Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Chemistry, Multidisciplinary
Maigualida Cuenca, Maria Jose Marin, Lourdes Novoa, Ana O'Connor, Maria Carmen Sanchez, Juan Blanco, Jacobo Limeres, Mariano Sanz, Pedro Diz, David Herrera
Summary: The study aimed to investigate the subgingival microbiota in patients with Down syndrome, finding that those with periodontitis had higher levels of anaerobic bacteria, T. forsythia, and E. corrodens. Additionally, periodontitis patients were older, had lower rates of hypothyroidism, and higher levels of dental plaque.
APPLIED SCIENCES-BASEL
(2021)
Article
Ophthalmology
Jeremy J. Hoffman, John K. G. Dart, Surjo K. De, Nicole Carnt, Georgia Cleary, Scott Hau
Summary: This study evaluated the sensitivity and specificity of PCR, IVCM, and culture in diagnosing MK, finding IVCM to be the most sensitive technique for AK and FK diagnosis, while culture remains the gold standard for BK. With FK and AK becoming common in the UK, ophthalmology units need to have access to all these techniques to optimize their management of MK.
Article
Chemistry, Analytical
Dalton J. Nelson, Mindy Leelawong, Megan E. Pask, C. William Wester, Muktar H. Aliyu, Frederick R. Haselton
Summary: This report presents the first step towards a highly specific SNP detection method using magnetic bead processing and PCR detection, which is suitable for resource-limited clinics and allows for more effective clinical management of drug resistance mutations.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Nuli Xie, Huimin Wang, Jing Li, Kemin Wang, Jin Huang
Summary: A dual amplification strategy combining bio-barcode technique and auto-cycling primer extension is proposed for the sensitive detection of miRNA targets in complex biological samples. The strategy shows good sensitivity, with a detection limit of 50 fM, and can effectively distinguish different miRNAs, providing a new approach for the sensitive detection of nucleic acid targets.
CHEMICAL COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Sandile Phinda Songca
Summary: Nanozymes are synthetic nanoparticulate materials that mimic biological activities of enzymes, used for detection and identification of pathogens efficiently, offering cost-effectiveness and stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jae Jong Kim, Hyoung-Min Park, A. Young Kyoung, In Kyung Park, Si-Kyu Lim, Byoung Chul Park
Summary: Genetic mutations like SNP are common in genetic disorders and cancers, and PCR methods are widely used for SNP detection. The specifically designed PCR platform STexS can efficiently distinguish small amounts of SNP mixed in normal DNA, aiding in targeting potential SNPs or genetically mutated biomarkers in human clinical samples.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Priya Jarwar, Yar Muhammad Waryah, Muhammad Rafiq, Ali Muhammad Waryah
Summary: This study analyzed the association between SNP variants in CRYAA and CRYAB genes and Congenital Cataract. The results showed that the rs13053109 G > C variant in CRYAA gene is associated with increased risk of congenital cataract in infants. Haplotype analysis indicated weak linkage disequilibrium between the SNPs rs3761382, rs7278468, and rs13051039 of CRYAA gene. These findings suggest that the variation in CRYAA gene may be a risk factor for congenital cataract in infants.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Immunology
Ardian Ramadani, Benoit Coulin, Giacomo De Marco, Oscar Vazquez, Anne Tabard-Fougere, Nathaly Gavira, Christina N. Steiger, Romain Dayer, Dimitri Ceroni
Summary: This study aimed to report on the clinical course, biological parameters, and results of microbiological investigations among children with K. kingae-induced septic arthritis of the knee. The Kocher-Caird prediction algorithm is not sensitive enough to effectively detect K. kingae-induced septic arthritis of the knee, and nucleic acid amplification assays on oropharyngeal swabs and joint fluid are necessary to exclude this condition in young children presenting with knee effusion.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Kyung Hoon Kim, MinHo Yang, Younseong Song, Chi Hyun Kim, Young Mee Jung, Nam-Ho Bae, Sung-Jin Chang, Seok Jae Lee, Yong Tae Kim, Bong Gill Choi, Kyoung G. Lee
Summary: The development of a three-dimensional nanoweb film has enabled efficient capture of bacterial pathogens through hand-touching, demonstrating high capture efficiency and sensitivity. This unique nanostructure holds great potential for applications in capturing and detecting bacteria.
