Article
Multidisciplinary Sciences
Marie-Claire Rittmann, Saskia Hussung, Lukas M. Braun, Rhena F. U. Klar, Esther A. Biesel, Stefan Fichtner-Feigl, Ralph Fritsch, Uwe A. Wittel, Dietrich A. Ruess
Summary: The study aimed to identify preoperative plasma protein biomarkers in PDAC patients to predict early recurrence after surgery. 23 proteins showed differential abundance in perioperative plasma between early and late recurrence patients. MAEA and NT5E were the most promising candidates for further evaluation.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Jillian W. P. Bracht, Mandy Los, Monique A. J. van Eijndhoven, Britta Bettin, Edwin van der Pol, D. Michiel Pegtel, Rienk Nieuwland
Summary: This study investigated whether the analysis of EV-miRNA is affected by the presence of platelets and ery-ghosts in human plasma. The results showed that filtration of plasma can remove platelets and ery-ghosts, improving the comparability and reproducibility of EV-miRNA studies. The authors recommend measuring and reporting the plasma concentration of platelets for EV-miRNA studies, and filtering plasma before downstream analyses or storage in biobanks.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Medicine, General & Internal
Da Huang, Hui Qi, Hongchun Yang, Meng Chen
Summary: This study identified several plasma secretory miRNAs closely related to the development of MMD, which can be used as biomarkers for predicting MMD with high sensitivity and specificity. These miRNAs can differentiate MMD from non-MMD patients before digital subtraction angiography.
Article
Biochemistry & Molecular Biology
Donatella Coradduzza, Tatiana Solinas, Emanuela Azara, Nicola Culeddu, Sara Cruciani, Angelo Zinellu, Serenella Medici, Margherita Maioli, Massimo Madonia, Ciriaco Carru
Summary: This study evaluated the potential of agmatine as a biomarker for distinguishing different classes of prostate cancer patients. The results showed that using polyamine levels can effectively discriminate between the three patient groups. Agmatine levels demonstrated high AUC values and good sensitivity, specificity, and accuracy.
Review
Biochemistry & Molecular Biology
Kah Yee Goh, Terence You De Cheng, Su Chin Tham, Darren Wan-Teck Lim
Summary: Non-small cell lung cancer (NSCLC) is the dominant form of lung cancer and typically has a poor prognosis. Immunotherapy using immune checkpoint inhibitors (ICI) has shown superior efficacy compared to conventional chemotherapy for advanced NSCLC patients. However, there is a need for biomarkers to accurately predict immunotherapy response, as only a minority of patients benefit from this treatment. Liquid biopsy, which enables non-invasive sampling of blood-based biomarkers, allows for the dynamic monitoring of treatment response. This review summarizes the progress and challenges in identifying circulating biomarkers for predicting immunotherapy benefit in NSCLC patients.
Article
Multidisciplinary Sciences
Janhavi R. Raut, Ben Schoettker, Bernd Holleczek, Feng Guo, Megha Bhardwaj, Kaya Miah, Petra Schrotz-King, Hermann Brenner
Summary: This study proposes a blood-based microRNA signature for identifying high-risk populations for colorectal cancer screening. Circulating microRNAs (miRNAs) are found to improve colorectal cancer risk prediction, with a 7-miRNA score showing higher predictive ability compared to environmental and polygenic risk scores.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Jia Liu, Binglin Chen, Man Yang, Yun Qian, Qian Shen, Hai Chen, Yunqiu Dong, Lu Wang, Jiandong Jiao
Summary: This study conducted a nested case-control study in a community-based population in China and identified three plasma miRNAs (miRNA-29a, miRNA-125b, miRNA-145) significantly associated with colorectal cancer risk, which may serve as potential biomarkers for early detection and individual risk prediction of colorectal cancer.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Yasmina Chouik, Fanny Lebosse, Marie-Laure Plissonnier, Jean-Christophe Lega, Pierre Pradat, Teresa Antonini, Miroslava Subic, Kerstin Hartig-Lavie, Domitille Erard, Francois Villeret, Celine Guichon, Audrey Payance, Sylvie Radenne, Pierre-Emmanuel Rautou, Fabien Zoulim, Massimo Levrero
Summary: Bacterial infections are a common and dangerous complication in patients with acute decompensation of cirrhosis. This study investigated the potential of circulating microRNAs to diagnose bacterial infections and predict survival in these patients. The analysis found that certain miRNAs can be used as biomarkers to accurately diagnose bacterial infections, and a combination of other miRNAs can help predict survival. Circulating miRNAs show dysregulation in patients with acute decompensation of cirrhosis, suggesting their potential as diagnostic and prognostic tools.
