Review
Multidisciplinary Sciences
Weihan Hou, Yangjie Wei
Summary: This study provides a comprehensive analysis and comparison of various methods for acquiring the point spread function (PSF) in conventional optical microscopy, and proposes two new methods with demonstrated effectiveness. It is of great significance for evaluating and enhancing the imaging resolution of microscopy.
Article
Chemistry, Physical
Megan A. Steves, Kenneth L. Knappenberger
Summary: In this study, Fourier transform nonlinear optical microscopy is used to perform high-resolution nonlinear spectroscopy of single gold nanorods in an imaging platform. By detecting the nonlinear signal, wide-field hyperspectral imaging of the longitudinal plasmon resonances in individual gold nanorods is achieved. Furthermore, the study demonstrates super-resolution capabilities to distinguish multiple nanorods that are spatially separated by only tens of nanometers.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Review
Immunology
Tuula Penate Medina, Jan Philip Kolb, Gereon Huttmann, Robert Huber, Oula Penate Medina, Linh Ha, Patricia Ulloa, Naomi Larsen, Arianna Ferrari, Magdalena Rafecas, Mark Ellrichmann, Mariya S. Pravdivtseva, Mariia Anikeeva, Jana Humbert, Marcus Both, Jennifer E. Hundt, Jan-Bernd Hovener
Summary: This article reviews the recent advances in imaging technologies and their potential applications in the medical field, focusing on the development of imaging in precision medicine for inflammation. It also analyzes the challenges and opportunities faced by imaging technologies, showcasing some current or near-clinical applications as examples.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Nonthanan Sitpathom, Sujin Suwanna, Wanchai Pijitrojana, Yingyot Infahsaeng, Ekkarat Pongophas, Ruchipas Bavontaweepanya, Tanapat Deesuwan
Summary: Material modification on plasmonic nanostructures can enhance the optical near-field enhancement and resonance wavelength tuning. This study focuses on closed-hexagonal sub-micro disk arrays of the AgxAu1-x alloy and pure Ag arrays, and shows that the electric-field enhancement is increased in the near-infrared and visible spectra, respectively. Additionally, the combination of Ag/Au heteromaterial array of sub-micro disks strengthens the local confinement in the near-infrared region and boosts the electric field enhancement.
OPTICAL MATERIALS EXPRESS
(2022)
Review
Optics
Minfei He, Zhimin Zhang, Chun Cao, Guozun Zhou, Cuifang Kuang, Xu Liu
Summary: Photoinhibition lithography (PIL) is a nanoscale fabrication technique that enables super-resolution 3D printing through the control and confinement of exposed regions for photoinitiation with multicolor visible light. It has diverse applications in photonics, data storage, and biotechnology, with ongoing research on sub-diffraction printing and high-throughput fabrication. Challenges and potential solutions are being discussed to drive technological breakthroughs in PIL for improved resolution and throughput.
LASER & PHOTONICS REVIEWS
(2022)
Article
Cell Biology
Laura Zoe Kreplin, Senthil Arumugam
Summary: Live-cell imaging plays a crucial role in researching organelle dynamics, cytoskeletal interactions, membrane protrusions, and cell motility. In recent years, various light-sheet geometries have been developed to push the boundaries of measurements.
CURRENT OPINION IN CELL BIOLOGY
(2023)
Article
Biochemical Research Methods
Jeffrey Demas, Jason Manley, Frank Tejera, Kevin Barber, Hyewon Kim, Francisca Martinez Traub, Brandon Chen, Alipasha Vaziri
Summary: Two-photon microscopy has enabled high-resolution imaging of neuroactivity within scattering brain tissue, but there are still tradeoffs between speed and spatiotemporal sampling. Light beads microscopy (LBM) introduces a scalable and spatiotemporally optimal acquisition approach for recording neuroactivity at different scales, offering opportunities for discovering neurocomputations in the mammalian brain.
Article
Chemistry, Multidisciplinary
Shuai Zhang, Shanggeng Li, Xiangyu Wan, Ning Li, Jing Li, Qiang Yin, Lin Zhang
Summary: This paper designs and synthesizes a series of new initiators based on coumarin groups, studies and discusses their optical properties and two-photon polymerization properties in detail, and analyzes the relationship between their structures and properties. The synthesized initiators can achieve a two-photon polymerization process with a speed of 100,000 mm/s under a laser power of 35-50 mW, and successfully obtain a series of clear three-dimensional structures with subwavelength resolution. The paper also discusses the relationship between the principle of two-photon polymerization, laser power, writing speed and resolution, establishes two simple mathematical models, obtains a fitting formula with physical meaning, which provides a way for the prediction and control of resolution. Finally, a low-density ring with subwavelength resolution, with an outer diameter of 2.4 mm and an inner diameter of 2.0 mm, was manufactured in just over 10 minutes for the fusion target experiment, proving the possibility of using the resulting initiator to manufacture precision micro-nano devices.
