Article
Medicine, Research & Experimental
Lays Fernanda Nunes Dourado, Lucas Gomes Oliveira, Carolina Nunes da Silva, Cibele Rodrigues Toledo, Silvia Ligorio Fialho, Rodrigo Jorge, Armando Silva-Cunha Junior
Summary: The study demonstrates that ketamine is safe for intravitreal use up to a dose of 0.118 mmol.L-1. It shows particular efficiency in protecting the retina from ischemic injury, reducing cell death, and promoting recovery of retinal function within safe dosage range.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Pharmacology & Pharmacy
Yuanfeng Zhu, Xiaoli Chen, Yongling Lu, Shijun Fan, Yongjun Yang, Qian Chen, Qianying Huang, Lin Xia, Yan Wei, Jiang Zheng, Xin Liu
Summary: This study reveals a previously unknown role of DPI in conferring protection against bacterial infection, suggesting a potential antimicrobial strategy involving modulation of CX43-dependent ATP leakage.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Cell Biology
Fangquan Chen, Shan Zhu, Rui Kang, Daolin Tang, Jiao Liu
Summary: In this study, the researchers identified ATP6V0D1 as a direct target of JTC801, which drives alkaliptosis in human PDAC cells. The interaction between ATP6V0D1 and STAT3 increases STAT3 expression and activity, maintaining lysosome homeostasis. Inhibition of STAT3 restores the sensitivity of ATP6V0D1-deficient cells to alkaliptosis. Furthermore, high expression of ATP6V0D1 correlates with prolonged survival in PDAC patients.
Article
Biology
Junyang Tan, Wenjun Wang, Xinjie Liu, Jinhong Xu, Yaping Che, Yanyan Liu, Jiaqiao Hu, Liubing Hu, Jianshuang Li, Qinghua Zhou
Summary: C11orf54 is a conserved ester hydrolase involved in DNA damage and repair. Knockdown of C11orf54 reduces cell proliferation and enhances cisplatin-induced DNA damage and apoptosis. The loss of C11orf54 leads to suppression of homologous recombination repair through decreased Rad51 expression.
COMMUNICATIONS BIOLOGY
(2023)
Article
Environmental Sciences
He Zhang, Zhefan Xie, Yongming Peng, Ailun Xie, Chunlai Fu, Dongyan Zheng, ZiWei Cai, Jiahong Zhong, Qiang Ming, Mingque Li, Renjian Lu, Xin Liu, Jialong Chen
Summary: The widely used pesticide rotenone can cause the loss of dopaminergic neurons and increase the risk of Parkinson's disease. However, there are few studies investigating the role of PARP1 in the inflammatory response and autophagy dysfunction induced by rotenone-induced neurodegeneration. This study found that overactivation of PARP1 caused by rotenone leads to autophagy dysfunction and NLRP3-mediated inflammation. Inhibition of PARP1 can reduce NLRP3-mediated inflammation and rescue rotenone-induced dopaminergic neurons loss, suggesting PARP1-TFEB-NLRP3 signaling as a potential treatment strategy for rotenone-induced neurodegeneration.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Yan Wang, Meiling Lian, Xiaoyu Xiu, Zhiwen Zhang, Liping Song, Shengzhou Wu
Summary: This study found that Dicer1 expression mediated amyloid peptide clearance by increasing APOE and blocking B2 RNA-mediated APOE mRNA degradation, leading to improved spatial memory in an animal model of Alzheimer's disease.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Nutrition & Dietetics
Shanshan Chen, Honghan Liu, Shimiao Wang, Hongbo Jiang, Le Gao, Lu Wang, Lesheng Teng, Chunyue Wang, Di Wang
Summary: The neuroprotective mechanism of Verbascoside (VB) was explored in this study. Proteomic analysis and immunological methods revealed that VB exerts its neuroprotective effect by inhibiting neuroinflammation and modulating the NF-kappa B-p65 pathway. These findings suggest that VB could be a potential candidate drug for the prevention and treatment of Alzheimer's disease (AD).
Article
Neurosciences
In-Young Choi, Jae Hyuk Shim, Mi-Hye Kim, Won Dong Yu, Yu Jin Kim, Gain Choi, Jae Ho Lee, Hee Jung Kim, Kyung-Ok Cho
Summary: During seizure-induced hippocampal necroptosis, an increase in truncated neogenin levels and a decrease in full-length neogenin levels were observed, along with an upregulation of phosphorylation of mixed lineage kinase domain-like pseudokinase. Treatment with DAPT can prevent neogenin truncation and protect neurons from NMDA-induced death.
