Article
Biochemistry & Molecular Biology
Serena Mirra, Laura Sanchez-Bellver, Carmela Casale, Alessandra Pescatore, Gemma Marfany
Summary: Activation of NF-kappa B transcription factor is regulated in the retina to prevent excessive inflammation. Downregulation of USP48 stabilizes NF-kappa B/p65 and increases its transcriptional activity in retinal cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Natalia Petrenko, Kevin Struhl
Summary: Differences in the transcriptional initiation process exist among eukaryotic species, such as the dynamic bridge of Mediator and variations in TBP occupancy. TFIID interacts strongly at downstream regions of the initiation site in flies, contrasting with yeast and mammals. The Mediator subunit Med26 and Taf7 interact near the Pol II pause region, but only in subsets of genes.
Article
Oncology
Spyros Foutadakis, Eugenia Roupakia, Panagiotis Liakopoulos, Petros Kolovos, Evangelos Kolettas
Summary: Transcription Factors (TFs) are key regulators of gene expression, controlling aspects such as cell homeostasis, identity, and fate. NF-kappa B and E2F1 are important TFs involved in inflammation and cell cycle progression, respectively. This study compared their genomic binding profiles and found distinct preferences in the type of DNA elements they bind to. Additionally, NF-kappa B and E2F1 showed limited overlap in their binding sites and tended to bind to active chromatin even before stimulation. Furthermore, NF-kappa B was found to recruit E2F1 near pro-inflammatory genes following immune stimulation.
Article
Cell Biology
Inna Rabinovich-Nikitin, Alexandra Blant, Rimpy Dhingra, Lorrie A. Kirshenbaum, Michael P. Czubryt
Summary: Hypoxia has broad effects on cardiomyocyte function and viability, and this study reveals that the transcriptional coactivator PGC-1 alpha is down-regulated in hypoxia through the nuclear localization of NF-kappa B p65 subunit and its association with the PGC-1 alpha promoter.
Article
Pharmacology & Pharmacy
Bin Yang, Huijun Yang, Xiaomei Lu, Laiyuan Wang, Hongfan Li, Shufeng Chen, Xianqiang Wang, Chenyang Shen, Jianfeng Huang, Xiangfeng Lu, Dongfeng Gu
Summary: The study demonstrated that miR-520b is expressed in human atherosclerotic plaques and suppresses EC inflammation by down-regulating the NF-KB p65-ICAM1/VCAM1 axis, regulating the interaction between monocytes and ECs, and potentially serving as a therapeutic target for EC dysfunction and atherosclerosis.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Immunology
Ye Cui, Mehdi Benamar, Klaus Schmitz-Abe, Varsha Poondi-Krishnan, Qian Chen, Bat-Erdene Jugder, Benoit Fatou, Jason Fong, Yuelin Zhong, Stuti Mehta, Altantsetseg Buyanbat, Beray Selver Eklioglu, Esra Karabiber, Safa Baris, Ayca Kiykim, Sevgi Keles, Emmanuel Stephen-Victor, Claudia Angelini, Louis-Marie Charbonnier, Talal A. Chatila
Summary: This study identifies Stk4 as a crucial regulator in regulatory T cells, which promotes immune tolerance by regulating the transcriptional programs of Foxp3 and p65 through complex formation.
SCIENCE IMMUNOLOGY
(2022)
Article
Chemistry, Medicinal
Yishan Zhou, Bingling Zhong, Xiangjing Min, Ying Hou, Ligen Lin, Qin Wu, Jingshan Shi, Xiuping Chen
Summary: This study demonstrated that isobavachalcone (IBC) could ameliorate colitis in mice possibly through inhibition of NF-kappa B p65.
PHYTOTHERAPY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Marina Kolesnichenko, Nadine Mikuda, Uta E. Hoepken, Eva Kaergel, Bora Uyar, Ahmet Bugra Tufan, Maja Milanovic, Wei Sun, Inge Krahn, Kolja Schleich, Linda Hoff, Michael Hinz, Michael Willenbrock, Sabine Jungmann, Altuna Akalin, Soyoung Lee, Ruth Schmidt-Ullrich, Clemens A. Schmitt, Claus Scheidereit
Summary: The study reveals that DNA double-strand breaks elicit two subsequent phases of NF-kappa B activation, with the first phase controlling anti-apoptotic gene expression and the second phase driving expression of senescence-associated genes.
