Article
Oncology
Serina Cheung, Pallavi Jain, Jonathan So, Saeid Shahidi, Stephen Chung, Marianne Koritzinsky
Summary: Targeting the p38 MAPK protein kinase can inhibit growth and survival of castration-resistant prostate cancer cells, regardless of oxygen levels, and may prolong the survival of tumor-bearing mice. This demonstrates the potential of p38 MAPK inhibition as a therapeutic strategy for disrupting AR signaling in the heterogeneous CRPC tumor microenvironment.
Article
Biology
Anna L. Hoeving, Julian Schmitz, Kazuko E. Schmidt, Johannes F. W. Greiner, Cornelius Knabbe, Barbara Kaltschmidt, Alexander Gruenberger, Christian Kaltschmidt
Summary: This study showed the positive impact of human blood serum on the migration behavior of cardiac stem cells, dependent on p38-MAPK kinase. The findings emphasize the potential of microfluidic cultivation devices for assessing spatio-temporal migration dynamics of adult human stem cells on a single-cell level.
Article
Anatomy & Morphology
Hongfang Meng, Fen Wei, Zhiqiang Ge, Jide Jin, Hua Wang, Li-sheng Wang, Chu-tse Wu
Summary: This study verified the effects of long-term hypoxic culture on human dental pulp stem cells (hDPSCs). The results showed that hypoxic culture maintained the morphology, phenotype, and proliferation activity of hDPSCs, while reducing cell senescence. Hypoxic culture also increased the expression of stemness markers and decreased the expression of senescence-related genes. Additionally, hypoxic culture suppressed spontaneous and induced osteogenic and adipogenic differentiation. These findings highlight the beneficial effects of long-term hypoxic culture on hDPSCs' biological characteristics.
Article
Geriatrics & Gerontology
Tianjiao Tang, Lingling Xie, Song Hu, Lingling Tan, Xiaozhen Lei, Xiaozhen Luo, Ling Yang, Ming Yang
Summary: CCR and SI have satisfactory and comparable diagnostic accuracy and prognostic values for sarcopenia in patients with advanced NSCLC, and can serve as surrogate biomarkers for evaluating sarcopenia in these patients.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Neurosciences
Lingling Jiao, Tongying Xu, Xixun Du, Xi Chen, Qian Jiao, Hong Jiang
Summary: This study examined the physiological effects of sodium nitroprusside (SNP) in the early developmental stage of the nervous system using differentiated neural stem cells (NSCs). The results showed that SNP at certain concentrations reduced cell viability and increased cleaved caspase 3 levels, but had no significant effects on neuronal differentiation. The levels of phosphorylated p38 were upregulated with increasing concentrations of SNP and p38 was found to be involved in the cell survival of NSCs regulated by NO.
Article
Surgery
Gokay Baykara, Nezih Sungur, Kadri Ozer, Oguz Atan, Muzaffer Caydere, Pinar Nercis Kosar, Ugur Kocer
Summary: The study investigated the effects of autologous conditioned serum and platelet-rich plasma on fat graft outcomes. The results showed that autologous conditioned serum and platelet-rich plasma improved fat graft survival compared to saline, and autologous conditioned serum was associated with less inflammation, greater fat viability, and more integrity.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2022)
Article
Biochemistry & Molecular Biology
Kanwal Haneef, Asmat Salim, Zehra Hashim, Amber Ilyas, Basir Syed, Aftab Ahmed, Shamshad Zarina
Summary: This study investigated the mechanism by which hypoxic preconditioning enhances the survival of mesenchymal stem cells (MSCs). Proteomic analysis revealed higher expression of certain proteins in hypoxic preconditioned (HP) MSCs, which are possibly involved in cellular survival and proliferation through various cellular pathways. This research is important for understanding the processes involved in hypoxic preconditioning of MSCs and designing cell-based therapeutic strategies for tissue regeneration.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Otorhinolaryngology
Gorkem Eskiizmir, Gizem Calibasi Kocal, Tugba Uysal, Hulya Ellidokuz, Yasemin Baspinar
Summary: This study investigated the diagnostic, predictive, and prognostic roles of hypoxia-related biomolecules in laryngeal neoplasms. The study found that hypoxia-inducible factor-2 is a prognostic factor in larynx cancer irrespective of treatment modality.
