Article
Biochemistry & Molecular Biology
Celeste Caruso Bavisotto, Alessia Provenzano, Rosa Passantino, Antonella Marino Gammazza, Francesco Cappello, Pier Luigi San Biagio, Donatella Bulone
Summary: Similar to GroEL, Hsp60 is a folding machine that can stabilize aggregation-prone molecules by a holding-like mechanism. This study investigated the relationship between the oligomeric conformation of Hsp60 and its ability to inhibit fibrillization of Ab40 peptide.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Melina Theoni Gyparaki, Arian Arab, Elena M. Sorokina, Adriana N. Santiago-Ruiz, Christopher H. Bohrer, Jie Xiao, Melike Lakadamyali
Summary: The study found that tau forms small oligomers on microtubules ex vivo, which are different from those found in cells exhibiting tau aggregation and could be precursors of aggregated tau in pathology. Using an unsupervised shape classification algorithm that was developed, different tau phosphorylation states were associated with distinct tau aggregate species. The research elucidates tau's nanoscale composition under nonaggregated and aggregated conditions ex vivo.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Camilla B. Andersen, Yuichi Yoshimura, Janni Nielsen, Daniel E. Otzen, Frans A. A. Mulder
Summary: The study demonstrates that (-)-epigallocatechin gallate (EGCG) can reduce the toxicity of α-synuclein (α SN) oligomers by binding to both monomeric and oligomeric forms of α SN. EGCG binds to different regions of α SN with highest affinity for the monomeric form. Furthermore, kinetic data suggest a coupling between EGCG dimerization and α SN association.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biology
Haruka Narita, Tomohiro Shima, Ryo Iizuka, Sotaro Uemura
Summary: The N-terminal region of Argonaute2 can form amyloid-like aggregates, which may regulate the RNA silencing activity of DmAgo2.
Article
Biochemistry & Molecular Biology
Mantas Ziaunys, Andrius Sakalauskas, Tomas Sneideris, Vytautas Smirnovas
Summary: Protein aggregation into amyloid fibrils is associated with various disorders, but the mechanisms behind the conversion of non-harmful proteins into such aggregates are not fully understood. Recent studies suggest that multiple types of protein aggregates may co-exist in tissues of patients with amyloid-related disorders, potentially influencing each other.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Liya G. Bobyleva, Sergey A. Shumeyko, Elmira I. Yakupova, Alexey K. Surin, Oxana V. Galzitskaya, Hiroshi Kihara, Alexander A. Timchenko, Maria A. Timchenko, Nikita V. Penkov, Alexey D. Nikulin, Mariya Yu. Suvorina, Nikolay V. Molochkov, Mikhail Yu. Lobanov, Roman S. Fadeev, Ivan M. Vikhlyantsev, Alexander G. Bobylev
Summary: The research investigated the in vitro aggregation and amyloid properties of sMyBP-C, showing rapid formation of large aggregates and the formation of sMyBP-C oligomers consisting of 7-10 monomers. The results suggest a high aggregability of sMyBP-C in vitro and indicate the unlikeliness of pathological amyloid formation in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Ajamaluddin Malik, Javed Masood Khan, Abdullah S. Alhomida, Mohammad Shamsul Ola
Summary: Protein amyloid fibrils are supramolecular homopolymers that have various biological functions and connections to diseases such as type 2 diabetes. This study investigated the role of electrostatic interactions in surfactant-induced amyloid formation using insulin as a model protein. The results showed that SDS-induced amyloid formation was nucleation-independent at pH 2.0, and the solubilization was also instantaneous upon disruption of electrostatic interactions.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biochemistry & Molecular Biology
Francesca Laneri, Sara Garcia-Vinuales, Valeria Lanza, Nadia Licciardello, Danilo Milardi, Salvatore Sortino, Giuseppe Grasso
Summary: This article proposes dipyridamole as a new diagnostic tool and potential therapeutic agent in neurodegenerative diseases. It highlights the molecule's sensitivity to early stage amyloid formation and its ability to enhance ubiquitin chain growth.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Andrius Sakalauskas, Mantas Ziaunys, Ruta Snieckute, Vytautas Smirnovas
Summary: Flavones, a group of natural antioxidants, have been shown to impact the aggregation process of amyloidogenic proteins and peptides, with oxidized flavones demonstrating even greater potency in inhibiting aggregation. The position of hydroxyl groups on flavones plays a role in their autoxidation tendency and effectiveness as inhibitors against amyloid aggregation, showing potential for therapeutic applications.