MATERIALS TODAY CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Arun Jaiswal, Sweta Rani, Gaurav Pratap Singh, Mahbub Hassan, Aklima Nasrin, Vincent G. Gomes, Sumit Saxena, Shobha Shukla
Summary: The study introduces a two-photon patternable all carbon material system for fabricating fluorescent 3D micro/nanostructures, addressing the bottleneck of material selection in fabrication of engineered 3D nanostructures. The research successfully achieved two-photon polymerization of acrylate monomers without the need for traditional two-photon absorbing materials through carbon dots under two-photon laser excitation.
Article
Chemistry, Multidisciplinary
Sven Fritzsche, Gerd-Ruediger Jaenisch, Lina Pavasaryte, Alexander Funk
Summary: The article introduces the methods of direct laser writing (DLW) and X-ray computed tomography (XCT) for manufacturing and analyzing polymer structures at the microscale. Through experiments, the article demonstrates the detection and analysis capability of XCT for internal defects in the manufactured structures, as well as the three-dimensional reconstruction of hidden features of the structures. It also shows the advantage of direct fabrication on PET substrate.
APPLIED SCIENCES-BASEL
(2022)
Article
Physics, Applied
Xudong He, Jin Chen, Mingji Chen, Hongchen Chu, Yun Lai, Daining Fang
Summary: This paper proposes a 3D-printed ultra-broadband planar Luneburg lens composed of periodic gradient structures, with a large operating frequency range. It demonstrates high efficiency achromatic sub-diffraction focusing and highly directive far-field radiation pattern.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2022)
Article
Nanoscience & Nanotechnology
Jongyoun Kim, Inkook Hwang, Minkyoung Kim, Hyeonwoo Jung, Hyejeong Bae, Youngu Lee
Summary: This study reports a novel reverse-offset printing technology for high-resolution micro-patterning of copper nanowires. By adjusting the ink formulation, high-quality copper nanowire micro-shapes were achieved, demonstrating the potential for excellent performance in flexible transparent electronic devices.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Physics, Applied
F. Gomez, R. S. Dutra, L. B. Pires, Glauber R. de S. Araujo, B. Pontes, P. A. Maia Neto, H. M. Nussenzveig, N. B. Viana
Summary: Researchers established an explicit partial-wave series for the image of dielectric microspheres collected by a typical infinity-corrected microscope, incorporating the angular-spectrum theorem and Wigner rotation matrix elements. They considered a high numerical aperture objective and spherical aberration introduced by refraction, ensuring accurate characterization of the microspheres. The theoretical model was validated through comparisons with experimental data, demonstrating good agreement in measuring particle properties.
PHYSICAL REVIEW APPLIED
(2021)
Article
Optics
Won-yong Ha, Eunsung Park, Doyoon Eom, Hyo-Sung Park, Daniel Chong, Shyue Seng Tan, Michelle Tng, Elgin Quek, Claudio Bruschini, Edoardo Charbon, Woo-Young Choi, Myung-Jae Lee
Summary: This paper presents a SPAD in 55 nm BCD technology, which achieves a breakdown voltage of 18.4 V and a dark count rate of 4.4 cps/mu m2 at 7 V excess bias voltage. The device also achieves a high PDP of 70.1% at 450 nm and PDP values of 7.2% and 3.1% at 850 and 940 nm, respectively, for 3D ranging applications. The SPAD has a timing jitter of 91 ps at 850 nm and is expected to enable cost-effective time-of-flight and LiDAR sensors for mobile applications.
Article
Materials Science, Multidisciplinary
Pooria Lesani, Aina Hazeera Mohamad Hadi, Mansi Khetarpaul, Zufu Lu, Elizabeth J. New, Hala Zreiqat
Summary: In recent years, the development of carbon dot-based fluorescent nanoparticles for bioimaging applications has gained attention. This study reports the design and development of efficient two-photon excitable red-emissive carbon dots, highlighting their potential in bioimaging. The importance of aromatic precursors in developing red-emissive carbon dots is demonstrated. The optimized carbon dots are biocompatible, non-toxic, and photostable under two-photon excitation. This study highlights the great potential of these carbon dots as efficient bioimaging agents for cellular labeling, real-time imaging, and deep-tissue imaging in complex biological systems.
