Article
Cell Biology
Qiuting Yan, Marisa Ninivaggi, Bas de Laat, Romy M. W. de Laat-Kremers
Summary: Thrombin generation (TG) and thrombin dynamics were assessed in platelet rich plasma (PRP) of 117 healthy individuals to establish reference values. Increasing the platelet count mainly affected the rate of prothrombin conversion and TG, rather than the total amount of thrombin formed.
Article
Food Science & Technology
Mst Shamima Parvin, Marcel Hrubsa, Jaka Fadraersada, Alejandro Carazo, Jana Karlickova, Lucie Cahlikova, Jakub Chlebek, Katerina Macakova, Premysl Mladenka
Summary: Isoquinoline alkaloids exhibit antiplatelet effects mainly at high concentrations, and bulbocapnine is a promising antiplatelet molecule.
Review
Biochemistry & Molecular Biology
Stephanie A. Renna, Steven E. McKenzie, James V. Michael
Summary: Protease-activated receptors (PARs) are membrane proteins cleaved by proteases, such as thrombin, which activate them by revealing a tethered ligand. PARs play critical roles in platelet function and are potential targets for anti-platelet therapies. While genetically modified mouse models have provided insights into platelet PAR physiology, there are limitations. This review summarizes the differences in PAR expression and function between mice and humans, and highlights new tools for studying human physiology in mouse models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Junchao Xu, Na Yan, Chunling Wang, Chao Gao, Xuexiang Han, Chengzhi Yang, Jiaqi Xu, Kun Wang, Michael J. J. Mitchell, Yinlong Zhang, Guangjun Nie
Summary: A study found that platelet-mimicking nanosponges can efficiently reverse the antiplatelet effect of drugs, reducing the risk of bleeding during surgery without causing thrombosis. This research provides a promising strategy for managing bleeding complications caused by antiplatelet therapy.
CIRCULATION RESEARCH
(2023)
Article
Hematology
Vijay K. Sonkar, Alicia S. Eustes, Azaj Ahmed, Melissa Jensen, Mitali V. Solanki, Jagadish Swamy, Rahul Kumar, Trevor P. Fidler, Jon C. D. Houtman, Bryan G. Allen, Douglas R. Spitz, E. Dale Abel, Sanjana Dayal
Summary: Reactive oxygen species (ROS) contribute to platelet hyperactivation during aging. In this study, it was found that mitochondria are an important source of platelet ROS and that mitochondrial SOD2 protects against mitochondrial ROS-driven platelet activation and thrombosis during aging.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ivan A. Muravlev, Anatoly B. Dobrovolsky, Olga A. Antonova, Svetlana G. Khaspekova, Amina K. Alieva, Dmitry V. Pevzner, Alexey V. Mazurov
Summary: Activated platelets play a role in blood coagulation by exposing phosphatidylserine (PS). Antiplatelet drugs have been found to have inhibitory effects on platelet impact in clot formation and PS exposure.
Article
Biochemical Research Methods
Chi-Ju Kim, Jungmin Kim, Jonathan Sabate del Rio, Dong Yeob Ki, Junyoung Kim, Yoon-Kyoung Cho
Summary: Platelet function tests are crucial in diagnosing inherited platelet function disorders and monitoring antiplatelet therapies. Light transmission aggregometry (LTA) is considered the gold standard, but lacks standardization and requires a large volume of blood. A centrifugal microfluidic disc has been developed to automate LTA testing from a small blood sample, producing more precise results in a shorter time.
Article
Cell Biology
Jing Yang, Claudio Mapelli, Zhaoqing Wang, Chi Shing Sum, Ji Hua, R. Michael Lawrence, Yan Ni, Dietmar A. Seiffert
Summary: Protease-activated receptor 4 (PAR4) is a promising drug target for improving the efficacy and safety of antiplatelet agents. However, the current PAR4 agonist peptides have limited utility in pharmacodynamic assays due to their high concentrations required to elicit an agonist response. This study developed a more potent PAR4 agonist peptide and a platelet aggregation assay for evaluating PAR4 antagonists, providing a valuable tool for the clinical development of PAR4 antagonists.
