Article
Microbiology
Jesus Borrego, Muhammad Umair Naseem, Al Nasar Ahmed Sehgal, Lipsa Rani Panda, Kashmala Shakeel, Attila Gaspar, Cynthia Nagy, Zoltan Varga, Gyorgy Panyi
Summary: The Kv1.3 channel is a therapeutic target for various diseases. The Pichia expression system allows for efficient production of Kv1.3 channel blockers from scorpion venom, providing a cost-effective approach for their characterization and potential therapeutic application.
Article
Pharmacology & Pharmacy
Muhammad Umair Naseem, Gabor Tajti, Attila Gaspar, Tibor G. Szanto, Jesus Borrego, Gyorgy Panyi
Summary: The recombinant expression of MgTx in Pichia pastoris resulted in a significantly higher yield of correctly folded peptides, which were able to block specific potassium channels effectively. The presence of a His-tag did not affect the blocking properties of the toxin, making recombinant toxins a cost-effective option for ion channel research.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zuzana Rosenbergova, Kristina Kantorova, Martin Simkovic, Albert Breier, Martin Rebros
Summary: The study successfully upscaled the production of Myrosinase to a 3 L laboratory fermenter, optimizing fermentation conditions resulted in a threefold increase in Myrosinase productivity and 1.5-fold increase in dry cell weight.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Zuzana Rosenbergova, Zuzana Hegyi, Miroslav Ferko, Natalia Andelova, Martin Rebros
Summary: The deletion of a native signal sequence in plants significantly increased the production of myrosinase in Pichia pastoris, leading to changes in enzyme properties. These findings could provide insights for the expression of other plant enzymes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Maria Carmela Bonaccorsi di Patti, Antimo Cutone, Zuzana Pakanova, Marek Nemcovic, Peter Barath, Giovanni Musci
Summary: Ceruloplasmin is crucial for iron metabolism in vertebrates, and the production of high-quality recombinant human ceruloplasmin represents a significant advancement. Further improvement in productivity and glycoengineering is necessary for potential therapeutic use in aceruloplasminemia patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Jewel Ann Joseph, Simen Akkermans, Jan F. M. Van Impe
Summary: The sweet protein thaumatin is a promising sugar replacer, and its large-scale extraction from natural plants can be limited in terms of raw material availability and production costs. This study evaluated the impact of pH and temperature on the production of thaumatin II protein using Pichia pastoris, and found that a pH of 6.0 and a temperature of 30 degrees C resulted in the highest protein levels.
Article
Biotechnology & Applied Microbiology
Xingxing Liang, Hong Jiang, Xiandong Si, Qi Xin, Di Meng, Peng Chen, Xiangzhao Mao
Summary: The study successfully increased the expression level of plectasin in recombinant Pichia pastoris by constructing a four-copy cassette of plectasin via 2A peptide assembly. This strategy based on 2A peptide assembly can enhance the expression of plectasin and further expand its application prospect.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Fidan Erden-Karaoglan, Mert Karaoglan
Summary: This study investigated the applicability of heterologous yeast promoters in Pichia pastoris, and found inducible and constitutive regulation modes as well as the optimal carbon sources for these promoters. The study also determined the DNA region with the highest activity for all promoters through promoter deletion analysis.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Microbiology
Yingjie Pan, Jiao Yang, Jianping Wu, Lirong Yang, Hao Fang
Summary: This article summarizes the most recent advances of Pichia pastoris as an efficient platform for recombinant protein production in the past 5 years, including innovative genetic tools and methodologies for manipulating gene expression systems, novel pathways in metabolic engineering, and the application of high-throughput screening coupled with mathematical models.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lukas Rieder, Katharina Ebner, Anton Glieder, Morten Sorlie
Summary: The study established a method to express fungal LPMOs using a Pichia pastoris platform, achieving high yields and easy purification. It is the first report that a yeast-derived signal peptide can successfully process LPMOs, forming fully functional enzymes.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biochemistry & Molecular Biology
Austin B. Schwartz, Anshika Kapur, Zhenbo Huang, Raveendra Anangi, John M. Spear, Scott Stagg, Erminia Fardone, Zolan Dekan, Jens T. Rosenberg, Samuel C. Grant, Glenn F. King, Hedi Mattoussi, Debra Ann Fadool
Summary: The olfactory system is modulated by metabolic factors to drive changes in feeding behavior and energy homeostasis. Inhibiting Kv1.3 in the olfactory bulb can enhance excitability of output neurons and lead to metabolic responses, resulting in changes in body weight and energy metabolism.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Fisheries
Josephine Nocillado, Peter Palma, Tianfang Wang, Evelyn Grace de Jesus-Ayson, Berta Levavi-Sivan, Abigail Elizur
Summary: This study demonstrates the potential of using yeast as a vehicle to orally deliver recombinant gonadotropin-releasing hormone (rGnRH1) for stimulating gonadal development in juvenile fish. The results show that the recombinant GnRH1 expressed in yeast exhibits both in vitro and in vivo biological activity. This technology has the potential to provide a non-invasive method for managing the reproduction of large, late-maturing fish species and delicate endangered species.
