Article
Chemistry, Multidisciplinary
Ryo Sasaki, Kohei Sato, Kazuhito Tabata, Hiroyuki Noji, Kazushi Kinbara
Summary: The study developed a multiblock amphiphile V-F that responds to multiple stimuli and forms a controllable supramolecular ion channel when incorporated into lipid bilayer membranes. V-F changes its molecular conformation in response to applied voltage, and its ion transport property can be reversibly switched by the addition of (R)-propranolol followed by beta-cydodextrin. This synthetic ion channel exhibits an anisotropic dual-stimuli-responsiveness for the first time.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Multidisciplinary Sciences
So Woon Kim, Mi Kyung Kim, Seokwoo Hong, Areum Choi, Jee Hye Choi, Shmuel Muallem, Insuk So, Dongki Yang, Ana Maria Cuervo, Hyun Jin Kim
Summary: This study reveals that lipid-regulated TRPML3 is the Ca2+ release channel in the phagophore, providing the necessary Ca2+ for autophagy progress. Phosphatidylinositol-3-phosphate (PI3P) is a selective regulator of TRPML3, and its interaction with TRPML3 activates the channel and promotes autophagy. These findings demonstrate that TRPML3 is a key regulator of autophagy and PI3P is crucial for its activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Edwin C. Fluck, Aysenur Torun Yazici, Tibor Rohacs, Vera Y. Moiseenkova-Bell
Summary: Transient receptor potential vanilloid 5 (TRPV5) is a calcium-selective ion channel that plays a crucial role in maintaining calcium homeostasis in the kidney. This study investigates the regulation of TRPV5 by phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) and calmodulin (CaM), as well as the effects of parathyroid hormone (PTH), metabolic acidosis, and protein kinase A (PKA) on TRPV5 activity. The findings reveal that low pH inhibits TRPV5 by preventing PI(4,5)P-2 activation, and PKA controls TRPV5 activity by preventing CaM binding and channel inactivation.
Article
Chemistry, Physical
Abdel Majid A. Adam, Moamen S. Refat
Summary: This study compared iodine CT complexes obtained from liquid and solid starting materials, finding that the solid-solid approach is faster, simpler, and does not require a purification process or consume large amounts of solvent or time. Additionally, the solid-solid approach can generate iodine CT complexes with compounds that have very low solubility in suitable organic solvents.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Review
Chemistry, Multidisciplinary
Kohei Sato, Takahiro Muraoka, Kazushi Kinbara
Summary: Transmembrane proteins, particularly ion channel proteins, are crucial for precise control over important cellular processes, and synthetic molecules mimicking their functions hold potential in sensing and manipulating biological processes. A series of multiblock amphiphiles have been developed to form supramolecular nanopores for ion transport within membranes, with the potential to respond to external stimuli.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Mi Seong Kim, Sei Hoon Yang, Min Seuk Kim
Summary: This study reveals the role of TRPML3 in regulating lysosomal exocytosis and exosomal release in the development of drug resistance in non-small cell lung cancer (NSCLC).
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Qi Chen, Hongbao Li, Hao Li, Rui Wang, Quanwei Ma, Longhai Zhang, Chaofeng Zhang
Summary: Antimony-based materials, specifically Sb nanoplates embedded in flexible N-doped carbon nanofibers, show excellent potassium storage performance with high capacity and good rate performance. The porous structure of the nanofibers enables the buffering of volume change and promotes electrolyte/ion transfer. The freestanding electrode design reduces redundancy and cost, making it suitable for wearable energy storage devices.