Article
Hematology
Philipp Mourikis, Lisa Dannenberg, Saif Zako, Carolin Helten, Rene M'Pembele, Hannah Richter, Thomas Hohlfeld, Christian Jung, Tobias Zeus, Malte Kelm, Verena Veulemans, Amin Polzin
Summary: Thrombin formation significantly increases early after TAVI, while platelet function is not affected. This may contribute to the high risk of postprocedural thromboembolic events, suggesting that extended peri-interventional anticoagulation early after TAVI may reduce the occurrence of these events.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Orthopedics
Li-Siou Chen, Chih-Kuang Chen, Jong-Hwei Su Pang, Li-Ping Lin, Tung-Yang Yu, Wen-Chung Tsai
Summary: This study investigated the effects of leukocyte-poor platelet-rich plasma (LP-PRP) and leukocyte-rich platelet-rich plasma (LR-PRP) on myoblast proliferation and gene expression. Both LP-PRP and LR-PRP promoted myoblast proliferation, increased differential gene expression, and upregulated the expression of key proteins involved in cell cycle progression. Additionally, MyoD1 expression was induced in LP-PRP and LR-PRP-treated myoblasts.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Multidisciplinary Sciences
Wenhai Cao, Xiaotong Meng, Fangming Cao, Jinpeng Wang, Maowei Yang
Summary: Diabetic non-healing wounds pose a significant burden on patients and society. Platelet-rich plasma (PRP) has been widely used for tissue regeneration and PRP-derived exosomes (PRP-Exos) have shown to be more effective in tissue regeneration. This study investigated the therapeutic potential of PRP-Exos in diabetic wound healing and found that they can promote fibroblast functions and accelerate wound healing through a specific signaling pathway.
Review
Hematology
Ronan Lordan, Alexandros Tsoupras, Ioannis Zabetakis
Summary: Platelets play a crucial role in cardiovascular diseases by modulating inflammatory pathways that impact pro-inflammatory and coagulation processes. Current antiplatelet therapies, while effective, come with an increased risk of bleeding, prompting the need for further research into safer alternative treatments.
Article
Surgery
Adil M. Lokhandwala, Samer Asmar, Muhammad Khurrum, Mohamad Chehab, Letitia Bible, Lourdes Castanon, Michael Ditillo, Bellal Joseph
Summary: This study evaluated the role of platelet transfusion on outcomes after traumatic intracranial bleeding in patients on preinjury antiplatelet agents with traumatic brain injury. Results showed that receiving two units of pooled platelets was associated with a lower risk of IB progression, neurosurgical intervention, and mortality compared to not receiving platelet transfusion.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yi-Chun Chiang, Yu-Shan Wu, Ya-Fei Kang, Hui-Chun Wang, Meng-Chun Tsai, Chin-Chung Wu
Summary: This study discovered that fully methylated resveratrol analog, TMS, can selectively inhibit PAR4-mediated platelet activation and blood coagulation at low concentrations, as well as reduce thrombus formation. These findings suggest that fully methylated resveratrol analogs with anti-PAR4 activity could be potential candidates for the development of novel antiplatelet agents.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Cell & Tissue Engineering
Shunli Rui, Yi Yuan, Chenzhen Du, Peiyang Song, Yan Chen, Hongyan Wang, Yahan Fan, David G. Armstrong, Wuquan Deng, Ling Li
Summary: Our study found that PRP activated by a mixture of calcium gluconate and thrombin harvested the highest concentration of exosomes, and had the top biological functions. This study provides a protocol for selecting appropriate PRP activators to obtain high-quality exosomes for future applications.
CELL TRANSPLANTATION
(2021)
Review
Cell Biology
A. D. Megalinskiy, V. M. Loginova, A. M. Shibeko, F. Ataullakhanov, M. A. Panteleev, D. Y. Nechipurenko
Summary: This study found that plasma coagulation under arterial shear rates can be initiated by the presence of an immobilized phospholipid fraction in the damaged vascular wall. The binding of coagulation factors to these phospholipids protects the initial stages of plasma coagulation from flow and leads to rapid thrombin production even under arterial blood shear rates.
