Article
Multidisciplinary Sciences
Theodore A. Chavkin, Loc-Duyen Pham, Aleksandar Kostic
Summary: The study found that oral probiotics EcN can reduce postprandial glycemic response in mice, but not through direct glucose uptake. Further exploration is needed to understand the potential indirect mechanisms of EcN on host glucose metabolism.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
Haojie Chen, Pengyu Lei, Hao Ji, Jiahui Ma, Yimeng Fang, Haiyang Yu, Jiao Du, Linkai Qu, Qinsi Yang, Li Luo, Kun Zhang, Wei Wu, Libo Jin, Da Sun
Summary: This study evaluated the safety and efficacy of Escherichia coli Nissle 1917 (EcN) ghosts as drug carriers for treating inflammatory bowel disease (IBD). The experiments showed that EcN ghosts had high adhesion and could attenuate IBD symptoms by inhibiting neutrophil chemotaxis and reactive oxygen species production. Due to the unique biological functions of EcN ghosts, they may be used as a strategy for targeted drug delivery in future IBD treatment.
Article
Biotechnology & Applied Microbiology
Dandan Wu, Xiaomei Li, Yanying Yu, Bingxue Gong, Xianxuan Zhou
Summary: The study showed that heparin stimulated the biofilm formation of EcN. Both the sulfation and chain length of heparin contributed to enhancing the biofilm formation of EcN, which could potentially promote the intestinal colonization of probiotics to combat pathogen infections.
BIOTECHNOLOGY LETTERS
(2021)
Article
Microbiology
Guixia Yang, Lingkang Yang, Xianxuan Zhou
Summary: In this study, the researchers identified the flagellin protein FliC of Escherichia coli Nissle1917 (EcN) as a heparin binding protein. They found that heparin's ability to bind FliC was 48 times higher than its ability to bind other proteins. The results demonstrate that interaction between heparin and EcN's flagellin FliC inhibits bacterial swimming.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Miao-Miao Dong, Lu Song, Jia-Qi Xu, Lin Zhu, Liang-Bin Xiong, Dong-Zhi Wei, Feng-Qing Wang
Summary: This study presents a simple design to minimize genetic changes in probiotics by eliminating native plasmids and reintroducing recombinants carrying functional genes. Specific insertion sites in the vectors showed significant differences in fluorescence protein expression. This design successfully achieved the synthesis of salicylic acid and ergothioneine through de novo synthesis and one-step construction, respectively. It expands the application scope of native cryptic plasmids for easy construction of functional pathways.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yi Ma, Wei Fu, Bin Hong, Xinfeng Wang, Shoujin Jiang, Jufang Wang
Summary: In this study, it was found that Probiotic Escherichia coli Nissle 1917 (EcN) inhibits the growth of Salmonella by producing the antimicrobial substance MccM. EcN::mcmA strain also reduced the adhesion and invasion ability of Salmonella to intestinal epithelial cells and decreased the secretion of inflammatory factors. These findings suggest that EcN::mcmA can be developed as engineered probiotics to combat pathogenic enterobacteria colonization in the gut.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Courtney Jones, Brianna G. Jarboe, Haley M. Majer, Amy T. Ma, Joris Beld
Summary: Escherichia coli Nissle 1917 is a Gram-negative bacterium used for treating inflammatory bowel diseases, with its ability to produce secondary metabolites playing a key role in its activity. The APE biosynthetic gene cluster and the interaction between acyl carrier proteins and phosphopantetheinyl transferase (PPTase) in EcN are essential for understanding its function. This knowledge is crucial for engineering secondary metabolite production hosts and targeting PPTases with new antibiotics.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Rui Zhao, Zimai Li, Yuqing Sun, Wei Ge, Mingyu Wang, Huaiwei Liu, Luying Xun, Yongzhen Xia
Summary: Hyperuricemia, the second most prevalent metabolic disease, can be effectively treated by introducing recombinant urate-degrading bacteria to enhance intestinal secretion, according to a study.