Article
Chemistry, Analytical
Younseong Song, Seongjin Lim, Yong Tae Kim, Yoo Min Park, Da Ae Jo, Nam Ho Bae, Seok Jae Lee, Bong Gill Choi, Sung Gap Im, Hyun Uk Kim, Kyoung G. Lee
Summary: Accurate analysis of dPCR images is crucial for miniaturizing dPCR system for point-of-care testing. In this study, a deep learning model combined with circle Hough transform was used to perform semantic segmentation and count droplets in low-quality dPCR images. The modified deep learning model achieved rapid, absolute quantification and accurate identification of target DNA, similar to the commercial dPCR system. The deep learning-based approach also demonstrated high sensitivity for detecting pathogenic bacteria from low-quality images. These findings lay the groundwork for efficient nucleic acid quantification and establishment of a point-of-care analysis platform.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Microbiology
Yu-Chieh Liao, Han-Chieh Wu, Ci-Hong Liou, Tsai-Ling Yang Lauderdale, I-Wen Huang, Jui-Fen Lai, Feng-Jui Chen
Summary: Molecular typing is widely used in laboratories and clinical settings. The accessibility of next-generation sequencing technologies is limited due to high capital cost. The MinION device from Oxford Nanopore Technologies offers real-time data analysis, rapid library preparation, and low cost per test, but has lower raw accuracy. In this study, a concise protocol for multilocus sequence typing of Staphylococcus aureus using multiplex polymerase chain reaction and the MinION device was developed. The effectiveness of the MinION sequencer in accurate and rapid molecular typing was demonstrated.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Melkamu Bedimo Beyene, Seifegebriel Teshome, A. Yehenew, Zewdu Terefework, Antoine R. Stuitje, Tamrat Abebe, Habtamu B. Beyene
Summary: This study assessed the performance of a novel real-time polymerase chain reaction (PCR) assay for detection of malaria infection and discrimination of Plasmodium species, showing high sensitivity and specificity.
Article
Engineering, Environmental
Amin Piri, Hyeong Rae Kim, Dae Hoon Park, Jungho Hwang
Summary: Airborne virus susceptibility is a major cause of severe respiratory diseases worldwide, emphasizing the need to develop rapid and accurate monitoring and diagnostic approaches for emerging infections. The study found that using a sampling solution of PBS+AA can effectively reduce oxidative damage to viral RNA and protein, thereby increasing virus survivability during sampling.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Immunology
Moritz Z. Kayser, Benjamin Seeliger, Christina Valtin, Jan Fuge, Stefan Ziesing, Tobias Welte, Mathias W. Pletz, Patrick Chhatwal, Jens Gottlieb
Summary: The study showed that BFPPp offered faster test results compared to conventional tests, which led to quicker clinical decisions. BFPPp demonstrated high accuracy in diagnosing viral LRTI, non-viral LRTI, and combined viral and non-viral LRTI. Additionally, preliminary therapies based on BFPPp results were upheld in 90% of cases.
TRANSPLANT INFECTIOUS DISEASE
(2022)
Article
Economics
Josephine Storch, Carolin Fleischmann-Struzek, Norman Rose, Thomas Lehmann, Anna Mikolajetz, Srikanth Maddela, Mathias W. Pletz, Christina Forstner, Ole Wichmann, Julia Neufeind, Monique Vogel, Konrad Reinhart, Horst Christian Vollmar, Antje Freytag
Summary: This study evaluated the effects of influenza and pneumococcal vaccinations on health care utilization and costs in the elderly using real-world data. Results showed that influenza vaccination was cost-saving in 2016, while pneumococcal vaccination led to lower outpatient care costs in the elderly in both 2015 and 2016.
EUROPEAN JOURNAL OF HEALTH ECONOMICS
(2022)
Letter
Respiratory System
Christina Bahrs, Miriam Kesselmeier, Martin Kolditz, Santiago Ewig, Gernot Rohde, Grit Barten-Neiner, Jan Rupp, Martin Witzenrath, Tobias Welte, Mathias W. Pletz
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Immunology
Nico Andreas, Sebastian Weis, Steffi Kolanos, Sabine Baumgart, Thomas Kamradt, Mathias W. Pletz
Summary: In this case report, a 64-year-old male participant of the CoNAN study was found to have a mild SARS-CoV-2 infection without any measurable antibody response. After vaccination with the ChAdOx1 vaccine 11 months later, no antibodies were detected in six serological tests. However, T-helper cell immunity, specifically SARS-CoV-2-specific Th cells, produced detectable amounts of IFN gamma and TNF six weeks after the infection. A robust T-cell immunity was detectable for at least six months after the infection and was further boosted by the vaccination. These findings suggest that assessing prior infection or vaccine response based solely on antibody detection may have limitations in individual patients.
Article
Microbiology
Janine Zoellkau, Juliane Ankert, Mathias W. Pletz, Sasmita Mishra, Gregor Seliger, Silvia M. Lobmaier, Clarissa U. Prazeres Da Costa, Vera Seidel, Katharina von Weizsaecker, Alexandra Jablonka, Christian Dopfer, Michael Baier, Thomas Horvatits, Ingrid Reiter-Owona, Tanja Groten, Benjamin T. Schleenvoigt
Summary: No increased risk of HEV exposure or serological signs of schistosomiasis or echinococcosis exposure could be observed in pregnant migrants in Germany. The anti-HEV serology status did not have an influence on fetal growth restriction.
Article
Microbiology
Gamal Wareth, Jorg Linde, Philipp Hammer, Mathias W. Pletz, Heinrich Neubauer, Lisa D. Sprague
Summary: The emergence of Klebsiella pneumoniae (K. pneumoniae) in German healthcare is concerning, as little is known about its presence in veterinary settings and food chains. This study investigated the antibiotic susceptibility and genomic determinants of 24 K. pneumoniae strains isolated from powdered milk samples produced in Germany. The findings revealed susceptibility to most antibiotics tested, but resistance to certain antibiotics was observed, along with the presence of genes associated with antimicrobial resistance and virulence. This poses a potential threat to public health, especially for neonates and infants consuming powdered milk.