Article
Neurosciences
Charles K. Davis, Saivenkateshkomal Bathula, Martin Hsu, Kahlilia C. Morris-Blanco, Anil K. Chokkalla, Soomin Jeong, Jeongwoo Choi, Shruti Subramanian, Jin Soo Park, Zsuzsanna Fabry, Raghu Vemuganti
Summary: The complex pathophysiology of post-traumatic brain damage may be effectively treated by a polypharmacological strategy, targeting multiple mechanisms simultaneously. A recent study on mice with traumatic brain injury (TBI) showed that a combination of three drugs resulted in improved motor and cognitive function, reduced secondary lesion volume, decreased oxidative DNA damage, and regulation of post-TBI inflammatory response.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Cheng Zeng, Jing Guo, Jiajia Wu, Tiantian Che, Xiaoping Huang, Huawen Liu, Zhenghong Lin
Summary: The E3 ubiquitin ligase HRD1 regulates autophagy and is involved in non-small cell lung carcinoma (NSCLC) metastasis. HRD1 inhibits autophagy by promoting ATG3 ubiquitination and degradation, leading to the release of MIEN1 and the promotion of NSCLC metastasis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Cell Biology
Ruzhou Zhao, Xingcheng Zhao, Xiaobo Wang, Yanqi Liu, Jie Yang, Shuai Jiang, Xiang Zhou, Bo Jiao, Lin Zhang, Yong Liu, Zhibin Yu
Summary: Fasting for 72 hours prior to extreme hypoxia exposure can reduce tissue injuries, maintain cardiac function, and improve survival rates in rats. Fasting reduces blood glucose levels and inhibits tissue mTOR activity, leading to decreased ATP consumption and increased reserves as well as improved mitochondrial oxygen utilization efficiency under acute hypoxia. Targeted regulation of mTOR could be a new strategy to enhance acute hypoxic tolerance in the body.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Huan Gu, Jie Peng, Meng Wang, Zimeng Guo, Haosu Huang, Lu Yan
Summary: This study identified two genes, miR-320-3p and MMP8, that are upregulated in mice with acute pancreatitis (AP). It investigated the involvement of these genes in AP development and explored the underlying mechanisms, offering a potential new approach for clinical diagnosis and treatment of AP.
MOLECULAR IMMUNOLOGY
(2022)
Article
Cell Biology
Xiaofeng Deng, Yu He, Xiongying Miao, Bo Yu
Summary: This study uncovered the promotive role of ATF4 in acute pancreatitis through upregulation of HDAC1, regulating NEP-mediated KLF4.
CELL DEATH & DISEASE
(2021)
Article
Pharmacology & Pharmacy
Feng Shi, Zhiwei Wang, Qi Wu, Xiaohan Zhong, Min Zhang, Bowen Li, Wei Ren, Shun Yuan, Yuanyang Chen
Summary: This study investigated the underlying mechanism of how iron deficiency (ID) promotes the formation of aortic media degeneration (AMD). The results showed that ID activates endoplasmic reticulum (ER) stress through mitochondrial oxidative stress, leading to increased apoptosis of aortic smooth muscle cells and the formation of AMD.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Neurosciences
Mina Borbor, Dongpei Yin, Ulf Brockmeier, Chen Wang, Marius Doeckel, Matthias Pillath-Eilers, Britta Kaltwasser, Dirk M. Hermann, Egor Dzyubenko
Summary: This study reveals that astrocytes play a crucial role in the maintenance of neuronal networks and their dysfunction can contribute to neurodegeneration. The researchers found that ischemia-reperfusion induces metabolic reprogramming in astrocytes, resulting in mitochondrial dysfunction and synaptic degeneration. Inhibiting STAT3 signaling pathway and increasing glycogen content in astrocytes can prevent synaptic degeneration.
