Article
Medicine, Research & Experimental
Chao Zhang, Zhao Yan, Asif Maknojia, Manuel A. Riquelme, Sumin Gu, Grant Booher, David J. Wallace, Viktor Bartanusz, Akshay Goswami, Wei Xiong, Ningyan Zhang, Michael J. Mader, Zhiqiang An, Naomi L. Sayre, Jean X. Jiang
Summary: An inhibitory antibody, MHC1, specifically targeting Cx43 hemichannels, was developed to reduce secondary damage after spinal cord injury. Administration of MHC1 improved hind limb locomotion function in SCI mouse models and showed long-lasting effects up to 8 weeks post-SCI, indicating a potential innovative therapeutic approach for SCI treatment.
Article
Biology
Ji Xu, Bruce J. Nicholson
Summary: This study investigated the characteristics of Cx30 hemichannels using heterologous expression systems. It was found that Cx30 hemichannels are sensitive to voltage and extracellular Ca2+ and show minimal selectivity for small ions but increased permeability for larger anions. Comparison with Cx26 gap junctions in the cochlea revealed differences in ATP permeability, indicating distinct physiological functions of Cx30 and Cx26 in energy distribution.
Article
Biochemistry & Molecular Biology
Ivanka Jimenez-Dinamarca, Rachel Reyes-Lizana, Yordan Lemunao-Inostroza, Kevin Cardenas, Raimundo Castro-Lazo, Francisca Pena, Claudia M. Lucero, Juan Prieto-Villalobos, Mauricio Antonio Retamal, Juan Andres Orellana, Jimmy Stehberg
Summary: GABA plays a crucial role in regulating the excitatory/inhibitory balance in the brain by affecting the activity of neurons and astrocytes through different GABA receptors. This study specifically focuses on how GABA influences astroglial Cx43 hemichannel activity and its release of glutamate and ATP, providing insights into potential mechanisms underlying psychiatric disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Ross Luu, Silvana Valdebenito, Eliana Scemes, Antonio Cibelli, David C. Spray, Maximiliano Rovegno, Juan Tichauer, Andrea Cottignies-Calamarte, Arielle Rosenberg, Calude Capron, Sandrine Belouzard, Jean Dubuisson, Djillali Annane, Geoffroy Lorin de la Grandmaison, Elisabeth Cramer-Borde, Morgane Bomsel, Eliseo Eugenin
Summary: The study found that SARS-CoV-2 induces a more significant and prolonged opening of Panx-1 channels compared to other viruses, leading to increased release of ATP, PGE(2), and IL-1 beta. Therefore, the Panx-1 channel is considered a critical factor in SARS-CoV-2 infection and could be a potential alternative therapy.
Article
Multidisciplinary Sciences
Hyun-Bum Kim, Jacqueline Morris, Kevin Miyashiro, Tonis Lehto, Ulo Langel, James Eberwine, Jai-Yoon Sul
Summary: The research found that astrocytes can generate a stronger calcium signal response after acute ethanol exposure, primarily influenced by neuronally-derived purinergic signaling. Astrocytes adapt to the effects of ethanol by modulating the activity of adenylyl cyclase and phospholipase C, highlighting their important role in shaping the overall physiological responsiveness to ethanol.
Article
Multidisciplinary Sciences
Hang Zhang, Shiyu Wang, Zhenzhen Zhang, Mengzhuo Hou, Chunyu Du, Zhenye Zhao, Horst Vogel, Zhifang Li, Kaige Yan, Xiaokang Zhang, Jianping Lu, Yujie Liang, Shuguang Yuan, Daping Wang, Huawei Zhang
Summary: Pannexin 2 plays critical roles in many physiological processes but its mechanism remains unclear. In this study, the cryo-EM structure of human Panx2 in the open state is reported, and critical residues for its gating are identified.
NATURE COMMUNICATIONS
(2023)
Article
Neurosciences
Daniela Madeira, Liliana Dias, Patricia Santos, Rodrigo A. Cunha, Paula M. Canas, Paula Agostinho
Summary: The study identifies a feed-forward loop involving astrocytic A(2A)R and Cx43 hemichannels, where A(2A)R increases Cx43 hemichannel activity leading to increased ATP release, which is converted into adenosine by CD73, sustaining the increased astrocytic A(2A)R activity in AD-like conditions.
