Article
Multidisciplinary Sciences
Chao-Pei Liu, Zhenyu Yu, Jun Xiong, Jie Hu, Aoqun Song, Dongbo Ding, Cong Yu, Na Yang, Mingzhu Wang, Juan Yu, Peini Hou, Kangning Zeng, Zhenyu Li, Zhuqiang Zhang, Xinzheng Zhang, Wei Li, Zhiguo Zhang, Bing Zhu, Guohong Li, Rui-Ming Xu
Summary: This study provides direct knowledge about the histone binding mode and nucleosome assembly process of CAF-1. It reveals that one CAF-1 complex binds to one histone H3-H4 heterodimer through the p60 subunit and the acidic domain of the p150 subunit. It also identifies the formation of a dimeric CAF-1-H3-H4 supercomplex and the role of CAF-1 in facilitating right-handed DNA wrapping of H3-H4 tetramers.
Article
Multidisciplinary Sciences
Xizi Chen, Yilun Qi, Zihan Wu, Xinxin Wang, Jiabei Li, Dan Zhao, Haifeng Hou, Yan Li, Zishuo Yu, Weida Liu, Mo Wang, Yulei Ren, Ze Li, Huirong Yang, Yanhui Xu
Summary: This study elucidates the assembly mechanism of human TFIID-based PIC, showing two tracks for modular reorganization and direct promoter deposition, converging at a holo PIC with around 50 subunits. TFIID stabilizes PIC organization and supports CAK-mediated phosphorylation of Pol II.
Article
Multidisciplinary Sciences
Ju Zhou, Anhui Wang, Yinlong Song, Nan Liu, Jia Wang, Yan Li, Xin Liang, Guohui Li, Huiying Chu, Hong-Wei Wang
Summary: In this study, the authors explore the enigma of GTP-triggered microtubule assembly. The proposed flexible model emphasizes longitudinal and lateral contacts, enhancing our understanding of microtubule nucleation and assembly.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yi Zhou, Panayiotis E. Stevis, Jing Cao, Kei Saotome, Jiaxi Wu, Arielle Glatman Zaretsky, Sokol Haxhinasto, George D. Yancopoulos, Andrew J. Murphy, Mark W. Sleeman, William C. Olson, Matthew C. Franklin
Summary: This article presents the cryo-electron microscopy structures of five signaling complexes of the IL-6 family cytokines, revealing their assembly and molecular interactions. The structures provide insights for therapeutic targeting of gp130-mediated signaling.
Article
Plant Sciences
Jure Zabret, Stefan Bohn, Sandra K. Schuller, Oliver Arnolds, Madeline Moller, Jakob Meier-Credo, Pasqual Liauw, Aaron Chan, Emad Tajkhorshid, Julian D. Langer, Raphael Stoll, Anja Krieger-Liszkay, Benjamin D. Engel, Till Rudack, Jan M. Schuller, Marc M. Nowaczyk
Summary: Auxiliary proteins play a crucial role in assisting the assembly of photosystems, as shown by the structure of a PSII assembly intermediate resolved by cryo-electron microscopy. This structure reveals mechanisms that protect against photodamage during vulnerable stages of biogenesis and prepare the active site for the unique water-splitting reaction catalyzed by the Mn4CaO5 cluster.
