Article
Multidisciplinary Sciences
Li Li, Zuxian Xu, Ruipin Cao, Jiaxin Li, Chang-Jer Wu, Yinglu Wang, Hu Zhu
Summary: We investigated the influence of hydroxyl groups on the anti-quorum-sensing (anti-QS) and anti-biofilm activity of cyclic dipeptides against Pseudomonas aeruginosa PAO1. The presence of hydroxyl groups improved the inhibition of virulence factors and cytotoxicity, but reduced the ability to inhibit biofilm formation. These cyclic dipeptides interacted with the QS-related protein LasR, and the introduction of hydroxyl groups enhanced their self-assembly ability.
Article
Infectious Diseases
Tomasz Bogiel, Malgorzata Prazynska, Joanna Kwiecinska-Pirog, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska
Summary: The study compared the frequency of selected enzymatic virulence factor genes among non-duplicated carbapenem-resistant P. aeruginosa isolates, finding a higher prevalence of alkaline protease gene and a 37.4% detection rate for neuraminidase-1 gene. Significant differences in the prevalence of lasB and nan1 were observed between MBL-positive and MBL-negative strains.
Article
Biochemistry & Molecular Biology
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Agnieszka Mikucka
Summary: This study assessed the susceptibility and virulence factor gene frequency of clinical P. aeruginosa strains causing bloodstream infections. The results showed that these strains were resistant to multiple antibiotics and had diverse compositions of virulence factor genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecinska-Pirog, Eugenia Gospodarek-Komkowska
Summary: The study investigated the frequency and genotype distribution of 10 virulence factor genes in 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. Variations were observed in the prevalence and distribution of virulence genes, especially in terms of their carbapenemase synthesis ability and the strain origin.
Article
Microbiology
Gertrudis Horna, Joaquim Ruiz
Summary: Pseudomonas aeruginosa is an opportunistic pathogen that mainly affects severe patients in ICUs, characterized by high levels of antibiotic resistance and a long battery of virulence factors. Among these factors, T3SS plays a key role in injecting effectors into host cells and influencing immune responses to increase bacterial survival rates. Despite extensive research on T3SS, there are still questions and further points to be clarified or established. Efforts are being made to develop T3SS-targeting molecules to improve patient outcomes.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Microbiology
Qin Dou, Yuxiang Zhu, Chunhui Li, Zeran Bian, Huihui Song, Ruizhen Zhang, Yingsong Wang, Xile Zhang, Yan Wang
Summary: The combination of halogenated indoles and aminoglycoside antibiotics can enhance the effectiveness against Pseudomonas aeruginosa PAO1 and uncover the mechanism of the regulatory effect induced by 4F-indole.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Multidisciplinary
Eva Zahorska, Francesca Rosato, Kai Stober, Sakonwan Kuhaudomlarp, Joscha Meiers, Dirk Hauck, Dorina Reith, Emilie Gillon, Katharina Rox, Anne Imberty, Winfried Roemer, Alexander Titz
Summary: The researchers have successfully improved the solubility and stability of divalent galactosides and used them to inhibit the biological activity of Pseudomonas aeruginosa, which is important for the treatment of related infections.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Microbiology
Vijay K. K. Singh, Marianna Almpani, Kelsey M. M. Wheeler, Laurence G. G. Rahme
Summary: Pseudomonas aeruginosa is a common multidrug-resistant bacterial pathogen that poses a serious threat to critically ill and immunocompromised patients. Intestinal colonization by this pathogen is associated with increased mortality rates. In this study, the role of the three major communication systems in P. aeruginosa was investigated, and it was found that the MvfR system plays a key role in intestinal inflammation while the other systems have relatively minor effects. This study reveals the interrelationships of the quorum sensing systems during infection and provides important insights for developing effective antivirulence strategies.
Review
Chemistry, Medicinal
Qiaoqiang Li, Shen Mao, Hong Wang, Xinyi Ye
Summary: The survival pressure caused by antibiotics prompts bacteria to develop drug resistance. Quorum sensing is an intercellular communication system that regulates bacterial virulence and biofilm formation. In Pseudomonas aeruginosa, the formation and diffusion of biofilm is closely related to the quorum sensing system. Quorum-sensing inhibitors can reduce bacterial toxicity and enhance sensitivity to antibiotics, making them a popular topic for research and development in anti-infection. This review provides an overview of the Pseudomonas aeruginosa quorum-sensing system and quorum-sensing inhibitors research, which will aid in the future development of novel inhibitors.