Review
Microbiology
Sefli Sri Wahyu Effendi, I-Son Ng
Summary: EcN is a notable probiotic used to treat Crohn's disease and irritable bowel syndrome. A comprehensive genomic database is helpful in assessing EcN's role in human health. Recent advancements in synthetic and genetic tools have made it possible to engineer EcN as a living medicine with controllable functions. Further studies are needed to fully explore the potential of EcN in disease treatment.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Microbiology
Ahmad M. Aljohani, Cecile El-Chami, Muna Alhubail, Ruth G. Ledder, Catherine A. O'Neill, Andrew J. McBain
Summary: In this study, cell-free supernatants (CFS) from 25 human commensal and associated bacteria were tested for their activity against Pseudomonas aeruginosa. Among them, Escherichia coli Nissle 1917 CFS was found to inhibit biofilm formation and disperse existing Pseudomonas biofilms without affecting planktonic bacterial growth. The CFS also downregulated the expression of Pseudomonas aeruginosa proteins involved in motility and quorum sensing, which are associated with biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Engineering, Biomedical
Danjun Wu, Zejing Zhao, Hong Liu, Kaili Fu, Yaning Ji, Weili Ji, Yazhen Li, Qinying Yan, Gensheng Yang
Summary: By utilizing bacteria-driven microrobots, therapeutic drugs and photosensitizers can be delivered together to the deep tumor site, achieving a self-propelled strategy that enhances drug penetration and improves chemosensitivity, resulting in a superior antitumor effect.
ACTA BIOMATERIALIA
(2023)
Review
Biotechnology & Applied Microbiology
Mingjing Yu, Shilong Hu, Biao Tang, Hua Yang, Dongchang Sun
Summary: Genetically engineered microbes, especially Escherichia coli, have been widely used in the biosynthesis of proteins and metabolites for medical and industrial applications. E. coli Nissle 1917 (EcN) has recently emerged as a microbial chassis for generating living therapeutics, drug delivery vehicles, and microbial platforms for industrial production. New genetic engineering systems are still greatly needed to expand the application range of EcN, despite the availability of genetic tools for engineering laboratory E. coli K-12 and B strains.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Microbiology
Jean-Philippe Nougayrede, Camille Chagneau, Jean-Paul Motta, Nadege Bossuet-Greif, Marcy Belloy, Frederic Taieb, Jean Jacques Gratadoux, Muriel Thomas, Philippe Langella, Eric Oswald
Summary: The probiotic Escherichia coli strain Nissle 1917, believed to be beneficial and safe, actually harbors a pathogenic island that codes for a genotoxin linked to potential DNA damage and colorectal cancer. Using this probiotic may pose long-term adverse effects and should be carefully evaluated for risk versus benefit.
Article
Biophysics
Ting Wang, Qiong Yin, Hao yang Huang, Zhenyu Wang, Haixing Song, Xiaoming Luo
Summary: The poor drug penetration in the hypoxia area of solid tumors poses a significant challenge for intestinal tumor therapy. In this study, the researchers developed a bacteria propelled micro-robot using Escherichia coli Nissle 1917 (EcN) bacteria to target and deliver drugs to the hypoxic region of intestinal tumors.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Microbiology
Yasmine Rebai, Lysett Wagner, Mayssa Gnaien, Merle L. Hammer, Mario Kapitan, Maria Joanna Niemiec, Wael Mami, Amor Mosbah, Erij Messadi, Helmi Mardassi, Slavena Vylkova, Ilse D. Jacobsen, Sadri Znaidi
Summary: In this study, it was found that the probiotic strain Escherichia coli Nissle 1917 (EcN) can inhibit the growth of C. albicans and prevent its damage to intestinal cells. Through co-culture experiments and cell culture models, it was demonstrated that EcN can reduce the abundance of C. albicans and protect enterocytes from damage. This suggests that EcN may be a potential therapeutic approach against C. albicans infections.
