Article
Engineering, Biomedical
Hongshi Ma, Chen Yang, Zhenjiang Ma, Xiaoyue Wei, Muhammad Rizwan Younis, Hanbo Wang, Wentao Li, Zhiyong Wang, Wenhao Wang, Yongxiang Luo, Peng Huang, Jinwu Wang
Summary: This study utilizes bioinspired calcium silicate nanowires and alginate composite hydrogels as building blocks to construct multiscale hierarchical bioactive scaffolds, and successfully promotes tissue regeneration from tendon to bone.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Thiago Domingues Stocco, Mayara Cristina Moreira Silva, Marcus Alexandre Finzi Corat, Gabriely Goncalves Lima, Anderson Oliveira Lobo
Summary: This study presents a new biofabrication strategy for developing biomimetic meniscus scaffolds. The use of bioprinted cell-laden hydrogel reinforced by aligned nanofiber sheets improved the structural integrity, shape fidelity, and mechanical properties of the scaffolds. Importantly, the introduction of nanofiber mats did not negatively affect cell viability. This bioengineering approach offers a promising strategy for tissue engineering of the knee meniscus.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Polymer Science
Evangelos Daskalakis, Boyang Huang, Cian Vyas, Anil Ahmet Acar, Ali Fallah, Glen Cooper, Andrew Weightman, Bahattin Koc, Gordon Blunn, Paulo Bartolo
Summary: This study proposes a method for preparing polycaprolactone/bioglass scaffolds with pore size gradient by investigating the coupled effect between material composition and scaffold architecture. The results show that the addition of bioglass improves the mechanical properties and biological performance of the scaffolds.
Article
Biochemistry & Molecular Biology
Tingkuo Chen, Haiming Jiang, Yibin Zhu, Xueliu Chen, Dao Zhang, Xiang Li, Fangcheng Shen, Hongyan Xia, Yonggang Min, Kang Xie
Summary: This study fabricated highly ordered 3D scaffolds for nerve cell culture through melt electrospinning writing, with the scaffold modified with double PDL and LM coatings after plasma treatment showing the most effective promotion of PC12 cell growth. The synergistic effect of PDL and LM on nerve cell growth was demonstrated, providing a versatile culture platform for growing nerve cells and recovering from peripheral nerve injury.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Seunghun S. Lee, Nicole Kleger, Gisela A. Kuhn, Helen Greutert, Xiaoyu Du, Thijs Smit, Andre R. Studart, Stephen J. Ferguson
Summary: Digital Light Processing is used to fabricate a hive-structured assemblable bespoke scaffold (HIVE) that can be manually assembled and customized for specific defect. It can be filled with different biological factors in different areas to provide site-specific treatments. Through in vitro and in vivo experiments, it has been shown to induce osteogenesis and angiogenesis in a controlled manner.
ADVANCED MATERIALS
(2023)
Article
Cell Biology
Walid Al-Akkad, Pilar Acedo, Maria-Giovanna Vilia, Luca Frenguelli, Alexander Ney, Irene Rodriguez-Hernandez, Peter L. Labib, Domenico Tamburrino, Gabriele Spoletini, Andrew R. Hall, Simone Canestrari, Anna Osnato, Jose Garcia-Bernardo, Leinal Sejour, Vessela Vassileva, Ioannis S. Vlachos, Giuseppe Fusai, Tu Vinh Luong, Steven R. Whittaker, Stephen P. Pereira, Ludovic Vallier, Massimo Pinzani, Krista Rombouts, Giuseppe Mazza
Summary: The tissue-matched human extracellular matrix (ECM) has a significant impact on driving the development of pancreatic ductal adenocarcinoma (PDAC) and promoting chemotherapy resistance, as revealed by this study. The 3D models provide a more reliable in vitro cell culture for primary and metastatic PDAC cells.