Article
Virology
Salma Aktar, Jun Arii, Lidya Handayani Tjan, Mitsuhiro Nishimura, Yasuko Mori
Summary: The study reveals that HHV-6A infection activates the NF-KB pathway through the U14 protein, leading to upregulation of proinflammatory cytokines and promoting viral replication. Inhibition of the NF-KB pathway affects HHV-6A replication, and the results indicate the functional role of HHV-6A U14 in host cell signaling, providing new insights into the pathogenesis and replication mechanisms of HHV-6A.
JOURNAL OF VIROLOGY
(2021)
Article
Integrative & Complementary Medicine
Yishan Zhou, Shuangli Xiang, Haoyi Zheng, Ying Hou, Ying Wang, Chuang-Chuang Li, Qin Wu, Jingshan Shi, Xiuping Chen
Summary: The inhibitory effect of neferine on colitis-associated colorectal cancer was investigated, revealing its potential role in regulating NF-kappa B and STAT3 signaling pathways.
AMERICAN JOURNAL OF CHINESE MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Yu-fei Wang, Jin-yan Feng, Li-na Zhao, Man Zhao, Xian-fu Wei, Yu Geng, Hong-feng Yuan, Chun-yu Hou, Hui-hui Zhang, Guo-wen Wang, Guang Yang, Xiao-dong Zhang
Summary: Aspirin induces ferroptosis in liver cancer cells by inhibiting the transcription of SLC7A11 gene activated by NF-κB-p65. This finding provides a new strategy for the combination therapy of HCC using aspirin and ferroptosis inducer.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Immunology
Xin Lin, Kenji Tago, Nozomi Okazaki, Takanori So, Kyoko Takahashi, Tadahiko Mashino, Hiroomi Tamura, Megumi Funakoshi-Tago
Summary: In this study, the newly synthesized indole-hydantoin derivative IH-1 showed significant inhibitory effects on LPS-induced inflammatory responses in murine macrophage-like cells, indicating its potential as an anti-inflammatory drug.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Immunology
Masashi Mizuno, Rei Nakano, Saki Nose, Moeka Matsumura, Yasuyuki Nii, Kentaro Kurogochi, Hiroshi Sugiya, Masami Uechi
Summary: In this study, it was found that interleukin 1 beta (IL-1 beta) induces the expression of interleukin 8 (IL-8) in canine cardiac fibroblasts through the activation of nuclear factor (NF)-kappa B p65.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Bin Lin, Hui Zhao, Li Li, Zhenzhen Zhang, Nan Jiang, Xiaowei Yang, Tao Zhang, Bowen Lian, Yaokai Liu, Chi Zhang, Jiaxiang Wang, Feng Wang, Deguang Feng, Jing Xu
Summary: The findings suggest that Sirt1 plays a role in alleviating heart failure in rats through the NF-kappa B p65/miR-155/BDNF signaling cascade. Inhibition of Sirt1 increased acetylation of NF-kappa B p65, leading to elevated NF-KB p65 expression, while silencing NF-kappa B p65 improved heart functions and reduced cardiomyocyte apoptosis.
Article
Biochemistry & Molecular Biology
Jiayin Lu, Yaoxing Chen, Zixu Wang, Jing Cao, Yulan Dong
Summary: Restraint stress during pregnancy leads to increased levels of corticosterone, norepinephrine, and blood glucose, while decreasing estrogen levels. This stress also activates the beta(2)-AR/FOXO1/p-NF-kappa B pathway, resulting in increased oxidative stress in the maternal uterus. This study sheds light on the impact of psychological stress on pregnant women and their reproductive health.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Oncology
Selena Mimmi, Domenico Maisano, Nancy Nistico, Eleonora Vecchio, Federico Chiurazzi, Katia Ferrara, Marialuigia Iannalfo, Alessandro D'Ambrosio, Giuseppe Fiume, Enrico Iaccino, Ileana Quinto
Article
Public, Environmental & Occupational Health
Giuseppe Limongelli, Stefano Iucolano, Emanuele Monda, Pasquale Elefante, Chiara De Stasio, Imma Lubrano, Martina Caiazza, Marialuisa Mazzella, Fabio Fimiani, Maria Galdo, Giulia De Marchi, Martina Esposito, Marta Rubino, Annapaola Cirillo, Adelaide Fusco, Augusto Esposito, Ugo Trama, Salvatore Esposito, Gioacchino Scarano, Joseph Sepe, Generoso Andria, Valentina Orlando, Enrica Menditto, Paolo Chiodini
Summary: This study investigates the capacity of the rare disease healthcare network in Campania to diagnose patients with rare diseases during the Covid-19 outbreak, and sheds light on problematic diagnoses during this period. The results show that the prevalence of new diagnoses of rare disease was significantly lower during the outbreak, and various issues such as lack of diagnostic resources and guidelines were identified.
