Article
Immunology
Zuoji Ye, Liumei Ye, Dingbin Li, Shunsheng Lin, Wusheng Deng, Li Zhang, Jinhua Liang, Jinlong Li, Qingjun Wei, Ke Wang
Summary: In this study, the researchers found that Daphnetin (DAP) can effectively inhibit and eradicate biofilm formation of Pseudomonas aeruginosa. DAP also reduced bacterial motility and pyocyanin production. These findings suggest that DAP may serve as a natural anti-biofilm therapeutic agent.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Daria Plokarz, Michal Czopowicz, Karolina Bierowiec, Krzysztof Rypula
Summary: Pseudomonas aeruginosa is a widespread and opportunistic pathogen in dogs and cats. The ability to form biofilm and the presence of virulence factor genes contribute to the high risk of treatment complications. This study examined the prevalence of virulence factor genes involved in biofilm formation in P. aeruginosa isolates from dogs and cats. The results suggest that the fliC gene and the absence of nan1 gene could indicate the biofilm-forming ability of P. aeruginosa.
Article
Cell Biology
Dan Alexandru Toc, Alexandra Csapai, Florin Popa, Catalin Popa, Violeta Pascalau, Nicoleta Tosa, Alexandru Botan, Razvan Marian Mihaila, Carmen Anca Costache, Ioana Alina Colosi, Lia Monica Junie
Summary: This study describes a new, inexpensive, and easy to reproduce protocol for a 3D-printed microfluidic device for studying bacterial biofilm formation. The results obtained from the experiments show different characteristics of biofilm production between Enterococcus faecalis and Pseudomonas aeruginosa.
Article
Food Science & Technology
Qingli Dong, Linjun Sun, Taisong Fang, Yuan Wang, Zhuosi Li, Xiang Wang, Mengjie Wu, Hongzhi Zhang
Summary: This study investigated the mono- and dual-species biofilm formation of L. monocytogenes and P. aeruginosa under different culture conditions. Results showed the inhibitory effect of chicken juice on L. monocytogenes biofilm formation, as well as the dominance of P. aeruginosa in dual-species biofilms in certain culture mediums. The correlation between motility and biofilm formation was found to be insignificant.
Article
Microbiology
Esther Shmidov, Ilana Lebenthal-Loinger, Shira Roth, Sarit Karako-Lampert, Itzhak Zander, Sivan Shoshani, Amos Danielli, Ehud Banin
Summary: The functions of bacterial TA systems are still disputed and poorly understood. This study sheds light on potential functions of bacterial TA systems. The novel type II TA system PrrT/A in Pseudomonas aeruginosa is found to regulate biofilm formation, motility, prophage induction, bacteriophage production, and aminoglycoside resistance, providing new insights into the diverse roles and impacts of TA systems in bacteria.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Ruan Fourie, Jacobus Albertyn, Olihile Sebolai, Onele Gcilitshana, Carolina H. Pohl
Summary: The interaction between Candida albicans and Pseudomonas aeruginosa affects early biofilm formation and virulence of C. albicans in an infection model. Deletion of SET3 does not affect C. albicans morphology in the presence of P. aeruginosa, but it reduces virulence in a Caenorhabditis elegans infection model.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Mario Gajdacs, Zoltan Barath, Krisztina Karpati, Dora Szabo, Donatella Usai, Stefania Zanetti, Matthew Gavino Donadu
Summary: The study found a relationship between biofilm formation and expression of virulence factors with multidrug resistance in Pseudomonas aeruginosa. However, there were no significant differences in virulence factor expression between multidrug-resistant and non-multidrug-resistant isolates, and no correlations were seen between the rate of biofilm formation, pigment production, or motility. Understanding the interplay between drug resistance mechanisms, biofilm formation, and virulence is crucial for managing chronic bacterial infections.