MOLECULAR NEUROBIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Nicholas Dale
Summary: This review emphasizes the importance of real-time measurements of extracellular ATP and adenosine concentrations, provides additional mechanistic insights, and outlines current techniques available for spatially localized measurements. The examples of CO2 and nutrient sensing highlight the impact of direct real-time measurements, demonstrating the role of purinergic signaling in breathing control, weight regulation, and sleep-wake state. Additionally, the complex mechanisms of activity-dependent adenosine release in different brain regions are discussed, underscoring the potential of whole blood purine measurements in clinical practice.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lina Mariana Tovar, Carlos Felipe Burgos, Gonzalo E. Yevenes, Gustavo Moraga-Cid, Jorge Fuentealba, Claudio Coddou, Luisa Bascunan-Godoy, Claudio Catrupay, Angel Torres, Patricio A. A. Castro
Summary: Neurulation is a crucial process in the formation of the central nervous system (CNS). Connexins (Cxs), which form gap junctions (GJs) and hemichannels (HCs), play a role in neural tube development. ATP release mediated by HCs-Cxs is important during the neurulation process. Blockade of HCs-Cxs can lead to neural tube defects (NTDs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Juan Prieto-Villalobos, Claudia M. Lucero, Maximiliano Rovegno, Gonzalo I. Gomez, Mauricio A. Retamal, Juan A. Orellana
Summary: This study found that the spike protein S1 of SARS-CoV-2 can increase the activity of Cx43 hemichannels, potentially leading to cellular dysfunction and damage observed in COVID-19 patients. Therefore, investigating Cx43 hemichannels may provide insights for developing therapies against viral infection.
BIOLOGICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Hui Song Cui, So Young Joo, Yoon Soo Cho, Ji Heon Park, Yu Mi Ro, June-Bum Kim, Cheong Hoon Seo
Summary: The study aimed to investigate the molecular mechanisms of extracorporeal shock wave therapy on keratinocytes derived from hypertrophic scars. The results showed that shock waves altered the proliferation, activation, differentiation, and apoptosis of cells, as well as regulating proliferation/differentiation factors, maintaining normal epidermal integrity.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Flora Vasile, Elena Dossi, Julien Moulard, Pascal Ezan, Laure Lecoin, Martine Cohen-Salmon, Philippe Mailly, Marc Le Bert, Isabelle Couillin, Alexis Bemelmans, Nathalie Rouach
Summary: Activation of astroglial Pannexin 1 channels plays a crucial role in the inhibition of hippocampal neuronal networks through a negative feedback mechanism, by regulating purinergic signaling-mediated HCN-gated channels to decrease neuronal excitability.
Article
Biochemistry & Molecular Biology
Yutaka Shindo, Keigo Fujita, Mari Tanaka, Hiroki Fujio, Kohji Hotta, Kotaro Oka
Summary: Recent studies have shown that keratinocytes play crucial roles in nociception and ATP is a key mediator in signal transduction from keratinocytes to sensory neurons. Sensory dorsal root ganglion (DRG) neurons are more sensitive to ATP than keratinocytes, and signals from moderate mechanical stimulation of keratinocytes are predominantly transmitted to DRG neurons.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Carina Herman de Sousa, Maria Adelina Costa, Rafaela Pedro Silva, Fatima Ferreirinha, Severino Ribeiro, Paulo Correia-de-Sa
Summary: This study demonstrates for the first time the overexpression of Panx1 in HSCF induced by A(2A)R, indirectly implicating ATP release in the fibrogenic vicious cycle operated by adenosine accumulating in subcutaneous tissue fibrosis and myofascial pain associated with dermal inflammation.