Article
Biology
Krishnendu Mukherjee, Ulrich Dobrindt
Summary: This article reviews the contributions of chromatin remodeling to insect immunity and adaptation to pathogens, focusing on the roles of DNA methylation, histone acetylation, and microRNAs in the evolution of insect resistance.
CURRENT OPINION IN INSECT SCIENCE
(2022)
Article
Evolutionary Biology
Nicolas C. H. Schroeder, Ana Korsa, Haleluya Wami, Olena Mantel, Ulrich Dobrindt, Joachim Kurtz
Summary: This study investigated the persistence and evolution of the probiotic Escherichia coli strain Nissle 1917 in an insect host and flour environment through experimental evolution. The findings suggest that EcN remains stable with weak phenotypic changes during serial passage in the insect host, indicating a certain degree of phenotypic plasticity.
EVOLUTION MEDICINE AND PUBLIC HEALTH
(2022)
Article
Cell Biology
Laure David, Frederic Taieb, Marie Penary, Pierre-Jean Bordignon, Remi Planes, Salimata Bagayoko, Valerie Duplan-Eche, Etienne Meunier, Eric Oswald
Summary: This study revealed that OMVs carried by Escherichia coli inhibit autophagic flux and activate the non-canonical inflammasome pathway, exacerbating pathogenic properties of the infection.
Article
Biochemistry & Molecular Biology
Thibaut Molinie, Elodie Cougouilles, Claudine David, Edern Cahoreau, Jean-Charles Portais, Arnaud Mourier
Summary: The mitochondrial respiratory chain plays a crucial role in metabolic processes by regenerating NAD+ and ATP. Recent studies have shown that complex I can form functional entities called respirasomes, which facilitate NADH oxidation. However, findings demonstrate that complex II is preferred for electron transfer in heart and liver mitochondria. This unexpected result suggests a new regulatory mechanism for mitochondrial respiration in response to NADH/NAD+ redox stress.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Toxicology
Robert Landsiedel, Daniela Hahn, Rainer Ossig, Sabrina Ritz, Lydia Sauer, Roland Buesen, Sascha Rehm, Wendel Wohlleben, Sibylle Groeters, Volker Strauss, Saskia Sperber, Haleluya Wami, Ulrich Dobrindt, Karola Prior, Dag Harmsen, Bennard van Ravenzwaay, Juergen Schnekenburger
Summary: The analysis of intestinal microbiome and plasma metabolome can serve as an early and sensitive indicator of gut microbiome changes induced by orally administered nanoparticles; this will help to recognize potential adverse effects of these changes to the host.
PARTICLE AND FIBRE TOXICOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Camille V. Chagneau, Delphine Payros, Min Tang-Fichaux, Frederic Auvray, Jean-Philippe Nougayrede, Eric Oswald
Summary: In this review, the available literature is summarized to address questions regarding the function and presence of the biosynthetic gene cluster coding for colibactin, as well as its distribution in commensal and probiotic strains of Escherichia coli and other bacterial species.
TRENDS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Maryse Michele Um, Veronique Dupouy, Nathalie Arpaillange, Clemence Bieche-Terrier, Frederic Auvray, Eric Oswald, Hubert Brugere, Delphine Bibbal
Summary: The aim of this study was to determine the prevalence of mcr-positive E. coli strains in veal calves at the time of slaughter in France. Results showed that 16.5% of the tested calves carried mcr-positive E. coli, with some calves carrying multiple non-redundant strains. The majority of the mcr-positive isolates were multidrug-resistant, including resistance to ciprofloxacin, and carried bla(CTX-M) genes. The study also found the co-occurrence of mcr-3 genes with bla(CTX-M-55) genes.
