Article
Reproductive Biology
Santhanahalli Siddalingappa Archana, Sellappan Selvaraju, Arunachalam Arangasamy, Balakrishnan Binsila, Divakar Swathi, Laxman Ramya, Arumugam Kumaresan, Balaganur Krishnappa
Summary: This study assessed the impact of spermatozoa surface antigenic proteins on the functional competence of bovine neat and frozen-thawed semen. It was found that samples with lower antigenicity exhibited better performance in certain kinematic parameters and sperm function. Additionally, higher seminal antigenicity was associated with reduced mitochondrial membrane potential and acrosome reaction ability in post-thaw spermatozoa.
Article
Andrology
Gabriela Hernandez-Silva, Aidee S. Lopez-Torres, Israel Maldonado-Rosas, Esperanza Mata-Martinez, Fernando Larrea, Victor Torres-Flores, Claudia L. Trevino, Mayel Chirinos
Summary: The study compared the functional differences between human sperm samples processed by swim-up and density gradient centrifugation, revealing significant variations in redox state, intracellular calcium oscillations, hyperactivation, protein tyrosine phosphorylation, and acrosome reaction. Sperm retrieved by density gradient centrifugation exhibited higher levels of hyperactivation and tyrosine phosphorylation compared to swim-up sperm, suggesting a greater degree of capacitation.
WORLD JOURNAL OF MENS HEALTH
(2021)
Article
Veterinary Sciences
Giacomo Galdiero, Emanuele D'Anza, Cristina de Angelis, Sara Albarella, Vincenzo Peretti, Rosario Pivonello, Francesca Ciotola
Summary: Male infertility is a growing issue in both humans and animals, and understanding its causes is crucial for identifying new diagnostic and therapeutic strategies. This study discovered a correlation between sperm global DNA methylation (SGDM) levels and conventional seminal parameters in dogs, suggesting SGDM could serve as a marker of testis function and spermatogenesis.
VETERINARY SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Chiara Castellini, Mario Muselli, Antonio Parisi, Maria Totaro, Daniele Tienforti, Giuliana Cordeschi, Macro Giorgio Baroni, Mauro Maccarrone, Stefano Necozione, Sandro Francavilla, Arcangelo Barbonetti
Summary: This meta-analysis study found a weak negative association between urinary levels of BPA and sperm motility. The overall estimates of data produced by clinical studies indicate a clinically negligible association between urinary BPA concentrations and semen quality.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Sameh A. Abdelnour, Mahmoud A. E. Hassan, Ahmed. M. Shehabeldin, Mohamed. E. A. Omar, Wael A. Khalil, Reham Mokhtar Aman
Summary: The effect of propolis-loaded in nanoliposomes (PRNL) on the quality of cryopreserved buffalo semen was investigated. The addition of 2 or 4μg/mL of PRNL significantly improved sperm motility, viability, and membrane integrity, while reducing abnormalities and chromatin damage.
SCIENTIFIC REPORTS
(2023)
Article
Reproductive Biology
Olatunji Abubakar Jimoh, Wahab Adekunle Oyeyemi, Hafsat Ololade Okin-Aminu, Bolaji Fatai Oyeyemi
Summary: This study found that mistletoe supplementation in bucks' diets significantly increased sperm concentration, progressive motility, velocity, and head amplitude compared to the control group. The inclusion of herbal supplements linearly increased seminal total antioxidant activity while decreasing seminal lipid peroxidation levels. Bucks fed on herbal supplements showed depleted gonadal and extra-gonadal sperm reserves compared to the control group.
Article
Agriculture, Dairy & Animal Science
Chiara Del Prete, Alfonso Calabria, Valentina Longobardi, Veronica Palumbo, Barbara Merlo, Eleonora Iacono, Simona Tafuri, Domenico Carotenuto, Francesca Ciani, Sara Damiano, Roberto Ciarcia, Natascia Cocchia
Summary: The addition of Maca extract to canine chilled extender showed positive effects on sperm hyperactivation and DNA integrity in short-term storage. However, high concentrations of Maca had immediately deleterious effects on semen quality. Maca supplementation also increased sperm hyperactivation, preserved DNA integrity, and reduced lipid peroxidation in short-term storage.
