Article
Biochemistry & Molecular Biology
Chung-Chun Wu, Ting-Ying Lee, Yu-Jhen Cheng, Der-Yang Cho, Jen-Yang Chen
Summary: Kaempferol efficiently inhibits the reactivation of EBV, making it a potential choice for anti-EBV therapy and cancer prevention.
Article
Biochemistry & Molecular Biology
Li Yuan, Shibing Li, Qiuyan Chen, Tianliang Xia, Donghua Luo, Liangji Li, Sailan Liu, Shanshan Guo, Liting Liu, Chaochao Du, Guodong Jia, Xiaoyun Li, Zijian Lu, Zhenchong Yang, Huanliang Liu, Haiqiang Mai, Linquan Tang
Summary: This study found that Epstein-Barr virus (EBV) infection reduces the sensitivity of nasopharyngeal carcinoma (NPC) cells to ferroptosis by activating the p62-Keap1-NRF2 signaling pathway and upregulating SLC7A11 and GPX4 expression. Inhibition of GPX4 enhances the chemosensitivity of EBV-infected NPC cells. High GPX4 expression is associated with poor clinical outcomes in NPC and other cancer types.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Pathology
Sudipta Samanta, Muthukaruppan Swaminathan, Jianing Hu, Khai Tuck Lee, Ajitha Sundaresan, Chuan Keng Goh, Chor Hiang Siow, Kwok Seng Loh, Soh Ha Chan, Joshua K. Tay, Ian Cheong
Summary: The use of immunofluorescence assay for detecting serum Epstein-Barr virus antibodies is the gold standard screening test for nasopharyngeal cancer in high-risk populations. This study demonstrates that integrating deep learning with automated fuzzy inference can improve the scalability and accuracy of NPC detection.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Li-Jen Liao, Wan-Lun Hsu, Chi-Ju Chen, Yen-Ling Chiu
Summary: Nasopharyngeal carcinoma is endemic in southern Asia and this article describes the molecular abnormalities in NPC and potential molecular mechanisms for future therapy. It provides an up-to-date review on the molecular pathways of NPC, improving our understanding of the disease and highlighting areas for further research.
Article
Virology
Kena Lin, Zhiwen Zeng, Xueqi Li, Wenjie Chen, Dongfeng Lin, Shanghang Xie, Zhicong Wu, Mengmeng Li, Sumei Cao, Jinlin Du
Summary: This study investigated the association between hypertension and Epstein-Barr virus (EBV) reactivation. It found that hypertension increased the risk of EBV reactivation, while beta-blockers and angiotensin converting enzyme inhibitors (ACEIs) were associated with a reduced risk of EBV reactivation.
Review
Oncology
Ramon Yarza, Mateo Bover, Maria Teresa Agullo-Ortuno, Lara Carmen Iglesias-Docampo
Summary: Nasopharyngeal carcinoma (NPC) is a molecularly paradigmatic tumor with complex environmental and host dependent factors, where Epstein Barr Virus (EBV) plays a key role in malignant transformation. The dysregulation of proteasome signaling is central in NPC pathogenesis and could be a potential therapeutic target. Studies have shown the efficacy of immunotherapy in NPC, with novel strategies aiming to reverse immune resistance and favor tumor recognition.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Review
Oncology
Xue Liu, Yayan Deng, Yujuan Huang, Jiaxiang Ye, Sifang Xie, Qian He, Yong Chen, Yan Lin, Rong Liang, Jiazhang Wei, Yongqiang Li, Jinyan Zhang
Summary: Genomic instability plays a crucial role in driving the evolution of malignancies, enhancing tumor cells' ability to adapt to the tumor microenvironment. Nasopharyngeal carcinoma progression is driven by genomic instability and persistent EBV infection, highlighting the importance of understanding and targeting genomic instability for effective treatment strategies.
Article
Oncology
Haiwen Chen, Xiaobing Duan, Xiaohong Deng, Yingping Huang, Xiang Zhou, Shanshan Zhang, Xiao Zhang, Pingjuan Liu, Chaopin Yang, Guojun Liu, Qinqin Ren, Yan Xiong, Bo Zhu, Jiexia Zhang, Tong Xiang
Summary: Nasopharyngeal carcinoma is an EBV-associated epithelial malignancy characterized by infiltration of lymphocytes, including NK cells. EBV-positive NPC cells often develop mechanisms to evade NK cell immune surveillance. Understanding the mechanisms involved in EBV-induced NK cell dysfunction may lead to the development of novel immunotherapies for NPC.
