Article
Biochemistry & Molecular Biology
Giulia Capitoli, Isabella Piga, Vincenzo L'Imperio, Francesca Clerici, Davide Leni, Mattia Garancini, Gabriele Casati, Stefania Galimberti, Fulvio Magni, Fabio Pagni
Summary: Fine-needle aspiration biopsies (FNA) are commonly used to exclude the malignant nature of thyroid nodules, but a significant proportion of cases are indeterminate. A study found that matrix-assisted laser-desorption ionization mass spectrometry imaging (MALDI-MSI) can improve the diagnostic performance of FNA by separating benign and malignant cell areas. However, the performance of the model is affected by poor spectra and noise.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Shujun Xia, Yilai Chen, Weiwei Zhan, Wei Zhou
Summary: This study compared the effectiveness of ultrasound-guided fine-needle aspiration and capillary sampling with different gauge needles for cervical lymph node biopsy. Results showed that 22G fine-needle aspiration and 22G capillary sampling were more effective in sampling adequacy and diagnostic efficiency compared to 25G fine-needle aspiration. The study suggests that 22G fine-needle aspiration and 22G capillary sampling may be more suitable for diagnosing cervical lymph nodes.
FRONTIERS IN ONCOLOGY
(2021)
Article
Otorhinolaryngology
Michael R. Papazian, Jared C. Dublin, Kepal N. Patel, Thaira Oweity, Adam S. Jacobson, Tamar C. Brandler, Babak Givi
Summary: This study analyzed the clinical outcomes of repeat fine-needle aspiration (FNA) biopsy and genomic classifier results in indeterminate thyroid nodules (ITNs). The results showed that repeat FNA helped reclassify nodules as benign, avoiding the need for diagnostic surgery. The risk of high-risk malignancy in ThyroSeq-positive nodules with repeat indeterminate cytology was low.
OTOLARYNGOLOGY-HEAD AND NECK SURGERY
(2023)
Article
Otorhinolaryngology
Tobias Todsen, Finn Noe Bennedbaek, Katalin Kiss, Laszlo Hegedus
Summary: Fine needle aspiration biopsy (FNAB) is crucial for evaluating thyroid nodules with suspicious features, and the use of US-guidance is important for real-time visualization of the needle. Collaboration among different specialties is essential for a standardized and meticulous technique to reduce nondiagnostic specimens and false-negative results.
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2021)
Article
Health Care Sciences & Services
Michael Osseis, Georges Jammal, Daniel Kazan, Roger Noun
Summary: This study compared the diagnostic accuracy of fine needle aspiration cytology (FNAC) and ultrasound in thyroid nodule diagnosis. The results showed that FNAC had a higher sensitivity than ultrasound, while ultrasound had a higher specificity than FNAC. Combining these two methods can improve diagnostic accuracy.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Medicine, General & Internal
Sule Canberk, Ana Rita Lima, Mafalda Pinto, Valdemar Maximo
Summary: The importance of epigenetic alterations in cancer research has attracted researchers' attention, with a focus on thyroid neoplasms and cytology materials. These studies are expected to provide a solid foundation for validating patterns of epigenetic alterations and their potential use in clinical management in the future.
FRONTIERS IN MEDICINE
(2021)
Article
Endocrinology & Metabolism
Ilenia Pirola, Elena Di Lodovico, Claudio Casella, Letizia Pezzaioli, Paolo Facondo, Alberto Ferlin, Davide Lombardi, Carlo Cappelli
Summary: This study aimed to evaluate the impact of thyroid scintigraphy on the clinical management of patients referred for FNA. The results showed that in patients with TSH levels <= 1.5 mIU/L, using thyroid scintigraphy can help identify hyperfunctioning nodules and avoid unnecessary FNAs.