CHINESE CHEMICAL LETTERS
(2023)
Article
Pharmacology & Pharmacy
Agota Csoti, Rosby del Carmen Najera Meza, Ferenc Bogar, Gabor Tajti, Tibor G. Szanto, Zoltan Varga, Georgina B. Gurrola, Gabor K. Toth, Lourival D. Possani, Gyorgy Panyi
Summary: Kv1.3 K+ channels are important in regulating T cell activation and Ca2+ signaling. The novel peptide toxin sVmKTx derived from V. mexicanus smithi shows increased selectivity for Kv1.3 and potential therapeutic application in autoimmune diseases.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Garen Suna, Eda Erdemir, Lokman Liv, Simay Gunduz, Turan Ozturk, Erman Karakus
Summary: This work presents a novel probe that shows high selectivity in detecting Au(III) ions compared to other metal species. The probe offers various advantages such as naked-eye detection, fluorescence responses, low detection limit, and rapid response.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Pharmacology & Pharmacy
Wenyi Wu, Jianan Zheng, Ru Wang, Yibing Wang
Summary: This article critically evaluates the regulatory mechanisms of protein-channel interaction and post-translational modification on ion channels in metabolic diseases, and discusses the multidisciplinary methods used to elucidate these mechanisms. The analysis focuses on recently validated ion channels that regulate insulin resistance, body weight control, and adverse effects of current ion channel antagonists, providing potential new drug candidates for metabolic disorders.
BIOCHEMICAL PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Min Jeong Ji, Jeong Hee Hong
Summary: Cardiac surgeries have improved with the development of cardioplegia solutions, but challenges remain in restoring cardiac function fully. Current knowledge about cardiac electrolyte regulation through channel physiology is limited, and further research on ion channels/transporters and water channel aquaporin modulations is needed for insight into cardiac channel physiology and pathology.
Article
Multidisciplinary Sciences
Diana Hofmann, Niharika Garg, Simone Graessle, Sylvia Vanderheiden, Bruno Gideon Bergheim, Stefan Braese, Nicole Jung, Suat Oezbek
Summary: Cnidarians possess stinging organelles called nematocysts for prey capture and defense, controlled by a mechanosensory apparatus similar to vertebrate hair cells. A study identified [2.2]paracyclophanes as compounds that inhibit nematocyst discharge in Hydra, demonstrating the potential of using Hydra as a model for small molecule screens targeting mechanosensory apparatus in native tissues.
SCIENTIFIC REPORTS
(2021)
Article
Neurosciences
Iris Hardege, Julia Morud, Amy Courtney, William R. Schafer
Summary: This study identifies multiple new inhibitory ion channels with diverse ligand activation properties in Caenorhabditis elegans. These channels have inhibitory effects on acetylcholine and also show both excitatory and inhibitory effects on other major neurotransmitters. The findings shed light on the important role of diverse ligand-gated ion channels in generating complexity in a compact anatomical nervous system.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Electrochemistry
ZhangFei Su, Barbara Goodall, J. Jay Leitch, Jacek Lipkowski
Summary: The ion channel properties of gramicidin A in a model floating bilayer lipid membrane were studied, showing that the transport of Na+ across the membrane is independent of the transport of the counterion and the conductivity of the gA sodium channel depends on the transmembrane potential, with more gA molecules inserting into the bilayer as the transmembrane potential decreases.
ELECTROCHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Barbara Spix, Andrew J. Castiglioni, Natalie N. Remis, Emma N. Flores, Philipp Wartenberg, Amanda Wyatt, Ulrich Boehm, Thomas Gudermann, Martin Biel, Jaime Garcia-Anoveros, Christian Grimm
Summary: TRPML3 plays important roles in mice, including cell death in hair cells of the inner ear or skin melanocytes, survival of cochlear hair cells, neonatal enterocytes' digestion of maternal milk nutrients, expulsion of exosome-encased bacteria, and lung emphysema. TRPML3 is expressed in multiple organs and cell types, such as alveolar macrophages, melanocytes, collecting duct cells, and olfactory sensory neurons.