CLINICAL OTOLARYNGOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Parisa Fayyazpour, Effat Alizadeh, Vahid Hosseini, Ashkan Kalantary-Charvadeh, Mitra Niafar, Vahideh Sadra, Zahra Norouzi, Aysan Saebnazar, Amir Mehdizadeh, Masoud Darabi
Summary: In type 2 diabetes, dyslipidemia and increased serum free fatty acids (FFAs) negatively affect insulin secretion and worsen the disease. This study found that FFAs from diabetic serum have a detrimental effect on the stemness characteristics of adipose-derived mesenchymal stem cells, potentially impairing their regenerative capabilities.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Dentistry, Oral Surgery & Medicine
T. Zou, S. Jiang, Y. Zhang, J. Liu, B. Yi, Y. Qi, W. L. Dissanayaka, C. Zhang
Summary: The study fabricated oxygen-generating scaffolds using GelMA material to enhance cell survival. Results showed that 0.5% CaO2-GelMA hydrogels were effective in maintaining SCAP viability and improving cell survival within root canals.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ramada R. Khasawneh, Ejlal Abu -El -Rub
Summary: Mesenchymal stem cells (MSCs) have shown great therapeutic potential in treating diseases, but face challenges related to oxygen levels. Moderate hypoxia enhances MSCs' functions, while severe hypoxia negatively affects their therapeutic potential and survival. Research findings indicate that severe hypoxia impairs the cell-cell adhesion abilities of MSCs, potentially affecting their incorporation at the transplantation site.
CELLULAR AND MOLECULAR BIOLOGY
(2022)
Article
Genetics & Heredity
Stuart J. Grice, Ji-Long Liu
Summary: Spinal muscular atrophy is a neurodegenerative disease characterized by motor neuron loss and impaired motor function. This study found that neurodevelopmental defects precede locomotor dysfunction and that motor neuron sensitivity to SMN loss may arise before synapse formation and maturation.
Article
Medicine, Research & Experimental
Miranda K. Culley, Jingsi Zhao, Yi Yin Tai, Ying Tang, Dror Perk, Vinny Negi, Qiujun Yu, Chen-Shan C. Woodcock, Adam Handen, Gil Speyer, Seungchan Kim, Yen-Chun Lai, Taijyu Satoh, Annie M. M. Watson, Yassmin Al Aaraj, John Sembrat, Mauricio Rojas, Dmitry Goncharov, Elena A. Goncharova, Omar F. Khan, Daniel G. Anderson, James E. Dahlman, Aditi U. Gurkar, Robert Lafyatis, Ahmed U. Fayyaz, Margaret M. Redfield, Mark T. Gladwin, Marlene Rabinovitch, Mingxia Gu, Thomas Bertero, Stephen Y. Chan
Summary: The study reveals that endothelial cell senescence in pulmonary hypertension (PH) is associated with Fe-S deficiency, and Frataxin (FXN) protein may control endothelial cell senescence. FXN deficiency leads to senescence of vascular endothelial cells, promoting vascular inflammation and proliferative signals, driving disease progression.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Cell Biology
Ain Zubaidah Ayob, Thamil Selvee Ramasamy
Summary: Tumour hypoxia and nutrient deprivation stress can promote the development of cancer cell stem-like characteristics, while exacerbating tumour aggressiveness and resistance. HepG2 tumoursphere culture under prolonged hypoxic and serum-free conditions revealed significant changes in morphology and proliferative capacity.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2021)
Article
Biochemistry & Molecular Biology
Mark-Alexander Schwarzbich, Hao Dai, Lambros Kordelas, Dietrich W. Beelen, Aleksandar Radujkovic, Carsten Mueller-Tidow, Peter Dreger, Thomas Luft
Summary: The study demonstrates that pre-conditioning serum Leptin levels can serve as an independent prognostic marker for relapse following alloSCT in high-risk acute leukemia patients, particularly those in the intermediate to advanced stages. This effect was validated in an independent cohort, suggesting the potential utility of serum Leptin for predicting post-transplant outcomes in certain patient populations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Chia-Sheng Chu, Chi-Ying Yang, Chun-Chieh Yeh, Ro-Ting Lin, Chi-Ching Chen, Li-Yuan Bai, Mien-Chie Hung, Chun-Che Lin, Chun-Ying Wu, Jaw-Town Lin
Summary: Investigating the intra-tumoral microbiome through endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) can provide adequate fresh pancreatic cancer tissue for research. This study found that the microbiome profiles obtained from EUS-FNB and surgical biopsy were comparable in terms of alpha-diversity and taxonomic composition.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Yunfei Wen, Anca Chelariu-Raicu, Sujanitha Umamaheswaran, Alpa M. Nick, Elaine Stur, Pahul Hanjra, Dahai Jiang, Nicholas B. Jennings, Xiuhui Chen, Sara Corvigno, Deanna Glassman, Gabriel Lopez-Berestein, Jinsong Liu, Mien-Chie Hung, Anil K. Sood
Summary: Anti-angiogenic therapies, such as anti-VEGF antibodies, have shown promise clinically but often face adaptive resistance. Research on adaptive resistance to AVA therapy in ovarian cancer models reveals mechanisms involving endothelial p130cas, VEGFR2 cleavage, and autophagy. Targeting these pathways, such as blocking autophagy or genomic ablation of vascular p130cas, can significantly improve survival in AVA-resistant ovarian tumors, providing new strategies for overcoming adaptive resistance.
