Article
Reproductive Biology
Rong Li, Tian-yuan Wang, Xin Xu, Olivia M. Emery, MyeongJin Yi, San-Pin Wu, Francesco J. DeMayo
Summary: By using spatial transcriptomics and single-cell RNA-seq assays, we identified the gene expression profiles at the embryo implantation site in the mouse uterus on pregnancy day 7.5. Our results revealed 11 distinct gene signature domains and 12 types of cells. Functional annotations showed that uterine microenvironments have region-specific metabolic preferences, immune responses, and cellular behaviors. This study provides high-resolution transcriptome profiles for further investigations on molecular mechanisms of normal pregnancy progression.
BIOLOGY OF REPRODUCTION
(2022)
Article
Multidisciplinary Sciences
Katrin Mangold, Jingyan He, Sanne Stokman, Emma R. Andersson
Summary: Manipulating gene expression in the developing mouse brain in utero offers great potential for functional genetics studies. The protocol described in this article allows for successful transduction in more than 95% of the developing brain, using high-titer lentivirus. Additionally, injection at embryonic day 7.5 can target ectoderm and neural crest contributing to various tissues and organs.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Multidisciplinary Sciences
Jinfeng Jiang, Tong Qiu, Chao Yang, Yuan Yuan, Ling Qin, Peixuan Zhang
Summary: The short G1 phase of embryonic stem cells results from the promotion of G1/S transition by MEK1/2 kinases, but it is not necessarily related to pluripotency maintenance. Inhibition of MEK1/2 kinases in naive ESCs leads to a longer G1 phase. This inhibition increases the level of reactive oxygen species (ROS) and stabilizes the p53 protein. The genetic ablation of p53 transforms the cell cycle profile of naive ESCs to that of primed ESCs.
Article
Cell Biology
Sena Aksel, Mei Cao, Amber Derpinghaus, Laurence S. Baskin, Gerald R. Cunha
Summary: In this study, the researchers used immunohistochemistry to examine the temporal-spatial expression of different somatic cell types in the mouse testis. They found inconsistencies in the expression patterns compared to previous studies and raised questions about the conservation of developmental mechanisms in higher vertebrates.
Article
Cell Biology
Po-Yu Lin, Denny Yang, Chi-Hsuan Chuang, Hsuan Lin, Wei-Ju Chen, Chia-Ying Chen, Trees-Juen Chuang, Chien-Ying Lai, Long-Yuan Li, Scott C. Schuyler, Frank Leigh Lu, Yu-Chuan Liu, Jean Lu
Summary: The study introduces a new experimental model, 'cluster 3', as a subpopulation of TBLCs that can be molecularly defined as near totipotent cells.
Article
Multidisciplinary Sciences
Daisuke Arai, Yoichi Nakao
Summary: In this study, an optimized HDR-mediated knock-in method for mouse embryonic stem cells was developed, which significantly improved efficiency and minimized random integrations caused by NHEJ/MMEJ. The BiPoD method achieved efficient plasmid donor insertion and increased biallelic knock-in efficiency, providing a rapid and easy way to establish biallelic knock-in mESC lines.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Maria Mougkogianni, Argyro Kalogeropoulou, Nickolaos Nikiforos Giakoumakis, Zoi Lygerou, Stavros Taraviras
Summary: Recruitment of the MCM complex on DNA replication origins is critical for faithful genome duplication, with aberrant licensing linked to cancer and neurodevelopmental diseases. An optimized approach for qualitative and quantitative analysis of DNA-bound MCMs in the developing mouse cortex has been developed, offering innovative insights into in vivo research on origin licensing.
Article
Biochemical Research Methods
Stylianos Kosmidis, Adrian Negrean, Alex Dranovsky, Attila Losonczy, Eric R. Kandel
Summary: Fast 3D Clear is an aqueous clearing protocol that can achieve high transparency of adult and embryonic mouse tissues in a few days, making it suitable for a wide range of biomedical studies.
CELL REPORTS METHODS
(2021)
Article
Cell Biology
Fatemeh Choupani, Vahideh Assadollahi, Zakaria Vahabzadeh, Erfan Daneshi, Morteza Abouzaripour, Farzad Soleimani, Saman Bahrami, Fardin Fathi
Summary: The study compared the expression levels of Fgf2, Bmp4, ActivinA, Lif, and Tgfb1 genes in MEFs at different passages isolated from 13.5-day-old and 15.5-day-old embryos of NMRI and C57BL/6 mice. Differences were observed in gene expression levels and growth factor secretion between different passages, genetic backgrounds, and ages of mouse embryos.
Article
Biology
Kaustav Mukherjee, James J. Bieker
Summary: The paper presents a novel method for isolating F4/80+ cells from E13.5 mouse fetal liver using magnetic nanoparticles and disrupting erythroid macrophage interactions with a peptide, resulting in healthy F4/80+ single cells free of erythroid contamination. The protocol generates a population enriched in F4/80+ cells suitable for sensitive techniques like single cell sequencing.