BIOLOGICHESKIE MEMBRANY
(2022)
Article
Biology
Daria S. Morozova, Alexey A. Martyanov, Sergei Obydennyi, Julia-Jessica D. Korobkin, Alexey Sokolov, Ekaterina Shamova, Irina Gorudko, Anna L. Khoreva, Anna Shcherbina, Mikhail A. Panteleev, Anastasia N. Sveshnikova
Summary: In this study, an ex vivo experimental model for observation of granulocyte activity in thrombus formation was developed. Results showed that granulocytes from patients with WAS had reduced motility compared to healthy donors. The study suggests that WASP deficiency disrupts granulocyte behavior in thromboinflammatory conditions.
Article
Multidisciplinary Sciences
Daria Borsakova, Larisa D. Koleva, Evgeniy S. Protasov, Fazoil Ataullakhanov, Elena Sinauridze
Summary: Novel erythrocyte-bioreactors containing ALT and non-aggregating GDH were successfully produced using the flow dialysis method and the new bacterial GDH enzyme from Proteus sp. The ammonium removal rate increased linearly with an increase in encapsulated GDH activity and alanine production correlated with ammonium consumption, indicating the joint functioning of both enzymes. This suggests that the new bioreactors are promising for clinical use as they have more efficient GDH encapsulation and properties comparable to previously obtained bioreactors.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Dmitry S. Prudinnik, Elena Sinauridze, Soslan S. Shakhidzhanov, Elizaveta A. Bovt, Denis N. Protsenko, Alexander G. Rumyantsev, Fazoil Ataullakhanov
Summary: This study investigated the influence of COVID-19 on blood microrheology for the first time and identified important indicators for patient prognosis.
Article
Hematology
Alexandra A. Yakusheva, Kirill R. Butov, Georgii A. Bykov, Gabor Zavodszky, Anita Eckly, Fazly Ataullakhanov, Christian Gachet, Mikhail A. Panteleev, Pierre H. Mangin
Summary: Blood flow plays a crucial role in regulating hemostasis and arterial thrombosis. High shear rates (>3000 s(-1)) are relevant to hemostasis and not exclusive to arterial thrombosis.
Review
Cell Biology
M. A. Panteleev, A. M. Shibeko, D. Y. Nechipurenko, E. A. Beresneva, N. A. Podoplelova, A. N. Sveshnikova
Summary: Blood coagulation and fibrinolysis are controlled by scaffolds, including membranes, complexes, and polymers. These scaffolds provide special conditions for the formation and dissolution of blood clots.
BIOCHEMISTRY MOSCOW SUPPLEMENT SERIES A-MEMBRANE AND CELL BIOLOGY
(2022)
Article
Chemistry, Medicinal
Hongbo Ye, Zhifang Ma, Lei Liu, Tianci Zhang, Qiaoyi Han, Zehong Xiang, Yu Xia, Yue Ke, Xinghua Guan, Qiang Shi, Fazly Ataullakhanov, Mikhail Panteleev
Summary: In this study, nanodelivery systems based on MnO2 loaded with Ginkgolide B (GB) were designed to restore the intracerebral microenvironment in ischemic stroke. The study found that GB can protect brain tissue from oxidative damage by activating the Nrf2 signaling pathway. Meanwhile, MnO2 nanoparticles can penetrate the blood-brain barrier and enhance the delivery of therapeutic agents within brain tissue. This study provides a new therapeutic route for regulating the microenvironment of ischemic stroke.
Article
Biochemistry & Molecular Biology
Igor Mindukshev, Ekaterina Fock, Irina Dobrylko, Julia Sudnitsyna, Stepan Gambaryan, Mikhail A. Panteleev
Summary: Hypo- and hyperthermia have different effects on platelet activation and intracellular calcium concentration, leading to changes in platelet shape, aggregation, and clot formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
N. P. Novichikhina, Z. E. Ashrafova, N. Stolpovskaya, I. Ledenyova, M. G. Kholyavka, N. A. Podoplelova, M. A. Panteleev, Kh S. Shikhaliev
Summary: A series of novel compounds were obtained through a reaction, and these compounds exhibit good anticoagulant activity against blood clotting factors Xa and XIa.