CLINICAL ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Pengfei Luo, Xiali Mu, Wei Ma, Dahai Jiao, Peixin Zhang
Summary: This study aimed to demonstrate the non-inferiority of thyroid fine needle aspiration (FNA) without a stylet (S-) compared to FNA with a stylet (S+) in terms of specimen sampling. The results showed no significant difference in specimen adequacy between the two methods. Therefore, FNA without a stylet is a feasible approach for thyroid FNA.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Dianna L. Ng, Annemieke van Zante, Ann Griffin, Nancy K. Hills, Britt-Marie Ljung
Summary: This study retrospectively reviewed the UCSF pathology database for thyroid FNABs, analyzing a large cohort of patients with long-term follow-up to determine the risk of thyroid malignancy for different diagnostic categories. The results showed that patients with initial benign or unsatisfactory biopsy results are unlikely to develop thyroid malignancy or die of thyroid cancer, highlighting the high accuracy of FNAB in detecting malignancy.
Article
Oncology
Yongchun Wang, Brittany A. McKelvey, Zhiyang Liu, Lisa Rooper, Leslie M. Cope, Martha A. Zeiger, Christopher B. Umbricht
Summary: The newly developed 5-gene isoform expression panel can distinguish between benign and malignant thyroid tumors, and may also help in distinguishing benign from malignant thyroid nodules in the context of the new NIFTP subtype.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2021)
Article
Biochemical Research Methods
Isabella Piga, Giulia Capitoli, Francesca Clerici, Allia Mahajneh, Virginia Brambilla, Andrew Smith, Davide Leni, Vincenzo L'Imperio, Stefania Galimberti, Fabio Pagni, Fulvio Magni
Summary: Fine needle aspiration is the primary method for diagnosing thyroid nodules. Matrix-assisted laser desorption/ionization mass spectrometry imaging has been effective in distinguishing proteomic profiles of benign and malignant thyroid FNAs. Ex vivo FNAs could provide a valuable alternative with increased sample size and statistical power.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Oncology
Huang Chen, Aiping Song, Ye Wang, Yifan He, Jie Tong, Jinxi Di, Chun Li, Zhongren Zhou, Xiaopin Cai, Dingrong Zhong, Jiping Da
Summary: The study demonstrated that combining fine-needle aspiration cytology with BRAF(V600E) mutation testing can improve the accuracy of papillary thyroid cancer diagnosis, especially for thyroid nodules with indeterminate or nondiagnostic cytology.
Article
Pathology
Michael Rechter, Sivan Saraph, Hector Cohen, Ohad Ronen
Summary: This study compared the effects of different needle sizes (23-, 25-, and 27-gauge) on the non-diagnostic (ND) rates of thyroid fine-needle aspiration biopsy (FNAB). The results showed that the 25-gauge needle had a significantly lower ND rate compared to the 27-gauge needle, and increased nodule size was associated with a reduced ND rate.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Otorhinolaryngology
Seon Min Jung, Hye Ryoung Koo, Ki Seok Jang, Min Sung Chung, Chang Myeon Song, Yong Bae Ji, Jeong Seon Park, Kyung Tae
Summary: Ultrasound-guided CNB is more effective than rFNA for repeat biopsy of thyroid nodules with inconclusive initial FNA results, especially for patients with categories I and III, but showed no significant differences for category IV nodules.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2021)
Article
Computer Science, Artificial Intelligence
Nguyen Thanh Duc, Yong-Moon Lee, Jae Hyun Park, Boreom Lee
Summary: The study utilized artificial intelligence for automated classification of Papillary Thyroid Carcinoma cell clusters, achieving high accuracy and reliability through segmentation of cytological images and deep learning model pre-training and fine-tuning.
EXPERT SYSTEMS WITH APPLICATIONS
(2022)
Review
Spectroscopy
Marco Borso, Patrizia Agretti, Riccardo Zucchi, Alessandro Saba
Summary: Thyroid hormones play a crucial role in development, growth, and energy metabolism. The advancement in mass spectrometry has enabled the easier and more accurate quantification of thyroid hormones, making it a valuable tool in clinical diagnostics and research.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Endocrinology & Metabolism
G. Rotondo Dottore, L. Torregrossa, G. Lanzolla, S. Mariotti, F. Menconi, P. Piaggi, L. Cristofani Mencacci, C. Posarelli, M. N. Maglionico, I. Dallan, M. Figus, M. Nardi, C. Marcocci, F. Basolo, M. Marino
Summary: The majority of GO patients have mononuclear cell infiltrates in the orbit, with a small percentage exclusively represented by plasmacells. CD4 cells play a major role in GO activity. These findings may contribute to a better understanding of the pathogenesis of GO.