Article
Biotechnology & Applied Microbiology
Yushan Xia, Congjuan Xu, Dan Wang, Yuding Weng, Yongxin Jin, Fang Bai, Zhihui Cheng, Oscar P. Kuipers, Weihui Wu
Summary: YbeY is a highly conserved RNase in bacteria that plays essential roles in rRNA processing, small RNA regulation, and bacterial responses to environmental stresses. Mutation of the ybeY gene in Pseudomonas aeruginosa leads to dysregulation of secretion system gene expression and biofilm formation, indicating the pleiotropic functions of YbeY in bacterial regulation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Telma de Sousa, Michel Hebraud, Olimpia Alves, Eliana Costa, Luis Maltez, Jose Eduardo Pereira, Angela Martins, Gilberto Igrejas, Patricia Poeta
Summary: A study conducted at the Medical Center of Tras-os-Montes and Alto Douro (CHTMAD) found that Pseudomonas aeruginosa strains causing urinary tract infections are resistant to multiple antibiotics and have high biofilm formation and motility capabilities, posing a serious public health problem.
Article
Immunology
Wusheng Deng, Yanmei Lei, Xiujia Tang, Dingbin Li, Jinhua Liang, Jing Luo, Liuyuan Liu, Wenshu Zhang, Liumei Ye, Jinliang Kong, Ke Wang, Zhaoyan Chen
Summary: This study investigated whether DNase I can inhibit the early formation of biofilms in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models. The results showed that DNase I significantly inhibited early biofilm formation in a dose-dependent manner, without affecting bacterial growth. Therefore, DNase I has therapeutic potential against empyema biofilms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Ali Hazim Abdulkareem, Anmar Kamil Alalwani, Mohammed Mukhles Ahmed, Safaa Abed Latef Al-Meani, Mohammed Salih Al-Janaby, Al-Moghira Khairi Al-Qaysi, Ali Ibrahim Edan, Hasan Falah Lahij
Summary: The increasing disparity between antimicrobial resistance and the development of new antimicrobials continues to pose a significant global health concern. This study found that goldenrod extract has the ability to inhibit the production of extended-spectrum beta-lactamases (ESBLs) and biofilm formation in ESBL-Pseudomonas aeruginosa.
Article
Multidisciplinary Sciences
Eleonora Secchi, Giovanni Savorana, Alessandra Vitale, Leo Eberl, Roman Stocker, Roberto Rusconi
Summary: It has been found that the mechanical stability of Pseudomonas aeruginosa streamers mainly relies on the presence of extracellular DNA (eDNA) and the exopolysaccharide Pel. Controlling the composition of streamers can effectively control the formation of biofilm structures.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Dan Yang, Suqi Hao, Ling Zhao, Fei Shi, Gang Ye, Yuanfeng Zou, Xu Song, Lixia Li, Zhongqiong Yin, Xiaoli He, Shiling Feng, Helin Chen, Yu Zhang, Yuanze Gao, Yinglun Li, Huaqiao Tang
Summary: The study found that paeonol has good anti-biofilm activity against Gram-negative bacteria and anti-QS activity, effectively reducing the virulence of P. aeruginosa. In vivo studies showed that paeonol could reduce the pathogenicity of P. aeruginosa and enhance the survival rate of C. elegans.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Taehyeong Lim, So-Young Ham, SangJin Nam, Myoungsun Kim, Ki Yong Lee, Hee-Deung Park, Youngjoo Byun
Summary: This article reviews recent studies on small molecules targeting the RhlI/RhlR system in P. aeruginosa, discussing their in vitro and in vivo biological activities. The article focuses on structure-activity relationship studies and the outlook for next-generation anti-biofilm agents.
Article
Chemistry, Multidisciplinary
Xiaodie Yuan, Xia Zhao, Yuan Lin, Zhaohui Su
Summary: This study synthesized PDA nanoparticles of various sizes and introduced different surface groups to investigate their penetration and accumulation abilities in biofilms. The PDA nanoparticles of approximately 60 nm size showed higher penetration and accumulation abilities, and the positively charged amine groups were more effective in enhancing the interactions with the biofilm compared to carboxyl or methoxy groups. The PDA60 nanoparticles with amine surface groups also exhibited good photothermal properties.