Article
Multidisciplinary Sciences
Marie Corteggiani, Nadege Bossuet-Greif, Jean-Philippe Nougayrede, Deborah Byrne, Marianne Ilbert, Sebastien Dementin, Marie-Therese Giudici-Orticoni, Vincent Mejean, Eric Oswald, Olivier Genest
Summary: Chaperone proteins, such as Hsp90, play a critical role in maintaining protein homeostasis in all living cells. Recent studies have shown that bacterial Hsp90 is essential for the survival of bacteria under stress conditions and is involved in the virulence of pathogenic bacteria. In this study, the researchers used site-directed mutagenesis to disrupt the binding between Hsp90 and the chaperone protein DnaK, and found that this collaboration is crucial for the folding of client proteins in bacteria. They also demonstrated that this collaboration between Hsp90 and DnaK in bacteria has evolved to form a more complex collaboration between Hsp70 and Hsp90 in eukaryotes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Paulo A. Schueroff, Cecilia M. Abe, Jonatas W. Silva, Cideli de Paula Coelho, Fernanda B. Andrade, Rodrigo T. Hernandes, Ulrich Dobrindt, Tania A. T. Gomes, Waldir P. Elias
Summary: This study evaluated the involvement of AFP and other adhesins in the uropathogenicity and intestinal colonization of bacteria. The results showed a synergistic role of AFP and TIF in the adherence and colonization of intestinal and urinary epithelia.
Article
Microbiology
Lang Peng, Rexford Mawunyo Dumevi, Marco Chitto, Nadja Haarmann, Petya Berger, Gerald Koudelka, Herbert Schmidt, Alexander Mellmann, Ulrich Dobrindt, Michael Berger
Summary: This study proposes a new method for genetic manipulation of enterohemorrhagic Escherichia coli (EHEC) that is more efficient than traditional methods. The method increases recombineering efficiency by 3-4 times in EHEC O157:H7 EDL933 Delta stx1/2, but shows similar efficiency in Escherichia coli K-12 MG1655. Additionally, the study suggests that the inhibition of the RecFOR pathway indirectly blocks phage induction and improves recombineering efficiency.
Article
Biochemistry & Molecular Biology
Pierre Millard, Thomas Gosselin-Monplaisir, Sandrine Uttenweiler-Joseph, Brice Enjalbert
Summary: Acetate, previously considered a toxic waste compound, has been found to be a co-substrate of glycolytic nutrients and a global regulator of E. coli metabolism. Decreasing glycolytic flux enhances co-utilization of acetate with glucose, enabling acetate metabolism to compensate for the reduction in glycolytic flux and promote E. coli growth.
Article
Gastroenterology & Hepatology
Nadege Bossuet, Cecile Guyonnet, Camille Chagneau, Min Tang-Fichaux, Marie Penary, Dorian Loubet, Priscilla Branchu, Eric Oswald, Jean-Philippe Nougayrede
Summary: Up to 25% of healthy human feces-derived E. coli strains carry the pks genomic island, which is responsible for synthesizing colibactin, a genotoxic compound implicated in colorectal cancer. However, the expression conditions of colibactin in the gut remain poorly understood. In this study, it is demonstrated that colibactin production is highest under anoxic conditions and decreases with increased oxygen concentration. The positive regulation of colibactin production and genotoxicity by the aerobic respiration control (ArcA) in response to oxygen availability suggests that the pks biosynthetic pathway is adapted for the anoxic intestinal lumen and hypoxic infected or tumor tissue.
Review
Food Science & Technology
Camille Pin, Laure David, Eric Oswald
Summary: Bacteria can release extracellular vesicles, called OMVs, which have diverse functions in host-pathogen interactions during infections. These vesicles have remarkable capabilities, such as disseminating throughout the organism, transporting toxins, and being internalized by eukaryotic cells. They not only impact host cell viability but also interfere with essential cellular machinery.
Article
Endocrinology & Metabolism
Noemie Butin, Cecilia Berges, Jean-Charles Portais, Floriant Bellvert
Summary: This study aimed to develop and validate a method to optimize automated data processing for untargeted MS-based isotopic tracing investigations of metabolism. The method utilizes a suitable reference material to rationally perform parameter optimization throughout the complete data processing workflow. It was applied in the context of C-13-labelling experiments and with two different software, geoRge and X13CMS. The study of an E. coli mutant impaired for central metabolism was used as an example to illustrate the method.