Article
Biochemistry & Molecular Biology
Kristina R. Denisova, Nikita A. Orlov, Sergey A. Yakimov, Elena A. Kryukova, Dmitry A. Dolgikh, Mikhail P. Kirpichnikov, Alexey V. Feofanov, Oksana V. Nekrasova
Summary: Peptide pore blockers and their fluorescent derivatives are important molecular probes for studying the Kv1.3 channel. GFP-MgTx, a fluorescent ligand based on green fluorescent protein (GFP) and margatoxin (MgTx), can recognize and evaluate Kv1.3 pore blockers of peptide origin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Carlos Eduardo de Oliveira Nascimento, Lorena Caixeta de Oliveira Simoes, Josiani de Cassia Pereira, Ronivaldo Rodrigues da Silva, Evandro Antonio de Lima, Gabriel Cimonetti de Almeida, Ana Lucia Barretto Penna, Mauricio Boscolo, Eleni Gomes, Roberto da Silva
Summary: This study produced Xylooligosaccharides (XOs) by extracting xylan from sugarcane bagasse and hydrolyzing it using an enzyme. The XOs stimulated the growth of beneficial intestinal bacteria and could be used as a supplement to promote the growth of probiotic strains.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shuochang Chen, Qiuping Tong, Xiaolei Guo, Hao Cong, Zi Zhao, Wenfeng Liang, Jiemin Li, Ping Zhu, Hui Yang
Summary: This study compared three signal peptides from alpha-mating factor (alpha-MF), inulinase (INU), and native levansucrase (LS) for their secretion efficiency in Pichia pastoris GS115. The results showed that the alpha-MF signal peptide achieved the first complete secretion of bacterial levansucrase in yeasts under methanol induction. The secreted recombinant levansucrase was glycosylated, which not only improved the enzyme's thermostability but also reversed the products profiles to favor the synthesis of high molecular weight (HMW) levan. This study provides a promising enzymatic approach for effectively producing HMW levan from sucrose resources.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Multidisciplinary Sciences
Sandy S. Pineda, Yanni K. -Y. Chin, Eivind A. B. Undheim, Sebastian Senff, Mehdi Mobli, Claire Dauly, Pierre Escoubas, Graham M. Nicholson, Quentin Kaas, Shaodong Guo, Volker Herzig, John S. Mattick, Glenn F. King
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Radiology, Nuclear Medicine & Medical Imaging
Junior Gonzales, Giacomo Pirovano, Chun Yuen Chow, Paula Demetrio de Souza Franca, Lukas M. Carter, Julie K. Klint, Navjot Guru, Jason S. Lewis, Glenn F. King, Thomas Reiner
Review
Pharmacology & Pharmacy
Volker Herzig, Ben Cristofori-Armstrong, Mathilde R. Israel, Samantha A. Nixon, Irina Vetter, Glenn F. King
BIOCHEMICAL PHARMACOLOGY
(2020)
Article
Pharmacology & Pharmacy
Darshani B. Rupasinghe, Volker Herzig, Irina Vetter, Zoltan Dekan, John Gilchrist, Frank Bosmans, Paul F. Alewood, Richard J. Lewis, Glenn F. King
BIOCHEMICAL PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Samantha A. Nixon, Zoltan Dekan, Samuel D. Robinson, Shaodong Guo, Irina Vetter, Andrew C. Kotze, Paul F. Alewood, Glenn F. King, Volker Herzig
Article
Biochemistry & Molecular Biology
Austin B. Schwartz, Anshika Kapur, Zhenbo Huang, Raveendra Anangi, John M. Spear, Scott Stagg, Erminia Fardone, Zolan Dekan, Jens T. Rosenberg, Samuel C. Grant, Glenn F. King, Hedi Mattoussi, Debra Ann Fadool
Summary: The olfactory system is modulated by metabolic factors to drive changes in feeding behavior and energy homeostasis. Inhibiting Kv1.3 in the olfactory bulb can enhance excitability of output neurons and lead to metabolic responses, resulting in changes in body weight and energy metabolism.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Anesthesiology
Fernanda C. Cardoso, Joel Castro, Luke Grundy, Gudrun Schober, Sonia Garcia-Caraballo, Tianjiao Zhao, Volker Herzig, Glenn F. King, Stuart M. Brierley, Richard J. Lewis
Summary: The discovery of two novel tarantula-venom peptides, Tap1a and Tap2a, provides potential treatment for chronic visceral pain by modulating the activity of Na-v and Ca(v)3 channels. Tap1a, in particular, shows significant analgesic effects in a mouse model, suggesting a new therapeutic approach for chronic pain.