Article
Biology
Federica Pizzo, Maria Rosalia Mangione, Fabio Librizzi, Mauro Manno, Vincenzo Martorana, Rosina Noto, Silvia Vilasi
Summary: This study investigates the role of the heat shock protein cpn60/cpn10 in insulin assembly and pathological aggregation. The results show that these proteins assist in the formation of insulin hexamers and prevent aberrant aggregation. This provides new insights into insulin assembly and related disorders.
Review
Biochemistry & Molecular Biology
Chuli Song, Tianyu Zhang, Yingjiu Zhang
Summary: Soluble aggregation and deposition of A beta 42 are the initial pathological hallmarks of Alzheimer's disease. Anti-A beta 42 antibodies can inhibit the aggregation and toxicity of A beta 42, but their epitope specificity affects their binding affinity for different A beta 42 species.
Article
Multidisciplinary Sciences
Mantas Ziaunys, Andrius Sakalauskas, Kamile Mikalauskaite, Vytautas Smirnovas
Summary: Protein aggregation is considered a major cause of several neurodegenerative diseases. Despite progress in amyloid research, the process of fibrillization remains not fully understood. Tracking insulin aggregation using ThT reveals anomalous double-sigmoidal kinetics, likely related to ThT-positive intermediates, which do not significantly alter the overall aggregation process.
Article
Biochemistry & Molecular Biology
Daniel Kachkin, Kirill V. Volkov, Julia Sopova, Alexander G. Bobylev, Sergei A. Fedotov, Sergei G. Inge-Vechtomov, Oxana Galzitskaya, Yury O. Chernoff, Aleksandr A. Rubel, Anna Y. Aksenova
Summary: This study assessed the amyloid properties of RAD51 protein in vitro and in a bacterial system. The purified human RAD51 protein formed amyloid-like aggregates with a cross-beta fibrillar structure, and were stained with amyloid-specific dyes. Cytoplasmic aggregates of RAD51 were also observed in cell cultures overexpressing RAD51.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Mohd Shahnawaz Khan, Faris Mohammad Alghadhiyyah, Mohammed Khaled Alhadeb, Yazeed Khalid Alhazzaa, Moneera Saud Al-Bagmi, Ghaliah S. Almutairi, Nojood Altwaijry, Majed S. Alokail
Summary: Protein aggregation occurs under abnormal conditions and can lead to deadly diseases. This study investigated the potential of hesperidin to inhibit the formation of insulin amyloid and found promising results.
JOURNAL OF MOLECULAR STRUCTURE
(2024)
Article
Biotechnology & Applied Microbiology
Md Shahnawaz, Tahmina Bilkis, Il-Seon Park
Summary: The Escherichia coli chaperonin DnaK can reduce or enhance the cytotoxicity of amyloid beta (Aβ) depending on its concentration. High ratios of DnaK to Aβ42 result in toxic oligomeric Aβ species accumulation and formation of complexes, while lower ratios lead to reduced cytotoxicity. DnaK is a useful tool for studying the ultrastructure formation and toxicity of Aβ peptide.
