Article
Infectious Diseases
Toyotaka Sato, Shin-ichi Yokota, Tooru Tachibana, Satoshi Tamai, Shigeki Maetani, Yutaka Tamura, Motohiro Horiuchi
Summary: The study found that companion animals can carry fluoroquinolone-resistant Escherichia coli, which may be associated with human clinical infections and can potentially be transmitted to their owners.
Article
Infectious Diseases
Zoi Athanasakopoulou, Martin Reinicke, Celia Diezel, Marina Sofia, Dimitris C. Chatzopoulos, Sascha D. Braun, Annett Reissig, Vassiliki Spyrou, Stefan Monecke, Ralf Ehricht, Katerina Tsilipounidaki, Alexios Giannakopoulos, Efthymia Petinaki, Charalambos Billinis
Summary: This study investigated the multidrug resistance phenotype and genotype of ESBL-producing Escherichia coli isolates from livestock and wild bird sources in Greece. The results showed that these isolates carried various antibiotic resistance genes, indicating their dissemination in diverse ecosystems and highlighting the importance of a One-Health approach in addressing antimicrobial resistance.
Article
Microbiology
Gaelle Gruel, David Couvin, Stephanie Guyomard-Rabenirina, Guillaume Arlet, Jean-Christophe Bambou, Matthieu Pot, Xavier Roy, Antoine Talarmin, Benoit Tressieres, Severine Ferdinand, Sebastien Breurec
Summary: This study determines the prevalence of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) in feces of household and shelter pets in Guadeloupe. The study finds that the only risk factor associated with a higher prevalence of ESBL-E rectal carriage is a stay in a shelter. The study also identifies ESBL-E as mainly Escherichia coli, with certain plasmids carrying antimicrobial resistance genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Antonio Mandujano, Diana Veronica Cortes-Espinosa, Jose Vasquez-Villanueva, Paulina Guel, Gildardo Rivera, Karina Juarez-Rendon, Wendy Lizeth Cruz-Pulido, Guadalupe Aguilera-Arreola, Abraham Guerrero, Virgilio Bocanegra-Garcia, Ana Veronica Martinez-Vazquez
Summary: This study investigated the antimicrobial resistance profile of ESBL-producing E. coli among food-producing animals in Tamaulipas, Mexico. A total of 200 fecal samples were collected and 5.0% of the strains were confirmed as ESBL producers. High percentage of antimicrobial resistance was observed against gentamicin, tetracycline, and streptomycin. The study suggests that food-producing animals may serve as reservoirs and contribute to the spread of ESBL-producing bacteria.
Article
Infectious Diseases
Delveen R. Ibrahim, Christine E. R. Dodd, Dov J. Stekel, Remilekun T. Meshioye, Mathew Diggle, Michelle Lister, Jon L. Hobman
Summary: Cephalosporin therapy failure is a serious problem worldwide for infection control. Cephalosporin-resistant infections are often caused by multidrug-resistant E. coli producing ESBLs and/or pAmpC beta-lactamases. Analysis of MDR E. coli isolates from a UK hospital revealed a high prevalence of ESBL/pAmpC genetic determinants and significant correlations between resistance genes and specific antibiotics. The findings emphasize the importance of improved antibiotic therapy guidelines, including reduced usage and enhanced surveillance.
Article
Biotechnology & Applied Microbiology
Muhammad Shafiq, Sadeeq Ur Rahman, Hazrat Bilal, Aftab Ullah, Sohail Muhammad Noman, Mi Zeng, Yumeng Yuan, Qingdong Xie, Xin Li, Xiaoyang Jiao
Summary: This study investigated the occurrence and molecular features of ESBL-producing and colistin-resistant E. coli isolates from healthy food-producing animals in Pakistan. The results showed a high proportion of ESBL and colistin resistance in the fecal samples, with most isolates exhibiting multidrug resistance. Various resistant determinants and mobile genetic elements were also found. These findings pose a significant threat to both animals and human communities.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Infectious Diseases
Adriana Belas, Catia Marques, Juliana Menezes, Luis Telo da Gama, Patricia Cavaco-Silva, Constanca Pomba
Summary: This study aimed to compare ESBL/pAmpC-producing E. coli strains causing community-acquired UTI in companion animals and humans. The study found that companion animals and humans shared two multidrug-resistant high-risk clonal lineages: ST131 and ST648. The study also reported two newly discovered clonal lineages in humans with community-acquired UTI in Portugal.
