Article
Virology
Chu Wang, Lina Meng, Jialin Wang, Kaikai Zhang, Sizhu Duan, Pengyu Ren, Yingzhe Wei, Xinyu Fu, Bin Yu, Jiaxin Wu, Xianghui Yu
Summary: Human SAMHD1 restricts lentiviruses at the reverse transcription step through its dNTPase activity. Feline and bovine SAMHD1 were mainly localized to the nucleus but retained antiviral function in the cytoplasm, especially against Vpx-encoding SIV and HIV-2. Cytoplasmic feline SAMHD1 demonstrated resistance to Vpx-induced degradation, similar to human SAMHD1, indicating potential for more effective lentiviral suppression.
Article
Medicine, Research & Experimental
Takuya Tada, Ju-Yi Peng, Belinda M. Dcosta, Nathaniel R. Landau
Summary: Currently authorized COVID-19 vaccines induce both humoral and cellular responses targeting the spike protein of SARS-CoV-2, but the specific roles of antibodies and T cells in protection are not well understood. In this study, a T cell-only vaccine was developed by targeting dendritic cells with a lentiviral vector expressing single CD8+ T cell epitopes of the viral nucleocapsid, spike, and ORF1. Immunization with this vaccine induced antigen-specific CD8+ T cell proliferation and resulted in significant reduction in virus load upon live virus challenge, including against the Omicron variant. This highlights the importance of T cell responses in vaccine-mediated protection and suggests that incorporating additional T cell epitopes into current vaccines could enhance their efficacy and broaden protection.
Article
Oncology
Waaqo Daddacha, Dominique Monroe, Kristen Carver, Edidiong R. Usoro, Ahmet Alptekin, Hongyan Xu, Satoru Osuka, Ali S. Arbab, Daitoku Sakamuro
Summary: Glioblastoma is a common and lethal primary brain tumor with limited treatment options. Depleting the SAMHD1 protein can enhance the sensitivity of glioblastoma to radiation and temozolomide, and viral protein X (Vpx) may serve as a therapeutic tool.
Article
Microbiology
Hangil Kim, Hanako Shimura, Kae Sueda, Chikara Masuta
Summary: RNA silencing is an important defense mechanism of host plants against viral infection. Cucumber mosaic virus encodes a protein called 2b, which suppresses RNA silencing. This study found that the phosphorylation of 2b at position 28 negatively regulates its RNA silencing suppression activity by affecting its nuclear localization. The study also discovered that 2b's transport between the nucleus and cytoplasm is similar to that of Argonaute proteins, suggesting that 2b manipulates its nucleocytoplasmic trafficking to track down its targets in the RNA silencing pathway. The efficient RNA silencing suppression activity of 2b is maintained by a balance of phosphorylation and dephosphorylation and is coupled to importin/exportin-mediated shuttling between the nucleus and cytoplasm.
Article
Virology
Luis Adrian De Jesus-Gonzalez, Selvin Noe Palacios-Rapalo, Jose Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Carlos Daniel Cordero-Rivera, Bulmaro Cisneros, Ana Lorena Gutierrez-Escolano, Rosa Maria del Angel
Summary: Recent evidence suggests that nuclear elements play a role in the replication of Flaviviruses, with certain viral proteins being imported into the nucleus. Furthermore, nuclear localization of these proteins has been observed in infected cells. The presence of ZIKV NS3 in the nucleus has been investigated, showing that NS3 is imported into the nucleus via the importin pathway and exported back to the cytoplasm.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Min Chen, Xue Yang, Haiyang Liu, Jun Wan
Summary: In this study, a functional bipartite nuclear localization signal (bNLS) in the ANKRD11 gene was identified. It was found that this bNLS is responsible for the nuclear import of ANKRD11 and certain clinical variants of this signal may be related to disease development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Agata Lampart, Katarzyna Dominika Sluzalska, Aleksandra Czyrek, Aleksandra Szerszen, Jacek Otlewski, Antoni Wiedlocha, Malgorzata Zakrzewska
Summary: The nuclear localization sequence (NLS) in fibroblast growth factor 1 (FGF1) is not required for its intracellular anti-apoptotic activity in differentiated cells, suggesting that the mechanism of stress response differs according to the level of cell differentiation.
Article
Biotechnology & Applied Microbiology
Xiangshuo Kong, Guanping Chen, Jiale Li, Yuedong Li, Xiaofeng Wu
Summary: The relationship between viral-induced nuclear vesicles and the Bm5 protein of BmNPV is unclear. It is found that the Bm5 protein of BmNPV is involved in the formation of nuclear vesicle-like structures, which affects the production of occlusion-derived virions (ODVs) and occlusion bodies (OBs). The formation of nuclear vesicle-like structures is independent of the transport or recruitment of major envelope proteins of ODVs, such as P74 and Bm14.