Review
Pharmacology & Pharmacy
David A. Eagles, Chun Yuen Chow, Glenn F. King
Summary: This review examines the role of Na(V)1.7 channels in nociception, their history as a therapeutic target, and the challenges in developing potent inhibitors. Despite significant progress in drug development, effective analgesia remains elusive in clinical trials.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Junior Gonzales, Javier Hernandez-Gil, Thomas C. Wilson, Dauren Adilbay, Mike Cornejo, Paula Demetrio de Souza Franca, Navjot Guru, Christina Schroeder, Glenn F. King, Jason S. Lewis, Thomas Reiner
Summary: Due to the potential severity and persistence of chemotherapy-induced peripheral neuropathy, there is an urgent need for a tool to quantitatively measure this nerve damage. This study demonstrates a novel approach to localize peripheral nerves using in-cell heat imaging and fluorescence imaging, laying the groundwork for future clinical diagnostics and treatments.
MOLECULAR PHARMACEUTICS
(2021)
Article
Food Science & Technology
Laura C. Wait, Andrew A. Walker, Glenn F. King
Summary: Assassin bugs possess venom that contains proteins with a solitary CUB domain and cystatins, which may contribute to the insecticidal activity of their venom. These components have unknown functions in assassin bug venoms but have been found to cause paralysis and death in certain prey species upon injection.
Article
Biotechnology & Applied Microbiology
Markus Muttenthaler, Glenn E. King, David J. Adams, Paul E. Alewood
Summary: This Perspective summarizes key trends in peptide drug discovery and development, including human hormones, elegant medicinal chemistry and rational design strategies, peptide drugs derived from nature, and major breakthroughs in molecular biology and peptide chemistry. It also emphasizes lessons from earlier approaches that are still relevant today and discusses emerging strategies impact on peptide drug discovery.
NATURE REVIEWS DRUG DISCOVERY
(2021)
Article
Food Science & Technology
Lauren M. Ashwood, Michela L. Mitchell, Bruno Madio, David A. Hurwood, Glenn F. King, Eivind A. B. Undheim, Raymond S. Norton, Peter J. Prentis
Summary: This study found that morphological variations in tentacles are associated with distinct toxin gene expression levels. Sea anemones with spherical tentacular structures may use specific venom cocktails to protect branched structures hosting a large number of photosynthetic symbionts.
Article
Biochemistry & Molecular Biology
Volker Herzig, Yong-Cyuan Chen, Yanni K-Y Chin, Zoltan Dekan, Yu-Wang Chang, Hui-Ming Yu, Paul F. Alewood, Chien-Chang Chen, Glenn F. King
Summary: This study identified a tarantula venom from Avicularia spec. that can inhibit specific subtypes of T-type calcium channels, with the active toxin being omega-Avsp1a. The research suggests that omega-Avsp1a can inhibit specific subtypes of T-type calcium channels, and the domain-1 of Ca(V)3.3 is crucial for this inhibitory effect.
Article
Biochemistry & Molecular Biology
Fernanda C. Cardoso, Sandy S. Pineda, Volker Herzig, Kartik Sunagar, Naeem Yusuf Shaikh, Ai-Hua Jin, Glenn F. King, Paul F. Alewood, Richard J. Lewis, Sebastien Dutertre
Summary: Australian tree-dwelling funnel-web spiders have venom that induces deadly symptoms and exhibits different venom composition compared to ground-dwelling species. The venom of tree-dwellers strongly modulates human voltage-gated sodium and calcium channels, while also showing potent insecticidal effects. This study contributes to understanding the molecular and pharmacological basis for severe envenomation by Australian tree-dwelling funnel-web spiders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Andrew A. Walker, Lynda E. Perkins, Andrea Battisti, Myron P. Zalucki, Glenn F. King
Summary: Ochrogaster lunifer is an Australian caterpillar that has detachable urticating setae with defensive function. These setae can cause inflammation on human skin and equine foetal loss syndrome in gravid horses if accidentally ingested. Transcriptomics and proteomics analysis identified 37 putative toxins, including multiple homologues of honeybee venom peptide secapin and proteins with similarity to odorant binding proteins, arylphorins, and the insect immune modulator Diedel. This study reveals candidate molecules that may contribute to the adverse effects of processionary caterpillar setae on human and animal health.