Article
Infectious Diseases
Weishuai Zhai, Tao Wang, Dawei Yang, Qidi Zhang, Xiao Liang, Zhihai Liu, Chengtao Sun, Congming Wu, Dejun Liu, Yang Wang
Summary: This study investigated the relationship of tet(X4)-positive isolates from different hosts and environments. The results showed that these isolates exhibited multidrug resistance, and the majority of them belonged to separate clonal groups. Among these isolates, the E. coli ST761 strains were widely distributed in animals, food, wastewater, and humans. There was a close phylogenetic relationship among these strains, suggesting a potential transmission of tet(X4)-positive E. coli ST761 between humans and animals. Additionally, this study also reported the first description of tet(X4)-positive A. simiae.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Mayo Yasugi, Shingo Hatoya, Daisuke Motooka, Daisuke Kondo, Hideo Akiyoshi, Masayuki Horie, Shota Nakamura, Terumasa Shimada
Summary: The presence of mcr plasmid-mediated colistin-resistant ESBL-producing Enterobacterales in companion dogs and cats in Japan was identified. The isolates from a dog and a cat carried different mcr genes and bla genes on plasmids. This suggests that companion dogs and cats may serve as important reservoirs for cross-species transmission of the mcr gene.
VETERINARY MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
M. Shafiq, J. Huang, J. M. Shah, X. Wang, S. U. Rahman, I. Ali, L. Chen, L. Wang
Summary: The study found a high level of multi-drug resistance in ESBL and colistin-resistant E. coli isolates from bovine mastitis, with dominance of the bla(CTX-M-1) group. Targeted virulence genes were detected in the majority of isolates, indicating a high diversity of E. coli strains. The association between ESBL-producing E. coli and mcr-1 raised particular concern.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Infectious Diseases
Michael Kresken, Esther Wohlfarth, Chase Weikel, Deborah Butler, Yvonne Pfeifer, Guido Werner, Paul Ehrlich Soc Infection Therapy
Summary: Gepotidacin demonstrated promising in vitro activity against urine isolates of E. coli, including ciprofloxacin-resistant isolates, ESBL-producing isolates, and isolates resistant to oral standard-of-care antibiotics.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Sandra Martinez-Alvarez, Susana Sanz, Carmen Olarte, Raquel Hidalgo-Sanz, Isabel Carvalho, Rosa Fernandez-Fernandez, Allelen Campana-Burguet, Javier Latorre-Fernandez, Myriam Zarazaga, Carmen Torres
Summary: This study reveals the presence and extensive dissemination of antimicrobial-resistant E. coli, particularly ESBL-producing SHV-12 type, in the broiler farm environment.
Article
Microbiology
Hanseob Shin, Yongjin Kim, Tatsuya Unno, Hur Hor-Gil
Summary: In this study, the CRISPR loci in E. coli isolates from various sources were characterized. Most of the CRISPR spacers were found to be derived from phages. These spacers can help trace the infection history of bacteria and have potential applications in microbial source tracking.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Kathleen Klaper, Jens Andre Hammerl, Jorg Rau, Yvonne Pfeifer, Guido Werner
Summary: The study investigated 94 Klebsiella spp. isolates from different animal and food sources in Germany, revealing genetic diversity. ESBL genes and iron carrier (iuc) genes were detected in the isolates. The siderophore aerobactin (iuc lineage 3) was found to be more prevalent in K. pneumoniae strains isolated from pigs.