Article
Gastroenterology & Hepatology
Chenhao Zhou, Jialei Weng, Yuan Gao, Chunxiao Liu, Xiaoqiang Zhu, Qiang Zhou, Chia-Wei Li, Jialei Sun, Manar Atyah, Yong Yi, Qinghai Ye, Yi Shi, Qiongzhu Dong, Yingbin Liu, Mien-Chie Hung, Ning Ren
Summary: By analyzing immune-related genes, we identified a prognostic signature that is associated with the prognosis and immunotherapy response in hepatocellular carcinoma (HCC) patients. This signature accurately predicts overall survival rates and can assist in personalized clinical management.
JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY
(2022)
Article
Oncology
Xixi Zhao, Yongkun Wei, Yu-Yi Chu, Yintao Li, Jung-Mao Hsu, Zhou Jiang, Chunxiao Liu, Jennifer L. Hsu, Wei-Chao Chang, Riyao Yang, Li-Chuan Chan, Jingkun Qu, Shuqun Zhang, Haoqiang Ying, Dihua Yu, Mien-Chie Hung
Summary: This study reveals the role of CK2 in immunosuppression by phosphorylation and stabilization of PD-L1, and suggests CK2 inhibition as a potential immunotherapeutic approach for treating cancer.
Article
Biochemistry & Molecular Biology
Jong-Ho Cha, Li-Chuan Chan, Ying-Nai Wang, Yu-Yi Chu, Chie-Hong Wang, Heng-Huan Lee, Weiya Xia, Woei-Cherng Shyu, Shih-Ping Liu, Jun Yao, Chiung-Wen Chang, Fan-Ru Cheng, Jielin Liu, Seung-Oe Lim, Jennifer L. Hsu, Wen-Hao Yang, Gabriel N. Hortobagyi, Chunru Lin, Liuqing Yang, Dihua Yu, Long-Bin Jeng, Mien-Chie Hung
Summary: The expression of EphA10, a receptor tyrosine kinase, is associated with tumor progression and poor prognosis in several malignancies. This study demonstrates high expression levels of EphA10 in tumor regions and immune suppressive myeloid cells in the tumor microenvironment. The development of anti-EphA10 monoclonal antibodies and chimeric antigen receptor-T cell therapy targeting EphA10 shows potential as a promising strategy for patients with EphA10-positive tumors.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Riyao Yang, Linlin Sun, Ching-Fei Li, Yu-Han Wang, Weiya Xia, Boning Liu, Yu-Yi Chu, Laura Bover, Long Vien, Mien-Chie Hung
Summary: This study reports the development of two novel Gal-9-neutralizing antibodies that effectively protect human T cells from Gal-9-induced cell death and promote T cell-mediated killing of tumor cells. These findings demonstrate the potential of targeting Gal-9 for cancer immunotherapy.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Jian Tu, Zijun Huo, Yao Yu, Dandan Zhu, An Xu, Mo-Fan Huang, Ruifeng Hu, Ruoyu Wang, Julian A. Gingold, Yi-Hung Chen, Kuang-Lei Tsai, Nicolas R. Forcioli-Conti, Sarah X. L. Huang, Thomas R. Webb, Jie Su, Danielle A. Bazer, Peilin Jia, Jason T. Yustein, Lisa L. Wang, Mien-Chie Hung, Zhongming Zhao, Chad D. Huff, Jingnan Shen, Ruiying Zhao, Dung-Fang Lee
Summary: The study reveals that the spliceosome is an up-regulated target in RB1-mutant cells responding to oncogenic stress, and pRB and E2F3a coregulate spliceosomal gene expression, affecting cell proliferation and tumorigenesis. These findings suggest that the spliceosomal machinery could be a potential therapeutic vulnerability for pRB-deficient cancers.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Yun-Long Wang, Wan-Wen Zhao, Shao-Mei Bai, Li-Li Feng, Shu-Ying Bie, Li Gong, Fang Wang, Ming-Biao Wei, Wei-Xing Feng, Xiao-Lin Pang, Cao-Litao Qin, Xin-Ke Yin, Ying-Nai Wang, Weihua Zhou, Daniel R. Wahl, Quentin Liu, Ming Chen, Mien-Chie Hung, Xiang-Bo Wan
Summary: MRNIP forms liquid-like condensates to promote DSB repair. It concentrates the MRN complex to the damaged DNA in the nucleus, accelerating DSB sensing and end resection.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Wei-Jan Wang, Yeh Chen, Wen-Chi Su, Yen-Yi Liu, Wan-Jou Shen, Wei-Chao Chang, Sheng-Teng Huang, Cheng-Wen Lin, Yu-Chuan Wang, Chia-Shin Yang, Mei-Hui Hou, Yu-Chi Chou, Yang-Chang Wu, Shao-Chun Wang, Mien-Chie Hung
