Article
Virology
Dayvson Mauricio da Silva, Juliana Prado Goncales, Jose Valter Joaquim Silva Junior, Thaisa Regina Rocha Lopes, Luan Araujo Bezerra, Virginia Maria Barros de Lorena, Maria Rosangela Cunha Duarte Coelho
Summary: In HIV/HHV-8 coinfection, levels of cytokines IL-4, IL-6, and IL-10 are higher in patients compared to those infected with only HIV, potentially indicating the development of HHV-8-related diseases. On the other hand, levels of IL-2, TNF-alpha, and IFN-gamma show no significant difference between the two groups.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Xiaoyang Ma, Yoon-Jung Shin, Hyo-Min Jang, Min-Kyung Joo, Jong-Wook Yoo, Dong-Hyun Kim
Summary: The study showed that human fecal Lactobacillus rhamnosus NK210 and Bifidobacterium longum NK219 have positive effects on immune homeostasis, particularly in controlling immune imbalance and gut dysbiosis.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Felix Clemens Richter, Matthias Friedrich, Nadja Kampschulte, Klara Piletic, Ghada Alsaleh, Ramona Zummach, Julia Hecker, Mathilde Pohin, Nicholas Ilott, Irina Guschina, Sarah Karin Wideman, Errin Johnson, Mariana Borsa, Paula Hahn, Christophe Morriseau, Bruce D. Hammock, Henk Simon Schipper, Claire M. Edwards, Rudolf Zechner, Britta Siegmund, Carl Weidinger, Nils Helge Schebb, Fiona Powrie, Anna Katharina Simon
Summary: Lipids play a major role in inflammatory diseases through altering inflammatory cell functions. Autophagy, a lysosomal degradation pathway, is found to regulate lipid availability, which affects inflammation. Loss of autophagy gene Atg7 in adipocytes exacerbates intestinal inflammation by causing an imbalance of oxylipins, reducing IL-10 secretion, and exacerbating intestinal inflammation through the cytochrome P450-EPHX pathway.
Article
Immunology
Bazgha Gul, Rukhsana Anwar, Mohammad Saleem, Afifa Noor, Muhammad Ihsan Ullah
Summary: The current study evaluated the anti-arthritic activity of n-hexane and aqueous extracts of Cassia absus seeds. The extracts showed significant inhibition of paw edema and reduction in paw size and joint diameter. The extracts also improved the levels of inflammatory and oxidative biomarkers. Polymerase chain reaction investigation revealed the modulation of various inflammatory factors by the extracts. In conclusion, Cassia absus can effectively alleviate arthritis symptoms by modulating oxidative and inflammatory biomarkers.
INFLAMMOPHARMACOLOGY
(2023)
Article
Nutrition & Dietetics
Catarina Durao, Milton Severo, Andreia Oliveira, Carla Lopes
Summary: This study evaluated the association between dietary patterns at 4 years old and adiposity and cardiometabolic risk factors at 10 years old, finding that a high energy-dense food (EDF) dietary pattern at 4 years old was significantly associated with obesity, insulin levels, and HOMA-IR in girls.
Article
Cell Biology
Ana Cardoso, Ana Catarina Martins, Ana Raquel Maceiras, Wei Liu, Isabel Castro, Antonio G. Castro, Antonio Bandeira, James P. Di Santo, Ana Cumano, Yan Li, Paulo Vieira, Margarida Saraiva
Summary: The study reveals that IL-10 triggers emergency myelopoiesis (EM) by expanding the myeloid progenitor compartment and production of myeloid cells. Unexpectedly, IL-10 also reprograms CD4 and CD8 T cells to produce IFN-gamma, suggesting new perspectives for IL-10-based immunotherapies.
Article
Medicine, General & Internal
Taosheng Ye, Jiaohong Zhang, Xuan Zeng, Yuxiang Xu, Min Li
Summary: This study found that levels of IFN-gamma, IL-10, IL-12, and CCL1 in the serum of patients with pulmonary tuberculosis are significantly increased, indicating immune impairment. After treatment, these levels decreased and were found to be correlated with other inflammatory markers.