JOURNAL OF PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Eleonora Vecchio, Nancy Nistico, Gaetanina Golino, Enrico Iaccino, Domenico Maisano, Selena Mimmi, Annamaria Aloisio, Maurizio Renna, Angelica Avagliano, Alessandro Arcucci, Giuseppe Fiume, Ileana Quinto
Summary: The IBTK gene encodes the IBtk alpha protein, which plays a role in the regulation of MYC-dependent B-lymphomagenesis. Mechanistically, IBtk alpha indirectly activates the beta-catenin-dependent transcription of the MYC gene by modulating GSK3 beta. Silencing IBtk alpha leads to downregulation of MYC expression and increased apoptosis in Burkitt's lymphoma cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Selena Mimmi, Domenico Maisano, Vincenzo Dattilo, Massimo Gentile, Federico Chiurazzi, Alessandro D'Ambrosio, Annamaria Zimbo, Nancy Nistico, Annamaria Aloisio, Eleonora Vecchio, Giuseppe Fiume, Enrico Iaccino, Ileana Quinto
Summary: Chronic Lymphocytic Leukemia (CLL) is a heterogeneous disease with variable clinical courses among different patients. This study discovered the possible existence of multiple independent CLL clones within the same patient. By selecting a peptide binder that binds to specific clone cells using phage display libraries and performing flow cytometry, it was found that the specific clone cells expressed more genes involved in survival and apoptosis escape processes compared to other clone cells, and they did not carry characteristic genetic lesions.
Article
Biochemistry & Molecular Biology
Veronica Romano, Maria Rosaria Ruocco, Pietro Carotenuto, Anna Barbato, Alessandro Venuta, Vittoria Acampora, Sabrina De Lella, Elena Vigliar, Antonino Iaccarino, Giancarlo Troncone, Gaetano Cali, Luigi Insabato, Daniela Russo, Brunella Franco, Stefania Masone, Nunzio Velotti, Antonello Accurso, Tommaso Pellegrino, Giuseppe Fiume, Immacolata Belviso, Stefania Montagnani, Angelica Avagliano, Alessandro Arcucci
Summary: Breast cancer-associated fibroblasts (BCAFs) promote breast cancer progression by interacting with cancer cells. In an in vitro experiment, BCAFs became inactivated after 216 hours of 3D culture, resulting in a cytostatic effect on MCF-7 cells. Reverted BCAFs also exhibited an inactivated phenotype and inhibited the migration of MCF-7 cells. These findings suggest that deactivation of BCAFs without drug treatment reduces their ability to sustain breast cancer progression in vitro.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Salvatore Rotundo, Eleonora Vecchio, Antonio Abatino, Caterina Giordano, Serafina Mancuso, Maria Teresa Tassone, Chiara Costa, Alessandro Russo, Enrico Maria Trecarichi, Giovanni Cuda, Francesco Saverio Costanzo, Camillo Palmieri, Carlo Torti
Summary: This study found that short-term memory T-cell responses were not significantly hindered in COVID-19 patients after receiving neutralizing monoclonal antibody treatment. Different moAbs may have diverse impacts on the evolution of S-specific T-cell responses in vaccinated and unvaccinated patients.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Maddalena Di Sanzo, Flora Cozzolino, Anna Martina Battaglia, Ilenia Aversa, Vittoria Monaco, Alessandro Sacco, Flavia Biamonte, Camillo Palmieri, Francesca Procopio, Gianluca Santamaria, Francesco Ortuso, Piero Pucci, Maria Monti, Maria Concetta Faniello
Summary: The study found that the interaction between FTH1 and PRDX6 in cancer cells can affect cell proliferation and migration, leading to a less invasive phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Angelica Avagliano, Giuseppe Fiume, Claudio Bellevicine, Giancarlo Troncone, Alessandro Venuta, Vittoria Acampora, Sabrina De Lella, Maria Rosaria Ruocco, Stefania Masone, Nunzio Velotti, Pietro Carotenuto, Massimo Mallardo, Carmen Caiazza, Stefania Montagnani, Alessandro Arcucci
Summary: This article reviews the role of cancer-associated fibroblasts (CAFs) in thyroid cancer, highlighting the importance of studying CAFs' contribution to understanding the mechanisms of cancer growth and developing new therapeutic strategies.