Article
Neurosciences
Lesley A. Colgan, Mo Hu, Jaime A. Misler, Paula Parra-Bueno, Corey M. Moran, Michael Leitges, Ryohei Yasuda
NATURE NEUROSCIENCE
(2018)
Article
Geriatrics & Gerontology
Mini P. Sajan, Barbara C. Hansen, Margaret G. Higgs, C. Ron Kahn, Ursula Braun, Michael Leitges, Collin R. Park, David M. Diamond, Robert V. Farese
NEUROBIOLOGY OF AGING
(2018)
Article
Biochemistry & Molecular Biology
Susanne Vorhagen, Dominik Kleefisch, Oana-Diana Persa, Annika Graband, Alexandra Schwickert, Michael Saynisch, Michael Leitges, Carien M. Niessen, Sandra Iden
Retraction
Biology
Diasynou Fioravante, YunXiang Chu, Arthur P. H. de Jong, Michael Leitges, Pascal S. Kaeser, Wade G. Regehr
Article
Cell Biology
Kerstin Siegmund, Nikolaus Thuille, Nina Posch, Friedrich Fresser, Michael Leitges, Gottfried Baier
CELL COMMUNICATION AND SIGNALING
(2019)
Article
Oncology
Ning Yin, Yi Liu, Andras Khoor, Xue Wang, E. Aubrey Thompson, Michael Leitges, Verline Justilien, Capella Weems, Nicole R. Murray, Alan P. Fields
Article
Biochemistry & Molecular Biology
Alexander E. Mayer, Mona C. Loeffler, Angel E. Loza Valdes, Werner Schmitz, Rabih El-Merahbi, Jonathan Trujillo Viera, Manuela Erk, Thianzhou Zhang, Ursula Braun, Mathias Heikenwalder, Michael Leitges, Almut Schulze, Grzegorz Sumara
Article
Cell Biology
Nikolaus Thuille, Kerstin Siegmund, Victoria Klepsch, Jacqueline Schoergenhuber, Sarah Danklmaier, Michael Leitges, Gottfried Baier
CELL COMMUNICATION AND SIGNALING
(2019)
Correction
Oncology
Ning Yin, Yi Liu, Andras Khoor, Xue Wang, E. Aubrey Thompson, Michael Leitges, Verline Justilien, Capella Weems, Nicole R. Murray, Alan P. Fields
Meeting Abstract
Endocrinology & Metabolism
Mini P. Sajan, Ursula Braun, Michael Leitges, Colin Park, David M. Diamond, Jin Wu, Barbara C. Hansen, Mildred Acevedo Duncan, Christopher A. Apostolatos, Andre H. Apostolatos, Mark Kindy, Robert V. Farese
METABOLISM-CLINICAL AND EXPERIMENTAL
(2020)
Article
Cell Biology
Eugene Park, Jingyu Chen, Andrew Moore, Maurizio Mangolini, Antonella Santoro, Joseph R. Boyd, Hilde Schjerven, Veronika Ecker, Maike Buchner, James C. Williamson, Paul J. Lehner, Luca Gasparoli, Owen Williams, Johannes Bloehdorn, Stephan Stilgenbauer, Michael Leitges, Alexander Egle, Marc Schmidt-Supprian, Seth Frietze, Ingo Ringshausen
SCIENCE TRANSLATIONAL MEDICINE
(2020)
Article
Multidisciplinary Sciences
Alicia K. Fleming Martinez, Heike R. Doppler, Ligia Bastea, Brandy Edenfield, Tushar Patel, Michael Leitges, Geou-Yarh Liou, Peter Storz
Summary: DCLK1(+) pancreatic cancer stem cells develop at a precancerous stage and may contribute to the lack of efficacy of pancreatic cancer therapy. Our study showed that EGFR signaling is not propagated to the nucleus in DCLK1(+) PanIN cells, and inhibition of EGFR led to a significant increase in DCLK1(+) PanIN cells. The activation of PKD1 by an increase in hydrogen peroxide is identified as a mechanism for the formation of DCLK1(+) cells, driving stemness and abundance in lesions.
Article
Oncology
Kristin S. Inman, Yi Liu, Michele L. Scotti Buzhardt, Michael Leitges, Murli Krishna, Howard C. Crawford, Alan P. Fields, Nicole R. Murray
Summary: The protein kinase C iota (PKC iota) plays a promotive role in pancreatic cancer development, and inhibition of its expression prevents pancreatic cancer formation. It also affects the autophagy of pancreatic epithelial cells and the progression of pancreatic cancer.
Article
Cell & Tissue Engineering
Franziska von Heydebrand, Maximilian Fuchs, Meik Kunz, Simon Voelkl, Anita N. Kremer, Robert A. J. Oostendorp, Jochen Wilke, Michael Leitges, Alexander Egle, Andreas Mackensen, Gloria Lutzny-Geier
Summary: PKC beta plays a significant role in influencing glucose metabolism in CLL cells upon contact with BMSC, by stimulating glucose uptake to alleviate stress and apoptosis in CLL cells, and could potentially be targeted as a therapeutic strategy to overcome drug resistance mediated by the stromal microenvironment.
Meeting Abstract
Hematology
Eugene Park, Jingyu Chen, Andrew Moore, Michael Leitges, Seth E. Frietze, Ingo Ringshausen