Li, Mei Xu, Yujia Gao, Qingsong Xu
Summary: The study investigated the role of an MEK homologue gene (EsMEK) in the Chinese mitten crab Eriocheir sinensis. EsMEK was found to be constitutively expressed in all tissues, with higher expression in hepatopancreas, hemocytes, and gills. LPS and Aeromonas hydrophila challenge increased EsMEK mRNA levels in hemocytes, and inhibition of EsMEK led to decreased expression of antimicrobial peptides.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Biology
Ke Jia, Fu-Qing Tan, Shuang-Li Hao, Wan-Xi Yang
Summary: This study revealed the crucial role of Es-SOX8 protein in the spermatogenesis of E. sinensis, and showed that it can directly regulate the transcription of Es-BMP2, thus affecting the sperm nucleus deformation process.
Article
Fisheries
Ying Huang, Qin Si, Shenghao Du, Jie Du, Qian Ren
Summary: In this study, a new member of the TNF superfamily, EsTNFSF, was identified from the Chinese mitten crab. EsTNFSF was found to play a crucial role in the antibacterial immune defense of E. sinensis.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Xiaona Zhang, Guoqing Shen, Yanan Guo, Xiaoli Zhang, Yuehong Zhao, Weiwei Li, Qun Wang, Yunlong Zhao
Summary: Protein kinases of the MAPK cascade family play a crucial role in the growth and response to stress in organisms. This study identified and analyzed four MAPKK genes in the Chinese mitten crab Eriocheir sinensis. The genes were found to have conserved domains and motifs and were expressed in various tissues, with higher expression in hemocytes, hepatopancreas, and gills. Infection with Vibrio parahaemolyticus increased the mRNA levels of the MAPKK genes in hemocytes, and disruption of their expression affected the levels of antimicrobial peptides. These findings provide insights into the innate immunity of E. sinensis.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yong Lei, Yanfang Sun, Xiaodan Wang, Zhideng Lin, Xianyong Bu, Nan Wang, Zhenyu Du, Jianguang Qin, Liqiao Chen
Summary: The study demonstrated that optimal dietary phosphorus levels can significantly affect the growth, antioxidant capacity, and lipid metabolism of juvenile Chinese mitten crab. A recommended available phosphorus range in the diet was suggested to be 1.16% to 1.51%.
Article
Biology
Shuang-li Hao, Fei-da Ni, Bang-hong Wei, Zhen-fang Li, Tong Yang, Wan-xi Yang
Summary: The study found that es-ATG7 plays a crucial role in maintaining sperm morphology and reducing cell apoptosis during spermatogenesis in the Chinese mitten crab. Additionally, es-ATG7 acts as an antagonist for p53-dependent apoptosis induction in this process.
Article
Agricultural Engineering
Yuan-Yuan Ji, Jun-Yi Yang, Rui-Fei Zhang, Qing-Yu Chen, Rui Xu, Xue-Jin Wei, Xiao-Hang Chen, Shan-Xi Chen, Fan-Ding Guo, Edward J. Kennelly, Chun-Lin Long
Summary: St. John's wort and other Hypericum species are used as traditional medicine in southwestern China for treating depression, wounds, and skin problems. The volatile oils of these plants contain various compounds with activities such as neurite outgrowth-promoting, neuroprotective, antimicrobial, and enzyme inhibitory effects. The oils from different species showed significant differences in constituents and activities.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Bui Thi Mai Anh, Do Thi Trang, Hoang Thi Tuyet Lan, Phan Van Kiem, Bui Huu Tai, Nguyen Viet Dung, Ninh Khach Thanh Nam, Nguyen The Cuong, Nguyen Xuan Nhiem, Nguyen Thi Mai
Summary: One new phenylpropanoid glycoside and 13 known compounds were isolated from Tinospora sinensis (Lour.) Merr. The phenylpropanoid glycoside showed significant inhibitory activity against NO production in macrophages. Several other compounds also exhibited moderate NO inhibitory activity.
