Article
Cardiac & Cardiovascular Systems
Juan Francisco Aranda, Ana Perez-Garcia, Marta Torrecilla-Parra, Mario Fernandez-de Frutos, Yolanda Martin-Martin, Pedro A. Mateos-Gomez, Virginia Pardo-Marques, Rebeca Busto, Cristina M. Ramirez
Summary: Hypoxia plays a crucial role in the maintenance of atherosclerotic lesions. miR-199a-5p and ABCA1 are inversely regulated by hypoxia and Ac-LDL, and their interaction can control macrophage cholesterol efflux during the onset of atherosclerosis.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Zhexiao Zhang, Yunmei Qiu, Wanzhi Li, Anyang Tang, Hang Huang, Wanyi Yao, Huawen Li, Tangbin Zou
Summary: It is widely believed that the deposition of foam cells in the arterial wall is the main cause of atherosclerosis. However, our experimental results suggest that astaxanthin can promote cholesterol efflux by modulating ATP-binding cassette subfamily A member 1, thereby inhibiting foam cell formation and preventing the occurrence and development of atherosclerosis.
Article
Multidisciplinary Sciences
Jere P. Segrest, Chongren Tang, Hyun D. Song, Martin K. Jones, W. Sean Davidson, Stephen G. Aller, Jay W. Heinecke
Summary: By using simulations, the authors of this study uncover the mechanism by which ATP-binding cassette transporter A1 (ABCA1) functions in extracting lipids from the outer face of the plasma membrane and facilitating the production of high density lipoprotein (HDL). Contrary to existing models, their findings suggest that ABCA1 removes lipids from the plasma membrane and forces them through its gateway and annulus into an elongated hydrophobic tunnel. This study provides important insights into the function of ABCA1 and presents a potential target for further research.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Hye Youn Sung, Eun Nam Choi, Jihye Han, Yun Ju Chae, Sun-Wha Im, Hee-Sun Kim, Eun-Mi Park, Jung-Hyuck Ahn
Summary: Research suggests that ischemic preconditioning reduces brain ischemia-reperfusion injury by downregulating the expression of miR-33-5p and miR-135b-5p, which in turn protects neurons from apoptosis induced by oxygen-glucose deprivation. Inhibition of these two miRNAs significantly increases mRNA and protein levels of ABCA1 and reduces activation of the mitochondria-dependent apoptosis pathway.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Fei Xu, Li Shen, Han Chen, Rui Wang, Tongtong Zang, Juying Qian, Junbo Ge
Summary: Inflammation plays a crucial role in atherosclerosis, and circRNA circDENND1B has been identified as a novel mediator of atherosclerosis in mice. Upregulation of circDENND1B alleviates foam cell formation and promotes cholesterol efflux, suggesting its potential as a therapeutic target in atherosclerosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Medical Laboratory Technology
Shun Zhang, Lu Li, Jie Wang, Tingting Zhang, Ting Ye, Shuai Wang, Dongming Xing, Wujun Chen
Summary: lncRNAs, as a unique group of RNA transcripts longer than 200 nucleotides, regulate the roles of ABCA1 and ABCG1 in cholesterol efflux, influencing the development of atherosclerosis.
CLINICA CHIMICA ACTA
(2021)
Article
Medicine, Research & Experimental
Noemi Rotllan, Xinbo Zhang, Alberto Canfran-Duque, Leigh Goedeke, Raquel Grinan, Cristina M. Ramirez, Yajaira Suarez, Carlos Fernandez-Hernando
Summary: miR-148a-3p plays an important regulatory role in the progression of atherosclerosis. Inhibition of miR-148a can reduce plaque size and promote plaque stability, possibly through regulating macrophage cholesterol efflux and the inflammatory response.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Gopalan Gnanaguru, Alexandre Wagschal, Justin Oh, Kahira L. Saez-Torres, Tong Li, Ryan E. Temel, Mark E. Kleinman, Anders M. Naar, Patricia A. D'Amore
Summary: Elevated miR-33 levels were found to be associated with AMD, and targeting miR-33 can decrease pathological changes in the RPE cell layer by regulating ABCA1 expression and cholesterol efflux.