JOURNAL OF GENERAL VIROLOGY
(2023)
Article
Genetics & Heredity
Leslie M. Stevens, Goheun Kim, Theodora Koromila, John W. Steele, James McGehee, Angelike Stathopoulos, David S. Stein
Summary: The development of a new photo-N-degron allows for light-dependent degradation of proteins in vivo, providing a potential tool for optogenetic studies. Comparisons with other light-dependent degrons show that the photo-N-degron and the B-LID domain are effective in inducing light-dependent protein degradation, while the photosensitive degron is less effective. Careful selection of inducible degrons is crucial for studying protein function in vivo.
Article
Cell Biology
Xinxin Chi, Wei Jin, Xue Bai, Xiaohong Zhao, Jing Shao, Jiaqi Li, Qinli Sun, Bing Su, Xiaohu Wang, Xuexian O. Yang, Chen Dong
Summary: The study found that RORα is highly expressed in active UC patients, especially in those non-responsive to anti-TNF treatment. RORα plays a crucial role in promoting IBD development in CD4(+) T cells by regulating T cell infiltration, inhibiting T cell apoptosis, and promoting mTORC1 activation. This indicates that the RORα-mTORC1 axis may be a potential therapeutic target for human patients with IBD.
Article
Cell Biology
Haiyuan Mu, Ting Zhang, Ying Yang, Danru Zhang, Jie Gao, Junhong Li, Liang Yue, Dengfeng Gao, Bingbo Shi, Yue Han, Liang Zhong, Xinze Chen, Zhen-Bo Wang, Zhen Lin, Ming-Han Tong, Qing-Yuan Sun, Yun-Gui Yang, Jianyong Han
Summary: m(6)A modification plays essential roles in follicle development and oocyte growth, regulating oocyte meiosis by enhancing RNA stability.
CELL DEATH & DISEASE
(2021)
Article
Plant Sciences
Lisen Liu, Zhaohan Wang, Jianing Li, Ye Wang, Jiachen Yuan, Jingjing Zhan, Peng Wang, Yongjun Lin, Fuguang Li, Xiaoyang Ge
Summary: The study identified Vd424Y as an important effector protein of V. dahliae that targets the host nucleus to regulate and activate effector-triggered immunity in plants, enhancing resistance to pathogens.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wenyuan Li, Wei Li, Xun Li, Luwen Wang, Yao Wang
Summary: This study investigated the role of G3BP1 in regulating ferroptosis in hepatocytes during ALF by affecting P53's entry into the nucleus. It was found that promoting G3BP1 expression could inhibit P53 entry by binding to its nuclear localization sequence. Inhibition of P53's binding to the promoter region of the SLC7A11 gene weakened the repression of SLC7A11 transcription. As a result, the SLC7A11-GSH-GPX4 antiferroptotic pathway was activated, leading to inhibition of ferroptosis in ALF hepatocytes.
Article
Optics
Yucheng Wang
Summary: In this study, we investigated the localization phenomena in a one-dimensional periodically kicked quasiperiodic optical Raman lattice using fractal dimensions. We demonstrated a rich phase diagram including pure extended, critical, and localized phases, as well as the fragility of phase boundaries and the phenomenon of reentrant localization transition.
Article
Medical Laboratory Technology
Jia Liao, Xingyu Su, Miao Wang, Lucen Jiang, Xi Chen, Zixi Liu, Guoqing Tang, Li Zhou, Hongmei Li, Xiuxiu Lv, Jun Yin, Huadong Wang, Yiyang Wang
Summary: This study discovered that the level of CHIP gradually decreased in the heart after septic model construction. The decline in CHIP expression was related to c-Jun activation that inhibited CHIP transcription. CHIP promoted the degradation of KPNA2 through polyubiquitination, reducing the production of proinflammatory cytokines and attenuating sepsis-induced cardiac dysfunction.
TRANSLATIONAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Xiaoling Hong, Siying Li, Wanying Li, Min Xie, Zhentong Wei, Haoran Guo, Wei Wei, Songling Zhang
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Haoran Guo, Yan Li, Guanchen Liu, Yunhe Jiang, Siyu Shen, Ran Bi, Honglan Huang, Tong Cheng, Chunxi Wang, Wei Wei
NATURE COMMUNICATIONS
(2019)
Article
Microbiology
Siying Li, Xiaoling Hong, Zhentong Wei, Min Xie, Wanying Li, Guanchen Liu, Haoran Guo, Jiaxin Yang, Wei Wei, Songling Zhang
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Pharmacology & Pharmacy
Dong Wang, Haoran Guo, Huahong Yang, Dongyin Wang, Pujun Gao, Wei Wei
FRONTIERS IN PHARMACOLOGY
(2019)
Article
Pharmacology & Pharmacy
Yunhe Jiang, Shunan Liu, Siyu Shen, Haoran Guo, Honglan Huang, Wei Wei
ANTIVIRAL RESEARCH
(2020)
Article
Oncology
Ran Bi, Yuyou Deng, Chao Tang, Lei Xuan, Bo Xu, Yujun Du, Chunxi Wang, Wei Wei
Article
Pharmacology & Pharmacy
Hong Zhang, Min Wu, Xiaoxue Zhu, Cuiyun Li, Xiaojiao Li, Jixuan Sun, Chengjiao Liu, Quan Liu, Wei Wei, Junqi Niu, Yanhua Ding
FRONTIERS IN PHARMACOLOGY
(2020)
Article
Gastroenterology & Hepatology
Fei Kong, Xiaoyu Wen, Xiaofeng Wen, Xiaozhong Wang, Guicheng Wu, Shide Lin, Li Wang, Huichun Xing, Xuebing Yan, Sujun Zheng, Qin Ning, Zheng Wang, Liaoyun Zhang, Jianmei Lin, Zhaowei Tong, Chengyu Huang, Minghua Su, Lixin Tong, Jidong Jia, Yongning Xin, Qingjing Zhu, Jing Wang, Li Chen, Xiaowen Li, Xuegang Wu, Duan Niu, Quan Liu, Wei Wei, Yuexin Zhang, Guangming Li, Junqi Niu
Summary: The study demonstrated that the combination therapy of seraprevir and sofosbuvir is effective and well-tolerated for treating Chinese patients with chronic HCV infection, even those with a history of interferon treatment.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Review
Medicine, Research & Experimental
Heming Ma, Chang Wang, Xu Liu, Mengru Zhan, Wei Wei, Junqi Niu
Summary: SHC1 protein, as a crucial member of the SHC protein family, regulates cell functions and is involved in the occurrence and progression of liver diseases. It has been demonstrated to be closely associated with acute and chronic liver injuries, liver fibrosis, and hepatocellular carcinoma.
Article
Oncology
Jingyu Peng, Ming Yang, Ran Bi, Yueyuan Wang, Chunxi Wang, Xue Wei, Zhihao Zhang, Xiao Xie, Wei Wei
Summary: The study found that CDK7 inhibitors are more effective in TNBC cells with p53 mutations, with the treatment specifically downregulating mutated p53 expression and suppressing cell proliferation. Targeting CDK7 could be an effective approach for treating TNBC with p53 mutation.
FRONTIERS IN ONCOLOGY
(2021)
Letter
Virology
Zhe Zhang, Haoran Guo, Jing Wang, Yan Li, Yanhang Gao, Quan Liu, Junqi Niu, Wei Wei
Article
Microbiology
Yan Li, Siyu Shen, Haoran Guo, Zhe Zhang, Lili Zhang, Qingran Yang, Yanhang Gao, Junqi Niu, Wei Wei
Summary: This study investigated the impact of enteroviral infection on L1 mobility, revealing that different enteroviruses can block L1 transposition. Additionally, viral proteins EV-D68 2A, 3A, 3C, and EV-A71 ORF2p were identified as inhibitors of L1 activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Shunan Liu, Xia Cao, Haoran Guo, Wei Wei
Summary: The study found that zinc ions have significant antiviral activity against EV-D68 infection in vitro, suppressing viral replication and infectiousness. Zinc has the potential to be an effective strategy for treating EV-D68 infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Materials Science, Biomaterials
Jinrui Zhang, Yao Sun, Qian Qu, Bo Li, Lili Zhang, Rui Gu, Jianlin Zuo, Wei Wei, Chao Ma, Lei Liu, Kai Liu, Jingjing Li, Hongjie Zhang
Summary: A novel nanotherapeutic formulation was developed through noncovalent assembly of an engineered IL-1Ra chimera protein, which exhibited improved bioavailability and long-lasting anti-inflammatory effects for gouty arthritis treatment. This approach could potentially be adapted for other therapeutics to create long-acting formulations.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Biochemistry & Molecular Biology
Yuyou Deng, Ran Bi, Haoran Guo, Jiaxin Yang, Yujun Du, Chunxi Wang, Wei Wei
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2019)