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
(2023)
Article
Microbiology
Cho Rong Park, Seungwha Paik, Young Jae Kim, Jin Kyung Kim, Sang Min Jeon, Sang-Hee Lee, Jake Whang, Jinhua Cheng, Joo-Won Suh, Jin Cao, Gauri Shetye, Shao-Nong Chen, James McAlpine, Guido F. Pauli, Scott Franzblau, Sanghyun Cho, Eun-Kyeong Jo
Summary: This study demonstrates that Rufomycin 4-7 exhibits dual effects against Mycobacterium abscessus, showing both antimicrobial activities and enhancing host immune defense. It effectively inhibits inflammation, promotes autophagy/lysosomal genes, and prevents mitochondrial damage and oxidative stress in macrophages during Mabs-R infection, potentially providing an advantage in treating Mabs-R infection.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Chemistry, Medicinal
Ashraf A. Abbas, Magda H. Abdellattif, Kamal M. Dawood
Summary: This review discusses the biological and pharmacological potentials of bipyrazole derivatives from 2010 to 2021 including anticancer, antioxidant, anti-inflammatory, antimicrobial, antitubercular, antimalarial, insecticidal activities, and enzymatic inhibitions. Bipyrazoles show promising activities against various diseases, making them valuable for medicinal researchers. Metallated bipyrazoles, such as gold(III) and iridium(II) complexes, exhibit better biological activities than bipyrazole ligands, while copper(I) and silver(I) complexes show excellent antibacterial activities. Several bipyrazoles are reported to be better antimalarial inhibitors than chloroquine, a potential drug for COVID-19.
EXPERT OPINION ON THERAPEUTIC PATENTS
(2022)
Review
Chemistry, Medicinal
Thoraya A. Farghaly, Kamal M. Dawood
Summary: This mini-review provides a comprehensive evaluation of pyrazolo-oxazine derivatives, offering a pathway for further exploration in medicinal and pharmacological research.
MINI-REVIEWS IN MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Ying Huang, Xi-Mei Nie, Zheng-Jie Zhu, Xing Zhang, Bing-Zhi Li, Jia-Chun Ge, Qian Ren
Summary: A novel c-Jun NH2-terminal kinase (EsJNK) was identified and characterized in the Chinese mitten crab Eriocheir sinensis, with a determined cDNA sequence and predicted tertiary structure. EsJNK was found to be closely related to JNK homologs in other crustaceans and exhibited relatively higher expression in hemocytes, muscles, and intestines. Functional studies indicated that EsJNK may play a role in regulating the transcription of antimicrobial peptides and be involved in the antibacterial immune defense of crabs.
MOLECULAR IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xingkun Jin, Ziwei Chen, Yan Shi, Jian-Fang Gui, Zhe Zhao
Summary: Environmental bacteria have a significant impact on fish gut microbiota, with feed-associated bacteria causing disturbance to the hindgut microbiota of cultured fugu. Community stability is largely influenced by homogeneous selection and dispersal limitation under low disturbance, while increased disturbance leads to variable selection and enhanced interaction within the gut microbiota, resulting in instability and shifts in the community composition.
MICROBIAL BIOTECHNOLOGY
(2022)
Review
Fisheries
Weiwei Li, Qun Wang
Summary: Crustaceans, such as crabs and shrimp, lack adaptive immunity and rely solely on innate immunity for pathogen defense. The white spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimps and is a widely prevalent viral disease. Crabs serve as interesting models for studying immune responses to WSSV infection, with recent reviews focusing on antiviral functions such as exosome-mediated apoptosis and alternatively spliced Down syndrome cell adhesion molecule.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Yu Song, Kaimin Zhou, Xingyu Nan, Yukai Qin, Ke Zhao, Weiwei Li, Qun Wang
Summary: The study found that ML proteins were involved in bacterial resistance in Chinese mitten crab, with one member, EsML3, being upregulated in hemocytes following bacterial challenge and binding to Gram-negative and Gram-positive bacteria. EsML3 mediates cellular immune responses by promoting bacterial clearance and phagocytosis against bacterial infection in crab.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Environmental Sciences
Xingkun Jin, Yaofang Yang, Haihang Cao, Beile Gao, Zhe Zhao
Summary: Cobalamin, an essential micronutrient, is crucial for both prokaryotes and eukaryotes. Some Cyanobacterial and Archaeal taxa can synthesize cobalamin de novo, but the contributions of Gammaproteobacteria like the 'Psychromonadaceae' remain unknown. Uneven distribution of cobalamin biosynthetic operon and cobalamin-dependent light-responsive regulon was observed, suggesting ecological divergence impacts their metabolic pathways.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Fisheries
Hao Li, Yuehong Zhao, Xiaoli Zhang, Hui Zhao, Weiwei Li, Qun Wang
Summary: In this study, the transcriptional response of Eriocheir sinensis to different forms of Dscam ICDs was investigated. The results showed that different forms of Dscam ICDs can affect key immune, metabolic, and cell proliferation-regulated genes and pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Kaimin Zhou, Longwei Bai, Xingyu Nan, Ke Zhao, Yu Song, Weiwei Li, Qun Wang
Summary: In this study, the essential role of FADD in the IMD signaling pathway in the Chinese mitten crab Eriocheir sinensis was demonstrated. Knockdown of EsFADD led to impaired immune response and increased susceptibility to bacterial infection. The findings highlight the evolutionary conservation of FADD in crustaceans and provide insights into innate immune defense in invertebrates.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Jiying Li, Ke Zhao, Hao Li, Kaimin Zhou, Qun Wang, Weiwei Li
Summary: The study revealed that the Dscam gene in the Chinese mitten crab has different variants that play a role in immune regulation, with significant increase in expression levels after bacterial stimulation. The two variants also differ in their regulation of antimicrobial peptides, proportion of carried exons, and bacterial phagocytosis.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hui Zhao, Zhe Chen, Hao Li, Yue-Hong Zhao, Qun Wang, Wei-Wei Li
Summary: This study demonstrates that bacteria can induce hemocyte proliferation in Chinese mitten crabs, and EsCsn5 plays a negative regulatory role in this process by controlling the degradation of EsCyclin E. Knockdown of EsCsn5 enhances hemocyte proliferation and reduces survival rate in crabs, leading to increased bacterial concentration in the hemolymph.