Article
Endocrinology & Metabolism
Giulia Traini, Lara Tamburrino, Linda Vignozzi, Elisabetta Baldi, Sara Marchiani
Summary: This study evaluated the association between oxidized viable spermatozoa and sperm functions using two probes. The results showed a positive correlation between oxidized viable spermatozoa and sperm basal parameters, and a negative correlation with sperm DNA fragmentation. In addition, viable oxidized spermatozoa did not exhibit apoptotic features.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Genetics & Heredity
Chuan Huang, Qian Liu, Zhong-Wei Wang, Wen-Jun Zhou, Zeng-Hui Huang, Xi-Ren Ji, Xue-Feng Luo, Yu-Ling Tang, Li-Qing Fan, Wen-Bing Zhu
Summary: The study found that abstinence time was significantly associated with semen parameters and donor eligibility. Increasing abstinence time before donation may be meaningful in improving the proportion of eligible sperm donors.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Agriculture, Dairy & Animal Science
Eneiva Carla Carvalho Celeghini, Maira Bianchi Rodrigues Alves, Rubens Paes de Arruda, Gabriela Marques de Rezende, Shirley Andrea Florez-Rodriguez, Manoel Francisco de Sa Filho
Summary: This study investigated the efficiency of CellROX Deep Red (R) and Orange (R) probes in detecting ROS in bovine sperm cells, finding that higher sperm ROS levels detected by CellROX Red (R) might be associated with low fertility samples. The coefficients of determination for Red and Orange probes were 0.61 and 0.56, respectively, indicating their effectiveness in detecting ROS. Overall, both CellROX Red (R) and Orange (R) were shown to be efficient in detecting ROS in bovine spermatozoa.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Monike Quirino, Ulrike Jakop, Ana Paula Goncalves Mellagi, Fernando Pandolfo Bortolozzo, Markus Jung, Martin Schulze
Summary: This study evaluated the effect of different proportions of dead spermatozoa on the quality of liquid boar semen. The results showed that dead sperm did not have a substantial impact on the quality of live sperm.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Agriculture, Dairy & Animal Science
Kristyna Petricakova, Martina Janosikova, Martin Ptacek, Lukas Zita, Filipp Georgijevic Savvulidi, Agnieszka Partyka
Summary: Sperm cryopreservation is an important strategy for threatened livestock populations. This study compared different commercial extenders for the cryopreservation of Czech Golden Spotted Hen semen and proposed an important tool. The results showed that modified Poultry media (R) and Raptac (R) extenders were suitable for sperm cryopreservation.
Article
Agriculture, Dairy & Animal Science
Wael A. Khalil, Mahmoud A. E. Hassan, Mostafa A. El-Harairy, Sameh A. Abdelnour
Summary: In this study, the effects of Thymoquinone nanoparticles (TQNPs) on frozen-thawed buffalo sperm were investigated. TQNPs supplementation improved sperm quality, including membrane integrity and progressive motility. TQNPs also reduced oxidative stress and improved antioxidant capacity. Additionally, TQNPs decreased acrosome exocytosis and apoptosis-like changes. The use of TQNPs-treated sperm resulted in higher non-return rates in buffalo cows, indicating improved fertility.
Article
Biochemistry & Molecular Biology
Xiao Shi, Carol Pui Shan Chan, Grace Ka Yan Man, David Yiu Leung Chan, Ming Hung Wong, Tin-Chiu Li
Summary: The study found that blood Pb concentration was negatively associated with sperm morphology, blood Cd concentration was negatively associated with sperm acrosome reaction, blood Mo concentration was positively associated with semen volume but negatively associated with sperm vitality at high levels, blood Zn concentration was positively associated with sperm vitality, blood Fe concentration was positively associated with sperm acrosome reaction, and blood Ca concentration was positively or negatively associated with sperm morphology and semen volume respectively.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Susmita Ghosh, Swapneil Parikh, Mehar Un Nissa, Arup Acharjee, Avinash Singh, Dhruv Patwa, Prashant Makwana, Arundhati Athalye, Abhilash Barpanda, Malini Laloraya, Sanjeeva Srivastava, Firuza Parikh
Summary: COVID-19 has negative effects on male fertility, altering the semen proteome and impairing reproductive function.
Review
Infectious Diseases
L. de Thurah, J. Bonde, J. U. H. Lam, M. Rebolj
CLINICAL MICROBIOLOGY AND INFECTION
(2018)
Article
Virology
Jesper Bonde, Ditte Moller Ejegod, Kate Cuschieri, Joakim Dillner, Danielle A. M. Heideman, Wim Quint, Miguel Angel Pavon Ribas, Elizaveta Padalko, Irene Kraus Christiansen, Lan Xu, Marc Arbyn
JOURNAL OF CLINICAL VIROLOGY
(2018)
Article
Oncology