Article
Oncology
Marie Wislez, Julien Mazieres, Armelle Lavole, Gerard Zalcman, Olivier Carre, Thomas Egenod, Raffaele Caliandro, Catherine Dubos-Arvis, Gaelle Jeannin, Olivier Molinier, Marie-Ange Massiani, Alexandra Langlais, Franck Morin, Francoise Le Pimpec Barthes, Laurent Brouchet, Jalal Assouad, Bernard Milleron, Diane Damotte, Martine Antoine, Virginie Westeel
Summary: This study assessed the feasibility of neoadjuvant durvalumab for early-stage resectable non-small-cell lung cancer. The results showed that this treatment approach resulted in a high complete resection rate and some pathological response. However, the unexpectedly high postoperative mortality rate led to the premature termination of the study.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Review
Oncology
Andrea York Tiang Teo, Vivian Yujing Lim, Valerie Shiwen Yang
Summary: Soft tissue sarcoma is a rare cancer that accounts for 1% of adult cancers and 20% of pediatric solid tumors. Prognosis in advanced disease is poor. MicroRNAs (miRNAs) are short non-coding RNAs that control gene expression and can be used for prognostication, treatment resistance identification, and new therapeutics in soft tissue sarcomas.
Article
Oncology
Bolin Wang, Bing Zou, Shengnan Xu, Chao Zhao, Jinli Pei, Shijie Wang, Kunlong Zhao, Jinming Yu, Jie Liu
Summary: This study aimed to investigate the role of ctDNA detection in estimating prognosis and guiding adjuvant therapy (ADT) for resectable stage I NSCLC patients. The results showed that ctDNA-negative patients had a significantly lower risk of recurrence compared to ctDNA-positive patients, but there was no difference in the risk of death between the two groups. In the ctDNA-positive group, there was no significant difference in relapse-free survival (RFS) between patients who received ADT and patients who did not receive ADT. However, in the ctDNA-negative group, patients who received ADT had a worse RFS compared to those who did not receive ADT. Therefore, ctDNA detection can serve as a prognostic marker for predicting recurrence but has limited efficacy in guiding ADT for resectable stage I NSCLC.
FRONTIERS IN ONCOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Julia Faura, Alejandro Bustamante, Silvia Reverte, Teresa Garcia-Berrocoso, Monica Millan, Mar Castellanos, Blanca Lara-Rodriguez, Josep Zaragoza, Oriol Ventura, Maria Hernandez-Perez, Cecile van Eendenburg, Pere Cardona, Elena Lopez-Cancio, David Canovas, Joaquin Serena, Marta Rubiera, Antoni Davalos, Joan Montaner
Summary: The study confirmed that blood biomarker panels can early predict acute decompensated heart failure and respiratory tract infections in patients with stroke, which may help clinicians manage complications promptly.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Review
Biochemistry & Molecular Biology
Jorge Armando Jimenez-Avalos, Juan Carlos Fernandez-Macias, Ana Karen Gonzalez-Palomo
Summary: Exosomes, particularly the miRs within them, have the potential to serve as non-invasive biomarkers for the diagnosis and prognosis of various diseases, making them valuable tools for disease management and early detection.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Nutrition & Dietetics
Lidia Daimiel, Victor Mico, Laura Diez-Ricote, Paloma Ruiz-Valderrey, Geoffrey Istas, Ana Rodriguez-Mateos, Jose Maria Ordovas
Summary: This pilot study found that the consumption of beer and non-alcoholic beer can differently modulate the levels of cardiometabolic microRNAs in plasma and macrophages, with microRNAs related to inflammation showing opposite trends after beer consumption compared to non-alcoholic beer consumption.