ACTA MEDICA MEDITERRANEA
(2021)
Article
Immunology
Julio Flores-Gonzalez, Alexia Urban-Solano, Lucero A. Ramon-Luing, Juan Carlos Cancino-Diaz, Araceli Contreras-Rodriguez, Everardo Curiel-Quesada, Rogelio Hernandez-Pando, Leslie Chavez-Galan
Summary: This study evaluated the distribution of circulating B cell subsets in tuberculosis (TB) patients and their response to stimuli from M.tb. The results showed that patients with different types of TB have altered frequencies of circulating B cells. Drug-sensitive and drug-resistant TB patients displayed unique characteristics and produced different levels of cytokines.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Nutrition & Dietetics
Ju Hee Kim, Seung Won Lee, Jung Eun Lee, Eun Kyo Ha, Man Yong Han, Eun Lee
Summary: Breastfeeding in early infancy is associated with reduced risks of various childhood diseases, hospitalization, obesity, and short stature. Exclusive breastfeeding at 4-6 months has similar effects to exclusive breastfeeding over 6 months.
Article
Biochemistry & Molecular Biology
Mengdie Li, Fengrun Sun, Jinfeng Qian, Lanting Chen, Dajin Li, Songcun Wang, Meirong Du
Summary: Establishing maternal-fetal tolerance and successful placentation are crucial for a successful pregnancy, as disruption of immune balance and inadequate placental perfusion can lead to pregnancy-related complications. Extravillous trophoblasts play a key role in regulating decidual immune cells for fetal tolerance. Blocking Tim-3 and CTLA-4 pathways can lead to abnormal interaction between decidual immune cells and extravillous trophoblasts, resulting in poor placental development and increased fetal loss. IL-4 and IL-10 treatment may rescue the adverse effects of targeting Tim-3 and CTLA-4 on pregnancy outcomes, suggesting them as potential therapeutic strategies to prevent pregnancy loss induced by checkpoint inhibition.
Article
Immunology
Xiaolei Wu, Qi Shen, Haocai Chang, Junyu Li, Da Xing
Summary: This study demonstrated that photobiomodulation therapy (PBMT) effectively promotes adult hippocampal neurogenesis (AHN) and reverses cognitive deficits in AD mouse models by upregulating expression of interferon-gamma (IFN-γ) and interleukin-10 (IL-10) in non-parenchymal CD4(+) T cells through the JAK2/STAT4/STAT5 signaling pathway.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Dermatology
Beatriz Oliveira Fagundes, Thamires Rodrigues de-Sousa, Jefferson Russo Victor
Summary: Atopic dermatitis (AD) is a chronic disease that typically starts in childhood and can persist into adulthood. The major cytokines involved in inflammatory skin diseases, including AD, are IL-4, IL-17, IFN-gamma, and IL-10. The role of gamma delta T cells in AD is still largely unexplored.
INTERNATIONAL JOURNAL OF DERMATOLOGY
(2023)
Article
Immunology
Qiuhua Zhu, Huan Li, Shanshan Zheng, Bin Wang, Mingjie Li, Wenbin Zeng, Lanlan Zhou, Zebing Guan, Hong Wang, Yanan Liu, Yanmin Gao, Shiqiu Qiu, Chaolun Chen, Shimei Yang, Yuemei Yuan, Hanling Zhang, Guanqiao Ruan, Xueyi Pan
Summary: This study investigates the Th1/Th2 cytokine profile in patients with lymphoma during the myelosuppression stage of infection and proposes a rapid diagnostic method to determine the type of infection and anti-infective effect. IL-6 and IL-10 are closely associated with the severity and treatment efficacy in lymphoma patients who develop infections after chemotherapy, and can help distinguish between Gram-negative and Gram-positive bacterial infections at an early stage.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Kaveh Abdi, Karen Laky, Mehrnoosh Abshari, Elizabeth M. Hill, Larry Lantz, Nevil J. Singh, Eric O. Long
Summary: It has been found that NK cells produce IFN-gamma before the production of IL-12 by DCs in the early stage of immune response. Toll-like Receptor 4 (TLR4) on DCs and soluble factors produced by LPS-activated DCs play important roles in IFN-gamma production by NK cells.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Zixi Wang, Di Guan, Jianxin Huo, Subhra K. Biswas, Yuhan Huang, Yuansheng Yang, Shengli Xu, Kong-Peng Lam
Summary: The study demonstrates that IL-10 upregulates both glycolysis and oxidative phosphorylation in human NK cells, crucial for their enhanced effector functions. Mechanistically, it is revealed that IL-10 activates the mammalian target of rapamycin complex 1 (mTORC1) to regulate metabolic reprogramming in human NK cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Rheumatology
Margarita Ivanchenko, Gudny Ella Thorlacius, Malin Hedlund, Vijole Ottosson, Lauro Meneghel, Sophia Bjorkander, Amina Ossoinak, Joanna Tingstrom, Katarina Bremme, Eva Sverremark-Ekstrom, Kristina Gemzell-Danielsson, Sven-Erik Sonesson, Karine Chemin, Marie Wahren-Herlenius
Summary: This study reveals altered immune cell profiles in anti-Ro/La+ mothers and neonates, with increased NK cell frequencies in exposed neonates. The presence of type I and II interferon footprints and upregulation of NK cell activating ligands in fetal cardiac cells after exposure to type I interferon suggest innate immune activation in neonates of anti-Ro/La+pregnancy, potentially contributing to the risk of CHB.