Article
Orthopedics
Raman Mundi, Harman Chaudhry, Seper Ekhtiari, Prabjit Ajrawat, Daniel M. Tushinski, Thomas J. Wood, Mohit Bhandari
Summary: This study conducted a systematic review and meta-analysis to assess the impact of hydrofiber dressings on reducing complications in total joint arthroplasty surgeries. The results showed that hydrofiber dressings had no observable effect on prosthetic joint infection and wound irritation, but they did reduce the rate of blisters and the number of dressing changes. However, the evidence for the reduction in blisters and dressing changes is limited due to biased trial methodologies and wide confidence intervals.
Article
Ophthalmology
Thomas Charles Wood, Sundas Maqsood, Alex Saunders, William Sancha, Mayank A. Nanavaty, Michael Wearne, Saul Rajak
Summary: The objective of this study was to develop and implement a simulation-based training model for the management of posterior capsule rupture (PCR) from a non-technical skills (NTS) perspective. The results showed significant improvement in participants' NTS and non-significant improvement in their technical skills (TS).
Article
Evolutionary Biology
Silas Bossert, Thomas J. Wood, Sebastien Patiny, Denis Michez, Eduardo A. B. Almeida, Robert L. Minckley, Laurence Packer, John L. Neff, Robert S. Copeland, Jakub Straka, Alain Pauly, Terry Griswold, Sean G. Brady, Bryan N. Danforth, Elizabeth A. Murray
Summary: The mining bees (Andrenidae) are a major bee family with over 3000 species globally. Despite limited knowledge of their evolutionary history, a comprehensive genomic dataset of 195 species has revealed that their diversification rates sharply increased in the past 15 million years, especially in the genera Andrena and Perdita. This suggests that these two groups, along with the brood parasites of the genus Nomada Scopoli, are among the fastest diversifying lineages of all bees.
SYSTEMATIC ENTOMOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Thomas R. Wood, Kate Hildahl, Hawley Helmbrecht, Kylie A. Corry, Daniel H. Moralejo, Sarah E. Kolnik, Katherine E. Prater, Sandra E. Juul, Elizabeth Nance
Summary: Organotypic brain slice models are used to investigate therapeutic options for hypoxic-ischemic brain injury, a major factor in neonatal mortality. The study found highly heterogeneous regional responses to different treatments and different morphologies of microglia responded differently to injury and treatment.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Medicine, General & Internal
Sandra E. Juul, Thomas R. Wood, Bryan A. Comstock, Krystle Perez, Semsa Gogcu, Mihai Puia-Dumitrescu, Sara Berkelhamer, Patrick J. Heagerty
Summary: Understanding the causes and circumstances of death in extremely preterm infants is crucial for healthcare practitioners. This study retrospectively examined a cohort of infants born between 24 and 27 weeks of gestation to determine the risk factors, causes, timing, and circumstances of death. The study found that respiratory distress or failure, pulmonary hemorrhage, necrotizing enterocolitis, catastrophic intracranial hemorrhage, sepsis, and sudden unexplained death were the primary causes of death. The risk of death decreased over time, and preterm labor was associated with a decreased hazard of death. Clinical factors such as low birth weight, low Apgar score, sick appearance at birth, necrotizing enterocolitis, pulmonary hemorrhage, severe intracranial hemorrhage, and severe sepsis were also associated with death.