Article
Pharmacology & Pharmacy
Cheng Zhang, Xuewen Wu, Pengmin Shi, Hongyu Ma, Fei Fang, Qianlang Feng, Shuang Zhao, Ruipu Zhang, Jinyuan Huang, Xinting Xu, Weilie Xiao, Guang Cao, Xu Ji
Summary: In this study, it was found that diterpenoids from the traditional Chinese medicine Callicarpa rubella could effectively inhibit oxidized low-density lipoprotein-induced macrophage foam cell formation by promoting cholesterol efflux, suggesting their potential as drugs for the treatment of atherosclerosis.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Medical Laboratory Technology
Wujun Chen, Lu Li, Jie Wang, Renshuai Zhang, Tingting Zhang, Yudong Wu, Shuai Wang, Dongming Xing
Summary: ABCA1 plays a central role in connecting cholesterol efflux and apoptotic cell clearance by regulating the release and expression of various ligands to promote efferocytosis.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Fengqing Zhu, Guanglei Chang, Xiaoqiong Tang, Lingyun Gao, Nan Zhang
Summary: Doxorubicin (DOX) is used for cancer treatment but can cause cardiovascular toxicity. This study examined whether DOX promotes atherosclerosis by impairing cardiac function. The results showed that DOX increased miR-33 expression and reduced the protein level of ATP binding cassette transporter A1 (ABCA1). DOX also suppressed cholesterol efflux, especially under high-cholesterol conditions. Transfection of miR-33 mimics or inhibitors into cells respectively decreased or increased ABCA1 protein expression and regulated intracellular lipid accumulation. Overall, DOX upregulates miR-33 to suppress ABCA1 expression, leading to intracellular lipid deposition in macrophages, which is an early sign of atherosclerosis. This study provides new insights for the clinical observation and evaluation of DOX side effects.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cardiac & Cardiovascular Systems
Zhen-Wang Zhao, Min Zhang, Gang Wang, Jin Zou, Jia-Hui Gao, Li Zhou, Xiang-Jun Wan, Da-Wei Zhang, Xiao-Hua Yu, Chao-Ke Tang
Summary: Astragalin protects against atherosclerosis by promoting ABCA1- and ABCG1-mediated cholesterol efflux and inhibiting the release of proinflammatory mediators. Its mechanism of action involves upregulating the expression of ABCA1 and ABCG1, reducing TLR4 surface levels, and inhibiting NF-kappa B nuclear translocation.
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2021)
Review
Nutrition & Dietetics
Malgorzata Sidorkiewicz
Summary: The maintenance of cholesterol homeostasis involves complicated processes such as regulation of cholesterol synthesis, dietary uptake, and bile acid synthesis and excretion. Reverse cholesterol transport, mediated by high-density lipoproteins, plays a vital role in transferring cholesterol from non-hepatic cells to the liver for excretion. MicroRNA-33 has been identified as a key regulator of cholesterol homeostasis, impacting both cellular cholesterol efflux and bile metabolism in the liver. Understanding these mechanisms could lead to new perspectives in microRNA-targeted treatment for cholesterol imbalance.