Article
Dermatology
Syahla Nisaa Amalia, Hritu Baral, Chisako Fujiwara, Akihiko Uchiyama, Yuta Inoue, Sahori Yamazaki, Mai Ishikawa, Keiji Kosaka, Akiko Sekiguchi, Yoko Yokoyama, Sachiko Ogino, Ryoko Torii, Mari Hosoi, Koji Shibasaki, Sei-ichiro Motegi
Summary: This study investigated the role of TRPV4 in psoriasis. High expression of TRPV4 was found in keratinocytes of human psoriasis skin and in an imiquimod-induced psoriasis-like mouse model. Knockout of TRPV4 in mice resulted in less severe dermatitis, reduced epidermal thickness, and decreased infiltration of immune cells. Knockout of TRPV4 also suppressed the release of adenosine triphosphate and the secretion of neuropeptides, providing protection against psoriasis progression.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Olga A. Akimova, Artem M. Tverskoi, Larisa V. Smolyaninova, Alexander A. Mongin, Olga D. Lopina, Jennifer La, Nickolai O. Dulin, Sergei N. Orlov
Article
Biochemistry & Molecular Biology
Alexandra L. Schober, Alexander A. Mongin
JOURNAL OF NEUROCHEMISTRY
(2015)
Review
Physiology
Alexander A. Mongin
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2016)
Article
Neurosciences
Preeti Dohare, Muhammad T. Zia, Ehsan Ahmed, Asad Ahmed, Vivek Yadala, Alexandra L. Schober, Juan Alberto Ortega, Robert Kayton, Zoltan Ungvari, Alexander A. Mongin, Praveen Ballabh
JOURNAL OF NEUROSCIENCE
(2016)
Article
Neurosciences
Alexandra L. Schober, Dmitriy A. Gagarkin, Ying Chen, Guangping Gao, Lauren Jacobson, Alexander A. Mongin
FRONTIERS IN CELLULAR NEUROSCIENCE
(2016)
Article
Neurosciences
Alexandra L. Schober, Corinne S. Wilson, Alexander A. Mongin
JOURNAL OF PHYSIOLOGY-LONDON
(2017)
Review
Neurosciences
Corinne S. Wilson, Alexander A. Mongin
NEUROSCIENCE LETTERS
(2019)
Article
Oncology
Sebastian Rubino, Martin D. Bach, Alexandra L. Schober, Ian H. Lambert, Alexander A. Mongin
FRONTIERS IN ONCOLOGY
(2018)
Article
Neurosciences
Maria C. Hyzinski-Garcia, Alena Rudkouskaya, Alexander A. Mongin
JOURNAL OF PHYSIOLOGY-LONDON
(2014)
Article
Biochemistry & Molecular Biology
Corinne S. Wilson, Martin D. Bach, Zahra Ashkavand, Kenneth R. Norman, Nina Martino, Alejandro P. Adams, Alexander A. Mongin
JOURNAL OF NEUROCHEMISTRY
(2019)
Article
Virology
Jesse Rabinowitz, Hamayun J. Sharifi, Hunter Martin, Anthony Marchese, Michael Robek, Binshan Shi, Alexander A. Mongin, Carlos M. C. de Noronha
Summary: Human macrophages have intrinsic antiviral defenses against HIV-1 infection, with Nrf2 and xCT playing key roles in inhibiting the virus. xCT function impedes infection immediately prior to 2-LTR circle formation.
Article
Biochemistry & Molecular Biology
Corinne S. Wilson, Preeti Dohare, Shaina Orbeta, Julia W. Nalwalk, Yunfei Huang, Russell J. Ferland, Rajan Sah, Annalisa Scimemi, Alexander A. Mongin
Summary: The knockout of the LRRC8A gene in mice leads to seizures in adolescence, as well as abnormalities in neurons and astrocytes in the brain. These abnormalities are associated with reactive astrogliosis, as well as dysregulation of amino acid neurotransmitter uptake and supply by astrocytes.
Editorial Material
Cell Biology
Markus Ritter, Alexander A. Mongin, Giovanna Valenti, Yasunobu Okada
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Physiology
Alexander A. Mongin
Meeting Abstract
Critical Care Medicine
N. O. Dulin, A. A. Mongin, S. Orbeta, E. B. Reed, G. M. Mutlu
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)