Jesper Bonde, Fabio Bottari, Valentin Parvu, Helle Pedersen, Karen Yanson, Anna D. Iacobone, Salma Kodsi, Fabio Landoni, Laurence Vaughan, Ditte M. Ejegod, Maria T. Sandri
INTERNATIONAL JOURNAL OF CANCER
(2019)
Article
Oncology
Frederique J. Vink, Chris J. L. M. Meijer, Gary M. Clifford, Mario Poljak, Anja Ostrbenk, Karl Ulrich Petry, Beate Rothe, Jesper Bonde, Helle Pedersen, Silvia de Sanjose, Montserrat Torres, Marta del Pino, Wim G. Quint, Kate Cuschieri, Elia Alcaniz Boada, Nienke E. van Trommel, Birgit Lissenberg-Witte, Arno N. Floore, Albertus T. Hesselink, Renske D. M. Steenbergen, Maaike C. G. Bleeker, Danielle A. M. Heideman
INTERNATIONAL JOURNAL OF CANCER
(2020)
Article
Virology
D. A. M. Heideman, L. Xu, A. T. Hesselink, S. Doorn, D. M. Ejegod, H. Pedersen, W. G. Quint, J. Bonde, M. Arbyn
JOURNAL OF CLINICAL VIROLOGY
(2019)
Article
Virology
Ditte Moller Ejegod, Mona Hansen, Irene Kraus Christiansen, Helle Pedersen, Wim Quint, Lan Xu, Marc Arbyn, Jesper Bonde
JOURNAL OF CLINICAL VIROLOGY
(2020)
Article
Oncology
Jesper Bonde, Arno Floore, Ditte Ejegod, Frederique J. Vink, Albertus Hesselink, Peter M. van de Ven, Anja Ostrbenk Valencak, Helle Pedersen, Saskia Doorn, Wim G. Quint, Karl Ulrich Petry, Mario Poljak, Grazyna Stanczuk, Kate Cuschieri, Silvia de Sanjose, Maaike Bleeker, Johannes Berkhof, Chris J. L. M. Meijer, Danielle A. M. Heideman
Summary: The FAM19A4/miR124-2 methylation test shows a high sensitivity of 95% and specificity of 78.3% as a molecular triage test for HPV-positive cervical cancer screening. It can be considered as an effective alternative/supplement to cytology for cervical cancer screening.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Oncology
Frederique J. Vink, Stefanie Dick, Danielle A. M. Heideman, Lise M. A. De Strooper, Renske D. M. Steenbergen, Birgit I. Lissenberg-Witte, Arno Floore, Jesper H. Bonde, Anja Ostrbenk Valencak, Mario Poljak, Karl U. Petry, Peter Hillemanns, Nienke E. van Trommel, Johannes Berkhof, Maaike C. G. Bleeker, Chris J. L. M. Meijer
Summary: This study evaluates the immunohistochemical expression of p16(ink4a) and Ki-67, known as the immunoscore (IS), in relation to HPV E4 expression and FAM19A4/miR124-2 methylation in high-grade CIN lesions. The findings suggest that these biomarker profiles may help clinicians in decision-making regarding immediate treatment or observation to avoid unnecessary overtreatment of high-grade CIN lesions.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Immunology
Frederique J. Vink, Chris J. L. M. Meijer, Albertus T. Hesselink, Arno N. Floore, Birgit Lissenberg-Witte, Jesper H. Bonde, Helle Pedersen, Kate Cuschieri, Ramya Bhatia, Mario Poljak, Anja Ostrbenk Valencak, Peter Hillemanns, Wim G. Quint, Marta del Pino, Gemma G. Kenter, Renske D. M. Steenbergen, Danielle A. M. Heideman, Maaike C. G. Bleeker
Summary: The FAM19A4/miR124-2 methylation test can detect nonproductive, transforming CIN2/3 lesions with high specificity in HPV-positive women under 30 years old, providing valuable information for treatment decisions.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Public, Environmental & Occupational Health
Ditte Moller Ejegod, Helle Pedersen, Birgitte Tonnes Pedersen, Reza Serizawa, Jesper Bonde
Summary: The Danish cervical cancer screening program is a free cancer prevention program targeting women aged 23-64. Despite a declining attendance rate, nearly half of all new cervical cancer cases are found in non-attenders. To increase attendance, the Capital Region of Denmark introduced HPV self-sampling as an alternative option, resulting in a 27% participation rate. Overall, 31% of the invited women were screened.
PREVENTIVE MEDICINE
(2022)
Letter
Infectious Diseases
Marc Arbyn, Kate Cuschieri, Mario Poljak, Jesper Bonde
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Public, Environmental & Occupational Health
Marc Arbyn, Sharonjit Kaur Dhillon, Marianna Martinelli, Cindy Simoens, Lize Cuypers, Jannes Bode, Marc Van Ranst, Philippe Corbisier, Jesper Bonde, Clementina Cocuzza
Summary: VALCOR is a study protocol aimed at establishing a framework for the validation of SARS-CoV-2 virus assays. Its objectives are to define common criteria for SARS-CoV-2 assays and generate comprehensive validation data.
ARCHIVES OF PUBLIC HEALTH
(2022)
Article
Ophthalmology
Ingvild Ramberg, Nicolai Christian Sjo, Jesper Hansen Bonde, Steffen Heegaard
BMJ OPEN OPHTHALMOLOGY
(2019)
Article
Virology
Ditte Moller Ejegod, Helle Pedersen, Garazi Pena Alzua, Camilla Pedersen, Jesper Bonde
PAPILLOMAVIRUS RESEARCH
(2018)
Article
Oncology
J. U. H. Lam, K. M. Elfstrom, D. M. Ejegod, H. Pedersen, C. Rygaard, M. Rebolj, E. Lynge, K. E. Juul, S. K. Kjaer, J. Dillner, J. Bonde
BRITISH JOURNAL OF CANCER
(2018)