ADVANCED PHOTONICS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Cecilia P. Sanchez, Pintu Patra, Shih-Ying Scott Chang, Christos Karathanasis, Lukas Hanebutte, Nicole Kilian, Marek Cyrklaff, Mike Heilemann, Ulrich S. Schwarz, Mikhail Kudryashev, Michael Lanzer
Summary: KAHRP plays a key role in Plasmodium falciparum malaria by forming membrane protrusions in infected erythrocytes, anchoring parasite-encoded adhesins to the membrane skeleton. Through super-resolution microscopy, it was found that KAHRP initially associates with various skeletal components before eventually colocalizing with remnant actin junctions under the spiral scaffold forming knobs. Additionally, a dynamic model of KAHRP organization and its function in attaching other factors to the spiral scaffold was proposed based on the findings.
MOLECULAR MICROBIOLOGY
(2022)
Article
Cell Biology
Sebastian Malkusch, Johanna Rahm, Marina S. Dietz, Mike Heilemann, Jean-Baptiste Sibarita, Jorn Lotsch
Summary: This study proposes a method that analyzes the differences in receptor mobility between internalin B-treated and -untreated cells. By using hidden Markov modeling and explainable artificial intelligence, the key differences in MET under different treatment conditions are learned from single-particle tracking data, allowing for inference of cellular information.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Engineering, Biomedical
T. N. M. Hoang, Z. Cseresnyes, S. Hartung, M. Blickensdorf, C. Saffer, K. Rennert, A. S. Mosig, M. von Lilienfeld-Toal, M. T. Figge
Summary: Invasive pulmonary aspergillosis is a serious infection with a high mortality rate. The invasive aspergillosis-on-chip (IAC) model allows the study of fungal growth and invasion in vitro. The model uses an algorithm-based analysis pipeline to visualize and quantify the morphology of the fungus. Macrophages in the IAC model inhibit fungal growth partially and contribute to the release of proinflammatory cytokines and chemokines. The fungistatic drug caspofungin limits fungal growth and causes morphological changes in the hyphal tree.
Article
Multidisciplinary Sciences
Shivam Yadav, Martin Centola, Mathilda Glaesmann, Denys Pogoryelov, Roman Ladig, Mike Heilemann, L. C. Rai, Ozkan Yildiz, Enrico Schleiff
Summary: This study reveals the role of a cyanobacterial cyclophilin in stress responses and assembly of photosynthetic complexes, and presents its unique structural features.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Nicole Koller, Philipp Hoellthaler, Martina Barends, Marius Doering, Christoph Spahn, Veronica Duran, Bibiana Costa, Jennifer Becker, Mike Heilemann, Ulrich Kalinke, Robert Tampe
Summary: Dendritic cells (DCs) translate innate immune responses into adaptive immunity, but the spatial organization of underlying processes is not well understood. By studying human monocyte-derived DCs, we discovered a nanoscale organization of antigen-loading machinery and observed an increase in density during DC maturation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Julian Kompa, Jorick Bruins, Marius Glogger, Jonas Wilhelm, Michelle S. Frei, Miroslaw Tarnawski, Elisa D'Este, Mike Heilemann, Julien Hiblot, Kai Johnsson
Summary: The specific and covalent labeling of the protein HaloTag with fluorescent probes in living cells makes it a powerful tool for bioimaging. However, the irreversible attachment of the probe to HaloTag precludes imaging applications that require transient binding of the probe and comes with the risk of irreversible photobleaching. Here, we introduce exchangeable ligands for fluorescence labeling of HaloTag (xHTLs) that reversibly bind to HaloTag and that can be coupled to rhodamines of different colors.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Chemistry, Multidisciplinary
Lorenzo Albertazzi, Mike Heilemann
Summary: The exploitation of low-affinity molecular interactions in protein labeling is an emerging topic in optical microscopy, which allows for a constant renewal of fluorescence signals at target sites. It has versatile use across microscopy methods and has shown potential in 3D, live, and many-target applications. Although some progress has been made in developing low-affinity labels, this research field still has a lot of untapped potential.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Engineering, Biomedical
WanLing Foo, Zoltan Cseresnyes, Carsten Roessel, Yingfeng Teng, Anuradha Ramoji, Mingzhe Chi, Walter Hauswald, Sophie Huschke, Stephanie Hoeppener, Juergen Popp, Felix H. Schacher, Marek Sierka, Marc Thilo Figge, Adrian T. Press, Michael Bauer
Summary: Targeting delivery of oligonucleotides or small molecular drugs to hepatocytes is challenging due to the efficient mononuclear phagocyte system of the liver. In this study, polyplex micelles were used as nanocarriers for drug delivery. By altering the ratio of poly(ethylene glycol) corona to siRNA complexed poly(allyl glycidyl ether) core, the micelles were able to overcome the macrophage-rich reticuloendothelial barrier and specifically target hepatocytes.