Review
Cell Biology
Sara Santos Bernardes, Mauro Cunha Xavier Pinto, Jaime Henrique Amorim, Vasco Ariston de Carvalho Azevedo, Rodrigo Ribeiro Resende, Akiva Mintz, Alexander Birbrair
Summary: This article introduces a research finding that periostin expressed by pericytes in the glioma microenvironment is crucial for glioma angiogenesis. This new discovery will contribute to the development of therapies for gliomas.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Erica de Sousa, Marilia Ines Movio, Theo Henrique de Lima-Vasconcellos, Gabrieli Bovi dos Santos, Talita dos Santos Gomes, Lais Takata Walter, Daniela Almeida da Silva, Tiago Rodrigues, Giselle Cerchiaro, Alexandre Hiroaki Kihara
Summary: The regulation of VDAC1 plays a crucial role in neuronal survival and the activation of microglial cells. Inhibition of VDAC1 oligomerization has neuroprotective effects and prevents microglial polarization. These findings suggest that VDAC1 could be a potential therapeutic target in neuronal disorders.
CELL DEATH & DISEASE
(2022)
Review
Cell Biology
Cayo Almeida, Renan Paschoalino Pongilio, Marilia Ines Movio, Guilherme Shigueto Vilar Higa, Rodrigo Ribeiro Resende, Jianxiong Jiang, Erika Reime Kinjo, Alexandre Hiroaki Kihara
Summary: Temporal lobe epilepsy (TLE) is associated with oxidative stress and neuroinflammation, which involve various types of cells. Molecules involved in oxidative stress response and neuroinflammation have been proposed as potential targets for TLE treatment. This review examines the roles of Nicotinamide Adenine Dinucleotide Phosphate (NADPH) oxidase 2 (NOX2) and Myeloid Differentiation Primary Response 88 (MyD88) in astrocytes, microglia, and neurons during epileptogenesis. The use of single-cell RNA sequencing, transgenic technologies, and viral serotypes with specific promoters is also discussed for studying gene expression in different cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Marilia Ines Movio, Theo Henrique de Lima-Vasconcellos, Gabrieli Bovi dos Santos, Marcela Bermudez Echeverry, Elisabetta Colombo, Leonardo S. Mattos, Rodrigo Ribeiro Resende, Alexandre Hiroaki Kihara
Summary: Human-induced pluripotent stem cells (hiPSCs) have revolutionized the study of neurodegenerative diseases, including retinal dystrophies. In vitro modeling of retinal diseases using hiPSCs has offered new insights and possibilities for early diagnosis of conditions like Alzheimer's disease (AD) and Parkinson's disease (PD). This review highlights recent progress in the field and emphasizes the potential of retinal organoids obtained from potential patients to evaluate disease-related changes long before symptoms appear.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Lucas Felipe de Oliveira, Daniel Mendes Filho, Bruno Lemes Marques, Giovana Figueiredo Maciel, Ricardo Cambraia Parreira, Jose Rodrigues do Carmo Neto, Priscilla Elias Ferreira Da Silva, Rhanoica Oliveira Guerra, Marcos Vinicius da Silva, Helton da Costa Santiago, Alexander Birbrair, Alexandre H. Kihara, Valdo Jose Dias da Silva, Talita Glaser, Rodrigo R. Resende, Henning Ulrich
Summary: Infectious diseases have significant impacts on human health globally, and there is an urgent need for a better understanding. In vitro and in vivo infection models have made significant contributions to our current knowledge of these diseases. Organoids simulate tissue architecture and function, recapitulating many characteristics of human diseases and elucidating host-pathogen interactions in various systems. In this article, we discuss the applicability of organoid technology in modeling pathogenesis, host response, and features, which can be explored for the development of preventive and therapeutic treatments.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Bruno L. Marques, Giovana Figueiredo Maciel, Marcello R. Brito Junior, Lucas D. Dias, Sergio Scalzo, Anderson K. Santos, Alexandre Hiroaki Kihara, Helton da Costa Santiago, Ricardo C. Parreira, Alexander Birbrair, Rodrigo R. Resende
Summary: As the world population's life expectancy increases, more efficient therapies are needed for neurodegenerative disorders common in old age. Neural stem cells (NSCs) play a crucial role in the development and functioning of the nervous system and have broad therapeutic applications for neurodegenerative diseases. Understanding the mechanisms that govern NSC self-renewal, differentiation, and cell signaling is essential for these therapies.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Anderson K. Santos, Sergio Scalzo, Raysa T. V. de Souza, Pedro H. G. Santana, Bruno L. Marques, Lucas F. Oliveira, M. Filho Daniel, Alexandre Hiroaki Kihara, Helton da Costa Santiago, Ricardo C. Parreira, Alexander Birbrair, Henning Ulrich, Rodrigo R. Resende