Review
Microbiology
Kalysbek Kydyshov, Nurbolot Usenbaev, Almaz Sharshenbekov, Narynbek Aitkuluev, Murat Abdyraev, Salamat Chegirov, Jarkynay Kazybaeva, Hanka Brangsch, Falk Melzer, Heinrich Neubauer, Mathias W. Pletz
Summary: Brucellosis is still a challenge in developing countries like Kyrgyzstan, but the introduction of mass vaccination has contributed to its control.
Letter
Respiratory System
Mathias W. Pletz, Ralf Duerrwald, Janine Reiche, Norman Rose, Andre Scherag, Sebastian Weis
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Chemistry, Physical
Haodong Shen, Petra Roesch, Lara Thieme, Mathias W. Pletz, Juergen Popp
Summary: It was found that different metabolic states of bacteria contribute to the formation of biofilms, and there are differences in their chemical composition. Micro-Raman spectroscopy was used to compare and investigate five species of bacteria in exponential phase, stationary phase, and biofilm. Spectral differences revealed variations in chemical composition among different metabolic states. Additionally, different species and strains of bacteria exhibited distinct behaviors based on spectral differences and principal component analysis. Furthermore, a combination of principal component analysis and support vector machines was applied to distinguish bacterial species within the same metabolic states. This study provides valuable data for comparing bacteria in different metabolic states using micro-Raman spectroscopy and chemometrics models.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Multidisciplinary Sciences
Matthias Horn, Christian Theilacker, Ralf Sprenger, Christof von Eiff, Ernestine Mahar, Julia Schiffner-Rohe, Mathias W. Pletz, Mark van der Linden, Markus Scholz
Summary: Two next-generation pneumococcal conjugate vaccines (PCV15 and PCV20) have been licensed for use in adults, and PCV15 has also been licensed in children. A transmission model specific for Germany was developed to predict the prevalence of carriage and invasive pneumococcal disease (IPD) for serotypes included in these vaccines. The model predicts that the introduction of next-generation PCVs in adults may prevent a substantial and increasing proportion of adult IPDs, with PCV20 offering the broadest protection against pneumococcal disease.
Article
Infectious Diseases
David Attuy Vey da Silva, Ralf Dieckmann, Oliwia Makarewicz, Anita Hartung, Astrid Bethe, Mirjam Grobbel, Vitaly Belik, Mathias W. Pletz, Sascha Al Dahouk, Szilvia Neuhaus
Summary: Phenotypic susceptibility testing of ESBL and non-ESBL E. coli isolated from various sources showed differences in susceptibility to biocides and antimicrobials, with weakly positive correlations observed between biocide MICs/MBCs and antimicrobial MICs.
Letter
Respiratory System
Juliane Ankert, Stefan Hagel, Claudia Schwarz, Kaijie Pan, Liz Wang, Christof von Eiff, Bradford D. Gessner, Christian Theilacker, Mathias W. Pletz
Article
Infectious Diseases
Selma Tobudic, Christina Bahrs, Lisa Schneider, Emilia Paulussen, Lucie Bartonickova, Stefan Hagel, Peter Starzengruber, Heinz Burgmann, Mathias W. W. Pletz
Summary: This study aimed to compare treatment outcomes for bloodstream infections caused by a piperacillin/tazobactam-susceptible E. coli among three patient groups. The results showed that patients with BSI caused by AMP/SLB-resistant isolates treated with another monotherapy had an enhanced early treatment response compared to those treated with PIP/TAZ, indicating a potential association between initial use of PIP/TAZ and reduced treatment response.
Article
Infectious Diseases
Samira M. Hamed, Walid F. Elkhatib, Hanka Brangsch, Ahmed S. Gesraha, Shawky Moustafa, Dalia F. Khater, Mathias W. Pletz, Lisa D. Sprague, Heinrich Neubauer, Gamal Wareth
Summary: The genomic epidemiology and resistome structure of 46 A. baumannii clinical isolates were studied, revealing the extensive presence of resistance genes and their distribution among different global clones (GCs) in Egypt. This data is crucial for understanding A. baumannii infections and the dissemination of resistance genes.
Article
Cell Biology
Puja Kumari, Swathy O. Vasudevan, Ashley J. Russo, Skylar S. Wright, Victor Fraile-Agreda, Dylan Krajewski, Evan R. Jellison, Ignacio Rubio, Michael Bauer, Atsushi Shimoyama, Koichi Fukase, Yuanpeng Zhang, Joel S. Pachter, Sivapriya Kailasan Vanaja, Vijay A. Rathinam
Summary: This study demonstrates that extracellular vesicles can capture systemic bacterial lipopolysaccharide (LPS) and transfer it to the cytosol, triggering inflammatory responses and cell death.
NATURE CELL BIOLOGY
(2023)