Article
Biochemistry & Molecular Biology
Carmen Caiazza, Teresa Brusco, Federica D'Alessio, Massimo D'Agostino, Angelica Avagliano, Alessandro Arcucci, Concetta Ambrosino, Giuseppe Fiume, Massimo Mallardo
Summary: This study demonstrates the important role of STING in antigen presentation. The absence of STING leads to impairment of peptide presentation and dampened immune response activation. This has significant implications for understanding the role of STING in immune mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Selena Mimmi, Nicola Lombardo, Domenico Maisano, Giovanna Piazzetta, Corrado Pelaia, Girolamo Pelaia, Marta Greco, Daniela Foti, Vincenzo Dattilo, Enrico Iaccino
Summary: Dupilumab has been proven effective in treating diseases such as asthma and atopic dermatitis, and now its use as add-on therapy for patients with CRSwNP could lead to reduced remission time and improved quality of life. The study also showed that miRNAs may serve as potential biomarkers for immune modulation. Preliminary data indicated that dupilumab treatment led to a reduction in inflammation and modulation of specific miRNAs related to proliferation, inflammation, and drug-resistance.
Article
Multidisciplinary Sciences
Eugenio Garofalo, Flavia Biamonte, Camillo Palmieri, Anna Martina Battaglia, Alessandro Sacco, Eugenio Biamonte, Giuseppe Neri, Giulio Cesare Antico, Serafina Mancuso, Giuseppe Foti, Carlo Torti, Francesco Saverio Costanzo, Federico Longhini, Andrea Bruni
Summary: In this study, we evaluated the spike-specific immune response in patients with breakthrough SARS-CoV-2 infection after receiving two doses of the BNT162b2 mRNA vaccine. The results showed that severe COVID-19 patients requiring hospitalization had lower levels of spike-specific IFN gamma-releasing cells compared to mild-moderate patients and healthy subjects, despite similar antibody titers.
Article
Cell Biology
Nancy Nistico, Annamaria Aloisio, Antonio Lupia, Anna Maria Zimbo, Selena Mimmi, Domenico Maisano, Rossella Russo, Fabiola Marino, Mariangela Scalise, Emanuela Chiarella, Teresa Mancuso, Giuseppe Fiume, Daniela Omodei, Antonella Zannetti, Giuliana Salvatore, Ileana Quinto, Enrico Iaccino
Summary: In this study, we used phage display to select two highly specific peptide ligands for targeting the overexpressed EGFR in TNBC cells. Molecular docking predicted the peptides' binding affinities and sites on the extracellular domain of EGFR. Flow cytometry validated the binding of FITC-conjugated peptides to TNBC cells. Confocal microscopy confirmed the peptide binding specificity to EGFR-positive tumor tissues. These peptides could be used in conjunction with nanoparticles for tumor-targeted delivery of anticancer drugs.
Article
Pediatrics
Bruno Corrado, Nadia Sommella, Gianluca Ciardi, Enza Raiano, Iris Scala, Pietro Strisciuglio, Clemente Servodio Iammarrone
Summary: The study revealed that the average age for children with Down syndrome to start walking is 26 months. Factors such as being male, having trisomy 21, and improved joint ligamentous laxity were found to negatively impact the onset of walking, which could be offset by early physical therapy.
MINERVA PEDIATRICS
(2022)