Article
Biochemistry & Molecular Biology
Xiao-Dan Xia, Xiao-Hua Yu, Ling-Yan Chen, Song-lin Xie, Yao-Guang Feng, Rui-Zhe Yang, Zhen-Wang Zhao, Heng Li, Gang Wang, Chao-Ke Tang
Summary: The study found that both MYOCD and ABCA1 expression were significantly decreased in atherosclerotic patient aortas. Knockdown of MYOCD inhibited ABCA1 expression in human aortic vascular smooth muscle cells (HAVSMCs), leading to reduced cholesterol efflux and increased intracellular cholesterol contents.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Review
Pharmacology & Pharmacy
Masatsune Ogura
Summary: Homozygotes with loss-of-function mutations in ABCA1 develop Tangier disease, which is characterized by significantly reduced or absent blood high-density lipoprotein (HDL) cholesterol. ABCA1 was identified as a key player in HDL production based on studies involving ABCA1-deficient mice. While the activation of ABCA1 and promotion of cholesterol efflux could potentially lead to the development of anti-atherosclerotic drugs, it is challenging to consider simple ABCA1 activation or cholesterol efflux promotion as an effective strategy for intracellular cholesterol homeostasis. Currently, there is no evidence supporting the preventive effect of HDL-increasing drugs that enhance ABCA1 expression on atherosclerotic disease in humans. However, in situations where intracellular cholesterol homeostasis is disrupted by inflammation, aging, or metabolic abnormalities, restoring ABCA1 expression and cholesterol efflux may be a useful strategy.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2022)
Review
Medical Laboratory Technology
Yudong Wu, Qian Li, Renshuai Zhang, Xiaoli Dai, Wujun Chen, Dongming Xing
Summary: MicroRNAs, approximately 20-24 nucleotides in length, are considered reliable diagnostic and prognostic biomarkers for various diseases due to their reflection of disease progression and drug resistance. They play a crucial role in the diagnosis, prognosis, and treatment response prediction of diseases, potentially improving cure rates.
CLINICA CHIMICA ACTA
(2021)
Review
Medical Laboratory Technology
Shun Zhang, Lu Li, Jie Wang, Tingting Zhang, Ting Ye, Shuai Wang, Dongming Xing, Wujun Chen
Summary: lncRNAs, as a unique group of RNA transcripts longer than 200 nucleotides, regulate the roles of ABCA1 and ABCG1 in cholesterol efflux, influencing the development of atherosclerosis.
CLINICA CHIMICA ACTA
(2021)
Review
Medical Laboratory Technology
Wujun Chen, Lu Li, Qian Li, Renshuai Zhang, Shuai Wang, Yudong Wu, Dongming Xing
Summary: Atherosclerosis is characterized by lipid accumulation and chronic inflammation, with YRNAs and YsRNAs playing important roles in the initiation, progression, and diagnosis of the disease, potentially serving as new therapeutic and diagnostic targets.
CLINICA CHIMICA ACTA
(2021)
Review
Medical Laboratory Technology
Wujun Chen, Lu Li, Jie Wang, Renshuai Zhang, Tingting Zhang, Yudong Wu, Shuai Wang, Dongming Xing
Summary: ABCA1 plays a central role in connecting cholesterol efflux and apoptotic cell clearance by regulating the release and expression of various ligands to promote efferocytosis.
CLINICA CHIMICA ACTA
(2021)
Review
Cell Biology
Xiao-Dan Xia, Adekunle Alabi, Maggie Wang, Hong-Mei Gu, Rui Zhe Yang, Gui-Qing Wang, Da-Wei Zhang
Summary: Lipids play essential roles in physiological functions, and dysregulation of lipid metabolism can lead to various human diseases. The regulation of lipid metabolism involves multiple mechanisms at different levels to maintain homeostasis.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2021)
Review
Cardiac & Cardiovascular Systems
Xiao-dan Xia, Zhong-sheng Peng, Hong-mei Gu, Maggie Wang, Gui-qing Wang, Da-wei Zhang
Summary: PCSK9 plays a vital role in promoting cardiovascular disease and cancer by degrading LDLR and affecting tumor growth. Inhibition of PCSK9 can reduce the risk of cardiovascular disease and cancer.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Xiaole Chang, Yongfang Zhao, Shucun Qin, Hao Wang, Bingxiang Wang, Lei Zhai, Boyan Liu, Hong-mei Gu, Da-wei Zhang
Summary: The adrenal gland in Surf4(LKO) mice shows reduced cholesterol levels and increased expression of genes involved in de novo cholesterol biosynthesis, leading to compensation for the loss of circulating lipoprotein-derived cholesterol and maintenance of adrenal steroid hormone production.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Medical Laboratory Technology
Miao Jiang, Xiao-Mei Zhao, Zhi-Sheng Jiang, Gui-Xue Wang, Da-wei Zhang
Summary: Accumulation of reactive oxygen species (ROS) can induce protein tyrosine nitration and endothelial dysfunction, playing an important role in atherosclerosis.
CLINICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Yishi Shen, Hong-mei Gu, Lei Zhai, Binxiang Wang, Shucun Qin, Da-wei Zhang
Summary: Elevated levels of LDL-C increase the risk of atherosclerotic cardiovascular disease. In this study, the role of Surf4 in lipoprotein metabolism and the development of atherosclerosis in apoE(-/-) mice was investigated. The results showed that knockdown of Surf4 expression significantly reduced triglyceride secretion and plasma levels of non-HDL cholesterol and triglycerides without causing hepatic lipid accumulation or liver damage. Knockdown of Surf4 also diminished atherosclerosis lesions. These findings suggest that hepatic Surf4 inhibition may be a novel therapeutic strategy to reduce the risk of atherosclerotic cardiovascular disease.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Review
Pharmacology & Pharmacy
Wujun Chen, Bing Liang, Xiaolin Wu, Ling Li, Chao Wang, Dongming Xing
Summary: On December 22, 2021, the United States FDA approved nirmatrelvir/ritonavir (Paxlovid), the first main protease inhibitor, for the treatment of early severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nirmatrelvir inhibits SARSCoV-2 infection, but high doses or long-term treatment may cause embryonic developmental toxicity and changes in host gene expression. Ritonavir enhances the efficacy of nirmatrelvir by inactivating cytochrome P450 3A4 expression and occupying plasma protein binding sites. Multidrug resistance protein 1 inhibitors may increase the effectiveness of nirmatrelvir.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Cardiac & Cardiovascular Systems
Maggie Wang, Adekunle Alabi, Hong-mei Gu, Govind Gill, Ziyang Zhang, Suha Jarad, Xiao-dan Xia, Yishi Shen, Gui-qing Wang, Da-wei Zhang
Summary: This study investigates the determinants of MT1-MMP-induced LDLR cleavage in LDLR and MT1-MMP. The study finds that MT1-MMP does not require a specific cleavage site on LDLR, but an amino acid residue with a hydrophobic side chain at position 167 in the MT-loop is critical for MT1-MMP-induced LDLR cleavage.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Cell Biology
Yishi Shen, Hong-Mei Gu, Shucun Qin, Da-Wei Zhang
Summary: Surfeit 4 is a transmembrane protein involved in cargo transport between the ER and Golgi apparatus. It plays a crucial role in lipid metabolism and has been implicated in various physiological and pathophysiological processes. However, the molecular mechanisms underlying its selective sorting of diverse cargos for protein trafficking are still unclear.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2023)
Article
Hematology
Geru Tao, Hao Wang, Yishi Shen, Lei Zhai, Boyan Liu, Bingxiang Wang, Wei Chen, Sijie Xing, Yuan Chen, Hong-Mei Gu, Shucun Qin, Da-Wei Zhang
Summary: In this study, the role of Surf4 in lipid absorption and chylomicron secretion was investigated. The findings suggest that inhibition of Surf4 can reduce serum lipid levels and decrease the risk of obesity and cardiovascular diseases. This study provides new insights for the therapeutic application of Surf4.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Review
Chemistry, Medicinal
Usman Sabir, Hong-mei Gu, Da-Wei Zhang
Summary: MMPs have a complex role in liver fibrosis, with high expression in the early stage causing tissue damage and promoting fibrosis progression, and downregulation in the later stage facilitating ECM accumulation and disease progression. Phytochemicals can modulate MMP activity and ECM turnover, alleviating disease progression, but their effects depend on disease models, stages, and the dosage, timing, and duration of phytochemicals used.
PHYTOTHERAPY RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Shouliang Wang, Haijiao Long, Lianjie Hou, Baorong Feng, Zihong Ma, Ying Wu, Yu Zeng, Jiahao Cai, Da-wei Zhang, Guojun Zhao
Summary: Mitochondria are dynamic organelles crucial for cell metabolism and survival, participating in necrotic cell death and programmed apoptotic. Mitophagy serves as a cytoprotective mechanism to maintain mitochondrial homeostasis and remove dysfunctional mitochondria. Understanding the molecular mechanisms and alterations in mitophagy is important for disease treatment and therapeutic target design.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)