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
(2022)
Article
Oncology
Paola Vignali, Agnese Proietti, Elisabetta Macerola, Anello Marcello Poma, Liborio Torregrossa, Clara Ugolini, Alessio Basolo, Antonio Matrone, Teresa Rago, Ferruccio Santini, Rossella Elisei, Gabriele Materazzi, Fulvio Basolo
Summary: This study retrospectively analyzed a large cohort of NIFTPs and compared the clinico-pathological features of solitary and multifocal lesions. The results showed that NIFTPs often coexisted with other thyroid tumors, but no significant clinical-pathological differences were observed between them. Therefore, careful monitoring of the contralateral lobe is still important.
Review
Oncology
Elisabetta Macerola, Anello Marcello Poma, Paola Vignali, Agnese Proietti, Clara Ugolini, Liborio Torregrossa, Alessio Basolo, Rossella Elisei, Ferruccio Santini, Fulvio Basolo
Summary: Predictive biomarkers in molecular pathology identify patients who are likely to respond to targeted drugs, with the availability of new selective targeted drugs changing diagnostic strategies for molecular characterization of tumors.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Liborio Torregrossa, Anello Marcello Poma, Elisabetta Macerola, Teresa Rago, Paola Vignali, Rossana Romani, Agnese Proietti, Iose Di Stefano, Giuditta Scuotri, Clara Ugolini, Alessio Basolo, Alessandro Antonelli, Gabriele Materazzi, Ferruccio Santini, Fulvio Basolo
Summary: The Italian Consensus for the Classification and Reporting of Thyroid Cytology (ICCRTC) includes six diagnostic categories, each indicating a different risk of malignancy. This monocentric retrospective study aimed to evaluate the distribution of ICCRTC classes and their cytohistologic correlations. The results showed that nondiagnostic and benign categories were the most common, but surprisingly had high malignancy rates. The suspicious and malignant categories had high specificity for malignancy, and the ICCRTC was reliable in identifying aggressive histotypes of thyroid carcinoma.
CANCER CYTOPATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Elisabetta Macerola, Agnese Proietti, Anello Marcello Poma, Paola Vignali, Rebecca Sparavelli, Alessandro Ginori, Alessio Basolo, Rossella Elisei, Ferruccio Santini, Fulvio Basolo
Summary: This study investigates the diagnostic utility of Trk immunohistochemistry in identifying NTRK rearrangements. The results show that screening based on IHC analysis has limited sensitivity and specificity in identifying NTRK rearranged tumors. Therefore, alternative detection strategies should be considered for thyroid cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Laura Giusti, Marta Tesi, Federica Ciregia, Lorella Marselli, Lorenzo Zallocco, Mara Suleiman, Carmela De Luca, Silvia Del Guerra, Mariachiara Zuccarini, Marco Trerotola, Decio L. Eizirik, Miriam Cnop, Maria R. Mazzoni, Piero Marchetti, Antonio Lucacchini, Maurizio Ronci
Summary: Metformin was found to alleviate human beta-cell stress induced by pro-inflammatory cytokines, leading to proteome modifications in human islets.
Article
Endocrinology & Metabolism
Laura Croce, Luca Chiovato, Massimo Tonacchera, Elena Petrosino, Maria Laura Tanda, Mariacarla Moleti, Flavia Magri, Antonella Olivieri, Elizabeth N. Pearce, Mario Rotondi
Summary: The study aimed to review the available evidence from meta-analyses on iodine supplementation during pregnancy in areas with mild-moderate deficiency. The findings suggest a U-shaped correlation between iodine status and maternal and neonatal outcomes, but the results of intervention trials are inconclusive. More high-quality studies are needed to further clarify this issue.