Article
Biochemistry & Molecular Biology
Nabila Mehwish, Yuan Chen, Muhammad Zaeem, Yi Wang, Bae Hoon Lee, Hui Deng
Summary: This study developed porous bovine serum albumin methacryloyl/gelatin methacryloyl (BG) biohybrid scaffolds for intraoral graft substitute with superb stability and excellent cell adhesion, as well as controlled release of Ibuprofen for postoperative recovery.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Endocrinology & Metabolism
Maria Heim, Ian J. Nixon, Elaine Emmerson, Anthony Callanan
Summary: Primary hypothyroidism significantly impacts patient's quality of life. Current standard treatment has limitations, and combination therapy is an alternative. Tissue engineering and regenerative medicine techniques have potential to restore thyroid function, but more research and clinical translation are needed.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Genetics & Heredity
Felix Boschann, Muhsin O. Cogulu, Davut Pehlivan, Saranya Balachandran, Pedro Vallecillo-Garcia, Christopher M. Grochowski, Nils R. Hansmeier, Zeynep H. Coban Akdemir, Cesar A. Prada-Medina, Ayca Aykut, Bjorn Fischer-Zirnsak, Simon Badura, Burak Durmaz, Ferda Ozkinay, Rene Haegerling, Jennifer E. Posey, Sigmar Stricker, Gabriele Gillessen-Kaesbach, Malte Spielmann, Denise Horn, Knut Brockmann, James R. Lupski, Uwe Kornak, Julia Schmidt
Summary: This study aimed to identify the genetic cause of a novel form of distal arthrogryposis through genomics analysis. The researchers discovered that rare biallelic recessive trait variants in the ADAMTS15 gene were associated with distal arthrogryposis syndrome.
GENETICS IN MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Martin Philipp Dieterle, Thorsten Steinberg, Pascal Tomakidi, Jiri Nohava, Kirstin Vach, Simon Daniel Schulz, Elmar Hellwig, Susanne Proksch
Summary: This study developed a new nonwoven scaffold for periodontal tissue engineering. Nonwovens of 16% gelatin/5% hydroxyapatite with additional porosity were produced by electrospinning and in situ cross-linking. The results showed that the scaffold efficiently supported the adhesion and survival of human mesenchymal stem cells (hMSCs) and periodontal ligament fibroblasts (PDLFs), indicating its potential applications.
Correction
Multidisciplinary Sciences
M. Suarez, E. Fernandez-Garcia, A. Fernandez, R. Lopez-Piriz, R. Diaz, R. Torrecillas
Summary: The paper has been amended and the revised version can be accessed through a link at the top of the paper.
SCIENTIFIC REPORTS
(2021)
Review
Biotechnology & Applied Microbiology
Tianze Sun, Jinzuo Wang, Huagui Huang, Xin Liu, Jing Zhang, Wentao Zhang, Honghua Wang, Zhonghai Li
Summary: The application of three-dimensional printing technology in the medical field has great potential for bone defect repair, especially personalized and biological repair. Low-temperature deposition manufacturing (LDM) is a promising type of rapid prototyping manufacturing and has been widely used to fabricate scaffolds in bone tissue engineering. The scaffolds fabricated by LDM have a multi-scale controllable pore structure and interconnected micropores, which are beneficial for the repair of bone defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Daichi Shigemizu, Yuya Asanomi, Shintaro Akiyama, Risa Mitsumori, Shumpei Niida, Kouichi Ozaki
Summary: Alzheimer's disease (AD) is a common neurodegenerative disease among elderly people. Genome-wide association studies (GWAS) have been successful in identifying genetic risk factors, but rare variants with potential large effects have not been thoroughly investigated. By using whole-genome sequencing, researchers identified two rare variants associated with AD and explored the functional mechanism of one variant. Additionally, gene-based association tests revealed other candidate genes related to AD.