CANCER IMMUNOLOGY RESEARCH
(2023)
Article
Oncology
Kelly J. Yu, Wan-Lun Hsu, Chun-Ju Chiang, Tseng-Cheng Chen, Zhiwei Liu, Pei-Jen Lou, Scott Diehl, Alisa M. Goldstein, Chien-Jen Chen, Cheng-Ping Wang, Allan Hildesheim
Summary: This study reassessed familial aggregation of cancer in high-risk Taiwanese multiplex families with NPC cases and found evidence of NPC coaggregation within families, but not a broader familial syndrome involving NPC and other tumors. The study also observed elevated rates of secondary cancers among NPC cases, mostly head and neck and hematopoietic cancers, suggesting radiation treatment effects on subsequent cancer risk.
Article
Virology
Ting Shi, Linlin Huang, Zhengrong Chen, Jianmei Tian
Summary: This study investigated the characteristics of primary EBV infection and reactivation in children in Suzhou, China, finding that primary EBV infection was more common in children aged 36 to <72 months and often led to infectious mononucleosis. Reactivation of EBV mainly occurred in children aged 8 to <36 months and was identified as a risk factor for hemophagocytic lymphohistiocytosis (HLH). Among reactivated patients, HLH had a higher viral load compared to EBV infection and respiratory infection.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Review
Oncology
Rina Jiromaru, Takashi Nakagawa, Ryuji Yasumatsu
Summary: Nasopharyngeal carcinoma (NPC) has shown improved treatment outcomes in recent years, but advanced stages still carry a poor prognosis. Chemoradiotherapy is the main treatment option, along with platinum-based chemotherapy. Immunotherapy, especially immune checkpoint inhibitors, is being explored as a potential treatment for recurrent or metastatic NPC. Future trends in NPC treatment include risk classification based on plasma EBV-DNA levels and personalized therapy selection.
CANCER MANAGEMENT AND RESEARCH
(2022)
Article
Virology
Lingtong Huang, Xuan Zhang, Lisha Pang, Peng Sheng, Yanqiu Wang, Fan Yang, Huili Yu, Xiaohan Huang, Yue Zhu, Ning Zhang, Hongliu Cai, Lingling Tang, Xueling Fang
Summary: This study aimed to assess the landscape and clinical features of viral reactivation in the early onset of severe pneumonia in ICU patients. The most common reactivated viruses in the lower respiratory tract of patients with severe pneumonia were cytomegalovirus (CMV), herpes simplex virus-1 (HSV-1), and Epstein-Barr virus (EBV). Viral reactivation was associated with an increased risk of mortality.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Oncology
Qiao Li, TingTing Wang, Yan Huang, Qin Li, PeiYao Liu, Robert Grimm, CaiXia Fu, YunYan Zhang, Yajia Gu
Summary: This study aimed to investigate if EBV could produce differences on MRI and predict disease progression in NPC patients. MRI features and EBV status can be used as a tool for evaluating PD and developing tailored treatments targeting specific patient characteristics.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Xiandong Lin, Steven Wang, Keyu Lin, Jingfeng Zong, Qianlan Zheng, Ying Su, Tao Huang
Summary: This study investigated the roles of microRNAs and circular RNAs in Nasopharyngeal Carcinoma (NPC) by constructing a circRNA-miRNA-mRNA co-expression network and performing differential expression analysis. The Epstein-Barr virus (EBV) infection was found to be an important risk factor for NPC, with potential pathological differences between EBV+ and EBV- subtypes. The study identified differentially expressed coding and non-coding RNAs across different comparison groups, helping to elucidate genetic and epigenetic changes central to NPC progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Microbiology
Zhi Yi Su, Pui Yan Siak, Chee-Onn Leong, Shiau-Chuen Cheah
Summary: Nasopharyngeal carcinoma (NPC) is a metastasis-prone malignancy associated with Epstein-Barr virus (EBV). Despite global EBV infection, NPC incidences are higher in certain ethnic groups and endemic regions. Research has revealed the molecular mechanisms of NPC pathogenesis due to EBV infection and environmental/genetic factors. EBV-associated biomarkers can be used for mass population screening and EBV products may act as therapeutic targets. This review discusses the role of EBV in NPC and efforts to utilize EBV-associated molecules as biomarkers and therapeutic targets.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Oncology
Li-Chun Lu, Yi-Hsuan Lee, Chun-Jung Chang, Chia-Tung Shun, Chih-Yeu Fang, Yu-Yun Shao, Tsung-Hao Liu, Ann-Lii Cheng, Chih-Hung Hsu