Article
Cell Biology
Jocelyn F. Krey, Chang Liu, Inna A. Belyantseva, Michael Bateschell, Rachel A. Dumont, Jennifer Goldsmith, Paroma Chatterjee, Rachel S. Morrill, Lev M. Fedorov, Sarah Foster, Jinkyung Kim, Alfred L. Nuttall, Sherri M. Jones, Dongseok Choi, Thomas B. Friedman, Anthony J. Ricci, Bo Zhao, Peter G. Barr-Gillespie
Summary: The stereocilia rootlet plays a critical role in protecting vertebrate hair cells from overstimulation. This study demonstrates that the ANKRD24 protein concentrates at the insertion point of the stereocilia, forming a ring and binding with TRIOBP-5 to maintain its distribution. ANKRD24 and TRIOBP-5 together organize the rootlets, enabling long-term auditory resilience.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jinkyung Kim, Anthony J. Ricci
Summary: This study tracks the entry and targeting process of a commonly used antibiotic, aminoglycosides (AGs), in the cochlea. The authors found that AGs enter the cochlea through the stria vascularis and selectively target cochlear sensory hair cells (HCs). They also identified the mechanotransducer channel and megalin as key factors in AG uptake. Blocking megalin prevented AG accumulation in HCs and reduced HC degeneration and hearing loss.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Barbara Spix, Elisabeth S. Butz, Cheng-Chang Chen, Anna Scotto Rosato, Rachel Tang, Aicha Jeridi, Veronika Kudrina, Eva Plesch, Philipp Wartenberg, Elisabeth Arlt, Daria Briukhovetska, Meshal Ansari, Gizem Gunes Gunsel, Thomas M. Conlon, Amanda Wyatt, Sandra Wetzel, Daniel Teupser, Lesca M. Holdt, Fabien Ectors, Ingrid Boekhoff, Ulrich Boehm, Jaime Garcia-Anoveros, Paul Saftig, Martin Giera, Sebastian Kobold, Herbert B. Schiller, Susanna Zierler, Thomas Gudermann, Christian Wahl-Schott, Franz Bracher, Ali Oender Yildirim, Martin Biel, Christian Grimm
Summary: The study reveals TRPML3 as a key regulator of MMP-12 clearance by alveolar macrophages, suggesting it as a therapeutic target for emphysema and chronic obstructive pulmonary disease.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Barbara Spix, Aicha Jeridi, Meshal Ansari, Ali Oender Yildirim, Herbert B. Schiller, Christian Grimm
Summary: Endolysosomal cation channels, such as TRPML3, P2X4, TRPM2, TRPV2, and TRPA1, play important roles in lung diseases such as emphysema and chronic obstructive pulmonary disease (COPD). These channels are involved in various physiological processes in the lung and understanding their functions can provide insights into lung disease.
Article
Neurosciences
Jinkyung Kim, Sriram Hemachandran, Alan G. Cheng, Anthony J. Ricci
Summary: Aminoglycosides are potent antibiotics commonly prescribed worldwide, but their use carries the risk of ototoxicity and there are currently no approved antidotes. Therefore, it is important to review recent advances in understanding aminoglycoside ototoxicity and potential intervention sites for preventing ototoxicity.
MOLECULAR AND CELLULAR NEUROSCIENCE
(2022)
Article
Medicine, Research & Experimental
Anna Scotto Rosato, Einar K. Krogsaeter, Dawid Jaslan, Carla Abrahamian, Sandro Montefusco, Chiara Soldati, Barbara Spix, Maria Teresa Pizzo, Giuseppina Grieco, Julia Boeck, Amanda Wyatt, Daniela Wuenkhaus, Marcel Passon, Marc Stieglitz, Marco Keller, Guido Hermey, Sandra Markmann, Doris Gruber-Schoffnegger, Susan Cotman, Ludger Johannes, Dennis Crusius, Ulrich Boehm, Christian Wahl-Schott, Martin Biel, Franz Bracher, Elvira De Leonibus, Elena Polishchuk, Diego L. Medina, Dominik Paquet, Christian Grimm
Summary: This study found that TPC2 activation can improve cellular phenotypes associated with LSDs, including cholesterol or lipofuscin accumulation and abnormal vacuole formation. Validation in patient cells and mouse models suggests that TPC2 is a promising target for the treatment of different types of LSDs.