MOLECULAR PSYCHIATRY
(2022)
Article
Polymer Science
Simonida Lj Tomic, Jasmina Nikodinovic-Runic, Marija Vukomanovic, Marija M. Babic, Jovana S. Vukovic
Summary: Hydrogel scaffolding biomaterials mimic the native extracellular matrix, and novel hydrogel scaffolds prepared using modified cryogelation show promising properties in terms of microstructure, mechanical strength, and biocompatibility. These newly synthesized hydrogel scaffolds have potential for versatile biomedical applications and tissue regeneration.
Article
Chemistry, Analytical
Yanan Wu, Zhijun Li, Mingqing Shi, Kun Yuan, Hong-Min Meng, Lingbo Qu, Zhaohui Li
Summary: A reconfigurable DNA hybridization-based reaction was developed to assemble DNAzyme computing for specific in vivo tumor imaging, demonstrating high specificity and sensitivity. This design may have broad applications in biological research and personalized medicine.
ANALYTICAL CHEMISTRY
(2021)
Review
Cardiac & Cardiovascular Systems
Andrew L. Durham, Mei Y. Speer, Marta Scatena, Cecilia M. Giachelli, Catherine M. Shanahan
CARDIOVASCULAR RESEARCH
(2018)
Editorial Material
Cell Biology
Daniel Brayson, Chin Yee Ho, Catherine M. Shanahan
JOURNAL OF CELL BIOLOGY
(2018)
Article
Cardiac & Cardiovascular Systems
Yana Dautova, Alexander N. Kapustin, Kevin Pappert, Matthias Epple, Hanneke Okkenhaug, Simon J. Cook, Catherine M. Shanahan, Martin D. Bootman, Diane Proudfoot
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2018)
Editorial Material
Urology & Nephrology
Adriana Dusso, Maria Isabel Colombo, Catherine M. Shanahan
KIDNEY INTERNATIONAL
(2018)
Article
Multidisciplinary Sciences
Ian Holt, Heidi R. Fuller, Le Thanh Lam, Caroline A. Sewry, Sally L. Shirran, Qiuping Zhang, Catherine M. Shanahan, Glenn E. Morris
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Lauren Porter, Rose-Marie Minaisah, Sultan Ahmed, Seema Ali, Rosemary Norton, Qiuping Zhang, Elisa Ferraro, Chris Molenaar, Mark Holt, Susan Cox, Samuel Fountain, Catherine Shanahan, Derek Warren
Article
Hematology
Malgorzata Furmanik, Rick van Gorp, Meredith Whitehead, Sadia Ahmad, Jayanta Bordoloi, Alexander Kapustin, Leon J. Schurgers, Catherine M. Shanahan
Summary: This study reveals that ER stress induces vascular calcification by increasing release of Grp78-loaded EVs, and for the first time links warfarin to promoting calcification by increasing EV release. The results of the study provide a novel mechanism of action for warfarin and the role of ER stress in cargo loading of EVs.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Hematology
Andrew M. Cobb, Syabira Yusoff, Robert Hayward, Sadia Ahmad, Mengxi Sun, Anja Verhulst, Patrick C. D'Haese, Catherine M. Shanahan
Summary: The study found that DNA damage stimulates Runx2 accumulation and enhances its activation of osteogenic target genes, leading to increased calcification. Runx2 also participates in DNA repair and its PARylation in response to genotoxic stress affects its localization at DNA lesions.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Editorial Material
Cardiac & Cardiovascular Systems
Meredith Whitehead, Catherine M. Shanahan
CARDIOVASCULAR RESEARCH
(2021)
Review
Engineering, Biomedical
Suzette T. Lust, Catherine M. Shanahan, Rebecca J. Shipley, Pablo Lamata, Eileen Gentleman
Summary: Cardiovascular diseases are often the result of pathological remodelling of blood vessels, driven by changes in vascular cell behaviors and abnormalities in ECM proteins. In vitro models of blood vessels can be useful for studying disease progression, with a focus on maintaining normal cell phenotypes, mimicking cell-ECM interactions, and promoting intercellular communication. By combining advancements in materials science, cell biology, fluidics, and modeling, it may be possible to create patient-specific blood vessel models for developing and testing therapies.