Article
Oncology
Michela Saviana, Giulia Romano, Joseph McElroy, Giovanni Nigita, Rosario Distefano, Robin Toft, Federica Calore, Patricia Le, Daniel Del Valle Morales, Sarah Atmajoana, Stephen Deppen, Kai Wang, L. James Lee, Mario Acunzo, Patrick Nana-Sinkam
Summary: This study proposes a miRNA-based biomarker classifier for SCLC that considers clinical demographics with specific cut offs to inform SCLC diagnosis.
FRONTIERS IN ONCOLOGY
(2023)
Article
Cell Biology
Tanja Niedermair, Meet Bhatt, Maximilian Babel, Moritz Feustel, Andreas Mamilos, Helmut Schweikl, Gerlinde Ferstl, Paul Hofman, Christoph Brochhausen
Summary: One major goal of biobanks is to provide optimal biospecimen quality, achieved through standardized procedures for collection, processing, and storage. Temporary storage of biosamples is necessary for maintaining sample quality, with considerations for dewar size, maximum storage time, and cryotube material.
BIOPRESERVATION AND BIOBANKING
(2023)
Editorial Material
Genetics & Heredity
Patrick Brest, Baharia Mograbi, Jocelyn Gal, Paul Hofman, Gerard Milano
Summary: Since the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), studies have shown that host genetic background may contribute to the development of severe COVID-19. This summary focuses on genetic variations, such as in the SARS-CoV-2 receptor angiotensin-converting enzyme 2 or interferon signaling pathway, and their role in understanding why some individuals can develop severe COVID-19.
TRENDS IN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Marius Ilie, Jonathan Benzaquen, Veronique Hofman, Elodie Long-Mira, Sandra Lassalle, Jacques Boutros, Christophe Bontoux, Virginie Lespinet-Fabre, Olivier Bordone, Virginie Tanga, Maryline Allegra, Myriam Salah, Julien Fayada, Sylvie Leroy, Matteo Vassallo, Irit Touitou, Johan Courjon, Julie Contenti, Michel Carles, Charles-Hugo Marquette, Paul Hofman
Summary: As new SARS-CoV-2 variants emerge, it is important to improve the efficiency and availability of viral genome sequencing, especially in samples with low viral load. In this study, retrospective SARS-CoV-2 genome sequencing was performed on 175 positive samples, and the analysis successfully detected Delta and Omicron variants in low viral load samples. The results showed that there was no significant difference in viral load between patients infected with the Omicron variant and those infected with the Delta variant.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Olivia Vidal-Cruchez, Victoria J. Nicolini, Tifenn Rete, Karine Jacquet, Roger Rezzonico, Caroline Lacoux, Marie-Angela Domdom, Barnabe Romeo, Jeremie Roux, Arnaud Hubstenberger, Bernard Mari, Baharia Mograbi, Paul Hofman, Patrick Brest
Summary: Cancer therapies targeting the MAPK pathway lead to drug resistance. Overexpression of RAS has been linked to this resistance, but the underlying mechanisms are unclear. This study shows that MEK inhibitors increase translation of KRAS and NRAS oncogenes through liquid-liquid phase separation, specifically P-body dissolution. This feedback loop involving P-bodies and phase separation could be important for future targeted therapies.
Review
Oncology
Arnaud Martel, Lauris Gastaud, Christelle Bonnetaud, Sacha Nahon-Esteve, Kevin Washetine, Olivier Bordone, Myriam Salah, Virginie Tanga, Julien Fayada, Virginie Lespinet, Maryline Allegra, Salome Lalvee, Katia Zahaf, Stephanie Baillif, Corine Bertolotto, Baharia Mograbi, Sandra Lassalle, Paul Hofman
Summary: This study aims to present the challenges faced in obtaining biospecimens for research purposes in ophthalmic malignancies, and provides a detailed description of a dedicated biobank for ophthalmic malignancies. Currently, the number of biobanks dedicated to ophthalmic malignancies worldwide is very limited. The two main objectives in establishing an ophthalmic malignancy biobank are to collect scarce ophthalmic tumor samples and conduct large-scale translational research to better understand pathogenesis and discover new biomarkers for early diagnosis and targeted treatment.