Article
Gastroenterology & Hepatology
Guy Worley, David Burling, Alison Corr, Susan Clark, Rachel Baldwin-Cleland, Omar Faiz, John Jenkins
Summary: This study compared the effectiveness of MRI-enema with fluoroscopic WSCE in detecting anastomotic leaks, finding that MRI-enema provided greater accuracy and more anatomical detail. Patient experiences were similar, and there is potential for MRI-enema to play a significant role in the UK National Health Service.
COLORECTAL DISEASE
(2021)
Article
Multidisciplinary Sciences
Shuyu Li, Hengxi Sun, Jianghan Li, Yujiao Zhao, Ruiying Wang, Lei Xu, Chongyi Duan, Jialin Li, Zhuo Wang, Qinmeng Liu, Yao Wang, Songying Ouyang, Xihui Shen, Lei Zhang
Summary: Cyclic di-GMP transduces extracellular stimuli into intracellular responses to modulate important biological processes. Here, the authors show that AI-2 and bile salts induce cyclic di-GMP synthesis via YeaJ and YedQ, respectively, to repress the T3SS via a cyclic-di-GMP-responsive T3SS chaperone.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Microbiology
Donghoon Kang, Natalia V. Kirienko
Summary: Pseudomonas aeruginosa is a multidrug-resistant pathogen that causes acute infections in immunocompromised patients, using the siderophore pyoverdine to facilitate infection and virulence. Inhibiting pyoverdine production can reduce pathogen virulence and may be a promising therapeutic target.
Article
Multidisciplinary Sciences
Samuel L. Neff, Thomas H. Hampton, Charles Puerner, Liviu Cengher, Georgia Doing, Alexandra J. Lee, Katja Koeppen, Ambrose L. Cheung, Deborah A. Hogan, Robert A. Cramer, Bruce A. Stanton
Summary: Researchers have developed an application called CF-Seq, which allows users to filter studies and view differential gene expression analyses using a compendium of 128 studies and 1,322 samples.
Article
Multidisciplinary Sciences
Sachio Suzuki, Phawinee Subsomwong, Kouji Narita, Noriaki Kawai, Takahito Ishiai, Wei Teng, Rojana Sukchawalit, Akio Nakane, Sadatomo Tasaka, Krisana Asano
Summary: By comparing AbOMVs isolated under hypoxic and normoxic conditions, it was found that they have different protein profiles. While both AbOMVs had similar cytotoxic effects on bacteria, AbOMVs from normoxia induced higher levels of the cytokine TNF-alpha production and invasion of Staphylococcus aureus and Pseudomonas aeruginosa. On the other hand, AbOMVs from hypoxia significantly enhanced A. baumannii invasion into lung epithelial cells. This finding sheds light on the complex interactions between A. baumannii, coinfecting pathogens, and host cells mediated by OMVs, particularly the different pathogenic effects of AbOMVs under normoxic and hypoxic conditions.