Article
Biochemistry & Molecular Biology
Claudia Catapano, Johanna V. Rahm, Marjan Omer, Laura Teodori, Jorgen Kjems, Marina S. Dietz, Mike Heilemann
Summary: HER2 belongs to the ErbB sub-family of receptor tyrosine kinases and is activated through heterodimerization with other ErbB receptors. This study found that HER2 is strongly activated by EGFR-targeting ligands EGF and TGFa, with a distinguishable temporal fingerprint. HER4-targeting ligands EREG and NRG beta 1 showed weaker activation of HER2, with a preference for EREG and a delayed response to NRG beta 1.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Fengjiao Ma, Rainer Kaufmann, Jaroslaw Sedzicki, Zoltan Cseresnyes, Christoph Dehio, Stephanie Hoeppener, Marc Thilo Figge, Rainer Heintzmann
Summary: Correlative light and electron microscopy combines the benefits of both techniques to study cell structure. However, the current method of overlaying light microscopy onto electron microscopy is limited due to differences in structural detail. This paper introduces a new approach called EM-guided deconvolution to bridge the resolution and specificity gaps. The method was tested on simulations, multi-color beads, and biological samples.
Article
Cell & Tissue Engineering
Atiyeh Asadpour, Badrul Hisham Yahaya, Katrina Bicknell, Graeme S. Cottrell, Darius Widera
Summary: The direct-to-consumer industry for secretome-based therapies is growing rapidly without appropriate regulation and guidelines. Many businesses are offering secretome-based interventions without sufficient evidence, putting patients at risk and potentially damaging the credibility of the field.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Chemistry, Multidisciplinary
Laurell F. F. Kessler, Ashwin Balakrishnan, Nina S. S. Deussner-Helfmann, Yunqing Li, Maximilian Mantel, Marius Glogger, Hans-Dieter Barth, Marina S. S. Dietz, Mike Heilemann
Summary: Super-resolution techniques such as SMLM and STED microscopy are enhanced by using weak affinity-based transient labelling systems, such as DNA-based hybrid systems. However, these techniques suffer from high background fluorescence signal caused by unbound fluorophore-labelled oligonucleotides. This study introduces short-distance self-quenching in fluorophore dimers as an efficient mechanism to reduce background fluorescence signal and increase photon budget in the bound state.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Alon Saguy, Onit Alalouf, Nadav Opatovski, Soohyen Jang, Mike Heilemann, Yoav Shechtman
Summary: Single-molecule localization microscopy (SMLM) has significantly improved spatial resolution in biological imaging, but has limitations in observing dynamics at high temporal resolution. In this study, we introduce DBlink, a deep-learning-based method that reconstructs super spatiotemporal resolution videos from SMLM data. DBlink utilizes a convolutional neural network combined with a bidirectional long short-term memory network to capture long-term dependencies between different frames. Experimental results demonstrate the effectiveness of DBlink in various scenarios, including simulated structures and live-cell dynamic SMLM. This advancement in super-resolution imaging of dynamic processes in living cells is crucial.
Article
Biophysics
Soohyen Jang, Kaarjel K. Narayanasamy, Johanna V. Rahm, Alon Saguy, Julian Kompa, Marina S. Dietz, Kai Johnsson, Yoav Shechtman, Mike Heilemann
Summary: Single-molecule localization microscopy achieves nanometer spatial resolution but faces challenges of long acquisition time and photobleaching. This study combines a neural network and a protein labeling method to improve acquisition speed and temporal resolution, enabling capturing of cellular structure dynamics in live cells.
BIOPHYSICAL REPORTS
(2023)
Proceedings Paper
Cell Biology
Johanna Rahm, Sebastian Malkusch, Ulrike Endesfelder, Marina S. Dietz, Mike Heilemann
Summary: This study presents a pipeline for the analysis of single-particle tracking data, allowing for the determination of biomolecule mobility and diffusion state transitions. The application of this pipeline to receptor trajectories revealed that activated receptors move slower and more confined, with more transitions from free to confined diffusion states. The analysis also identified three diffusion states, with increased transition probabilities towards the less mobile and immobile states upon ligand activation.
SINGLE MOLECULE SPECTROSCOP AND SUPERRESOLUTION IMAGING XV
(2022)
