期刊
PLOS ONE
卷 8, 期 1, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0053816
关键词
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资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP [2008/10254-1, 2009/53637-0]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq/Departamento de Ciencia e Tecnologia - DECIT [555648/2009-5]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - CAPES
- Instituto Nacional de Ciencia e Tecnologia em Entomologia Molecular - INCT-EM
Aedes aegypti, the main vector of dengue virus, requires a blood meal to produce eggs. Although live animals are still the main blood source for laboratory colonies, many artificial feeders are available. These feeders are also the best method for experimental oral infection of Ae. aegypti with Dengue viruses. However, most of them are expensive or laborious to construct. Based on principle of Rutledge-type feeder, a conventional conical tube, glycerol and Parafilm-M were used to develop a simple in-house feeder device. The blood feeding efficiency of this apparatus was compared to a live blood source, mice, and no significant differences (p = 0.1189) were observed between artificial-fed (51.3% of engorgement) and mice-fed groups (40.6%). Thus, an easy to assemble and cost-effective artificial feeder, designated Glytube was developed in this report. This simple and efficient feeding device can be built with common laboratory materials for research on Ae. aegypti.
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