ANNALS OF THE RHEUMATIC DISEASES
(2021)
Article
Gastroenterology & Hepatology
Yuying Liu, Thomas K. Hoang, Christopher M. Taylor, Evelyn S. Park, Jasmin Freeborn, Meng Luo, Stefan Roos, J. Marc Rhoads
Summary: In the treatment of Treg-deficiency-induced autoimmunity, Limosilactobacillus reuteri DSM 17938 (LR) showed greater efficacy than Lacticaseibacillus rhamnosus GG (LGG). The study demonstrated that two different probiotics produce distinct signatures in the fecal microbial community in mice with Treg deficiency, but with many similarities in global plasma metabolites in general. However, there are strain-specific microbial products with different anti-inflammatory properties, reinforcing the concept that one size does not fit all in the treatment of autoimmune disease.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hao Luo, Peishun Li, Hao Wang, Stefan Roos, Boyang Ji, Jens Nielsen
Summary: The metabolic features and diversities of Limosilactobacillus reuteri strains were systematically investigated using genome-scale metabolic models (GEMs). The reconstructed GEM of L. reuteri ATCC PTA 6475 contains 894 reactions and 726 metabolites linked to 622 metabolic genes, which can simulate growth and amino acids utilization. Comparison of GEMs for 35 L. reuteri strains identified potential metabolic products associated with host adaptation.
Article
Cell Biology
Manuel Mata Forsberg, Claudia Arasa, Willemien van Zwol, Sibel Uzuncayir, Anna Schonbichler, Paulina Regenthal, Jenny Schelin, Karin Lindkvist-Petersson, Sophia Bjorkander, Eva Sverremark-Ekstrom
Summary: Staphylococcal enterotoxins (SE) trigger the activation of MAIT, gamma delta T, and NK cells mainly by reducing IFN-gamma expression through neutralizing IL-12, while cytotoxicity remains unaffected. Responses of unconventional T cells and NK cells occur subsequent to conventional T cells, and SEA's ability to activate gamma delta T and NK cells is dependent on the presence of monocytes and alpha beta T cells. Additionally, SE induces a diminished and delayed cytokine response in infants, particularly within unconventional T and NK cell populations.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Microbiology
Hao-Yu Liu, Antoine Giraud, Cedric Seignez, David Ahl, Feilong Guo, John Sedin, Tomas Walden, Jee-Hwan Oh, Jan Peter van Pijkeren, Lena Holm, Stefan Roos, Stefan Bertilsson, Mia Phillipson
Summary: The study shows that oral administration of the probiotic bacterium Limosilactobacillus reuteri R2LC increases the numbers and effector functions of B cell subsets, leading to enhanced IgA production, altered intestinal microbiota, and protection against inflammation.
Article
Pharmacology & Pharmacy
Emelie Ohnstedt, Hava Lofton Tomenius, Peter Frank, Stefan Roos, Evelina Vagesjo, Mia Phillipson
Summary: Non-healing wounds are a growing medical problem that causes significant suffering. In this study, the topical administration of the drug candidate ILP100 proved to be safe and accelerated wound healing in minipigs, using different evaluation methods.
Article
Gastroenterology & Hepatology
Gintare Lasaviciute, Myriam Barz, Marieke van der Heiden, Claudia Arasa, Kanwal Tariq, Jaclyn Quin, Ann-Kristin Ostlund Farrants, Eva Sverremark-Ekstrom
Summary: The secretome of the gut probiotic bacterium L. reuteri has an effect on memory responses in human immune cells, resulting in a secondary response phenotype different from other training agents. This has potential implications for therapeutic interventions targeting T cell responses.