Summary: Organoid development and organ-on-a-chip are technologies that utilize stem cells to create 3D multicellular structures resembling organs and tissues in vivo. They can be strategically employed for disease modeling, drug screening, and host-pathogen studies.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Luciana Simoes Rafagnin Marinho, Gabrielly Maria Denadai Chiarantin, Juliane Midori Ikebara, Debora Sterzeck Cardoso, Theo Henrique de Lima-Vasconcellos, Guilherme Shigueto Vilar Higa, Mariana Sacrini Ayres Ferraz, Roberto De Pasquale, Silvia Honda Takada, Fabio Papes, Alysson R. Muotri, Alexandre Hiroaki Kihara
Summary: The use of antidepressants during pregnancy can benefit the mother but has potential negative effects on neurodevelopment. In utero exposure to SSRIs has been linked to developmental abnormalities and an increased risk of ASD and ADHD. The development of hiPSC methods and cerebral organoids provides a valuable tool for studying the effects of these compounds on neurodevelopment. Single-cell RNA sequencing and electrophysiological measurements can be used to analyze the impact of antidepressants on developing neurons.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Behavioral Sciences
F. S. Borges, E. C. Gabrickc, P. R. Protachevicz, G. S. V. Higa, E. L. Lameu, P. X. R. Rodriguez, M. S. A. Ferraz, J. D. Szezech Jr, A. M. Batista, A. H. Kihara
Summary: Neuronal synchronization is crucial for brain communication and learning, but changes in connectivity can lead to hyper-synchronized states associated with epileptic seizures. The ARC protein is linked to synaptic alterations and epilepsy. Intense ARC immunoreactive neurons (IAINs) with increased connectivity and conductance are observed during status epilepticus, potentially contributing to abnormal seizure activity. This study investigates how IAINs connectivity influences firing patterns and synchronization. Synchronized burst patterns and intermittent up and down activities are observed in neural networks with increased IAINs connectivity. Optogenetics control effectively suppresses synchronized bursts when IAINs are targeted, highlighting their important role in both generating and suppressing highly synchronous activities.
EPILEPSY & BEHAVIOR
(2023)
Editorial Material
Biochemistry & Molecular Biology
Zhi Dong Zhou, Alexandre Hiroaki Kihara
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Miguel Tabanez, Ilma R. Santos, Juliane M. Ikebara, Mariana L. M. Camargo, Bianca A. Dos Santos, Bruna M. Freire, Bruno L. Batista, Silvia H. Takada, Rosanna Squitti, Alexandre H. Kihara, Giselle Cerchiaro
Summary: As people age, they become more susceptible to neurodegenerative illnesses such as Alzheimer's disease. This study found that the antioxidant hydroxytyrosol can regulate metal levels and improve learning performance in a rat model of AD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Rafael Bandeira Fabres, Debora Sterzeck Cardoso, Brian Aranibar Aragon, Bruna Petrucelli Arruda, Pamela Pinheiro Martins, Juliane Midori Ikebara, Alexander Drobyshevsky, Alexandre Hiroaki Kihara, Luciano Sturmer de Fraga, Carlos Alexandre Netto, Silvia Honda Takada
Summary: Oxygen deprivation is a major cause of mortality and morbidity in newborns, especially preterm infants. Surviving infants may develop neuropsychological pathologies, including learning difficulties and cerebral palsy. White matter injury, primarily affecting the myelin sheath, leads to long-term cognitive and motor impairments. Recent research has focused on the potential therapeutic targets of oligodendrocytes and myelination to minimize the impact of oxygen deprivation on the central nervous system. Furthermore, sexual dimorphism may influence neuroinflammation and white matter injury induced by oxygen deprivation.
MOLECULAR AND CELLULAR NEUROSCIENCE
(2023)
Article
Physics, Fluids & Plasmas
Mariana Sacrini Ayres Ferraz, Alexandre Hiroaki Kihara
Summary: The vast amount of available data requires efforts to extract meaningful information. In this study, different measurements of information entropy in binary series with temporal correlations are compared. A new measure called the binary permutation index (BPI) is proposed, which efficiently discriminates patterns in the series and offers advantages over previous methods.