Summary: In this study, we identified a naturally derived compound called peimine, extracted from Fritillaria, that can inhibit viral infection of SARS-CoV-2 variants of concern (VOCs) in lung cells. Peimine blocks viral entry by interfering with the interaction between the spike protein and ACE2. Molecular docking analysis showed that peimine has a strong binding affinity for the N501Y mutation in the spike protein. Furthermore, Fritillaria was found to significantly inhibit SARS-CoV-2 viral infection. These findings suggest that peimine and Fritillaria could be potential drugs and food sources for COVID-19 patients.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Oncology
Hsiao-Fan Chen, Po-Ren Hsueh, Yen-Yi Liu, Yeh Chen, Sui-Yuan Chang, Wei-Jan Wang, Chen-Shiou Wu, Ya-Min Tsai, Yu-Shu Liu, Wen-Chi Su, Yu-Chi Chou, Mien-Chie Hung
Summary: Disulfiram has shown potential as a therapy against COVID-19 by inhibiting viral replication and blocking viral entry into host cells. It has demonstrated activity against different variants of SARS-CoV-2 in molecular and cellular biology assays.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Oncology
Shih-Han Wang, Wen-Cheng Chou, Hsiang -Chi Huang, Te-An Lee, Tzu-Chun Hsiao, Ling -Hui Wang, Ke-Bin Huang, Chun-Tse Kuo, Chi -Hong Chao, Shing-Jyh Chang, Jung -Mao Hsu, Jialei Weng, Ning Ren, Fu -An Li, Yun-Ju Lai, Chenhao Zhou, Mien-Chie Hung, Chia -Wei Li
Summary: N-linked glycosylation of proteins is a post-translational modification that protects tumor antigens from immune attack. This study explores the role of N-glycosylation of SLAMF7 in breast cancer progression and highlights the potential strategy of using ADC to enhance immunotherapeutic agents through deglycosylation.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Oncology
Yi-Chuan Li, Hirohito Yamaguchi, Yen-Yi Liu, Kai-Cheng Hsu, Ting-Hsuan Sun, Pei-Chi Sun, Mien-Chie Hung
Summary: This article investigates the interaction between ribonuclease 1 (RNase1) and EphA4 and reveals their structure and role in cellular activation. The study suggests that electrostatic force plays a crucial role in the binding and activation of RNase1 and EphA4.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Oncology
Chun-Te Ho, Shih-Pei Lin, Ling-Ming Tseng, Mien-Chie Hung, Shih-Chieh Hung
Summary: This study found that a mutant form of Snail reduced the expression of Src in luminal A breast cancer cells and enhanced their migration and invasion abilities, while reducing their capacity to form tumor spheres. Additionally, human luminal A breast cancer samples showed a negative correlation between Vimentin and Src expression.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Chen-Shiou Wu, Hsiu-Mei Chiang, Yeh Chen, Chung-Yu Chen, Hsiao-Fan Chen, Wen-Chi Su, Wei-Jan Wang, Yu-Chi Chou, Wei-Chao Chang, Shao-Chun Wang, Mien-Chie Hung
Summary: This research explores natural plant extracts that have the potential to combat SARS-CoV-2 and provide alternative options for prevention and disinfection. The study identifies coffee leaf extract as an effective inhibitor of SARS-CoV-2 infection in various strains. Additionally, the extract proves to be more potent at preventing viral entry into cells when applied topically than the standard disinfectant ethanol. Compounds such as caffeine, chlorogenic acid, quinic acid, and mangiferin are found to be associated with the extract's antiviral activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Immunology
Poyee Lau, Guanxiong Zhang, Shuang Zhao, Long Liang, Hailun Zhang, Guowei Zhou, Mien-Chie Hung, Xiang Chen, Hong Liu
Summary: This study reveals the important role of SPHK1 in tumor immunity and shows that its inhibition can suppress tumor growth and promote antitumor immunity. Additionally, SPHK1 and MTA3 are identified as biological markers for predicting the efficacy of anti-PD-1 mAb therapy in melanoma patients.
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)