BIOTECHNOLOGY LETTERS
(2021)
Article
Chemistry, Physical
Payam Arghavani, Alireza Badiei, Seyyed Abolghasem Ghadami, Mehran Habibi-Rezaei, Faezeh Moosavi-Movahedi, Ladan Delphi, Ali Akbar Moosavi-Movahedi
Summary: This study reveals two distinct aggregation pathways for mTTR, resulting in the formation of either amorphous aggregates or well-organized fibrils. The pH and hydrophobicity of nanoparticles can modulate the aggregation of mTTR.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Review
Chemistry, Multidisciplinary
Mina Hajizadeh, Zainab Moosavi-Movahedi, Nader Sheibani, Ali A. Moosavi-Movahedi
Summary: This review investigates the importance of suicide enzyme inhibition in drug design, treatment, and industrial applications. The development of biomimetic artificial enzymes to prevent inhibition and the classification of suicide enzyme inactivation classes based on target enzyme groups were highlighted as key points to address severe diseases.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Nan-Nan Niu, Wen-Jun Zhao, Bao-Lin Xiao, Yu-Chen Liang, Xin Meng, Xin-Yan Song, Di Li, Jun Hong, Ali Akbar Moosavi-Movahedi
Summary: The study found that Q-Cyt c treatment at different pH values and quenching temperatures showed significant differences in catalytic efficiency, structural changes, and stability, which have important implications for their applications in various fields.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2022)
Article
Neurosciences
Matin Ramezani, Arman Zeinaddini Meymand, Fariba Khodagholi, Hamed Mohammadi Kamsorkh, Ehsan Asadi, Mitra Noori, Kimia Rahimian, Ali Saberi Shahrbabaki, Aisa Talebi, Hanieh Parsaiyan, Sepideh Shiravand, Niloufar Darbandi
Summary: This article reviews the impact of medicinal plants, particularly those rich in flavonoids, on cognitive dysfunction, learning and memory loss, and explores their underlying signaling pathways. It is suggested that flavonoids contribute to the improvement and prevention of learning, memory deficits, and cognitive dysfunction through the regulation of neurotransmission systems and enhancement of neurogenesis, synaptic plasticity, and neuronal survival.
NUTRITIONAL NEUROSCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Leila Khalili, Gholamreza Dehghan, Nader Sheibani, Alireza Khataee
Summary: The production of multifunctional lipid-polymer hybrid nanoparticles offers great opportunities for selective and efficient therapeutics delivery by combining the biomimetic behavior of liposomes and the advantages of polymers. Bio-modifications show immense potential in addressing the challenges of scaling up production and enhancing biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Neurosciences
Zahra Bahaeddin, Fariba Khodagholi, Forough Foolad, Fatemeh Emadi, Fatemeh Alijaniha, Shima Zareh Shahamati, Romina Tavassoli Yousef Abadi, Mohsen Naseri
Summary: The study found that consuming almonds during pregnancy can improve memory, reduce anxiety-like behavior, and enhance stress adaptation in adult offspring. Molecular assessments showed that almonds positively influenced cyclic AMP response element-binding proteins and brain-derived neurotrophic factor in the brain, while inhibiting the activity of certain enzymes.
NUTRITIONAL NEUROSCIENCE
(2023)
Review
Cell Biology
Yong-Seok Song, Andrew J. Annalora, Craig B. Marcus, Colin R. Jefcoate, Christine M. Sorenson, Nader Sheibani
Summary: Cytochrome P450 1B1 plays an important role in maintaining retinal iron homeostasis. By modulating iron levels, it influences oxidative stress and the development of ocular diseases.
Article
Cell Biology
Ismail S. Zaitoun, Yong-Seok Song, Hammam B. Zaitoun, Christine M. Sorenson, Nader Sheibani
Summary: This study established methods for visualizing choroidal macrophages, mast cells, and vasculature in the eyes of albino and pigmented mice, and found similarities between mice and humans in the arterial circle around the optic nerve. Different structural patterns of choriocapillaris were also observed. The findings contribute to the understanding and potential therapeutic targeting of ocular diseases affecting the choroid.