Article
Infectious Diseases
Yijun Ding, Jinjing Zhang, Kaihu Yao, Wei Gao, Yajuan Wang
Summary: This study assessed the antimicrobial resistance and molecular characteristics of E. coli ST1193 isolates causing neonatal bloodstream infections and meningitis in China, revealing that ST1193 isolates were often multidrug-resistant. Further multicenter studies are needed to control the spread of this emerging fluoroquinolone-resistant clone.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Oncology
Jose Javier Salgado Plonski, Sergio Fernandez-Pello, Laura Ruger Jimenez, Ivan Gonzalez Rodriguez, Laura Alonso Calvar, Luis Rodriguez Villamil
Summary: This study found that a higher body mass index (BMI > 25 kg/m(2)) significantly extends the overall survival of patients with metastatic renal cell carcinoma (RCC) undergoing systemic therapy. The results suggest that obesity may act as a protective factor in the treatment of metastatic RCC.
JOURNAL OF KIDNEY CANCER AND VHL
(2021)
Article
Primary Health Care
S. Cabrera Fernandez, M. D. Martin Martinez, C. De Francisco Montero, I Gabaldon Rodriguez, A. Vilches Arenas, M. Ortega Calvo
Summary: This study analyzed the diagnostic performance of different criteria in gestational diabetes and found significant differences in prevalence proportions based on the criteria applied. The study suggests that the GEDE criteria may be more efficient in diagnosing gestational diabetes compared to other criteria.
MEDICINA DE FAMILIA-SEMERGEN
(2021)
Article
Virology
Veronica Odinya Ameh, Guanghui Wu, Hooman Goharriz, Rebecca Shipley, Anthony R. Fooks, Claude T. Sabeta, Lorraine M. McElhinney
Summary: The study found that straw-colored fruit bats in Makurdi, Nigeria are commonly exposed to Lagos bat lyssavirus (LBV), with LBV-A likely circulating in the region. The majority of bat sera neutralized multiple LBV lineages, with reciprocal titers ranging from 9 to >420.89.
Article
Genetics & Heredity
Emma R. Holden, Muhammad Yasir, A. Keith Turner, John Wain, Ian G. Charles, Mark A. Webber
Summary: Biofilms undergo a life cycle involving gene expression regulation at different stages. Adhesion and motility are crucial in early biofilm formation, while matrix production and purine biosynthesis become more important as the biofilm matures. Temporal gene expression plays a significant role in biofilm fitness, with some genes showing stage-dependent effects. This study identifies novel genes involved in biofilm formation, expanding our understanding of the requirements for successful biofilm development through time.
MICROBIAL GENOMICS
(2021)
Article
Evolutionary Biology
Emma V. Waters, Liam A. Tucker, Jana K. Ahmed, John Wain, Gemma C. Langridge
Summary: Bacterial genome structure rearrangements around long repeat sequences can cause changes in gene expression. Multiplexed long-read sequencing enables the identification of genome structure rearrangements, which may provide a mechanism for bacteria to quickly adapt to new environments.
Article
Pharmacology & Pharmacy
Xinyu Wang, Lisanne Terrie, Guanghui Wu, Els J. M. Van Damme, Lieven Thorrez, Anthony R. Fooks, Ashley C. Banyard, Dirk Jochmans, Johan Neyts
Summary: Infection with the rabies virus is a deadly neurological disease, but post-exposure prophylaxis (PEP) with vaccination and anti-rabies immunoglobulins (RIGs) is 100% effective if administered early. This study found that Urtica dioica agglutinin (UDA), a specific lectin, can prevent the entry of the virus into host cells and effectively stop virus replication in a muscle model. UDA holds promise as a cheap and simple alternative for RIGs in PEP.