Article
Immunology
Isabella Badolati, Marieke van der Heiden, David Brodin, Marit Zuurveld, Szilvia Szilagyi, Sophia Bjorkander, Eva Sverremark-Ekstrom
Summary: In this study, it was found that Staphylococcus aureus enhances Th9 cell frequency and IL-9 secretion, which may contribute to the development of allergic diseases. Additionally, retinoic acid can suppress the Th9 responses induced by S. aureus and alter the gene expression associated with allergic inflammation. These findings highlight the importance of microbial and dietary factors in modulating Th9 cell polarization.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Microbiology
Yanhong Pang, Ludwig Ermann Lundberg, Manuel Mata Forsberg, David Ahl, Helena Bysell, Anton Pallin, Eva Sverremark-Ekstroem, Roger Karlsson, Hans Jonsson, Stefan Roos
Summary: Bacterial extracellular membrane vesicles (MV) are important mediators of microbe-host signals in host-pathogen interactions and interactions between mutualistic bacteria and their hosts. The study focused on MV derived from probiotics and found that they carry numerous bacterial cell surface proteins, some of which are involved in host-bacteria interactions. They can be internalized by epithelial cells, decrease leakage caused by enterotoxigenic Escherichia coli, regulate inflammatory responses in immune cells, and antagonize pain receptor channels. The findings have implications for the development of innovative and more efficient probiotic products.
FRONTIERS IN MICROBIOLOGY
(2022)
Letter
Allergy
Carina Uhl, Eva Sverremark-Ekstrom, Magnus Borres, Motohiro Ebisawa, Erik Melen, Mika J. Makela, Jon R. Konradsen, Brian P. Vickery, Anna Asarnoj, Caroline Nilsson
CLINICAL AND EXPERIMENTAL ALLERGY
(2023)
Article
Microbiology
Irene Vitale, Mattia Spano, Valentina Puca, Simone Carradori, Stefania Cesa, Beatrice Marinacci, Francesca Sisto, Stefan Roos, Gianfranco Grompone, Rossella Grande
Summary: This study evaluated the antibiofilm activity of cell-free supernatant (CFS) and its sub-fractions (SurE 10 K and SurE) produced by Limosilactobacillus reuteri DSM 17938 against biofilm-producing bacterial species. The results showed that CFS exhibited promising antibiofilm activity against biofilms formed by clinically relevant microorganisms. NMR metabolomic analysis identified and quantified several compounds, mainly organic acids and amino acids, with lactate being the most abundant metabolite in all samples. The storage stability of these postbiotics was evaluated using the CIEL*a*b parameters.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Emelie Ohnstedt, Evelina Vagesjo, Andreas Fasth, Hava Lofton Tomenius, Pia Dahg, Sofia Jonsson, Nisha Tyagi, Mikael Astrom, Zhanar Myktybekova, Lovisa Ringstad, Margareth Jorvid, Peter Frank, Per Heden, Stefan Roos, Mia Phillipson
Summary: This study aimed to evaluate the safety and tolerability of ILP100-Topical drug in humans and investigate its clinical and biological effects on wound healing. The results showed that ILP100-Topical was safe and well-tolerated in all individuals and doses, and it accelerated wound healing and increased the density of CXCL12+ cells in the wounds.
Article
Biotechnology & Applied Microbiology
Peishun Li, Stefan Roos, Hao Luo, Boyang Ji, Jens Nielsen
Summary: Many studies have shown the link between gut microbiota and human health and disease. Manipulation of gut microbiota, such as supplementation of probiotics, has been suggested as a feasible method, but with limited therapeutic efficacy. In order to develop effective microbiota-targeted diagnostic and therapeutic strategies, metabolic engineering has been applied to construct genetically modified probiotics and synthetic microbial consortia.
METABOLIC ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Malin Sendelius, Jakob Axelsson, Peidi Liu, Stefan Roos
Summary: This study conducted a systematic safety assessment of Limosilactobacillus reuteri ATCC PTA 4659 through genome analysis, phenotypic characterization, and a human clinical safety study. The results showed that the strain is susceptible to antibiotics and does not possess virulence or antibiotic resistance genes. It was also found to have a high adhesion to mucus and tolerance to gastric pH and bile, indicating its potential as a probiotic strain. The human clinical safety study demonstrated that the strain is safe for human consumption.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2023)