Article
Health Care Sciences & Services
Veronique Hofman, Samantha Goffinet, Christophe Bontoux, Elodie Long-Mira, Sandra Lassalle, Marius Ilie, Paul Hofman
Summary: The detection of ROS1 rearrangements in metastatic non-squamous non-small cell lung carcinoma permits efficient targeted therapy. However, the rarity of ROS1 rearrangements, suboptimal specificity of ROS1 IHC, and limited availability of ROS1 FISH make the current testing algorithm challenging and time-consuming. RNA NGS can be a viable alternative to replace ROS1 IHC as a screening method.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Cell Biology
Sandy Giuliano, Christopher Montemagno, Marie-Angela Domdom, Manon Teisseire, Patrick Brest, Daniel J. Klionsky, Paul Hofman, Gilles Pages, Baharia Mograbi
Summary: Nearly fifty million older people suffer from neurodegenerative diseases, and this number is expected to triple by 2050. Defective lysosomal acidification and impairment of the autophagy pathway have been identified as modifiable risk factors for dementia. The long-term prescribing of lysosomotropic drugs to the elderly, such as proton pump inhibitors, chloroquine, and hydroxychloroquine, may be connected to the incidence of neurodegenerative diseases.
Review
Oncology
Edwin Roger Parra, Marius Ilie, Ignacio I. Wistuba, Paul Hofman
Summary: Over the past decade, there has been a revolution in cancer treatment with the shift towards targeted molecular therapies and immune-based therapies, particularly immune-checkpoint inhibitors (ICIs). However, not all patients with advanced non-small cell lung cancer (aNSCLC) benefit equally from immunotherapy, and there is a need for additional predictive markers for ICIs efficacy. Multiplex immunostaining, which evaluates multiple molecules in the tumor at the same time, shows promise in optimizing patient selection for ICIs. This review aims to explore the role of multiplex immunostaining in immuno-thoracic oncology and its current advantages and limitations in clinical practice.
BRITISH JOURNAL OF CANCER
(2023)
Review
Pathology
Paul Hofman, Sabina Berezowska, Daniel Kazdal, Baharia Mograbi, Marius Ilie, Albrecht Stenzinger, Veronique Hofman
Summary: The evolving treatment options in thoracic oncology require regular updates in diagnostic algorithms by pathologists. The pathologist plays a crucial role in deciding whether to send specimens for further analysis and can quickly obtain more material if needed. Inadequate algorithms and sampling workflow can lead to inaccurate results, impacting treatment choices and patient outcomes.
Review
Medicine, General & Internal
Durgesh Wankhede, Christophe Bontoux, Sandeep Grover, Paul Hofman
Summary: mKRAS G12C mutation has been proven to be a promising prognostic factor for patients with non-small cell lung cancer (NSCLC), negatively impacting survival. Additionally, high PD-L1 expression in these tumors opens doors for potential therapeutic opportunities.
Article
Health Care Sciences & Services
Christophe Bontoux, Aubiege Marcovich, Samantha Goffinet, Florian Pesce, Virginie Tanga, Doriane Bohly, Myriam Salah, Kevin Washetine, Zeineb Messaoudi, Jean-Marc Felix, Christelle Bonnetaud, Lihui Wang, Geetha Menon, Jean-Philippe Berthet, Charlotte Cohen, Jonathan Benzaquen, Charles-Hugo Marquette, Sandra Lassalle, Elodie Long-Mira, Veronique Hofman, Luc Xerri, Marius Ilie, Paul Hofman
Summary: We established a lymphoma biobank in Nice, France, which contains high-quality biological materials and clinical data for lymphoma patients. The biobank is ISO 9001 and ISO 20387 certified and aims to support translational research projects into lymphoma.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Arnaud Martel, Baharia Mograbi, Barnabe Romeo, Lauris Gastaud, Salome Lalvee, Katia Zahaf, Julien Fayada, Sacha Nahon-Esteve, Christelle Bonnetaud, Myriam Salah, Virginie Tanga, Stephanie Baillif, Corine Bertolotto, Sandra Lassalle, Paul Hofman
Summary: Liquid biopsy and circulating tumor cell (CTC) screening have gained attention in the past two decades for detecting various solid malignancies. However, the lack of reproducibility due to different CTC platforms using different technologies is a major limitation for daily clinical use. This study compares the efficiency of four commercially available CTC platforms for detecting and identifying uveal melanoma cells. The results show significant differences in recovery rates among the platforms, highlighting the importance of considering other factors before using a CTC platform in clinical practice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)