Article
Biochemical Research Methods
Guanghui Wu, Lorraine M. McElhinney, Hooman Goharriz, Joan Amaya-Cuesta, Anthony R. Fooks, Ashley C. Banyard
Summary: Rabies virus can be prevented through timely vaccination, and the levels of virus neutralising antibodies can be measured using the FAVN test. A fluorescent recombinant rabies virus, mCCCG, was constructed for simpler testing by inserting the gene for mCherry protein and replacing its glycoprotein. The use of mCCCG reduces assay time and eliminates the need for expensive antibody conjugates, making it beneficial for resource limited settings.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Urology & Nephrology
Pablo Sanchez Verdes, Sergio Fernandez-Pello Montes, Ivan Gonzalez Rodriguez, Pelayo Jose Suarez Sal, Lidia Martin Gomez, Luis Rodriguez Villamil
Summary: One-stop clinics have been established as a means to optimize cancer patient management. The objective of this study was to evaluate the impact of a one-stop hematuria clinic (OSHC) on the overall and disease-free survival of bladder cancer patients compared to a conventional clinic (CC). The results showed that OSHC significantly reduced the time to diagnosis and treatment, and decreased the early relapse rate, although the five-year survival rate was similar to CC.
ARCHIVOS ESPANOLES DE UROLOGIA
(2023)
Review
Medicine, General & Internal
Ileana Garcia Rodriguez, Mery Rosa Betancourt Trujillo, Lino Aristides Sanchez Galvan
Summary: Cutis verticis gyrata is a rare scalp pathology characterized by the proliferation of subcutaneous cellular tissue and hypertrophy that produces folds and furrows. Its etiology is still unknown, but a theory proposes the presence of autosomal dominant mutations in the fibroblast growth factor receptor 2. This pathology is extremely rare in pediatrics.
Article
Medicine, General & Internal
Juan Carlos Yanes Macias, Mercedes Fonseca Hernandez, Ileana Garcia Rodriguez, Caridad Llul Tombo, Daimy Tio Gonzalez, Juan Camilo Diaz Ceballos
Summary: Acute bronchiolitis requires significant care both in primary care and at the hospital level, with high assistance requirements in the emergency area and a significant number of admissions during epidemics, resulting in great economic costs. This article presents general considerations by consensus, focusing on the clinical and therapeutic aspects of the disease, to facilitate its management in primary care and hospitals.
MEDISUR-REVISTA DE CIENCIAS MEDICAS DE CIENFUEGOS
(2022)
Meeting Abstract
Psychiatry
C. Vilella Martin, P. Garcia Vazquez, I. Gonzalez Rodriguez, S. Nunez Sevillano, A. Serrano Garcia
EUROPEAN PSYCHIATRY
(2021)
Meeting Abstract
Psychiatry
C. Vilella Martin, M. A. Heredero Sanz, P. Garcia Vazquez, I. Gonzalez Rodriguez, S. Nunez Sevillano, A. Serrano Garcia
EUROPEAN PSYCHIATRY
(2021)
Meeting Abstract
Psychiatry
C. Vilella Martin, P. Garcia Vazquez, I. Gonzalez Rodriguez, S. Nunez Sevillano, A. Serrano Garcia
EUROPEAN PSYCHIATRY
(2021)
Meeting Abstract
Psychiatry
C. Vilella Martin, M. A. Alonso De la Torre Lopez, P. Garcia Vazquez, I. Gonzalez Rodriguez, S. Nunez Sevillano, A. Serrano Garcia
EUROPEAN PSYCHIATRY
(2021)
Article
Urology & Nephrology
Pablo Sanchez Verdes, Sergio Fernandez-Pello, Ivan Gonzalez Rodriguez, Jose Javier Salgado Plonski, Laura Alonso Calvar, Pelayo Jose Suarez Sal, Luis Rodriguez Villamil
Summary: A total of 1122 patients with gross hematuria were retrospectively studied, with 31.5% diagnosed with bladder tumors. The sensitivity and specificity of urinary cytology for urothelial tumor detection were 55.1% and 85.7%, respectively, indicating limited usefulness in diagnosis.
ARCHIVOS ESPANOLES DE UROLOGIA
(2021)