Article
Biochemistry & Molecular Biology
Yong-Seok Song, Ismail S. Zaitoun, Shoujian Wang, Soesiawati R. Darjatmoko, Christine M. Sorenson, Nader Sheibani
Summary: CYP1B1 expression in the retina is crucial for maintaining cellular redox homeostasis. Its metabolism of 17β-estradiol affects iron metabolism and lipid peroxidation, leading to iron accumulation and oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Sedigheh Abedanzadeh, Babak Karimi, Zainab Moosavi-Movahedi, Omid Pourshiani, Alireza Badiei, Ali A. Moosavi-Movahedi
Summary: Artificial enzymes resembling natural enzymes were developed using a rational design approach to simulate the coordination sphere around the iron active center. Periodic mesoporous organosilica (PMO) materials were used as supports due to their unique properties, and a new peroxidase-like nanozyme was fabricated. The biomimetic pathways used in this study showed potential for the development of nanozymes with excellent thermal stability and long-term storage stability.
MICROPOROUS AND MESOPOROUS MATERIALS
(2023)
Article
Cell Biology
Yong-Seok Song, Nasim Jamali, Christine M. M. Sorenson, Nader Sheibani
Summary: The integrity of retinal endothelial cell (EC) is crucial for maintaining retinal blood barrier and proper vision. Vitamin D, mainly mediated through the vitamin D receptor (VDR), plays a protective role in EC function. However, little is known about the impact of vitamin D-VDR on retinal EC function and ocular angiogenesis and inflammation. In this study, using retinal EC from VDR-deficient mice, the authors found that VDR expression is essential for endothelial cell-cell and cell-matrix interactions, as well as proliferation, migration, and inflammatory response of retinal EC. Furthermore, VDR deficiency leads to down-regulation of endothelial nitric oxide synthetase, enhanced hepcidin expression, and increased intracellular iron levels, contributing to the altered angiogenic and inflammatory state of retinal EC.
Article
Environmental Sciences
Nesa Hamidian, Gholamreza Dehghan, Samaneh Rashtbari, Alireza Khataee, Mehdi Khoobi, Nader Sheibani
Summary: A magnetite/Gd3+/beta-cyclodextrin nanoplatform was prepared and used for the degradation of tetracycline. The nanoplatform showed higher peroxidase-mimic activity than natural enzymes. It was able to degrade tetracycline efficiently and could be reused.
Article
Endocrinology & Metabolism
Subramanian Dharmarajan, Casandra Carrillo, Zhonghua Qi, Jonathan M. Wilson, Anthony J. Baucum II, Christine M. Sorenson, Nader Sheibani, Teri L. Belecky-Adams
Summary: This study aimed to investigate the impact of chronic inflammation on the loss of pericytes in early diabetic retinopathy. The results showed that both type 1 and type 2 diabetes led to similar progression of retinal pericyte loss and gliosis. However, the expression of inflammatory factors differed between the two diabetes models, with peak expression occurring at different ages. Additionally, retinal vascular changes were more severe in the type 2 diabetes model. Chronic exposure of retinal pericytes to IFN gamma resulted in decreased PDGFR beta signaling and increased levels of active PKC delta and CC3, indicating increased pericyte apoptosis.
Article
Chemistry, Physical
Mahdie Rahban, Parviz Norouzi, Zainab Moosavi-Movahedi, Ali A. Moosavi-Movahedi
Summary: This study introduces a new method, FFTCCV, for determining the reversibility of catalase activation upon injection of different concentrations of hydrogen peroxide (H2O2). The results show that catalase can rapidly decompose H2O2 at concentrations below 40 mM, but its activity gradually decreases at higher concentrations. The irreversibility of catalase is triggered at a concentration of 80 mM, and the catalase activity can be recovered at lower concentrations of H2O2 after multiple injections.
MOLECULAR CATALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Michele C. Larsen, Catherine M. Rondelli, Ahmed Almeldin, Yong-Seok Song, Alhaji N'Jai, David L. Alexander, E. Camilla Forsberg, Nader Sheibani, Colin R. Jefcoate
Summary: This study reveals the regulation of bone marrow cells by polycyclic aromatic hydrocarbon (PAH) pollutants and microbiome products through the aryl hydrocarbon receptor (AhR). The absence of Cyp1b1 and activation of AhR by PAH result in similar gene stimulations in these cells. Specific sources of Cyp1b1 are found to be linked to AhR and an OLFR network to modulate BM inflammation through diverse microbiome products.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)