EMBO MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Yu Yuan, Dawid Jaslan, Taufiq Rahman, Stephen R. Bolsover, Vikas Arige, Larry E. Wagner, Carla Abrahamian, Rachel Tang, Marco Keller, Jonas Hartmann, Anna S. Rosato, Eva-Maria Weiden, Franz Bracher, David Yule, Christian Grimm, Sandip Patel
Summary: This study reveals that TPC2 can selectively increase its permeability to calcium ions when co-activated by the endogenous ligands NAADP and PI(3,5)P-2. This finding indicates that the flux of different ions through the same channel can be independently controlled and identifies TPC2 as a potential coincidence detector that optimizes lysosomal calcium signaling.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Nouf N. Laqtom, Wentao Dong, Uche N. Medoh, Andrew L. Cangelosi, Vimisha Dharamdasani, Sze Ham Chan, Tenzin Kunchok, Caroline A. Lewis, Ivonne Heinze, Rachel Tang, Christian Grimm, An N. Dang Do, Forbes D. Porter, Alessandro Ori, David M. Sabatini, Monther Abu-Remaileh
Summary: Lysosomes play crucial roles in cellular function, and dysfunction is associated with various human diseases. The study reveals the importance of CLN3 protein in glycerophospholipid metabolism within lysosomes, as its loss leads to massive accumulation of GPDs.
Article
Biochemical Research Methods
Jinkyung Kim, Anthony J. Ricci
Summary: This article introduces a surgical procedure that allows effective imaging of cochlear cells in hearing mice, while preserving hearing thresholds. This technique may enable real-time tracking of ototoxic drug transport into the cochleae and monitoring of sound-evoked functional activity in multiple cochlear cells. It may also have implications for improving cochlear implantation in humans.
Article
Multidisciplinary Sciences
Lora L. Martucci, Jean-Marie Launay, Natsuko Kawakami, Cecile Sicard, Nathalie Desvignes, Mbarka Dakouane-Giudicelli, Barbara Spix, Maude Tetu, Franck -Olivier Gilmaire, Sloane Paulcan, Jacques Callebert, Cyrille Vaillend, Franz Bracher, Christian Grimm, Philippe Fossier, Sabine de la Porte, Hirotaka Sakamoto, John Morris, Antony Galione, Sylvie Granon, Jose-Manuel Cancela
Summary: Oxytocin is released in response to Ca2+ influx and release from intracellular stores, both critical for priming large dense-cored vesicles in hypothalamic neurons for exocytosis. This study shows that lysosomes surround dendritic vesicles and activation of endolysosomal two-pore channels (TPCs) provides the necessary Ca2+ signals to prime oxytocin release. TPC knockout mice and mice lacking TPC exhibit impaired oxytocin secretion and social behavior, which can be restored by direct administration of oxytocin.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Paroma Chatterjee, Clive P. Morgan, Jocelyn F. Krey, Connor Benson, Jennifer Goldsmith, Michael Bateschell, Anthony J. Ricci, Peter G. Barr-Gillespie
Summary: GIPC3 is initially localized to the cytoplasm of hair cells in the cochlea and then concentrated at cell junctions during postnatal development. Gipc3K & DEG;/K & DEG; mice have normal mechanotransduction currents but lack auditory brainstem response. The disruption of cell junctions and altered distribution of GIPC3 suggest that GIPC3 and MYO6 play a role in shaping the cuticular plate.
JOURNAL OF CELL SCIENCE
(2023)
Article
Neurosciences
Alexandra L. Scharr, Daibhid O. Maoileidigh, Anthony J. Ricci
Summary: The hair bundle, composed of mechanically coupled stereocilia, is a universal mechanosensory organelle in auditory, vestibular, and lateral-line systems. The dynamics of individual stereocilia in response to bundle stimulus has not been quantified. By stimulating and tracking individual inner-hair-cell stereocilia, it was found that nonuniform stimulation led to dissimilar stereociliary displacements and stereocilia closer to the stimulator moved more. The weak coupling between stereocilia suggests that more uniform stimulation across the tallest stereocilia is required to enhance the receptor current.
JOURNAL OF NEUROSCIENCE
(2023)
Meeting Abstract
Biophysics
Shefin Sam George, Thomas Effertz, Anthony J. Ricci
BIOPHYSICAL JOURNAL
(2023)
Meeting Abstract
Biophysics
Shefin Sam George, Amel El Bahloul-Jaziri, Anthony J. Ricci
BIOPHYSICAL JOURNAL
(2023)
Meeting Abstract
Biophysics
Zenghao Zhu, Anthony J. Ricci, Daibhid O. Maoileidigh
BIOPHYSICAL JOURNAL
(2022)