Article
Immunology
Kaimin Zhou, Yukai Qin, Yu Song, Ke Zhao, Weijuan Pan, Xingyu Nan, Yue Wang, Qun Wang, Weiwei Li
Summary: The study reveals the antibacterial role of a member of the C-type lectin family, EsIgLectin, in the Chinese mitten crab. EsIgLectin is expressed in hemocytes and intestines, and it plays a critical role in bacterial binding, agglutination, growth inhibition, and clearance. It promotes bacterial phagocytosis in hemocytes and protects against inflammation in the intestines, functioning in a polymeric Ig receptor-dependent manner.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Microbiology
Xixi Li, Ce Zhang, Xingkun Jin, Fucheng Wei, Fei Yu, Douglas R. Call, Zhe Zhao
Summary: Phage HH109 was found to exhibit control over host genes during infection, inducing rapid and distinctive changes. Gene co-expression analysis predicted that the late gene gp27 encoded by HH109 modulates the host's membrane transport and/or transcriptional regulation. Additionally, bacterial virulence genes were downregulated while drug resistance genes were upregulated.
Article
Fisheries
Yue Wang, Xingyu Nan, Shuqi Hao, Ke Zhao, Yanan Guo, Qun Wang, Weiwei Li
Summary: AKT plays an important role in regulating cell metabolism and cell proliferation in Eriocheir sinensis. The EsAkt protein is highly conserved across species, and its expression is up-regulated in hemocytes after stimulation with gram-positive or gram-negative bacteria. Knockout of the EsAkt gene reduces cell glucose metabolism and inhibits cell proliferation.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Editorial Material
Physiology
Xingkun Jin, Xiaodan Wang, William Ka Fai Tse, Yan Shi
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Fisheries
Zi-Wei Chen, Xing-Kun Jin, Fan-Xiang Gao, Jian-Fang Gui, Zhe Zhao, Yan Shi
Summary: The structure and function of gut microbiota in fish can be influenced by fish sex, but the impact is limited, mainly affecting certain bacterial taxa. The relative abundance of sex-specific bacterial taxa plays a role in determining bacterial phenotypes.
Article
Environmental Sciences
Xingyu Nan, Xingkun Jin, Yu Song, Kaimin Zhou, Yukai Qin, Qun Wang, Weiwei Li
Summary: Using Chinese mitten crabs as a model, this study investigated the effects of polystyrene nanoplastics (PS NPs) on cell apoptosis, glucose metabolism, and antibacterial immunity. The results showed that PS NPs were endocytosed by crab hemocytes, leading to abnormal cell apoptosis, glucose metabolism disorder, and changes in antimicrobial immunity.
ENVIRONMENTAL POLLUTION
(2022)
Article
Microbiology
Xingkun Jin, Haoyong Zhu, Yan Shi, Ziwei Chen, Yaohui Wang, Jian-Fang Gui, Zhe Zhao
Summary: Through gut microbiota profiling, we found that hybridization and cohabitation of pufferfish can significantly disturb the gut microbiota composition and function. These results enhance our understanding of the complex and dynamic assemblage of the fish holobiont from a microbial perspective and have practical implications for optimizing performance prediction and improving farmed fish production.