Article
Multidisciplinary Sciences
Tian Xie, Guangjun Xu, Yun Liu, Bradley Quade, Weichun Lin, Xiao-chen Bai
Summary: MuSK is a receptor tyrosine kinase that is essential for the neuromuscular junction. Its activation requires both agrin and LRP4 coreceptors. This study presents the cryo-EM structure of the extracellular ternary complex of agrin/LRP4/MuSK, revealing how LRP4 recruits agrin and MuSK to activate MuSK receptor.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Tian Xie, Guangjun Xu, Yun Liu, Bradley Quade, Weichun Lin, Xiao-chen Bai
Summary: MuSK is a receptor tyrosine kinase that plays a crucial role in the formation and maintenance of the neuromuscular junction. This study reveals the structure of the agrin/LRP4/MuSK ternary complex and uncovers the mechanism of how agrin and LRP4 coactivate the MuSK receptor.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Huirong Yang, Zishuo Yu, Xizi Chen, Jiabei Li, Ningning Li, Jiaxuan Cheng, Ning Gao, Hai-Xin Yuan, Dan Ye, Kun-Liang Guan, Yanhui Xu
Summary: The Tuberous sclerosis complex (TSC) consists of TSC1, TSC2, and TBC1D7 in a 2:2:1 stoichiometry, forming an arch-shaped structure. The complex includes two TSC1 coiled-coils and one TBC1D7 bridging over the TSC2 dimer, showing asymmetry. The TSC2 GAP domains are symmetrically cradled within the core module formed by TSC2 dimerization domain and central coiled-coil of TSC1, revealing mechanisms of TSC complex assembly and GAP activity.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yibo Jin, Paul K. Fyfe, Scott Gardner, Stephan Wilmes, Doryen Bubeck, Ignacio Moraga
Summary: IL-27 is an important cytokine that induces immunosuppressive responses. The study reveals the structure of the IL-27 receptor recognition complex and provides insights into the mechanism of IL-27 signaling. These findings contribute to a better understanding of IL-27's function and regulation.
Article
Biochemistry & Molecular Biology
Xizi Chen, Yanhui Xu
Summary: Researchers have made a breakthrough in determining the structure of TFIID-based PIC complexes, revealing the assembly mechanisms on TATA box and TATA-less promoters and providing a framework for further investigation of transcription initiation.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xiangnan Zhang, Yuan Xue, Zeyuan Guan, Chen Zhou, Yangfan Nie, She Men, Qiang Wang, Cuicui Shen, Delin Zhang, Shuangxia Jin, Lili Tu, Ping Yin, Xianlong Zhang
Summary: Cellulose is a abundant organic polymer in nature synthesized by cellulose synthases in the plasma membrane of higher plants. This study reports the structure of the homotrimeric CesA7 from Gossypium hirsutum, providing insight into how eukaryotic cellulose synthase assembles and offering a mechanistic basis for improving cotton fiber quality in the future.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Multidisciplinary Sciences
Meng Sun, Helin Zhang, Min Jiang, Yan Chai, Jianxun Qi, George F. Gao, Shuguang Tan
Summary: The crystal structures of TROP-2-ECD show that TROP-2 can form either cis or trans dimers, and can assemble into tetramers through distinct interfaces. Sacituzumab binds to a stretched polypeptide in the CPD region of TROP-2, which is not involved in cis- or trans-interactions. These findings provide insights into the molecular assembly of TROP-2 on tumor cells and potential implications for the design of biologics for tumor therapy.
Article
Cell Biology
Qiong Li, Yong-Liang Jiang, Ling-Yun Xia, Yuxing Chen, Cong-Zhao Zhou
Summary: This study elucidated the structure of an assembly intermediate of RuBisCO enzyme in cyanobacteria and discovered that the proteins Raf1 and RbcX facilitate and regulate RuBisCO assembly by controlling the formation of RuBisCO condensates.
Review
Plant Sciences
Etienne H. Meyer, James A. Letts, Maria Maldonado
Summary: This review summarizes recent findings on the structures of plant OXPHOS complexes, highlighting their plant-specific features and discussing their implications for our understanding of plant respiration and its regulation. The authors propose new hypotheses to be tested and suggest re-evaluating older findings. Further biochemical and structural research on the plant OXPHOS system will lead to a deeper understanding of plant respiration, with significant implications for agriculture, the environment, and society.
Article
Virology
Haining Li, Qi Liu, Luyuan Shao, Ye Xiang
Summary: African swine fever virus (ASFV) is the cause of a highly contagious and fatal disease in domestic pigs. The structure of ASFV is complex, with multiple layers and proteins involved in assembly. The crystal structure of the major protein fragment p150(oNC) of ASFV has been determined, revealing a unique triangular-shaped plate structure. Further analysis showed that p150 or a similar protein in faustovirus assembles into hexameric and pentameric capsomeres, forming the icosahedral inner capsids. These findings provide new insights into the assembly of ASFV inner capsid and offer a basis for understanding the assembly of nucleocytoplasmic large DNA viruses (NCLDV).