Article
Agriculture, Multidisciplinary
N. Leclercq, L. Marshall, T. Weekers, A. Anselmo, D. Benda, D. Bevk, P. Bogusch, D. Cejas, B. Drepper, M. Galloni, M. Gerard, G. Ghisbain, L. Hutchinson, B. Martinet, D. Michez, J-M Molenberg, P. Nikolic, S. Roberts, G. Smagghe, J. Straka, P. Vandamme, T. J. Wood, N. J. Vereecken
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2022)
Article
Biodiversity Conservation
Timothy Weekers, Leon Marshall, Nicolas Leclercq, Thomas J. Wood, Diego Cejas, Bianca Drepper, Michael Garratt, Louise Hutchinson, Stuart Roberts, Jordi Bosch, Laura Roquer-Beni, Patrick Lhomme, Denis Michez, Jean-Marc Molenberg, Guy Smagghe, Peter Vandamme, Nicolas J. Vereecken
Summary: This study investigates the impact of variables such as climate, landscape composition, management practices, and wild bee pollination on commercial apple production. The results show that apple yields are mainly influenced by management practices, not the contribution of managed honey bees. Wild bee diversity is negatively correlated with honey bee dominance but promotes apple quality. Thus, harnessing wild bee diversity can be a nature-based solution and a substitute for an exclusive reliance on honey bees in apple production.
ECOLOGICAL INDICATORS
(2022)
Article
Ophthalmology
Thomas Charles Wood, Sundas Maqsood, William Sancha, Alex Saunders, David Lockington, Mayank A. Nanavaty, Saul Rajak
Editorial Material
Microbiology
Joy M. Muthami, Laura Fernandez-Garcia, Maria Tomas, Thomas K. Wood
ENVIRONMENTAL MICROBIOLOGY
(2022)
Editorial Material
Environmental Sciences
Sooyeon Song, Thomas K. Wood
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Agriculture, Multidisciplinary
R. N. Nichols, T. J. Wood, J. M. Holland, D. Goulson
Summary: The floral abundance and richness on farmland have been decreasing since the mid 1900s. Agri-environment schemes (AES) can help improve floral resources for insect pollinators through establishing flower-rich areas or carefully managing areas set-aside for natural regeneration. A study conducted in southern England showed that initially the sown Pollen & Nectar Strips and Florally Enhanced (FE) Grass Margins provided the greatest floral abundance, but this declined over time. Furthermore, only a few sown species known to be beneficial for insect pollinators persisted in the long term.
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Sooyeon Song, Ekaterina Semenova, Konstantin Severinov, Laura Fernandez-Garcia, Michael J. Benedik, Toshinari Maeda, Thomas K. Wood
Summary: This study reveals the activity of the CRISPR-Cas system in Escherichia coli and its role in inhibiting cryptic prophages through RNA interference. These findings provide new insights into the function of the CRISPR-Cas system in bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Laura Fernandez-Garcia, Thomas K. Wood
Summary: The overlap between phage-defense systems (PDs) and toxin/antitoxin systems (TAs) has become clear as new PDs are discovered, as both systems use similar means to reduce cellular metabolism. They both have members that deplete energetic compounds and nucleic acids, and inflict membrane damage. Additionally, both systems reduce host metabolism to limit phage propagation and interaction of multiple defense systems.
Article
Microbiology
Laura Fernandez-Garcia, Sooyeon Song, Joy Kirigo, Michael E. Battisti, Maiken E. Petersen, Maria Tomas, Thomas K. Wood
Summary: This study reveals that bacteria can defend against phage infection by forming persister cells instead of inducing cell suicide. Furthermore, the restriction/modification systems work together with the toxin/antitoxin system to clear phage DNA. These findings are crucial for the success of phage therapy.
MICROBIOLOGY SPECTRUM
(2023)
Article
Green & Sustainable Science & Technology
Thomas K. Wood, Ilke Gurgan, Ethan T. Howley, Ingmar H. Riedel-Kruse
Summary: The anaerobic oxidation of methane (AOM) in microbial fuel cells (MFCs) shows great promise in converting methane into electricity and value-added chemicals. By integrating different technologies, the performance of AOM MFCs can be significantly improved, leading to sustainable energy production.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2023)