RUSSIAN CHEMICAL BULLETIN
(2022)
Article
Chemistry, Organic
N. P. Novichikhina, Z. E. Ashrafova, I. Ledenyova, N. A. Podoplelova, M. A. Panteleev, Kh S. Shikhaliev
Summary: In this study, new compounds with different skeletal structures were synthesized through selective reduction reactions and their anticoagulant activity was evaluated.
RUSSIAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Anna A. Skoptsova, Alexander S. Shestakov, Irina V. Ledenyova, Nadezhda V. Stolpovskaya, Nadezhda A. Podoplelova, Mikhail A. Panteleev, Boris V. Paponov, Oleg E. Sidorenko, Khidmet S. Shikhaliev, Nadezhda P. Novichikhina
Summary: A convenient strategy for the synthesis of pyrroles lineary linked to 4H-pyrrolo[3,2,1-ij]quinolin-2(1H)-ones is presented. The cyclization of (E)-1-(2-oxo-2-phenylethylidene)-4H-pyrrolo[3,2,1-ij]quinolin-2-ones with linear and cyclic enaminones proceeds both in the presence of p-toluenesulfonic acid and triethylamine. The obtained compounds are characterised by atropoisomerism and keto-enol tautomerism. The synthesized 1-(2-phenyl-1H-pyrrol-3-y1)-4H-pyrrolo[3,2,1-ij]quinolin-2(1H)-ones were studied for anticoagulant activity.
Article
Engineering, Biomedical
Tatiana A. A. Kovalenko, Mikhail A. A. Panteleev, Anastasia N. N. Sveshnikova
Summary: Proteolytic reactions on the phospholipid membrane surface are crucial in blood clotting. Three mathematical models were constructed to investigate the impact of different complexity levels, and it was found that model C provided better predictions in certain conditions.
INTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN BIOMEDICAL ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Nadezhda P. Novichikhina, Alexander S. Shestakov, Svetlana M. Medvedeva, Anna M. Lagutina, Mikhail Yu. Krysin, Nadezhda A. Podoplelova, Mikhail A. Panteleev, Ivan S. Ilin, Alexey V. Sulimov, Anna S. Tashchilova, Vladimir B. Sulimov, Athina Geronikaki, Khidmet S. Shikhaliev
Summary: Despite extensive research, the prevention of blood clots remains an important area of study. Novel pyrroloquinolinedione-based rhodanine derivatives were synthesized and evaluated as potential molecules to inhibit clotting factors Xa and XIa. In vitro testing showed that these derivatives have the potential anticoagulant activity by inhibiting both coagulation factors Xa and XIa.
Review
Cell Biology
M. A. Panteleev, A. M. Shibeko, D. Y. Nechipurenko, E. A. Beresneva, N. A. Podoplelova, A. N. Sveshinkova
Summary: Blood coagulation and fibrinolysis systems play crucial roles in controlling the formation and dissolution of fibrin clots in blood plasma. These processes occur on specialized scaffolds, such as phospholipid membranes, fibrin and extracellular matrix protein complexes, and polymers containing phosphate groups. The physiological significance and physical meaning of these scaffolds vary, and their roles in hemostasis and thrombosis are still being studied.
BIOLOGICHESKIE MEMBRANY
(2022)
Article
Biochemistry & Molecular Biology
Kirill R. Butov, Natalia A. Karetnikova, Dmitry Y. Pershin, Dmitry Y. Trofimov, Mikhail A. Panteleev
Summary: This study showed that the presence of chromosomal mutations in the fetus affects the number and phenotype of extracellular vesicles (EVs) in amniotic fluid (AF) and leads to decreased procoagulant potency. Fetal-derived EVs were found to be the predominant source of procoagulant activity in AF.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