JOURNAL OF VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Xin Li, Guangyan Wu, Yin Yang, Shijuan Fu, Xiaofen Liu, Huimin Kang, Xue Yang, Xun-Cheng Su, Yuequan Shen
NATURE COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
Guangyan Wu, Shunjin Li, Guangning Zong, Xiaofen Liu, Shuang Fei, Linda Shen, Xiangchen Guan, Xue Yang, Yuequan Shen
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Guangyan Wu, Xue Yang, Yuequan Shen
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Zeng-zhi Yuan, Xiao-jie Yan, An-ding Zhang, Bo Chen, Yue-quan Shen, Mei-lin Jin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Jinxiu Zhang, Xue Yang, Zheng Wang, Hao Zhou, Xingqiao Xie, Yuequan Shen, Jiafu Long
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Cell Biology
Xingqiao Xie, Xiaojie Yan, Zheng Wang, Hao Zhou, Wentao Diao, Weihong Zhou, Jiafu Long, Yuequan Shen
JOURNAL OF MOLECULAR CELL BIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Xiaofen Liu, Guangyan Wu, Yi Yu, Xiaozhe Chen, Renci Ji, Jing Lu, Xin Li, Xing Zhang, Xue Yang, Yuequan Shen
Article
Biochemistry & Molecular Biology
Yingying Zhang, Yang Li, Xiao Liu, Jixue Sun, Xin Li, Jianping Lin, Xue Yang, Zhen Xi, Yuequan Shen
BIOCHEMICAL JOURNAL
(2020)
Article
Biochemistry & Molecular Biology
Meizi Liu, Dandan Wang, Yang Li, Xuemiao Li, Guangning Zong, Shuang Fei, Xue Yang, Jianping Lin, Xiaoqiang Wang, Yuequan Shen
Article
Multidisciplinary Sciences
Yue Ren, Tianlei Wen, Zhiqin Xi, Shunjin Li, Jing Lu, Xing Zhang, Xue Yang, Yuequan Shen
Article
Biochemistry & Molecular Biology
Muhammad Arif, Zitong Li, Qiong Luo, Luhua Li, Yuequan Shen, Shuzhen Men
Summary: BAG2 and BAG6 proteins in plants play diverse roles in response to various stresses, including abiotic and biotic. They exhibit differential sensitivity to ABA stress and survival rates in drought and heat stress, with varying levels of ROS accumulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Tianlei Wen, Ziyu Wang, Xiaozhe Chen, Yue Ren, Xuhang Lu, Yangfei Xing, Jing Lu, Shenghai Chang, Xing Zhang, Yuequan Shen, Xue Yang
Summary: CaSR is a crucial receptor for calcium homeostasis and hormone secretion. Study of its structures in different ligand-bound states provides insights into activation mechanism, allosteric modulation, and disease therapy. The negative allosteric modulator NPS-2143 induces structural rearrangement leading to an inactivated interface.
Review
Cell Biology
Yang Li, Xue Yang, Yuequan Shen
Summary: Orai channels, belonging to the CRAC channel family, play a crucial role in Ca2+ influx triggered by ER Ca2+ depletion. While structural and functional studies have advanced our understanding of Orai channel activation, the gating mechanism for Ca2+ permeation remains less well understood. This review summarizes existing structural studies, detailing features, comparing closed and open states, and proposing a push-pull model for Ca2+ permeation.
Article
Plant Sciences
Guichen Li, Zitong Li, Zeyun Yang, Yehoram Leshem, Yuequan Shen, Shuzhen Men
Summary: Research shows that mtHSC70-1 in Arabidopsis thaliana plays a crucial role in embryo development by modulating auxin biosynthesis and polar transport. Deficiency of mtHSC70-1 induces mitochondrial retrograde regulation, inhibiting auxin-related processes and resulting in defective embryo development.
Article
Biochemistry & Molecular Biology
Shijuan Fu, Luhua Li, Huimin Kang, Xue Yang, Shuzhen Men, Yuequan Shen
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)