REVIEWS IN ENDOCRINE & METABOLIC DISORDERS
(2023)
Article
Biochemistry & Molecular Biology
Lorenzo Zallocco, Roberto Silvestri, Federica Ciregia, Alessandra Bonotti, Riccardo Marino, Rudy Foddis, Antonio Lucacchini, Laura Giusti, Maria Rosa Mazzoni
Summary: This study found that the levels of prosaposin and SULF-1 in pleural effusion may be promising biomarkers for differentiating between mesothelioma and non-malignant pleural disease. However, more patients need to be enrolled to confirm the usefulness of these soluble proteins in differentiating mesothelioma pleural effusions from those linked to lung cancer.
Article
Endocrinology & Metabolism
Daniele Sgro, Alessandro Brancatella, Giuseppe Greco, Liborio Torregrossa, Paolo Piaggi, Nicola Viola, Teresa Rago, Fulvio Basolo, Riccardo Giannini, Gabriele Materazzi, Rossella Elisei, Ferruccio Santini, Francesco Latrofa
Summary: Prognosis is good for papillary thyroid carcinoma, noninvasive follicular thyroid neoplasia with papillary-like nuclear features, and follicular thyroid carcinoma, but poor for poorly differentiated thyroid carcinoma and anaplastic thyroid carcinoma. Ultrasound can be helpful in ruling out tall cell variant papillary thyroid carcinoma.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Claudio Alimenti, Mariacaterina Lianza, Fabiana Antognoni, Laura Giusti, Onelia Bistoni, Luigi Liotta, Cristina Angeloni, Giulio Lupidi, Daniela Beghelli
Summary: Olive pomace (OP), a by-product of olive oil production, contains health-promoting bioactive compounds. Different OP batches showed variations in phenolic compounds and antioxidant activities. The aqueous extract of OP exhibited potential anti-inflammatory properties different from the metabolome of OP fractions.
Article
Biology
Daniela Beghelli, Lorenzo Zallocco, Cristina Angeloni, Onelia Bistoni, Maurizio Ronci, Clarita Cavallucci, Maria Rosa Mazzoni, Anna Nuccitelli, Chiara Catalano, Silvana Hrelia, Antonio Lucacchini, Laura Giusti
Summary: A placebo-controlled study was conducted on 16 show jumping horses to evaluate the effects of a dietary supplement containing Verbascum thapsus, Curcuma longa, and Boswellia serrata extracts. The supplementation led to modulation of proteins and downregulation of proinflammatory cytokines in the horses. The study suggests that this phytotherapy can strengthen the innate immune responses and alleviate inflammation in sport horses.
Review
Medicine, General & Internal
Paola Vignali, Elisabetta Macerola, Anello Marcello Poma, Rebecca Sparavelli, Fulvio Basolo
Summary: Thyroid cancer is the most common malignancy of the endocrine system. Accurately classifying cytological specimens as benign or malignant based on cytomorphological features is a challenge for cytopathologists. Recent studies have identified two molecular profiles of thyroid tumors, RAS-like and BRAF(V600E)-like. Molecular tests play an important role in guiding patients' management.
Review
Endocrinology & Metabolism
Fulvio Basolo, Elisabetta Macerola, Anello Marcello Poma, Liborio Torregrossa
Summary: The 5th edition of the WHO classification of endocrine tumors, released in 2022, introduces several novelties in the nomenclature and histopathological diagnosis of follicular-derived thyroid neoplasms. Tumor types are now categorized into benign tumors, low-risk neoplasms, and malignant neoplasms based on prognostic risk. A grading system for differentiated thyroid carcinomas has been implemented for better tumor stratification. The review critically discusses the practical effects on diagnostic pathology and the expected clinical implications of the new classification scheme.
Article
Multidisciplinary Sciences
Cristina Di Primio, Paola Quaranta, Marianna Mignanelli, Giacomo Siano, Matteo Bimbati, Arianna Scarlatti, Carmen Rita Piazza, Piero Giorgio Spezia, Paola Perrera, Fulvio Basolo, Anello Marcello Poma, Mario Costa, Mauro Pistello, Antonino Cattaneo
Summary: COVID-19 infection is associated with the aggravation of Alzheimer's disease, and this study provides evidence that SARS-CoV-2 infection induces phosphorylation of Tau protein at pathological epitopes, leading to aggregation and altered subcellular localization.