ACTA BIOMATERIALIA
(2021)
Editorial Material
Cardiac & Cardiovascular Systems
Marina Cecelja, Catherine M. Shanahan
CIRCULATION RESEARCH
(2021)
Review
Cardiac & Cardiovascular Systems
Pantelis Sarafidis, Sven Martens, Athanasios Saratzis, Daniella Kadian-Dodov, Patrick T. Murray, Catherine M. Shanahan, Allen D. Hamdan, Daniel T. Engelman, Ulf Teichgraber, Charles A. Herzog, Michael Cheung, Michel Jadoul, Wolfgang C. Winkelmayer, Holger Reinecke, Kirsten Johansen
Summary: Chronic kidney disease is an independent risk factor for abdominal aortic aneurysm, cardiovascular events, and all-cause mortality after AAA or thoracic aortic dissection surgery. Acute kidney injury (AKI) after aortic surgery occurs at a high rate and is linked to future cardiovascular events and mortality. The development of AKI and long-term kidney function differ based on the type of AAA intervention. Knowledge about AAA in the general population may not be applicable to CKD patients, who have higher co-morbidity prevalence and perioperative complication risks. This review summarizes the epidemiology, pathophysiology, diagnosis, and treatment of Aortic Diseases in CKD, highlighting knowledge gaps and areas of controversy for future research.
CARDIOVASCULAR RESEARCH
(2022)
Article
Multidisciplinary Sciences
Pamela Swiatlowska, Brian Sit, Zhen Feng, Emilie Marhuenda, Ioannis Xanthis, Simona Zingaro, Matthew Ward, Xinmiao Zhou, Qingzhong Xiao, Cathy Shanahan, Gareth E. Jones, Cheng-han Yu, Thomas Iskratsch
Summary: The study shows that vascular smooth muscle cells (VSMCs) undergo a phenotypic switch when exposed to a combination of hypertensive pressure and matrix compliance, which are prevalent during atherosclerosis. The molecular mechanism involves the regulation of the actin cytoskeleton through cofilin via distinct pathways.
Review
Hematology
Nadia R. Sutton, Rajeev Malhotra, Cynthia St. Hilaire, Elena Aikawa, Roger S. Blumenthal, Grace Gackenbach, Parag Goyal, Adam Johnson, Sagar U. Nigwekar, Catherine M. Shanahan, Dwight A. Towler, Brooke N. Wolford, Yabing Chen
Summary: Cardiovascular disease is the leading cause of death globally, particularly in individuals over the age of 65, with myocardial infarction and stroke being the primary contributors. The development of vascular aging and vascular calcification with aging is not fully understood, but is believed to be influenced by genetic risk, environmental factors, and the ability of vascular smooth muscle cells to acquire an osteogenic phenotype. Understanding the molecular mechanisms behind age-associated vascular pathology may lead to strategies for promoting healthy vascular aging. This article provides a summary of current knowledge on age-associated vascular disease, with a focus on vascular calcification.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Shanelle De Silva, Zhijuan Fan, Baoqiang Kang, Catherine M. Shanahan, Qiuping Zhang
Summary: Nesprins are multi-isomeric scaffolding proteins that play important roles in linking the nucleus to the cytoskeleton. Pathogenic mutations in nesprin-1 and-2 are associated with tissue-specific disorders, including dilated cardiomyopathy and Emery-Dreifuss muscular dystrophy. Recent evidence suggests that a small muscle-specific isoform of nesprin-1, called nesprin-1 alpha 2, may contribute to the muscle-specificity of these diseases. This review summarizes our current understanding of the muscle-specific functions of nesprin-1 and its isoforms, with the goal of providing insights into the potential pathological mechanisms and therapeutic targets of nesprin-related muscle diseases.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)