Article
Oncology
Chung-Chun Wu, Chih-Yeu Fang, Sheng-Yen Huang, Shih-Hsin Chiu, Chia-Huei Lee, Jen-Yang Chen
Review
Immunology
Chih-Yeu Fang, Chia-Chyi Liu
EXPERT REVIEW OF VACCINES
(2018)
Correction
Multidisciplinary Sciences
Kuen-Haur Lee, Chi-Long Chen, Yi-Chao Lee, Tzu-Jen Kao, Kai-Yun Chen, Chih-Yeu Fang, Wen-Chang Chang, Yung-Hsaio Chiang, Chi-Chen Huang
SCIENTIFIC REPORTS
(2018)
Article
Dentistry, Oral Surgery & Medicine
Chih-Yeu Fang, Yun-Ho Lin, Chi-Long Chen
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2019)
Meeting Abstract
Biochemistry & Molecular Biology
Chih-Yeu Fang, Chi-Long Chen, Michael Hsiao
Meeting Abstract
Biochemistry & Molecular Biology
Chih-Yeu Fang, Michael Hsiao
Article
Cell Biology
Yu-Chan Chang, Yi-Fang Yang, Jean Chiou, Hsing-Fang Tsai, Chih-Yeu Fang, Chih-Jen Yang, Chi-Long Chen, Michael Hsiao
CELL DEATH & DISEASE
(2020)
Article
Cell Biology
Tsung-Ching Lai, Chih-Yeu Fang, Yi-Hua Jan, Hsiao-Ling Hsieh, Yi-Fang Yang, Chun-Yu Liu, Peter Mu-Hsin Chang, Michael Hsiao
CELL COMMUNICATION AND SIGNALING
(2020)
Review
Biochemistry & Molecular Biology
Chih-Yeu Fang, Tsung-Ching Lai, Michael Hsiao, Yu-Chan Chang
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Pharmacology & Pharmacy
Chih-Yeu Fang, Chia-Chyi Liu
Summary: Hand, foot, and mouth disease poses a serious threat to young children in the Asia-Pacific region, with enterovirus A71 (EV-A71) being a major cause of severe complications. While vaccines exist to prevent EV-A71 infection, there is a lack of effective treatments for other enteroviruses. Novel strategies are needed to develop new therapies for emerging enteroviruses associated with HFMD, including the use of DNA/RNA vaccine technology, structure biology, neutralization antibody studies, and nucleotide aptamer library screening.
EXPERT OPINION ON DRUG DISCOVERY
(2022)
Article
Biochemical Research Methods
Sheng-Chieh Lien, Chia-Chun Lu, Yu-Sheng Shen, Ya-Ting Yang, Shang-Rung Wu, Chih-Yeu Fang, Yen -Hung Chow, Ching -Len Liao, Jen -Ron Chiang, Chia-Chyi Liu
Summary: This study aimed to develop an approach to isolate and purify highly immunogenic mature EV-A71 particles. By applying a step gradient elution procedure, VP0-cleaved particles of EV-A71 were successfully isolated using Q-membrane anion-exchange chromatography. The purified VP0-cleaved particles exhibited characteristics equivalent to highly infectious F-particles of EV-A71.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Cell Biology
Chien-Hsiu Li, Chih-Yeu Fang, Ming-Hsien Chan, Chi-Long Chen, Yu-Chan Chang, Michael Hsiao
Summary: Based on large cohort survival analyses, this study found that FSTL3 is associated with a significantly worse prognosis in colorectal cancer (CRC) at the RNA and protein levels. Further analysis also showed that FSTL3 expression levels in the cytosol are significantly associated with poor overall and disease-free survival. Molecular simulation analysis revealed that FSTL3 participates in multiple cell motility signalling pathways to control CRC metastasis via the TGF-beta 1/TWIST1 axis.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Virology
Sheng-Chieh Lien, Yu-Sheng Shen, Hsiao-Yu Lin, Shang-Rung Wu, Chih-Yeu Fang, Chi-Hsun Chen, Yi-An Chen, Pele Choi-Sing Chong, Ming-Hsi Huang, Yen-Hung Chow, Jen-Ren Wang, Suh-Chin Wu, Chia-Chyi Liu
Summary: In this study, a serum-free HEK293A cell culture system was developed for the production of CVA10, achieving yields of over 108 TCID50/mL. The obtained CVA10 particles had similar biochemical and immunogenic properties as previous studies. Furthermore, it was found that CVA6 and A71 could also be easily propagated using this serum-free HEK293A cell culture system. These findings provide a solution for the propagation of CVA10 and the development of multivalent vaccines for HFMD prevention.
Article
Pathology
Chien-Hsiu Li, Chih-Yeu Fang, Ming-Hsien Chan, Pei-Jung Lu, Luo-Ping Ger, Jan-Show Chu, Yu-Chan Chang, Chi-Long Chen, Michael Hsiao
Summary: The study examined the relationship between JAM family members and different types of cancer using The Cancer Genome Atlas dataset. It found that mRNA levels of F11R in tumors were inversely correlated to the expression of JAM-2 and JAM-3, specifically in breast cancer (BCa), and this was associated with poor prognosis. The study also revealed that F11R expression significantly increased in HER2-enriched and basal-like BCa tumors.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2023)