期刊
PLOS ONE
卷 8, 期 7, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0069647
关键词
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资金
- French National Space Agency (CNES)
- National Research Agency [ANR NT09-632666]
- French Ministry of Higher Education and Research
- Lorraine University
- Lorraine area
- BELSPO/PRODEX/ESA [42-000-90-380]
- National Research Fund of Luxembourg
- CNES
DNA polymerase m is involved in DNA repair, V(D)J recombination and likely somatic hypermutation of immunoglobulin genes. Our previous studies demonstrated that spaceflight conditions affect immunoglobulin gene expression and somatic hypermutation frequency. Consequently, we questioned whether Pol mu expression could also be affected. To address this question, we characterized Pol mu of the Iberian ribbed newt Pleurodeles waltl and exposed embryos of that species to spaceflight conditions or to environmental modifications corresponding to those encountered in the International Space Station. We noted a robust expression of Pol mu mRNA during early ontogenesis and in the testis, suggesting that Pol mu is involved in genomic stability. Full-length Pol mu transcripts are 8-9 times more abundant in P. waltl than in humans and mice, thereby providing an explanation for the somatic hypermutation predilection of G and C bases in amphibians. Pol mu transcription decreases after 10 days of development in space and radiation seem primarily involved in this down-regulation. However, space radiation, alone or in combination with a perturbation of the circadian rhythm, did not affect Pol mu protein levels and did not induce protein oxidation, showing the limited impact of radiation encountered during a 10-day stay in the International Space Station.
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