Article
Gastroenterology & Hepatology
Marc A. Ward, Joseph F. Pierre, Raquel F. Leal, Yong Huang, Benjamin Shogan, Sushila R. Dalal, Christopher R. Weber, Vanessa A. Leone, Mark W. Musch, Gary C. An, Mrinalini C. Rao, David T. Rubin, Laura E. Raffals, Dionysios A. Antonopoulos, Mitch L. Sogin, Neil H. Hyman, John C. Alverdy, Eugene B. Chang
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2016)
Article
Biology
G. An, B. G. Fitzpatrick, S. Christley, P. Federico, A. Kanarek, R. Miller Neilan, M. Oremland, R. Salinas, R. Laubenbacher, S. Lenhart
BULLETIN OF MATHEMATICAL BIOLOGY
(2017)
Article
Microbiology
Sarah L. O'Brien, Sean M. Gibbons, Sarah M. Owens, Jarrad Hampton-Marcell, Eric R. Johnston, Julie D. Jastrow, Jack A. Gilbert, Folker Meyer, Dionysios A. Antonopoulos
ENVIRONMENTAL MICROBIOLOGY
(2016)
Article
Multidisciplinary Sciences
Adina Howe, Fan Yang, Ryan J. Williams, Folker Meyer, Kirsten S. Hofmockel
Article
Critical Care Medicine
Craig S. Brown, Jennifer R. Defazio, Gary An, Annemarie O'Connor, Emma Whitcomb, John Hart, Lawrence J. Gottlieb
JOURNAL OF BURN CARE & RESEARCH
(2017)
Article
Biotechnology & Applied Microbiology
Robert M. Bowers, Nikos C. Kyrpides, Ramunas Stepanauskas, Miranda Harmon-Smith, Devin Doud, T. B. K. Reddy, Frederik Schulz, Jessica Jarett, Adam R. Rivers, Emiley A. Eloe-Fadrosh, Susannah G. Tringe, Natalia N. Ivanova, Alex Copeland, Alicia Clum, Eric D. Becraft, Rex R. Malmstrom, Bruce Birren, Mircea Podar, Peer Bork, George M. Weinstock, George M. Garrity, Jeremy A. Dodsworth, Shibu Yooseph, Granger Sutton, Frank O. Gloeckner, Jack A. Gilbert, William C. Nelson, Steven J. Hallam, Sean P. Jungbluth, Thijs J. G. Ettema, Scott Tighe, Konstantinos T. Konstantinidis, Wen-Tso Liu, Brett J. Baker, Thomas Rattei, Jonathan A. Eisen, Brian Hedlund, Katherine D. McMahon, Noah Fierer, Rob Knight, Rob Finn, Guy Cochrane, Ilene Karsch-Mizrachi, Gene W. Tyson, Christian Rinke, Alla Lapidus, Folker Meyer, Pelin Yilmaz, Donovan H. Parks, A. M. Eren, Lynn Schriml, Jillian F. Banfield, Philip Hugenholtz, Tanja Woyke
NATURE BIOTECHNOLOGY
(2017)
Article
Biochemical Research Methods
Robert Chase Cockrell, Gary An
PLOS COMPUTATIONAL BIOLOGY
(2018)
Article
Biology
Gary An
BULLETIN OF MATHEMATICAL BIOLOGY
(2018)
Article
Medicine, Research & Experimental
Yoram Vodovotz, Gary An
WILEY INTERDISCIPLINARY REVIEWS-SYSTEMS BIOLOGY AND MEDICINE
(2019)
Article
Biochemical Research Methods
Tobias Paczian, William L. Trimble, Wolfgang Gerlach, Travis Harrison, Andreas Wilke, Folker Meyer
BMC BIOINFORMATICS
(2019)
Article
Virology
Chase Cockrell, Gary An
Summary: The study investigates the differences in immune responses between bats and humans, focusing on viral infections. Simulation experiments show that bats exhibit stronger viral tolerance, potentially offering crucial insights into disease severity in human viral infections.
Article
Health Care Sciences & Services
R. Laubenbacher, A. Niarakis, T. Helikar, G. An, B. Shapiro, R. S. Malik-Sheriff, T. J. Sego, A. Knapp, P. Macklin, J. A. Glazier
Summary: Digital twins, customized simulation models pioneered in industry, are being applied in medicine and healthcare with major successes. However, developing digital twins of the immune system presents challenges due to its complexity and measurement difficulties. This article outlines a roadmap for meeting these challenges and building a prototype of an immune digital twin.
NPJ DIGITAL MEDICINE
(2022)
Article
Biochemical Research Methods
Folker Meyer, Saurabh Bagchi, Somali Chaterji, Wolfgang Gerlach, Ananth Grama, Travis Harrison, Tobias Paczian, William L. Trimble, Andreas Wilke
BRIEFINGS IN BIOINFORMATICS
(2019)
Article
Chemistry, Physical
Jonathan Ozik, Nicholson Collier, Randy Heiland, Gary An, Paul Macklin
MOLECULAR SYSTEMS DESIGN & ENGINEERING
(2019)
Article
Computer Science, Cybernetics
Jonathan Ozik, Nicholson T. Collier, Justin M. Wozniak, Charles M. Macal, Gary An
IEEE TRANSACTIONS ON COMPUTATIONAL SOCIAL SYSTEMS
(2018)